Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2012, Vol. 10 ›› Issue (7): 800-806.doi: 10.3736/jcim20120711

• Original Experimental Research • Previous Articles     Next Articles

Effects of Chinese herbal medicine Neiyi Recipe-medicated serum on angiopoiesis of endometriosis in the chick chorioallantoic membrane model

Ling Xu1, Qiao-ling Zhou1, Jie Han1, Dan-ying Zhang1, Hui-juan Dong1, Zai-long Cai2, Chao-qin Yu1(), Dong-xia Zhai1()   

  1. 1. Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
    2. Experimental Research Center, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
  • Received:2011-11-14 Accepted:2011-12-20 Online:2012-07-20 Published:2018-07-15
  • Contact: Yu Chao-qin,Zhai Dong-xia E-mail:chqyu81@gmail.com;zhaidongx@163.com

Objective: To compare angiopoiesis ability of eutopic and ectopic endometrial tissue isolated from women with endometriosis and endometrium isolated from women without endometriosis (control), and to explore the inhibitory effects of medicated serum of Neiyi Recipe, a compound traditional Chinese herbal medicine.
Methods: Chick chorioallantoic membrane (CAM) model of endometriosis was established by transplanting endometrium onto CAM. The CAMs were then hatched with blank serum or medicated serum of danazol or Neiyi Recipe, which were prepared in rats by orally administering. The sizes of the transplanted tissue and new vessels around the transplanted tissue were measured. Expression of vascular endothelial growth factor (VEGF) was detected by immunohistochemical method.
Results: There was no difference in the sizes of transplanted tissue among CAM models of ectopic and eutopic endometrial tissue isolated from women with endometriosis or control (P>0.05), and more new vessels were found around the ectopic and eutopic endometrial tissue than the endometrial tissue of control (P<0.05). Compared with the controls, the size of the transplanted tissue and positive area of new vessels were significantly inhibited by Neiyi Recipe-medicated serum (P<0.01, P<0.05), and similar changes happened in the danazol groups, except for the size of transplanted tissue from ectopic endometrial tissue (P>0.05). Expression of VEGF was significantly higher in eutopic and ectopic endometrial tissue than in the control (P<0.01); the level of VEGF obviously reduced in the Neiyi Recipe and danazol groups (P<0.01), but no significant difference was detected between them.
Conclusion: Endometrium from women with endometriosis stimulates the formation of new vessels by increase the expression of VEGF. Neiyi Recipe-medicated serum significantly decreases the expression of VEGF in eutopic and ectopic endometrial tissues and thus restrains the formation of new vessels, reduces the blood supply and inhibits growth of ectopic endometrial tissue, which are similar to danazol, but has greater efficacy in suppressing the expression of VEGF.

Key words: endometriosis, chorioallantoic membrane, vascular endothelial growth factor, drugs, Chinese herbal, angiogenesis inhibitors

Table 1

Sizes of transplanted tissue and changes after intervention in different groups (x±s)"

Group n Size of transplanted tissue (cm2
Blank control 5 1.176±0.527
Eutopic control 5 1.502±0.211
Eutopic Neiyi Recipe serum 5 0.730±0.185△△
Eutopic danazol serum 5 0.728±0.144△△
Ectopic control 5 1.240±0.218
Ectopic Neiyi Recipe serum 5 0.517±0.201
Ectopic danazol serum 5 0.660±0.363

Table 2

Area of new vessels around the transplanted tissue in different groups (x±s)"

Group n Area of new vessels (%)
Blank control 4 9.84±0.89
Eutopic control 4 12.40±1.90
Eutopic Neiyi Recipe serum 4 6.03±0.4△△
Eutopic danazol serum 4 6.08±0.73△△
Ectopic control 4 13.12±1.87*
Ectopic Neiyi Recipe serum 4 6.79±1.47▲▲
Ectopic danazol serum 4 6.06±2.11▲▲

Table 3

Positive expression index and area ratio of VEGF in different groups (x±s)"

Group n Positive area ratio (%) Optical density Positive expression index
Blank control 6 4.57±1.97 12.00±1.67 56.44±28.37
Eutopic control 6 16.33±1.39 15.33±1.03 249.62±15.53**
Eutopic Neiyi Recipe serum 6 5.61±2.04 12.67±0.82 69.91±22.21△△
Eutopic danazol serum 6 4.51±0.94 13.00±0.63 58.80±14.03△△
Ectopic control 6 17.97±4.22 17.83±2.59 320.39±82.12**
Ectopic Neiyi Recipe serum 6 5.60±0.89 12.83±0.98 71.74±11.86△△□
Ectopic danazol serum 6 11.02±0.66 14.50±0.84 160.00±15.77

Figure 1

VEGF expression in CAM planting focal observed by immunohistochemical method (Light microscopy, ×200) A: Blank control group; B: Eutopic control group; C: Ectopic control group; D: Eutopic Neiyi Recipe serum group; E: Eutopic danazol serum group; F: Ectopic Neiyi Recipe serum group; G: Ectopic danazol serum group. VEGF: vascular endothelial growth factor; CAM: chorioallantoic membrane."

[1] Yu CQ, Shi SF, Liu YH, Wang RX, Song YH, Yu J . Primary culture and morphologic observation of eutopic and ectopic endometrial cells from patients with endomehiosis[J]. J Chin Integr Med, 2006,4(2):189-193
俞超芹, 石书芳, 刘玉环, 王瑞霞, 宋艳华, 俞瑾 . 人子宫内膜异位症在位和异位内膜细胞原代培养及形态学观察[J]. 中西医结合学报, 2006,4(2):189-193
[2] Gaetje R, Kotzian S, Herrmann G, Baumann R, Starzinski-Powitz A . Invasiveness of endometriotic cells in vitro[J]. Lancet, 1995,346(8988):1463-1464
doi: 10.1016/S0140-6736(95)92474-4 pmid: 7490993
[3] Amalinei C, Caruntu ID, Giusca SE, Balan RA . Matrix metalloproteinases involvement in pathologic conditions[J]. Rom J Morphol Embryol, 2010,51(2):215-228
doi: 10.1071/RD10084 pmid: 20495735
[4] Matsuzaki S, Maleysson E, Darcha C . Analysis of matrix metalloproteinase-7 expression in eutopic and ectopic endometrium samples from patients with different forms of endometriosis[J]. Hum Reprod, 2010,25(3):742-750
doi: 10.1093/humrep/dep435
[5] Juhasz-Bss I, Hofele A, Lattrich C, Buchholz S, Ortmann O, Malik E . Matrix metalloproteinase messenger RNA expression in human endometriosis grafts cultured on a chicken chorioallantoic membrane[J]. Fertil Steril, 2010,94(1):40-45
doi: 10.1016/j.fertnstert.2009.02.052
[6] Sharpe-Timms KL, Penney LL, Zimmer RL, Wright JA, Zhang Y, Surewicz K . Partial purification and amino acid sequence analysis of endometriosis protein-Ⅱ(ENDO-Ⅱ) reveals homology with tissue inhibitor of metalloproteinases-1(TIMP-1)[J]. J Clin Endocrinol Metab, 1995,80(12):3784-3787
[7] McLaren J . Vascular endothelial growth factor and endometriotic angiogenesis[J]. Hum Reprod Update, 2000,6(1):45-55
doi: 10.1093/humupd/6.1.45 pmid: 10711829
[8] Han YH, Zhou YF, Zheng SR . Study on the expression of vascular endothelial growth factor in patients with adenomyosis of the uterus[J]. Zhonghua Fu Chan Ke Za Zhi, 2002,37(9):539-541
doi: 10.3760/j.issn:0529-567X.2002.09.009
韩燕华, 周应芳, 郑淑蓉 . 子宫腺肌病患者血管内皮生长因子表达的研究[J]. 中华妇产科杂志, 2002,37(9):539-541
doi: 10.3760/j.issn:0529-567X.2002.09.009
[9] Tan XJ, Lang JH, Liu DY, Shen K, Leng JH, Sun DW, Liu ZF, Zhu L, Xu XY . Expression of vascular endothelial growth factor and thrombospondin-1 in the ectopic and eutopic endometrium of women with endometriosis[J]. Zhonghua Fu Chan Ke Za Zhi, 2001,36(12):727-730
doi: 10.3760/j.issn:0529-567X.2001.12.007
谭先杰, 郎景和, 刘东远, 沈铿, 冷金花, 孙大为, 刘珠凤, 朱兰, 许秀英 . 血管内皮生长因子及血小板反应素在异位子宫内膜组织中的表达[J]. 中华妇产科杂志, 2001,36(12):727-730
doi: 10.3760/j.issn:0529-567X.2001.12.007
[10] Shifren JL, Tseng JF, Zaloudek CJ, Ryan IP, Meng YG, Ferrara N, Jaffe RB, Taylor RN . Ovarian steroid regulation of vascular endothelial growth factor in the human endometrium: implications for angiogenesis during the menstrual cycle and in the pathogenesis of endometriosis[J]. J Clin Endocrinol Metab, 1996,81(8):3112-3118
[11] Lin J, Gu Y . Effect of monocyte chemoattractant protein-1 and estradiol on the secretion of vascular endothelial growth factor in endometrial stromal cells in vitro[J]. Fertil Steril, 2005,84(6):1793-1796
doi: 10.1016/j.fertnstert.2005.03.090
[12] Kressin P, Wolber EM, Wodrich H , Meyhöfer-Malik A, Buchweitz O, Diedrich K, Malik E .Vascular endothelial growth factor mRNA in eutopic and ectopic endometrium[J]. Fertil Steril, 2001,76(6):1220-1224
doi: 10.1016/S0015-0282(01)02898-9 pmid: 11730754
[13] Yu CQ, Zhai MF, Yao RM, Hu ZZ . Effect of Nei-Yi recipe on plasma β-endorpin level during menstrual cycle in women with endometriosis[J]. Zhongguo Zhong Xi Yi Jie He Za Zhi, 1995,15(1):6-8
doi: 10.7661/CJIM.1995.1.6
俞超芹, 翟美芙, 姚韧敏, 胡增珍 . 子宫内膜异位症患者性周期β-内啡肽变化及中药内异方对其的影响[J]. 中国中西医结合杂志, 1995,15(1):6-8
doi: 10.7661/CJIM.1995.1.6
[14] Yu CQ, Liu YH, Peng XP, Li J, Pan RP, Ling CQ . IL-8 and IL-10 levels in endometriosis and regulated role of Neiyifang on them[J]. Zhongguo Zhong Xi Yi Jie He Za Zhi, 2000,20(8):603-605
doi: 10.7661/CJIM.2000.8.603
俞超芹, 刘玉环, 彭新萍, 李瑾, 潘瑞萍, 凌昌全 . 子宫内膜异位症白细胞介素-8与白细胞介素-10水平及内异方对其调节作用[J]. 中国中西医结合杂志, 2000,20(8):603-605
doi: 10.7661/CJIM.2000.8.603
[15] Yu C, Cai Z, Liu Y, Wang D, Wang Z . Study on therapeutic mechanism of Neiyifang in treating endometriosis[J]. Chin J Integr Med, 2003,9(2):88-92
doi: 10.1007/BF02838554
[16] Yu CQ, Yu J, Han J, Zhou QL, Shen W . Regulatory mechanism of malignant behavior of endometriosis mediated by puerarin[J]. J Chin Integr Med, 2009,7(1):41-47
doi: 10.3736/jcim20090106
俞超芹, 俞瑾, 韩洁, 周巧玲, 沈慰 . 葛根素对子宫内膜异位症细胞恶性行为调控机制研究[J]. 中西医结合学报, 2009,7(1):41-47
doi: 10.3736/jcim20090106
[1] Deng-hai Zhang, Kun-lun Wu, Xue Zhang, Sheng-qiong Deng, Bin Peng. In silico screening of Chinese herbal medicines with the potential to directly inhibit 2019 novel coronavirus. Journal of Integrative Medicine, 2020, 18(2): 152-158.
[2] Carola Analía Torres, Cristina Marisel Pérez Zamora, María Beatriz Nuñez, Ana María Gonzalez. In vitro antioxidant, antilipoxygenase and antimicrobial activities of extracts from seven climbing plants belonging to the Bignoniaceae. Journal of Integrative Medicine, 2018, 16(4): 255-262.
[3] Shui-jie Shen, Yong-hong Zhang, Xiao-xia Gu, Shui-ju Jiang, Ling-jun Xu. Yangfei Kongliu Formula, a compound Chinese herbal medicine, combined with cisplatin, inhibits growth of lung cancer cells through transforming growth factor-β1 signaling pathway. Journal of Integrative Medicine, 2017, 15(3): 242-251.
[4] Liliana Zazueta-Beltrán, Lorena Medina-Aymerich, Nadia Estela Díaz-Triste, Aracely Evangelina Chávez-Piña, Gilberto Castañeda-Hernández, Leticia Cruz-Antonio. Evidence against the participation of a pharmacokinetic interaction in the protective effect of single-dose curcumin against gastrointestinal damage induced by indomethacin in rats. Journal of Integrative Medicine, 2017, 15(2): 151-157.
[5] Tom Fleischer, Tung-Ti Chang, Hung-Rong Yen. Post-hematopoietic stem cell transplantation in patients with hematologic disorders: Chinese herbal medicine for an unmet need. Journal of Integrative Medicine, 2016, 14(5): 322-335.
[6] JiaXia, Li-qunHe, XiaoSu. Interventional mechanisms of herbs or herbal extracts on renal interstitial fibrosis. Journal of Integrative Medicine, 2016, 14(3): 165-173.
[7] Mojtaba Heydari, Mohammad Hashem Hashempur, Mohammad Hosein Ayati, Detlev Quintern, Majid Nimrouzi, Seyed Hamdollah Mosavat. The use of Chinese herbal drugs in Islamic medicine. Journal of Integrative Medicine, 2015, 13(6): 363-367.
[8] Wei Chiang Chan Eric, Kuin Wong Siu. Phytochemistry and pharmacology of ornamental gingers, Hedychium coronarium and Alpinia purpurata: A review. Journal of Integrative Medicine, 2015, 13(6): 368-379.
[9] Xiao-ping Huang, Huang Ding, Jin-dong Lu, Ying-hong Tang, Bing-xiang Deng, Chang-qing Deng . Autophagy in cerebral ischemia and the effects of traditional Chinese medicine. Journal of Integrative Medicine, 2015, 13(5): 289-296.
[10] Swapnil P. Borse, Bhagyashree B. Kamble. Effects of Ayurvedic Rasayana botanicals on CYP3A4 isoenzyme system. Journal of Integrative Medicine, 2015, 13(3): 165-172.
[11] Elizabeth Abidemi Balogun, Sylvia Orume Malomo, Joseph Oluwatope Adebayo, Ahmed Adebayo Ishola, Ayodele Olufemi Soladoye, Lawrence Aderemi Olatunji, Olatunji Matthew Kolawole, Stephen Olubunmi Oguntoye, Abiola Samuel Babatunde, Oluwole Busayo Akinola . In vivo antimalarial activity and toxicological effects of methanolic extract of Cocos nucifera (Dwarf red variety) husk fibre. Journal of Integrative Medicine, 2014, 12(6): 504-511.
[12] Chang-qing Zhao, Yang Zhou, Jian Ping, Lie-ming Xu. Traditional Chinese medicine for treatment of liver diseases: Progress, challenges and opportunities. Journal of Integrative Medicine, 2014, 12(5): 401-408.
[13] Urmila Aswar, Mayuri Gurav, Ganesh More, Khaled Rashed, Manoj Aswar. Effect of aqueous extract of Solanum xanthocarpum Schrad. & Wendl. on postmenopausal syndrome in ovariectomized rats. Journal of Integrative Medicine, 2014, 12(5): 439-446.
[14] Benjamin Perry, Junzeng Zhang, Tarek Saleh, Yanwen Wang. Liuwei Dihuang, a traditional Chinese herbal formula, suppresses chronic inflammation and oxidative stress in obese rats. Journal of Integrative Medicine, 2014, 12(5): 447-454.
[15] Yan-bin Shi, Hui-li Li, Hai-qin Wang, Yan-biao Yang, Xiao-yun Zhang, Hui Wang, Zong-jie Zhu, Zhi-ye Zhang, Cheng-an Zhang. Simultaneous determination of five anthraquinones in a Chinese traditional preparation by RP-HPLC using an improved extraction procedure. Journal of Integrative Medicine, 2014, 12(5): 455-462.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. An explanation on "guiding principles of clinical research on mild cognitive impairment (protocol)". Journal of Chinese Integrative Medicine, 2008, 6(1): 15-21
[3] Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[4] Jun Cai, Hua Wang, Sheng Zhou, Bin Wu, Hua-rong Song, Zheng-rong Xuan. Effect of Sijunzi Decoction and enteral nutrition on T-cell subsets and nutritional status in patients with gastric cancer after operation: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(1): 37-40
[5] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[6] Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[7] Hai-feng Wei, Bai-liu Ya, Ling Zhao, Cui-fei Ye, Li Zhang, Lin Li. Evaluation of tongue manifestation of blood stasis syndrome and its relationship with blood rheological disorder in a rat model of transient brain ischemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 73-76
[8] Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[9] Li Zhou, Hong-xing Zhang, Ling-guang Liu, Wen-jun Wan. Effect of electro-acupuncture at Fenglong (GV 16) on nitric oxide and endothelin in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 89-92
[10] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013