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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (4): 373-378.doi: 10.3736/jcim20100413

• Original Experimental Research • Previous Articles     Next Articles

Effects of Ginkgo biloba extract 50 preconditioning on contents of inflammation-related cytokines in myocardium of rats with ischemia-reperfusion injury

 Yi-min Baoa, Ai-hua Liua, Zhi-xiong Zhanga, Yun Lib, Xing-yu Wangb   

  1. a Department of Physiology, School of Basic Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    b Experiment Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
  • Received:2010-01-06 Accepted:2010-02-24 Online:2010-04-20 Published:2010-04-15
  • Contact: Zhi-xiong Zhang E-mail:zixion@online.sh.cn

Objective

To investigate the effects of Ginkgo biloba extract 50 (GBE50) preconditioning on contents of inflammation-related cytokines in rats with myocardial ischemia-reperfusion.
Methods

Fifty-eight SD rats were divided into sham-operated group, untreated group, Salviae miltiorrhizae (SM) injection group and low-, medium- and high-dose GBE50 groups. After intragastric administration for 7 d, the left anterior descending (LAD) coronary artery was occluded for 30 min followed by 60-min reperfusion to induce ischemia-reperfuion injury. Myocardium histopathologic change was observed by HE staining under a light microscope; myeloperoxidase (MPO) activity in myocardium was measured by colorimetric detection; tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and IL-8 were detected by radioimmunoassay; IL-4 and IL-10 were tested by enzyme-linked immunosorbent assay (ELISA).
Results

Compared with untreated group, rats in medium-dose GBE50 group had lower inflammatory reaction and MPO activity (P<0.01). GBE50 also decreased the content of IL-6 (P<0.05, P<0.01) and increased the content of IL-4 in myocardium (P<0.05, P<0.01) as compared with the untreated group. The content of TNF-α in myocardium in the medium-dose GBE50 group was lower and IL-10 was higher than those in the untreated group, but without significant differences.
Conclusion

GBE50 can decrease the content of IL-6 and increase the content of IL-4 in myocardium after ischemia-reperfusion injury. It suggests that GBE50 can regulate the inflammatory reaction after ischemia-reperfusion injury via inhibiting inflammatory cytokines and promoting anti-inflammatory cytokines.

Key words: Myocardial ischemia-reperfusion, GBE50, Lnterleukin-6, Lnterleukin-4, Tumor necrosis factor-alpha, Interleukin-10, Rats

Figure 1

Morphological changes of myocardium after IRI observed by HE staining (Light microscopy, ×400) A: Sham-operated group; B: Untreated group; C: SM injection group; D: Medium-dose GBE50 group."

Table 1

MPO activity in myocardium in different groups after IRI ($\bar{x}$±s, U/g)"

Group n Activity of MPO
Sham-operated 8 0.75±0.16
Untreated 8 1.37±0.30**
SM injection 8 1.10±0.17**△
Low-dose GBE50 8 1.29±0.25**
Medium-dose GBE50 8 0.97±0.21*△△▲▲
High-dose GBE50 8 1.11±0.13**△

Table 2

Contents of TNF-α, IL-6 , IL-8, IL-4 and IL-10 in myocardium in different groups after IRI ($\bar{x}$±s)"

Group n TNF-α (pg/mg) IL-6 (pg/mg) IL-8 (pg/mg) IL-4 (ng/L) IL-10 (ng/L)
Sham-operated 8 13.31±2.24 0.93±0.27 5.24±1.27 71.70±7.40 143.55±12.87
Untreated 8 22.42±6.54** 1.33±0.23** 6.13±1.18 70.29±2.98 149.70±13.36
SM injection 8 18.92±5.67* 1.04±0.28 5.97±1.13 80.58±7.75*△ 157.24±23.59
Low-dose GBE50 8 20.43±4.36** 1.01±0.26 6.00±1.12 74.45±6.94 161.11±26.65
Medium-dose GBE50 8 18.08±3.24* 1.04±0.22 5.77±1.28 80.86±8.31*△ 166.91±17.76*
High-dose GBE50 8 18.69±2.00* 0.92±0.10△△ 5.13±1.63 89.89±11.38**△△▲▲□■ 167.48±15.49*
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