Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (5): 480-485.doi: 10.3736/jcim20100513

• Original Experimental Research • Previous Articles     Next Articles

Effects of electro-acupuncture on expression of obestatin in hypothalamus of rats with simple obesity

 Xian-juan Konga,b, Lei Gaoc, Hao Pengc, Xian Shia   

  1. a Department of Acupuncture and Moxibustion, General Hospital of Peopled Liberation Army, Beijing 100853, China
    b Health Section, August First Physical Culture and Sports Team of Peopled Liberation Army, Beijing 100091, China
    School of Traditional Chinese Medicine, Second Military Medical University, Shanghai 200433, China
  • Received:2010-03-31 Accepted:2010-04-21 Online:2010-05-20 Published:2010-09-15
  • Contact: Xian Shi


To investigate the effects of electro-acupuncture on obestatin in rat with simple obesity.

Obesity was induced in male SD rats with high-fat and -glucose diet for 10 weeks. Twenty-four rats with obesity were randomly divided into untreated group and electro-acupuncture group, with 12 rats in each group. Another 12 normal SD rats serving as controls were fed standard rat chow. Rats in the electro-acupuncture group were treated with electro-acupuncture on bilateral Zusanli (ST36), Tianshu (ST25) and Pishu (BL20) acupoints for fifteen days consecutively. Rats in the blank control group and untreated group were not interfered. Body weight was measured every 3 days during treatment. After treatment, obestatin in serum and hypothalamus was detected by radioimmunoassay or immunohistochemiscal method respectively. Visceral fat weight was also detected.

Body weight and visceral fat weight of rats in the untreated group were significantly higher than those in the blank control group (P<0.01). Body weight and increase of body weight in the electro-acupuncture group were significantly lower than those in the untreated group (P<0.01). Visceral fat weight in the electro-acupuncture group was significantly lower than that in the untreated group (P<0.01), but there was no significant difference as compared with the blank control group. The obestatin expression in hypothalamus was higher in the untreated group than in the blank control group (P<0.05), while it was lower in the untreated group than in the electro-acupuncture group (P<0.01).

Electro-acupuncture has an effect against obesity in rats, which may be related to up-regulating obestatin expression in hypothalamus.

Key words: Simple obesity, Electro-acupuncture, Hypothalamus, Obestatin, Rats

Table 1

Food intake in different groups (x±s, g/d)"

Group n Food intake
Day 1 Day 3 Day 6 Day 9 Day 12 Day 15
Blank control 12 32. 37±3. 71 34. 58±2.00 35. 02±2. 82 36. 68±3. 70 35.38±2.72 34.05±2. 24
Untreated 12 26. 80±2. 74* * 31. 18±3. 55“ 34. 38±2. 81 37. 75±2. 89 40. 20±3. 76* * 40. 28±2. 97 * *
Electro-acupuncture 12 27. 97±4. 93* * 25. 98±1. 74* * AA 24. 86±1. 98* * AA 28. 92±4. 11* * A 30. 07±4. 13* * AA 26.55±2. 68*

Table 2

Body weight and visceral fat weight in different groups (x±s, g)"

Group Body weight Visceral £ftt weight
Before experiment After experiment Difference
Blank control 12 612,11士42,33 673,00 土 43,36“ SO, 88 土 U, 20 25,93土2,45
Untreated 12 764, 93土30,05 ?? 801,84 土 32,13 36,92 土 12,65 ?? 38,07±S,35〃
Electro-acupuncture 12 759,45 土 29,49 ?? 733,抖土23,38 -(25, 55土 19,30) 27,29士2,63^^

Table 3

Content of obestatin in serum and expression of obestatin in hypothalamus in rats (x±s)"

Group n Obestiim content in serum (ng/L) Obestain expreasion in hypothalamus
Blank control 12 1,34 土 0,10 0,33土0,03
Untreated 12 1, 54 土0,21 ? 0,36土0,03?
Electro-acupuncture 12 1,59土0,20〃 0,40±0,04maa

Figure 2

Expression of obestatin in hypothalamus in different groups observed by immunohistochemical method (Light microscopy, ×400) A: PBS control group; B: Blank control group; C: Untreated group; D: Electro-acupuncture group."

[1] Zhang JV, Ren PG, Avsian-Kretchmer O, Luo CW, Rauch R, Klein C, Hsueh AJ . Obestatin, a peptide encoded by the ghrelin gene, opposes ghrelin’s effects on food intake[J]. Science, 2005,310(5750):996-999
doi: 10.1126/science.1117255
[2] Xu HR, Zheng X, Guo ZF . Study on circulating levels of ghrelin and obestatin in human simple obesity[J]. Xin Zang Za Zhi, 2009,21(1):95-98
徐海蓉, 郑兴, 郭志福 . 单纯性肥胖症患者外周血ghrelin、obestatin的水平变化及其临床意义[J]. 心脏杂志, 2009,21(1):95-98
[3] Wang J . The influence of high lipid diet on serum and hypothalamic ghrelin, obestatin and the ratio of rats[J]. Ti Yu Ke Xue, 2007,27(11):61-65
汪军 . 高脂饮食对大鼠血浆和下丘脑Ghrelin、Obestatin及其比值的影响[J]. 体育科学, 2007,27(11):61-65
[4] Li WH, Wang JD, Gu LM, Wang YZ . Treatment of simple obesity with electro-acupuncture and auricular acupoint pressing: a report of 177 cases[J]. J Chin Integr Med, 2004,2(6):449-450
李伟红, 汪金娣, 谷丽敏, 王勇姿 . 电针配合耳穴贴压治疗单纯性肥胖病177例临床观察[J]. 中西医结合学报, 2004,2(6):449-450
[5] Xu B, Yuan JH, Liu ZC, Chen M, Wang XJ . Effect of acupuncture on plasma peptide YY in the patient of simple obesity[J]. Zhongguo Zhen Jiu, 2005,25(12):837-840
徐斌, 袁锦虹, 刘志诚, 陈梅, 王欣君 . 针刺影响单纯肥胖病患者血浆酪酪肽水平观察[J]. 中国针灸, 2005,25(12):837-840
[6] Huang QF, Zhao SX, Huang Y . Statistical analysis of the points for acupuncture-moxibustion treatment of obesity[J]. J Chin Integr Med, 2007,5(6):695-696
黄琴峰, 赵善祥, 黄颖 . 针灸治疗肥胖病的用穴统计分析[J]. 中西医结合学报, 2007,5(6):695-696
[7] Sun Z, Zhang ZC, Liu ZC . Experiment study of diet-induced obesity animal model[J]. Zhongguo Yao Li Xue Tong Bao, 2002,18(4):466-467
孙志, 张中成, 刘志诚 . 营养性肥胖动物模型的实验研究[J]. 中国药理学通报, 2002,18(4):466-467
[8] Yu SG, Guo Y. Experimental acupuncture science[M]. Shanghai: Shanghai Scientific and Technical Publishers, 2009: 150-151
余曙光, 郭义 . 实验针灸学[M]. 上海: 上海科学技术出版社, 2009: 150-151
[9] Shanghai Medical College of Fudan University, Editorial Committee of Practical Internal Medicine, Chen HZ . Practical internal medicine(12th ed)[M]. Beijing: People’s Medical Publishing House, 2005,( 12th ed):914-917
复旦大学上海医学院, 《实用内科学》编委会, 陈灏珠 . 实用内科学(第12版)[M]. 北京: 人民卫生出版社, 2005,( 第12版):914-917
[10] Qader SS, Håkanson R, Rehfeld JF, Lundquist I, Salehi A . Proghrelin-derived peptides influence the secretion of insulin, glucagon, pancreatic polypeptide and somatostatin: a study on isolated islets from mouse and rat pancreas[J]. Regul Pept, 2008,146(1-3):230-237
doi: 10.1016/j.regpep.2007.09.017
[11] Tang SQ, Jiang QY, Zhang YL, Zhu XT, Shu G, Gao P, Feng DY, Wang XQ, Dong XY . Obestatin: its physicochemical characteristics and physiological functions[J]. Peptides, 2008,29(4):639-645
doi: 10.1016/j.peptides.2008.01.012
[12] Zhang Z, Zou DJ, Chen Y, Wang M, Wu J, Guo ZF . Obestatin inhibits proliferation and differentiation of 3T3-L1 preadipocytes[J]. Di Er Jun Yi Da Xue Xue Bao, 2007,28(9):929-932
张征, 邹大进, 陈月, 王淼, 吴捷, 郭志福 . Obestatin抑制3T3-L1前脂肪细胞的增殖与分化[J]. 第二军医大学学报, 2007,28(9):929-932
[13] Li ZY, Tang CS . Research progress in obestatin[J]. Sheng Li Ke Xue Jin Zhan, 2007,38(2):147-149
李战永, 唐朝枢 . 肥胖抑制素的研究进展[J]. 生理科学进展, 2007,38(2):147-149
[14] Nogueiras R , Tschöp M .Biomedicine. Separation of conjoined hormones yields appetite rivals[J]. Science, 2005,310(5750):985-986
doi: 10.1126/science.1121214
[1] Samwel Sylvester Msigwa, Yan Li, Xiang-lin Cheng, Fen Cao. Combining electroacupuncture and transcranial direct current stimulation as an adjuvant therapy enhances spontaneous conversation and naming in subacute vascular aphasia: A retrospective analysis. Journal of Integrative Medicine, 2022, 20(3): 244-251.
[2] Bing-rong Li, Shi-yun Shao, Long Yuan, Ru Jia, Jian Sun, Qing Ji, Hua Sui, Li-hong Zhou, Yi Zhang, Hui Liu, Qi Li, Yan Wang, Bi-meng Zhang. Effects of mild moxibustion on intestinal microbiome and NLRP3 inflammasome in rats with 5-fluorouracil-induced intestinal mucositis. Journal of Integrative Medicine, 2021, 19(2): 144-157.
[3] Sitthichai Iamsaard, Supatcharee Arun, Jaturon Burawat, Supataechasit Yannasithinon, Saranya Tongpan, Sudtida Bunsueb, Natthapol Lapyuneyong, Pannawat Choowong-in, Nareelak Tangsrisakda, Chadaporn Chaimontri, Wannisa Sukhorum. Evaluation of antioxidant capacity and reproductive toxicity of aqueous extract of Thai Mucuna pruriens seeds. Journal of Integrative Medicine, 2020, 18(3): 265-273.
[4] Xin-fang Zhang, Shui-ying Xiang, Wen-ye Geng, Wen-juan Cong, Jing Lu, Chuan-wei Jiang, Kun Wang, Zi-bing Liu . Electro-acupuncture regulates the cholinergic anti-inflammatory pathway in a rat model of chronic obstructive pulmonary disease. Journal of Integrative Medicine, 2018, 16(6): 418-426.
[5] Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[6] Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[7] Kylie Connolly, Douglas Jackson, Candice Pullen, Andrew Fenning. Alpha-adrenoceptor antagonism by Crassostrea gigas oyster extract inhibits noradrenaline-induced vascular contraction in Wistar rats. Journal of Integrative Medicine, 2015, 13(3): 194-200.
[8] Udhaya Lavinya Baskaran, Sherry Joseph Martin, Rasool Mahaboobkhan, Sabina Evan Prince. Protective role of Triphala, an Indian traditional herbal formulation, against the nephrotoxic effects of bromobenzene in Wistar albino rats. Journal of Integrative Medicine, 2015, 13(2): 115-121.
[9] Hussein O. B. Oloyede, Matthew C. Adaja, Taofeek O. Ajiboye, Musa O. Salawu. Anti-ulcerogenic activity of aqueous extract of Carica papaya seed on indomethacin-induced peptic ulcer in male albino rats. Journal of Integrative Medicine, 2015, 13(2): 105-114.
[10] Anirudha A. Lande, Shirishkumar D. Ambavade, Uma S. Swami, Prafulla P. Adkara Prashant D. Ambavade, Arun B. Waghamare. Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase. Journal of Integrative Medicine, 2015, 13(1): 25-33.
[11] Elizabeth Abidemi Balogun, Sylvia Orume Malomo, Joseph Oluwatope Adebayo, Ahmed Adebayo Ishola, Ayodele Olufemi Soladoye, Lawrence Aderemi Olatunji, Olatunji Matthew Kolawole, Stephen Olubunmi Oguntoye, Abiola Samuel Babatunde, Oluwole Busayo Akinola . In vivo antimalarial activity and toxicological effects of methanolic extract of Cocos nucifera (Dwarf red variety) husk fibre. Journal of Integrative Medicine, 2014, 12(6): 504-511.
[12] Urmila Aswar, Mayuri Gurav, Ganesh More, Khaled Rashed, Manoj Aswar. Effect of aqueous extract of Solanum xanthocarpum Schrad. & Wendl. on postmenopausal syndrome in ovariectomized rats. Journal of Integrative Medicine, 2014, 12(5): 439-446.
[13] Xin-fang Zhang, Ji Zhu, Wen-ye Geng, Shu-jun Zhao, Chuan-wei Jiang, Sheng-rong Cai, Miao Cheng, Chuan-yun Zhou, Zi-bing Liu. Electroacupuncture at Feishu (BL13) and Zusanli (ST36) down-regulates the expression of orexins and their receptors in rats with chronic obstructive pulmonary disease. Journal of Integrative Medicine, 2014, 12(5): 417-424.
[14] Benjamin Perry, Junzeng Zhang, Tarek Saleh, Yanwen Wang. Liuwei Dihuang, a traditional Chinese herbal formula, suppresses chronic inflammation and oxidative stress in obese rats. Journal of Integrative Medicine, 2014, 12(5): 447-454.
[15] Upendarrao Golla, Praveen Kumar Gajam, Solomon Sunder Bhimathati. Evaluation of diuretic and laxative activity of hydro-alcoholic extract of Desmostachya bipinnata (L.) Stapf in rats. Journal of Integrative Medicine, 2014, 12(4): 372-378.
Full text



[1] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[2] Hai-feng Wei, Bai-liu Ya, Ling Zhao, Cui-fei Ye, Li Zhang, Lin Li. Evaluation of tongue manifestation of blood stasis syndrome and its relationship with blood rheological disorder in a rat model of transient brain ischemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 73-76
[3] Xi Lin, Jian-ping Liu. Herbal medicines for viral myocarditis. Journal of Chinese Integrative Medicine, 2008, 6(1): 76
[4] Xi Lin, Jian-ping Liu. Tai chi for treating rheumatoid arthritis. Journal of Chinese Integrative Medicine, 2008, 6(1): 82
[5] Liang-ping Hu, Hui Gao. Discrimination of errors in statistical analysis of medical papers published in the first issue of 2006 in Journal of Chinese Integrative Medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 98-106
[6] Yan-bo Zhu , Qi Wang, Cheng-yu Wu, Guo-ming Pang, Jian-xiong Zhao, Shi-lin Shen, Zhong-yuan Xia , Xue Yan . Logistic regression analysis on relationships between traditional Chinese medicine constitutional types and overweight or obesity. Journal of Chinese Integrative Medicine, 2010, 8(11): 1023-1035
[7] Wei Xu, Meng Shi, Jian-gang Liu, Cheng-long Wang . Collagen protein expressions in ischemic myocardium of rats with acute myocardial infarction and effects of qi-tonifying, yin-tonifying and blood-activating herbs and detoxifying and blood-activating herbs. Journal of Chinese Integrative Medicine, 2010, 8(11): 1041-1047
[8] Tao Wang , Feng Qin. Effects of Chinese herbal medicine Xiaoyao Powder on monoamine neurotransmitters in hippocampus of rats with postpartum depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1075-1079
[9] Ying Xu , Chang-chun Zeng , Xiu-yu Cai , Rong-ping Guo , Guang Nie , Ying Jin. Chromaticity and optical spectrum colorimetry of the tongue color in different syndromes of primary hepatic carcinoma. Journal of Chinese Integrative Medicine, 2012, 10(11): 1263-1271
[10] Xiang-ying Mao , Qin Bian , Zi-yin Shen. Analysis of the osteogenetic effects exerted on mesenchymal stem cell strain C3H10T1/2 by icariin via MAPK signaling pathway in vitro. Journal of Chinese Integrative Medicine, 2012, 10(11): 1272-1278