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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (4): 387-391.doi: 10.3736/jcim20080412

• Original Experimental Research • Previous Articles     Next Articles

Effects of Huoxue Qianyang Formula on expressions of proto-oncogenes c-fos and c-myc in spontaneous hypertensive rats with ventricular hypertrophy

De-yu Fu1(), Shi-hong Wang1, Duan Zhou2, Yu-ying Ma1, Lu Jin1, Liang-hua Zhu1   

  1. 1. Department of Cardiology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
    2. Department of Cardiology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2008-01-07 Online:2008-04-20 Published:2008-04-15
  • Contact: FU De-yu E-mail:fdy65@163.com

Objective: To explore the possible mechanism of Huoxue Qianyang Formula (HXQYF), a compound traditional Chinese herbal medicine, in reversing the left ventricular hypertrophy (LVH) of spontaneous hypertensive rats (SHRs) by analyzing the expressions of mRNAs and proteins of proto-oncogenes c-fos and c-myc in left ventricular muscle.
Methods: The experimental study was carried out in SHRs, the sex- and age-matched Wistar-Kyoto (WKY) rats were served as normal control (n=5, normal saline 10 ml/kg daily). Twenty-five SHRs were randomly divided into five groups: untreated group (n=5, normal saline 10 ml/kg daily), high-dose HXQYF-treated group (n=5, 0.84 g/ml HXQYF, 10 ml/kg daily), medium-dose HXQYF-treated group (n=5, 0.42 g/ml HXQYF, 10 ml/kg daily), low-dose HXQYF-treated group (n=5, 0.21 g/ml HXQYF, 10 ml/kg daily) and cilazapril-treated group (n=5, 1 mg/ml cilazapril, 10 ml/kg daily). The drugs were intragastrically administered once daily for 14 weeks. The expressions of mRNAs and proteins of proto-oncogenes c-fos and c-myc in left ventricular muscle were detected separately by in situ hybridization histochemical method and immunohistochemical method.
Results: Compared with the normal control group, the expressions of mRNAs and proteins of proto-oncogenes c-fos and c-myc in left ventricular muscle were significantly increased in untreated group (P<0.01). After treatment, the expressions of c-fos and c-myc mRNAs in left ventricular muscle in HXQYF-treated groups were significantly down-regulated as compared with those of the untreated group (P<0.05). The expressions of c-myc protein were also significantly decreased in high- and medium-dose HXQYF-treated groups as compared with the untreated group (P<0.05), but it had no significant effects in protein expression of c-fos in the three HXQYF-treated groups.
Conclusion: HXQYF can inhibit the expression of c-myc in ventricular hypertrophy tissue, which may be the mechanism in treating LVH of hypertention.

Key words: hypertrophy, left ventricular, rat, spontaneous hypertensive, Huoxue Qianyang Formula, protooncogene protein c-fos, proto-oncogene protein c-myc

CLC Number: 

  • R544.1

Table 1

Expression indexes of c-fos and c-myc mRNAs and proteins in left ventricular muscular tissues of SHRs ($\bar{x}$±s )"

Group n C-fos mRNA C-fos protein C-myc mRNA C-myc protein
Normal control 9 0.45±0.13△△ 0.49±0.22△△ 0.28±0.07△△ 0.30±0.06△△
Untreated 9 2.12±0.60** 1.49±0.42** 2.85±0.36** 2.37±0.28**
Cilazapril-treated 9 1.16±0.38* 0.79±0.14 1.36±0.34* 1.02±0.13*
Low-dose HXQYF-treated 9 1.15±0.36* 1.56±0.46* 1.45±0.31* 2.02±0.26**
Medium-dose HXQYF-treated 9 1.04±0.30* 0.97±0.33 1.12±0.14* 0.95±0.15*
High-dose HXQYF-treated 9 1.11±0.50* 1.01±0.45 1.29±0.38* 1.08±0.15*
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