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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (10): 958-962.doi: 10.3736/jcim20091009

• Original Experimental Research • Previous Articles     Next Articles

Tongxie Yaofang inhibits the contraction of colonic smooth muscle isolated from rats through a mechanism related to calcium mobilization

Jian-ye Yuana,b,Jian-qun Xieb,Da-zheng Wua,Yu Zhengb,Xiang-xue Panc,Xiao-yan Feib,Hai-zhen Xub   

  1. a Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    b Institute of Digestive Diseases, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, China
    c Department of Gastroenterology, Shanghai Hospital of Traditional Chinese Medicine, Shanghai 200071, China
  • Received:2009-05-31 Accepted:2009-08-20 Online:2009-10-20 Published:2009-10-15
  • Contact: Jian-qun Xie E-mail:xiejq@gmail.com

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Objective

To study the relationship between the inhibitory effects of Tongxie Yaofang, a compound traditional Chinese herbal medicine, on the contraction of the colonic smooth muscle isolated from rats and calcium mobilization.
Methods

By measuring the tension of the isolated colonic smooth muscle strips, the inhibitory effects of Tongxie Yaofang on the contraction induced by acetylcholine (ACh), KCl and exhausting Ca2+ of internal calcium store were assessed respectively.
Results

Tongxie Yaofang could concentration-dependently inhibit the contraction of isolated rat colonic smooth muscle strips induced by KCl and exhausting the Ca 2+of internal calcium store. Tongxie Yaofang could also inhibit the tension of the second contractile phase induced by ACh (P<0.01, vs control), but had no influence on the first contractile phase. Conclusion: Tongxie Yaofang can inhibit the contraction of isolated rat colonic smooth muscle strips mainly by preventing the influx of extracellular Ca 2+, which may be associated with blocking voltage-dependent channel, store-operated channel and receptor-operated channel, but not by preventing the release of internal Ca 2+from calcium store.

Key words: Tongxie Yaofang, Colon, Smooth muscle, Calcium mobilization, Rats

Figure 1

Inhibitory effects of Tongxie Yaofangon contraction of muscle strips induced by KCl Data were represented as $\bar{x}$±s. n=6. **P<0.01, vs control."

Figure 2

Inhibitory effects of Tongxie Yaofang on contraction of muscle strips induced by exhausting Ca2+ of internal calcium store Data were represented as $\bar{x}$±s. n=6. **P<0.01, vs control."

Table 1

Effects of Tongxie Yaofang on two phases of the contraction induced by Ach ($\bar{x}$±s, %)"

Group n Percent tension of the first contractile phase Percent tension of the second contractile phase
Krebs 6 48.81±5.85 111.28±5.74
300 μg/mL Tongxie Yaofang 6 50.49±7.15 101.76±4.31
1 000 μg/mL Tongxie Yaofang 6 56.41±6.18 91.60±3.63
3 000 μg/mL Tongxie Yaofang 6 47.12±4.97 61.47±7.38**
[1] Chu GW . Textual research on Tongxie Yaofang[J]. Zhong Yi Yao Xue Kan, 2006,24(11):2036-2037
楚更五 . 痛泻要方考释[J]. 中医药学刊, 2006,24(11):2036-2037
[2] Fang P . Clinical effect of Jiaweitongxie prescription in treating irritable bowel syndrome[J]. Zhejiang Zhong Xi Yi Jie He Za Zhi, 2008,18(8):609-670
doi: 10.3969/j.issn.1005-4561.2008.08.004
方萍 . 加味痛泻要方治疗肠易激综合征疗效观察[J]. 浙江中西医结合杂志, 2008,18(8):609-670
doi: 10.3969/j.issn.1005-4561.2008.08.004
[3] Wu YN, Huang DH . Clinical observation of Tongxie Yaofang in treatment of patients with diarrhea-type irritable bowel syndrome[J]. Liaoning Zhong Yi Yao Da Xue Xue Bao, 2008,10(6):94-95
doi: 10.3969/j.issn.1673-842X.2008.06.054
吴耀南, 黄墩煌 . 痛泻要方加味治疗腹泻型肠易激综合征临床观察[J]. 辽宁中医药大学学报, 2008,10(6):94-95
doi: 10.3969/j.issn.1673-842X.2008.06.054
[4] Wang BB, Chu GW, Zheng M . Effect of Tongxie Yaofang and acetylcholine on the smooth muscle isolated from the small intestine in rabbit[J]. Zhejiang Zhong Yi Za Zhi, 2007,42(4):235-237
doi: 10.3969/j.issn.0411-8421.2007.04.038
王蓓蓓, 楚更五, 郑梅 . 痛泻要方与乙酰胆碱对家兔离体小肠平滑肌作用的研究[J]. 浙江中医杂志, 2007,42(4):235-237
doi: 10.3969/j.issn.0411-8421.2007.04.038
[5] Chu GW, Zhang JY, Rao GX, Li P, Yang Y, Liang L, Nie J . Effects of the water extracts and its remnant components of Tongxieyaofang decoction on the motility of intestinal muscle in rats[J]. Zhongguo Zhong Xi Yi Jie He Xiao Hua Za Zhi, 2007,15(3):153-155
doi: 10.3969/j.issn.1671-038X.2007.03.005
楚更五, 张建英, 饶高雄, 李平, 杨云, 梁玲, 聂坚 . 痛泻要方水提取物及其萃取成分对大鼠体外肠肌的作用[J]. 中国中西医结合消化杂志, 2007,15(3):153-155
doi: 10.3969/j.issn.1671-038X.2007.03.005
[6] Myers BS, Martin JS, Dempsey DT, Parkman HP, Thomas RM, Ryan JP . Acute experimental colitis decreases colonic circular smooth muscle contractility in rats[J]. Am J Physiol, 1997,273(4 Pt 1):G928-G936
doi: 10.1152/ajpcell.1997.273.3.C928
[7] Dai Y, Liu JX, Li JX, Xu YF . Effect of pinaverium bromide on stress-induced colonic smooth muscle contractility disorder in rats[J]. World J Gastroenterol, 2003,9(3):557-561
doi: 10.3748/wjg.v9.i3.557
[8] Ke DP, Zhou H, Li ZW, Kong DH, Ma R . Capacitative Ca2+ entry mediates contraction in rat distal colon smooth muscle[J]. Zhongguo Yao Li Xue Tong Bao, 2006,22(3):344-348
柯道平, 周华, 李忠稳, 孔德虎, 马嵘 . 库容性Ca2+内流介导大鼠远端结肠平滑肌收缩[J]. 中国药理学通报, 2006,22(3):344-348
[9] Yang BF. Pharmacology on ion channel[M]. Beijing: People's Medical Publishing House,2005:155, 161, 172
杨宝峰 . 离子通道药理学[M]. 北京: 人民卫生出版社,2005:155, 161, 172
[10] Kinoshita K, Sato K, Hori M, Ozaki H, Karaki H . Decrease in activity of smooth muscle L-type Ca2+ channels and its reversal by NF-kappaB inhibitors in Crohn's colitis model[J]. Am J Physiol Gastrointest Liver Physiol, 2003,285(3):G483-G493
doi: 10.1152/ajpgi.00038.2003
[11] McCarron JG, Craig JW, Bradley KN, Muir TC . Agonist-induced phasic and tonic responses in smooth muscle are mediated by InsP3[J]. J Cell Sci, 2002,115(Pt 10):2207-2218
[12] Yu ZP, Li XF, Li WJ, He XS, Lin Q . Effects and mechanism of extracting G2 from Gastrodia Elata Blume on relaxation of isolated ileum smooth muscle[J]. Yunnan Zhong Yi Xue Yuan Xue Bao, 2005,28(3):15-18
淤泽溥, 李秀芳, 李文军, 何晓山, 林青 . 天麻提取物对回肠平滑肌的舒张作用及其机制研究[J]. 云南中医学院学报, 2005,28(3):15-18
[13] Zhang WW, Li Y, Wang XQ, Tian F, Cao H, Wang MW, Sun QS . Effects of magnolol and honokiol derived from traditional Chinese herbal remedies on gastrointestinal movement[J]. World J Gastroenterol, 2005,11(28):4414-4418
doi: 10.3748/wjg.v11.i28.4414
[14] Karaki H, Ozaki H, Hori M, Mitsui-Saito M, Amano K, Harada K, Miyamoto S, Nakazawa H, Won KJ, Sato K . Calcium movements, distribution and functions in smooth muscle[J]. Pharmacol Rev, 1997,49(2):157-230
[15] Yuan JY, Xie JQ . Inhibitory effect of extract from Shuganyin on contractility of rats' colonic smooth muscles[J]. Shanghai Zhong Yi Yao Da Xue Xue Bao, 2007,21(1):50-53
doi: 10.3969/j.issn.1008-861X.2007.01.018
袁建业, 谢建群 . 疏肝饮提取物对大鼠结肠平滑肌收缩的抑制作用[J]. 上海中医药大学学报, 2007,21(1):50-53
doi: 10.3969/j.issn.1008-861X.2007.01.018
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