Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (1): 65-68.doi: 10.3736/jcim20070113

• Original Experimental Research • Previous Articles     Next Articles

Effects of inactivated rabbit serum containing compound realgar and natural indigo tablet on cell line NB4

Nan-nan Chen1, Shi-lin Huang2 , Yang Xiang2, De-jie Zhang2, Ai-xia Guo2, Ai-ping Chen2   

  1. 1. Graduate School of General Hospital of People’s Liberation Army,Beijing 100853, China;
    2. Department of Traditional Chinese Medicine and Hematology, Dalian 210th Hospital of People’s Liberation Army, Dalian, Liaoning Province 116021, China;
  • Online:2007-01-31 Published:2007-01-21

Objective: To explore the effects of inactivated rabbit serum containing compound realgar and natural indigo tablet (CRNIT) on cell line NB4. 

Methods: The experimental rabbits were taken as the provider of the animal serum, and the serum was inactivated before the experiment. The serum was divided into two groups based on whether the rabbits were given CRNIT. The concentration of arsenic in the rabbit's serum was detected by AFS-230a double path atom fluorescence photometer. The inhibition rates and apoptosis rates were regarded as the observational indexes.

Results: The concentration of arsenic in the inactivated rabbit serum containing and not containing the drug were (0.010 0±0.001 0) mg/L and (0.110 0±0.006 4) mg/L respectively, and the difference had statistical significance (P<0.01). The two groups of serum all had inhibitory effect on the growth of NB4 cells depending on the drug concentration and effect time. And there were significant differences among the groups. The two groups of serum all induced the apoptosis of NB4 with positive relations with the concentration and effect time. And there were significant differences among the groups.

Conclusion: The rabbit serum containing CRNIT can obviously restrain the growth of NB4 cells and the inhibitory effect depends on the concentration and effect time. And the rabbit serum containing CRNIT can also induce the apoptosis of NB4 cell line and the apoptosis rates depend on the concentration and effect time.

Key words: rabbits, apoptosis, serum, leukemia, Chinese herbal drugs

CLC Number: 

  • R693

Table 1

Inhibition rates of NB4 cells after 24- and 48-hour treatment of two groups of rabbit serum ($\bar{x}±S$,%)"

Apoptosis rates of NB4 cells after 24- and 48-hour treatment of two groups of rabbit serum ($\bar{x}±S$,%)"

[1] 黄世林, 郭爱霞, 向阳, 等.复方青黛片为主治疗急性早幼粒细胞白血病的临床研究[J].中华血液学杂志,1995,16(1):26-28
Huang SL, Guo AX, Xiang Y, et al.Clinical effects of compound realgar natural indigo tablets on acute promyelocytic leukemia[J].Zhonghua Xue Ye Xue Za Zhi,1995,16(1):26-28
[2] 白月辉, 黄世林.雄黄对NB4及HL-60细胞的促凋亡作用[J].中华血液学杂志,1998,19(9):477-480
Bai YH, Huang SL.Studies on red orpiment induction of NB4 and HL-60 cell apoptosis[J].Zhonghua Xue Ye Xue Za Zhi,1998,19(9):477-480
[3] 张晨, 黄世林, 卢步峰, 等.雄黄诱导K562细胞凋亡的研究[J].中国中医基础医学杂志,1999,5(3):30-31
Zhang C, Huang SL, Lu BF, et al.Study on the apoptosis of K562 cells induced by realgar[J].Zhongguo Zhong Yi Ji Chu Yi Xue Za Zhi,1999,5(3):30-31
[4] 张晨, 黄世林.雄黄抗白血病细胞多药耐药及其凋亡诱导关系的研究[J].中国中医基础医学杂志,1999,5(12):40-42
Zhang C, Huang SL.Study of realgar on the multidrug resistance of leukemic cells and its relationship with apoptosis induction[J].Zhongguo Zhong Yi Ji Chu Yi Xue Za Zhi,1999,5(12):40-42
[5] Iwama H, Amagaya S, Ogihara Y.Effect of Shosaikoto, a Japanese and Chinese traditional herbal medicinal mixture, on the mitogenic activity of lipopolysaccharide: A new pharmacology testing method[J].J Ethnopharmacol,1987,21(1):45-53
[6] 崔晓兰, 贺玉琢, 高英杰, 等.中药药理学研究的新思路——中药血清药理学[J].中国中医药科技,1997,4(4):239, 250
Cui XL, He YZ, Gao YJ, et al.New thinking about the research of pharmacology of traditional Chinese drugs: serum pharmacology of traditional Chinese drugs[J].Zhongguo Zhong Yi Yao Ke Ji,1997,4(4):239, 250
[7] 蒙一纯, 丁霞, 贲长恩, 等.中药血清药理学应用研究展望[J].北京中医药大学学报,1999,22(4):42-43
Ming YC, Ding X, Pen CE, et al.Prospects of research on application of serum pharmacology of traditional Chinese drugs[J].Beijing Zhong Yi Yao Da Xue Xue Bao,1999,22(4):42-43
[8] 黄海茵, 郭映华, 于尔辛.体外细胞培养应用于中药复方研究的进展[J].中国中西医结合杂志,2000,20(5):394-396
Huang HY, Guo YH, Yu EX.Progress on application of cell incubation on research of composite Chinese recipe[J].Zhongguo Zhong Xi Yi Jie He Za Zhi,2000,20(5):394-396
[9] 韩克起, 凌昌全.中药体外抗肿瘤效应血清药理学研究现状与前景[J].中国中西医结合杂志,2003,23(9):717-719
Han KQ, Ling CQ.Current state and prospects of sero-pharmacological study on extracorporeal anti-tumor effects of Chinese herbal medicines[J].Zhongguo Zhong Xi Yi Jie He Za Zhi,2003,23(9):717-719
[10] 王晓波, 龚荣刚, 姚文, 等.复方黄黛片在健康志愿者体内的药代动力学研究[J].解放军药学学报,2002,18(5):265-267
Wang XB, Gong RG, Yao W, et al.Study on pharmacokinetics of the Compound Realgar Natural Indigo Tablets in healthy volunteers[J].Jie Fang Jun Yao Xue Xue Bao,2002,18(5):265-267
[11] 王晓波, 龚荣刚, 张治然, 等.纳米级雄黄粉体药代动力学研究[J].解放军药学学报,2002,18(6):324-326
Wang XB, Gong RG, Zhang ZR, et al.Study on pharmacokinetics of nanoparticle realgar powders in rabbit[J].Jie Fang Jun Yao Xue Xue Bao,2002,18(6):324-326
[12] 张良, 徐立, 袁冬萍, 等.中药血清药理学方法的研究进展[J].南京中医药大学学报 (自然科学版),2002,18(4):254-256
Zhang L, Xu L, Yuan DP, et al.Progress in methods of serum pharmacology of traditional Chinese drug[J].Nanjing Zhong Yi Yao Da Xue Xue Bao (Zi Ran Ke Xue Ban),2002,18(4):254-256
[1] Maged Mohamed Maher Abou-Hashem, Dina Mohamed Abo-elmatty, Noha Mostafa Mesbah, Ahmed Mohamed Abd EL-Mawgoud. Induction of sub-G0 arrest and apoptosis by seed extract of Moringa peregrina (Forssk.) Fiori in cervical and prostate cancer cell lines. Journal of Integrative Medicine, 2019, 17(6): 410-422.
[2] Supakanya Kumkarnjana, Rutt Suttisri, Ubonthip Nimmannit, Apirada Sucontphunt, Mattaka Khongkow, Thongchai Koobkokkruad, Nontima Vardhanabhuti. Flavonoids kaempferide and 4,2′-dihydroxy-4′,5′,6′-trimethoxychalcone inhibit mitotic clonal expansion and induce apoptosis during the early phase of adipogenesis in 3T3-L1 cells. Journal of Integrative Medicine, 2019, 17(4): 288-295.
[3] Shriniwas S. Basaiyye, Sanjay Kashyap, Kannan Krishnamurthi, Saravanadevi Sivanesan. Induction of apoptosis in leukemic cells by the alkaloid extract of garden cress (Lepidium sativum L.). Journal of Integrative Medicine, 2019, 17(3): 221-228.
[4] Carlos R. Oliveira, Daniel G. Spindola, Daniel M. Garcia, Adolfo Erustes, Alexandre Bechara, Caroline Palmeira-dos-Santos, Soraya S. Smaili, Gustavo J.S. Pereira, André Hinsberger, Ezequiel P. Viriato, Maria Cristina Marcucci, Alexandra C.H.F. Sawaya, Samantha L. Tomaz, Elaine G. Rodrigues, Claudia Bincoletto. Medicinal properties of Angelica archangelica root extract: Cytotoxicity in breast cancer cells and its protective effects against in vivo tumor development. Journal of Integrative Medicine, 2019, 17(2): 132-140.
[5] Bruno José Martins Da Silva, Sandro Wilson Gomes Pereira, Ana Paula Drummond Rodrigues, José Luiz Martins Do Nascimento, Edilene Oliveira Silva. In vitro antileishmanial effects of Physalis angulata root extract on Leishmania infantum. Journal of Integrative Medicine, 2018, 16(6): 404-410.
[6] Eric Wei Chiang Chan, Siu Kuin Wong, Hung Tuck Chan . Casticin from Vitex species: A short review on its anticancer and anti-inflammatory properties. Journal of Integrative Medicine, 2018, 16(3): 147-152.
[7] Jesmin Mondal, Asmita Samadder, Anisur Rahman Khuda-Bukhsh. Psorinum 6× triggers apoptosis signals in human lung cancer cells. Journal of Integrative Medicine, 2016, 14(2): 143-153.
[8] Qing-qing Zang, Lu Zhang, Ning Gao, Cheng Huang. Ophiopogonin D inhibits cell proliferation, causes cell cycle arrest at G2/M, and induces apoptosis in human breast carcinoma MCF-7 cells. Journal of Integrative Medicine, 2016, 14(1): 51-59.
[9] Santu Kumar Saha, Sourav Roy, Anisur Rahman Khuda-Bukhsh. Ultra-highly diluted plant extracts of Hydrastis canadensis and Marsdenia condurango induce epigenetic modifications and alter gene expression profiles in HeLa cells in vitro. Journal of Integrative Medicine, 2015, 13(6): 400-411.
[10] Samrat Ghosh, Sourav Sikdar, Avinaba Mukherjee, Anisur Rahman Khuda-Bukhsh. Evaluation of chemopreventive potentials of ethanolic extract of Ruta graveolens against A375 skin melanoma cells in vitro and induced skin cancer in mice in vivo. Journal of Integrative Medicine, 2015, 13(1): 34-44.
[11] Samrat Ghosh, Kausik Bishayee, Anisur Rahman Khuda-Bukhsh​. Oleanolic acid isolated from ethanolic extract of Phytolacca decandra induces apoptosis in A375 skin melanoma cells: Drug-DNA interaction and signaling cascade. Journal of Integrative Medicine, 2014, 12(2): 102-114.
[12] Paul Avijit, Das Sreemanti, Das Jayeeta, Samadder Asmita, Bishayee Kausik, Sadhukhan Ratan, Anisur Rahman Khuda-Bukhsh. Diarylheptanoid-myricanone isolated from ethanolic extract of Myrica cerifera shows anticancer effects on HeLa and PC3 cell lines: Signalling pathway and drug-DNA interaction. Journal of Chinese Integrative Medicine, 2013, 11(6): 405-415.
[13] Farha A. Kabeer, Geetha B. Sreedevi, Mangalam S. Nair, Dhanya S. Rajalekshmi, Latha P. Gopalakrishnan, Sujathan Kunjuraman, Remani Prathapan. Antineoplastic effects of deoxyelephantopin, a sesquiterpene lactone from Elephantopus scaber, on lung adenocarcinoma (A549) cells. Journal of Chinese Integrative Medicine, 2013, 11(4): 269-277.
[14] Samrat Ghosh, Kausik Bishayee, Avijit Paul, Avinaba Mukherjee, Sourav Sikdar, Debrup Chakraborty, Naoual Boujedaini, Anisur Rahman Khuda-Bukhsh. Homeopathic mother tincture of Phytolacca decandra induces apoptosis in skin melanoma cells by activating caspase-mediated signaling via reactive oxygen species elevation. Journal of Chinese Integrative Medicine, 2013, 11(2): 116-124.
[15] Avijit Paul, Kausik Bishayee, Samrat Ghosh, Avinaba Mukherjee, Sourav Sikdar, Debrup Chakraborty, Naoual Boujedaini, Anisur Rahman Khuda-Bukhsh. Chelidonine isolated from ethanolic extract of Chelidonium majus promotes apoptosis in HeLa cells through p38-p53 and PI3K/AKT signalling pathways. Journal of Chinese Integrative Medicine, 2012, 10(9): 1025-1038.
Full text



[1] Yi-ting He, Qing-lin Zha, Jian-ping Yu, Yong Tan, Cheng Lu, Ai-ping Lv. Principal factor analysis of symptoms of rheumatoid arthritis and their correlations with efficacy of traditional Chinese medicine and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 32-36
[2] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[3] Xi Lin, Jian-ping Liu. Tai chi for treating rheumatoid arthritis. Journal of Chinese Integrative Medicine, 2008, 6(1): 82
[4] Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[5] Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[6] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[7] Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[8] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[9] Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239
[10] Nian-hong Wang , Jun-tao Yan , Wu-quan Sun , Yong-shan Hu , Jun Xia , Li-cheng Wei , Jie Jia , Gui-lin Ouyang , Yong He , Yan-ming Guo , Jie Xu . Effects of early application of Tuina treatment on quadriceps surface myoelectricity in patients after total knee arthroplasty: a randomized controlled trial. Journal of Chinese Integrative Medicine, 2012, 10(11): 1247-1253