SUBSCRIBE
Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (12): 1347-1352.doi: 10.3736/jcim20111211

• Original Experimental Research • Previous Articles     Next Articles

Effects of Chinese herbal medicine Xinfeng Capsule on expressions of platelet-activating factor and interleukins 6 and 17 in peripheral blood of rats with adjuvant arthritis

Rui-kai Zong1, Jian Liu2()   

  1. 1. 2009 Grade of Doctoral Postgraduate Class, Hubei University of Traditional Chinese Medicine, Wuhan 430065, Hubei Province, China
    2. Department of Rheumatism and Immunity, the First Hospital, Anhui College of Traditional Chinese Medicine, Hefei 230031, Anhui Province, China
  • Received:2011-07-14 Accepted:2011-08-09 Online:2011-12-20 Published:2011-12-15

RichHTML

1

PDF (PC)

43

Like

Objective: To observe the levels of platelet-activating factor (PAF), interleukin-6 (IL-6) and IL-17 in peripheral blood of rats with adjuvant arthritis (AA), and the effects of Xinfeng Capsule (XFC), a compound traditional Chinese herbal medicine.
Methods: A total of 40 male Sprague-Dawley rats were randomized into normal control group, model group, methotrexate (MTX) group, Tripterygium Wilfordii polycoride Tablet (TPT) group, and XFC group, respectively. AA was induced in rats by intracutaneous injection of 0.1 mL Freund's complete adjuvant in the right hindlimb. Contents of PAF, IL-6 and IL-17 in peripheral blood were measured by enzyme-linked immunosorbent assay. Body weight of rats, paw swelling and arthritis index (AI) were also observed.
Results: Compared with the normal control group, the levels of PAF, IL-6 and IL-17 in peripheral blood, paw swelling and AI were significantly increased in the model group (P<0.01). Compared with the model group, levels of PAF, IL-6 and IL-17, paw swelling and AI of the three drug-treated groups were significantly decreased (P<0.01). The body weight of the XFC group was significantly higher than those of the MTX- and TPT-treated groups. Compared with the MTX- and TPT-treated groups, level of PAF in the XFC group was significantly increased (P<0.05). The level of PAF in peripheral blood of the AA rats was positively correlated with IL-6 and IL-17 levels in peripheral blood, paw swelling and AI (P<0.01).
Conclusion: The expression of PAF increases in peripheral blood of rats with AA, and it correlates with IL-6, IL-17, paw swelling and AI. XFC can decrease the expression of PAF, restrain infection induced by the activation of platelets, decrease the levels of IL-6 and IL-17, and hence decrease the paw swelling of rats with AA.

Key words: adjuvant arthritis, peripheral blood, platelet-activating factor, interleukin-6, interleukin-17, drugs, Chinese herbal

Table 1

Contents of platelet-activating factor, interlaukin-6 and interlaukin-17 in peripheral blood of rats in each group (x±s, ng/L)"

Group n Platelet-activating factor Interleukin-6 Interleukin-17
Control 8 14.02±2.48 16.41±0.95 58.90±9.97
Model 8 33.71±6.31** 20.52±0.83** 84.78±8.89**
Methotrexate 8 23.92±6.40△△ 17.16±1.16△△ 65.13±7.98△△
TPT 8 22.47±5.05△△ 17.11±1.19△△ 64.87±5.59△△
XFC 8 16.97±4.89△△▲□ 17.02±1.28△△ 62.47±9.05△△

Table 2

Body weight of rats in each group (x±s, g)"

Group n Body weight
Before phlegmasia Before treatment After treatment
Control 8 214.38±18.98 260.00±11.02 360.25±23.46
Model 8 211.88±17.31 226.25±35.73* 260.50±37.20**
Methotrexate 8 213.75±13.02 231.88±30.70* 311.00±43.61*△△
TPT 8 212.50±11.95 230.00±14.88* 317.63±32.51*△△
XFC 8 213.75±22.80 232.50±13.63* 351.88±21.96△△▲□

Table 3

Paw swelling and arthritis index of rats in each group (x±s)"

Group n Paw swelling (%) Arthritis index
Before treatment After treatment Before treatment After treatment
Control 8 33.28±10.90 38.94±24.69 0.00±0.00 0.00±0.00
Model 8 65.99±16.49** 79.20±24.59** 7.63±0.92** 8.13±0.64**
Methotrexate 8 65.09±14.11** 46.00±14.94△△ 7.25±1.04** 4.00±0.93**△△
TPT 8 62.59±20.19** 44.92±20.37△△ 7.13±1.13** 3.88±1.25**△△
XFC 8 68.86±16.41** 41.57±15.28△△ 7.88±0.64** 3.50±1.07**△△
[1] Quinn M, Fitzgerald D. Platelet function: Assessment, diagnosis, and treatment. Totowa, New Jersey, USA: Humana Press. 2005 : 86.
[2] Feng F, Ding ZJ, Liu J . The study on the model of adjuvant arthritis in rats. Tianjin Yao Xue. 2004; 16 (2) : 1-4. Chinese with abstract in English.
冯芳, 丁志建, 刘俊 . 佐剂性关节炎大鼠模型的实验研究.天津药学. 2004; 16(2):1-4.
[3] Xu SY, Bian RL, Chen X. Methodology of pharmacological experiments. 3rd ed. Beijing: People’s Medical Publishing House. 2002: 919-921. Chinese.
徐叔云, 卞如濂, 陈修 . 药理实验方法学.第3版.北京: 人民卫生出版社. 2002: 919-921.
[4] Zhang JT. Modern experimental methods in pharmacology. Beijing : Joint Publishing House of Beijing Medical University and Peking Union Medical College. 1998 : 1383. Chinese.
张钧田 . 现代药理实验方法.北京: 北京医科大学中国协和医科大学联合出版社. 1998: 1383.
[5] Sun LJ, Li F, Yan YL, Zhang FX, Tao JM . Expression of platelet-activating factor and platelet parameters in rheumatoid arthritis. Shandong Yi Yao. 2009 ; 49(3): 70-71. Chinese.
孙丽君, 李芳, 闫永龙, 张风肖, 陶杰梅 . 血小板活化因子及血小板参数在类风湿关节炎中的表达及意义. 山东医药. 2009; 49(3):70-71.
[6] Firestein GS, Zvaifler NJ . How important are T cells in chronic rheumatoid synovitis?: H. T cell-independent mechanisms from beginning to end. Arthritis Rheum. 2002; 46(2) : 298-308.
doi: 10.1002/(ISSN)1529-0131
[7] Miranda-Carus ME, Balsa A, Benito-Miguel M, Perez de Ayala C, Martin-Mola E . IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate.[J] Immunol. 2004; 173(2):1463-1476.
doi: 10.4049/jimmunol.173.2.1463
[8] Choy E . Clinical experience with inhibition of interleukin-6. Rheum Dis Clin North Am. 2004; 30(2) : 405-415, viii.
doi: 10.1016/j.rdc.2004.01.008 pmid: 15172049
[9] Honorati MC, Cattini L, Facchini A . IL-17, IL-lbeta and TNF-alpha stimulate VEGF production by dedifferentiated chondrocytes. Osteoarthritis Cartilage. 2004; 12(9) : 683-691.
doi: 10.1016/j.joca.2004.05.009
[10] Yoshitake F, Itoh S, Narita H, Ishihara K, Ebisu S . Interleukin-6 directly inhibits osteoclast differentiation by suppressing receptor activator of NF-kappaB signaling pathways.[J] Biol Chem. 2008; 283(17):11535-11540.
doi: 10.1074/jbc.M607999200
[11] Yang Y, Hou JS, Chi HJ, Fan CY, Han M . Effects of Astragalus mongholicus on airway inflammation in the rat pulmonary tissue of chronic obstructive pulmonary disease. Shandong Yi Yao. 2010; 50(20): 49-51. Chinese.
doi: 10.3969/j.issn.1002-266X.2010.20.019
杨月, 侯继申, 池红井, 范常艳, 韩梅 . 黄芪对慢性阻塞性肺病大鼠气道炎症干预作用实验研究.山东医药. 2010; 50(20) : 49-51.
doi: 10.3969/j.issn.1002-266X.2010.20.019
[12] Ji XJ, Li A, Duan ML, Zhang SW . The effect of astragaloside W on NF-icB and glucocorticoid receptor in monocytes in external inflammation state. Zhongguo Lin Chuang Yi Xue. 2010 ; 17 (4): 590-593. Chinese with abstract in English.
doi: 10.3969/j.issn.1008-6358.2010.04.047
冀晓俊, 李昂, 段美丽, 张淑文 . 黄芪甲苷对体外炎症状态下单核细胞NF-kB及糖皮质激素受体表达的影响.中国临床医学. 2010; 17(4):590-593.
doi: 10.3969/j.issn.1008-6358.2010.04.047
[13] Ren XR, Fan HC, Wei JZ, Ji N, Liang LY, Ma CG . Efects of triptolide on TNF-«, IL-12 and TGF-pi in spinal cord of EAE mice. Zhongguo Dang Dai Yi Yao. 2010 ; 17(15) : 5-6. Chinese with abstract in English.
doi: 10.3969/j.issn.1674-4721.2010.15.003
任晓荣, 樊红翠, 尉杰忠, 纪宁, 梁丽云, 马存根 . 雷公藤内酯醇对EAE小鼠脊髓TNF-a､IL-12和TGF-pi 表达的影响.中国当代医药. 2010; 17(15):5-6.
doi: 10.3969/j.issn.1674-4721.2010.15.003
[14] Yang F, Bai XJ, Liu KJ, Yang YJ, Zeng Y . Effect of triptolide on secretion of inflammatory cellular factor NO and IL-6 in mice peritoneal macrophage activated by lipopolysaccharide. Yi Yao Dao Bao. 2010; 29(1): 9-12. Chinese with abstract in English.
doi: 10.3870/yydb.2010.01.003
杨帆, 白祥军, 刘开俊, 杨业金, 曾叶 . 雷公藤内酯醇对内毒素激活小鼠腹腔巨噬细胞分泌促炎递质NO和 IL-6 的影响.医药导报. 2010; 29(1):9-12.
doi: 10.3870/yydb.2010.01.003
[1] Shriniwas S. Basaiyye, Sanjay Kashyap, Kannan Krishnamurthi, Saravanadevi Sivanesan. Induction of apoptosis in leukemic cells by the alkaloid extract of garden cress (Lepidium sativum L.). Journal of Integrative Medicine, 2019, 17(3): 221-228.
[2] Khanh Nguyen, Jean Sparks, Felix O. Omoruyi. Investigation of the cytotoxicity, antioxidative and immune-modulatory effects of Ligusticum porteri (Osha) root extract on human peripheral blood lymphocytes. Journal of Integrative Medicine, 2016, 14(6): 465-472.
[3] Qi-ming Xue, Lu Huang, Ning Li. Effects of electroacupuncture at Tianshu (ST25) on pro- and anti-inflammatory cytokines in rats with severe acute pancreatitis. Journal of Chinese Integrative Medicine, 2011, 9(6): 658-664.
[4] Lei Chen, Xiao-ying Bi, Li-xun Zhu, Yi-qing Qiu, Su-ju Ding, Ben-qiang Deng. Flavonoids of puerarin versus tanshinone ⅡA for ischemic stroke: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2011, 9(11): 1215-1220.
[5] Qi-lin Fu, Yi-yang Hu, Qin Feng, Xiao-ning Wang, Jing-hua Peng, Tuan Cui. Analysis of major herbs in Chinese herbal formula Jianpi Huoxue Decoction for improving intestinal permeability based on uniform design. Journal of Chinese Integrative Medicine, 2011, 9(11): 1234-1241.
[6] Hua-qiang Ouyang, Lu-ming Liu, Zhen Chen, Jian-min Luo, Er-xin Yu. Effects of Qingyi Huaji decoction on serum levels of interleukin-6, interleukin-8 and tumor necrosis factor-α in nude mice bearing pancreatic tumors. Journal of Chinese Integrative Medicine, 2010, 8(7): 655-661.
[7] Ying Shen , Ji-di Lu . Treatment of adjuvant arthritis in rats with Chinese herbal fumigation: Efficacy and mechanism. Journal of Chinese Integrative Medicine, 2009, 7(2): 157-162.
[8] Xin-ling Li, Fang Lian, Yan-he Liu. Effects of Quyu Jiedu Granule on expressions of tumor necrosis factor-α and interleukin-6 mRNAs in ovarian granulosa cells of endometriosis rats. Journal of Chinese Integrative Medicine, 2008, 6(9): 960-963.
[9] Wan-sen Sun, Zhu Wang, Juan Wang, Qiao-ya Ma, Xiao-qiang Zhang, Xi-li Wu. Effects of Qufeng Tongluo Recipe on proliferation of and TGF-β1 and IL-6 mRNA expressions in glomerular mesangial cells from rats. Journal of Chinese Integrative Medicine, 2008, 6(9): 915-920.
[10] Xiao-bing Qu, Shui-ping Zhao, Jing Xu, Li-ni Dong. Effects of curcumin on secretion of adiponectin and interleukin-6 in human adipose tissues: an in vitro study. Journal of Chinese Integrative Medicine, 2008, 6(7): 711-715.
[11] Jian Liu, Xue-fang Yu, Rui-kai Zong. Effects of Xinfeng Capsule on behaviors and myocardial ultrastructure in adjuvant arthritis rats. Journal of Chinese Integrative Medicine, 2008, 6(6): 606-610.
[12] Shao-Ying Lou, Yi Liu, Wei-Hua Chen, Jian Ying, Yan-Ming He, Wen-jian Wang. Pollen Typhae total flavones inhibit expression of interleukin-6 in C2C12 skeletal muscle cells cultured with palmitate. Journal of Chinese Integrative Medicine, 2008, 6(5): 488-491.
[13] Yong-hong Li, Zong-wen Huang, Ping Xue, Jia Guo, Fu-qian He, Zheng You, Zheng-rong Wang. Effects of Chaiqin Chengqi Decoction on activation of nuclear factor-κB in pancreas of rats with acute necrotizing pancreatitis. Journal of Chinese Integrative Medicine, 2008, 6(2): 180-184.
[14] Tan Li, Wei Wang, Tong Li. Antileukemic mechanism of resveratrol in vitro and in mice bearing L1210-tumor. Journal of Chinese Integrative Medicine, 2008, 6(12): 1267-1274.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228
Journal of Integrative Medicine, ISSN: 2095-4964 CN 31-2083/R
Copyright © Journal of Integrative Medicine
Powered by Beijing Magtech Co. Ltd
沪公网安备31010602003416号      沪ICP备18017633号-3