活血潜阳方对自发性高血压大鼠心肌肥厚组织原癌基因c-fos和c-myc表达的影响

doi:10.3736/jcim20080412

Abstract

Objective: To explore the possible mechanism of Huoxue Qianyang Formula (HXQYF), a compound traditional Chinese herbal medicine, in reversing the left ventricular hypertrophy (LVH) of spontaneous hypertensive rats (SHRs) by analyzing the expressions of mRNAs and proteins of proto-oncogenes c-fos and c-myc in left ventricular muscle.
Methods: The experimental study was carried out in SHRs, the sex- and age-matched Wistar-Kyoto (WKY) rats were served as normal control (n=5, normal saline 10 ml/kg daily). Twenty-five SHRs were randomly divided into five groups: untreated group (n=5, normal saline 10 ml/kg daily), high-dose HXQYF-treated group (n=5, 0.84 g/ml HXQYF, 10 ml/kg daily), medium-dose HXQYF-treated group (n=5, 0.42 g/ml HXQYF, 10 ml/kg daily), low-dose HXQYF-treated group (n=5, 0.21 g/ml HXQYF, 10 ml/kg daily) and cilazapril-treated group (n=5, 1 mg/ml cilazapril, 10 ml/kg daily). The drugs were intragastrically administered once daily for 14 weeks. The expressions of mRNAs and proteins of proto-oncogenes c-fos and c-myc in left ventricular muscle were detected separately by in situ hybridization histochemical method and immunohistochemical method.
Results: Compared with the normal control group, the expressions of mRNAs and proteins of proto-oncogenes c-fos and c-myc in left ventricular muscle were significantly increased in untreated group (P<0.01). After treatment, the expressions of c-fos and c-myc mRNAs in left ventricular muscle in HXQYF-treated groups were significantly down-regulated as compared with those of the untreated group (P<0.05). The expressions of c-myc protein were also significantly decreased in high- and medium-dose HXQYF-treated groups as compared with the untreated group (P<0.05), but it had no significant effects in protein expression of c-fos in the three HXQYF-treated groups.
Conclusion: HXQYF can inhibit the expression of c-myc in ventricular hypertrophy tissue, which may be the mechanism in treating LVH of hypertention.

Key words: hypertrophy, left ventricular, rat, spontaneous hypertensive, Huoxue Qianyang Formula, protooncogene protein c-fos, proto-oncogene protein c-myc

CLC Number:

R544.1

De-yu Fu, Shi-hong Wang, Duan Zhou, Yu-ying Ma, Lu Jin, Liang-hua Zhu. Effects of Huoxue Qianyang Formula on expressions of proto-oncogenes c-fos and c-myc in spontaneous hypertensive rats with ventricular hypertrophy. Journal of Chinese Integrative Medicine, 2008, 6(4): 387-391.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20080412

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Group	n	C-fos mRNA	C-fos protein	C-myc mRNA	C-myc protein
Normal control	9	0.45±0.13^△△	0.49±0.22^△△	0.28±0.07^△△	0.30±0.06^△△
Untreated	9	2.12±0.60**	1.49±0.42**	2.85±0.36**	2.37±0.28**
Cilazapril-treated	9	1.16±0.38*^△	0.79±0.14^△	1.36±0.34*^△	1.02±0.13*^△
Low-dose HXQYF-treated	9	1.15±0.36*^△	1.56±0.46*	1.45±0.31*^△	2.02±0.26**
Medium-dose HXQYF-treated	9	1.04±0.30*^△	0.97±0.33	1.12±0.14*^△	0.95±0.15*^△
High-dose HXQYF-treated	9	1.11±0.50*^△	1.01±0.45	1.29±0.38*^△	1.08±0.15*^△

Effects of Huoxue Qianyang Formula on expressions of proto-oncogenes c-fos and c-myc in spontaneous hypertensive rats with ventricular hypertrophy

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