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Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (3): 328-332.doi: 10.3736/jcim20110315

• Original Experimental Research • Previous Articles     Next Articles

Protective effects of astragaloside against ultraviolet A-induced photoaging in human fibroblasts

Xin Liu1, Wei Min2()   

  1. 1. Department of Dermatology, Jiangsu Province Official Hospital, Nanjing 210024, Jiangsu Province, China
    2. Department of Dermatology, the First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China
  • Received:2010-08-26 Accepted:2010-09-13 Online:2011-03-20 Published:2011-03-15
  • Contact: Min Wei

Objective: In this study, we aim to investigate the protective effects of astragaloside on ultraviolet A (UVA)-induced photoaging in human fibroblasts and its possible mechanisms.
Methods: Subconfluent fibroblasts were cultured and divided into normal control group, astragaloside group, UVA irradiation group and UVA plus astragaloside group. The cells were shammed or irradiated with 10 J/cm2 of UVA irradiation and treated with 20 μg/mL astragaloside. The aging condition was determined by histochemical staining of senescence-associated β-galactosidase (SA-β-gal). Concentration of transforming growth factor-β1 (TGF-β1) in the supernatant was determined by enzyme-linked immunosorbent assay, and mRNA levels of matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1) were measured by real-time polymerase chain reaction
Results: UVA irradiation raised the proportion of SA-β-gal-positive cells in comparison with the normal control group (P<0.05). Astragaloside treatment was shown to decrease the level of SA-β-gal compared with the UVA group. With UVA irradiation, the concentration of TGF-β1 in the supernatant decreased, and astragaloside treatment recovered the content of TGF-β1 compared with the UVA irradiation alone (P<0.05). UVA irradiation also up-regulated the mRNA levels of MMP-1 and TIMP-1 (P<0.05). Astragaloside decreased the mRNA level of MMP-1 compared with the UVA irradiation alone, while the TIMP-1 expression increased (P<0.05).
Conclusion: Astragaloside can protect the skin from UVA irradiation. The mechanism involved may be related with TGF secretion and decrease of collagen degradation.

Key words: ultraviolet rays, fibroblast, astragaloside, transforming growth factor-beta 1, matrix metalloproteinase-1, tissue inhibitor of metalloproteinase-1

Figure 1

Effects of astragaloside on UVA-induced photoaging feature of fibroblasts tested by cytochemical staining of SA-β-gal (Light microscopy, ×200) Cells were incubated with or without astragaloside (20 μg/mL), while receiving sham irradiation or irradiated with UVA (10 J/cm2) for 2 weeks. The expression of SA-β-gal, such as blue nuclear staining was observed under a light microscope (×200 magnification). UVA: ultraviolet A; SA-β-gal: senescence-associated β-galactosidase."




[1] Zhang DM, Qin Y, Niu FL, Zhu LQ, Wang SR . Effects of astragalosides on type Ⅰ collagen synjournal of rat cardiac fibroblasts[J]. Zhonghua Zhong Yi Yao Xue Kan, 2009,27(5):967-969
张冬梅, 秦英, 牛福玲, 朱陵群, 王硕仁 . 黄芪甲苷对心肌成纤维细胞Ⅰ型胶原合成的影响[J]. 中华中医药学刊, 2009,27(5):967-969
[2] Li WZ, Li WP, Yin YY, Gong HL, Wu GC, Zhu FF . Protective effects of AST and ASI on memory impairment and its mechanism in senescent rats treated by GC[J]. Zhongguo Zhong Yao Za Zhi, 2009,34(2):199-203
李维祖, 李卫平, 尹艳艳, 公惠玲, 吴国翠, 朱芬芳 . 黄芪总苷及黄芪甲苷对糖皮质激素诱导老前期大鼠记忆损伤的保护作用及机制[J]. 中国中药杂志, 2009,34(2):199-203
[3] Wang ML, Wu XH, Zhou JF, Wang R, Wang BT, Yang ZL . Effects of astragaloside on oxidative damage of human fibroblasts induced by ultraviolet-A irradiation in vitro[J]. Zhongguo Zhong Xi Yi Jie He Pi Fu Xing Bing Xue Za Zhi, 2009,8(5):268-270
doi: 10.3969/j.issn.1672-0709.2009.05.002
王玫玲, 吴秀红, 周静芬, 王蓉, 王宝涛, 杨子良 . 黄芪甲苷抑制长波紫外线引起人成纤维细胞氧化损伤的研究[J]. 中国中西医结合皮肤性病学杂志, 2009,8(5):268-270
doi: 10.3969/j.issn.1672-0709.2009.05.002
[4] Helfrich YR, Sachs DL, Voorhees JJ . Overview of skin aging and photoaging [J].Dermatol Nurs , 2008, 20(3):177-183; quiz 184
doi: 10.1016/S0190-9622(89)70227-9 pmid: 18649702
[5] Krutmann J . The role of UVA rays in skin aging[J]. Eur J Dermatol, 2001,11(2):170-171
doi: 10.1046/j.1524-4725.2001.00321-2.x pmid: 11275823
[6] Katiyar SK . UV-induced immune suppression and photocarcinogenesis: chemoprevention by dietary botanical agents[J]. Cancer Lett, 2007,255(1):1-11
doi: 10.1016/j.canlet.2007.02.010
[7] Li WZ, Li WP, Yin YY . Effects of AST and ASI on metabolism of free radical in senescent rats treated by HC[J]. Zhongguo Zhong Yao Za Zhi, 2007,32(23):2539-2542
doi: 10.3321/j.issn:1001-5302.2007.23.023
李维祖, 李卫平, 尹艳艳 . 黄芪总苷及黄芪甲苷对糖皮质激素诱导衰老大鼠氧自由基代谢的影响[J]. 中国中药杂志, 2007,32(23):2539-2542
doi: 10.3321/j.issn:1001-5302.2007.23.023
[8] Choi MS, Yoo MS, Son DJ, Jung HY, Lee SH, Jung JK, Lee BC, Yun YP, Pyo HB, Hong JT . Increase of collagen synjournal by obovatol through stimulation of the TGF-β signaling and inhibition of matrix metalloproteinase in UVB-irradiated human fibroblast[J]. J Dermatol Sci, 2007,46(2):127-137
doi: 10.1016/j.jdermsci.2007.02.001
[9] Dong KK, Damaghi N, Picart SD, Markova NG, Obayashi K, Okano Y, Masaki H, Grether-Beck S, Krutmann J, Smiles KA, Yarosh DB . UV-induced DNA damage initiates release of MMP-1 in human skin[J]. Exp Dermatol, 2008,17(12):1037-1044
doi: 10.1111/j.1600-0625.2008.00747.x pmid: 18459971
[10] Domeij H, Modéer T, Quezada HC, Yucel-Lindberg T . Cell expression of MMP-1 and TIMP-1 in co-cultures of human gingival fibroblasts and monocytes: the involvement of ICAM-1[J]. Biochem Biophys Res Commun, 2005,338(4):1825-1833
doi: 10.1016/j.bbrc.2005.10.137
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