川芎嗪对血管紧张素Ⅱ诱导的大鼠心肌成纤维细胞增殖及Ⅰ型胶原合成的影响

doi:10.3736/jcim20090307

Abstract

Objective

To observe the effects of tetramethylpyrazine (TMP) on the proliferation and type Ⅰ collagen synthesis of rat cardiac fibroblasts (CFBs) induced by angiotensin Ⅱ (Ang Ⅱ), and to explore the mechanism of TMP in treating myocardial fibrosis.
Methods

CFBs were isolated from neonatal rats, and the fourth-passage CFBs were used in the entire test and were stimulated by 0.1 μmol/L Ang Ⅱ in vivo. The CFB proliferation was measured by methyl thiazolyl tetrazolium (MTT) assay. Type Ⅰ collagen in the cell culture supernatant was measured by enzyme-linked immunosorbent assay. The expression of mRNA of type Ⅰ collagen was semi-quantitatively measured by reverse transcription-polymerase chain reaction (RT-PCR).
Results

(1) In MTT assay, the optical density of CFBs cultured with 0.1 μmol/L Ang Ⅱ was higher than that of the blank control cultured with 2% fetal bovine serum-Dulbecco’s modified Eagle’s medium (FBS-DMEM). The difference was statistically significant (P<0.05). Both optical densities of CFBs cultured with 0.1 μmol/L Ang Ⅱ plus 800 μg/mL TMP and 0.1 μmol/L Ang Ⅱ plus 600 μg/mL TMP were lower than that of CFBs cultured with 0.1 μmol/L Ang Ⅱ, but only the difference between 0.1 μmol/L AngⅡ plus 800 μg/mL TMP group and 0.1 μmol/L Ang Ⅱ group was significant (P<0.05). (2) The content of type Ⅰ collagen secreted by CFBs cultured with 0.1 μmol/L Ang Ⅱ was higher than that with 2% FBS-DMEM (P<0.01). The content of type Ⅰ collagen secreted by CFBs cultured with 0.1 μmol/L Ang Ⅱ plus 800 μg/mL TMP was lower than that with 0.1 μmol/L Ang Ⅱ (P<0.05). (3) The level of type Ⅰ collagen mRNA in 0.1 μmol/L Ang Ⅱ group was higher than that in blank control group, and lower than that in 0.1 μmol/L Ang Ⅱ plus 800 μg/mL TMP group. Both the differences between 0.1 μmol/L Ang Ⅱ group and the blank control group and between 0.1 μmol/L Ang Ⅱ group and 0.1 μmol/L Ang Ⅱ plus 800 μg/mL TMP group were statistically significant (P<0.05).
Conclusion

TMP can not only inhibit the proliferation of CFBs, but also decrease the secretion and the mRNA expression level of collagen Ⅰ in cultured CFBs of rat which are increased by Ang Ⅱ.

Key words: Tetramethylpyrazine, Cardiac fibroblasts, Type I collagen, Angiotensin H, Rats

Dong-mei Zhang , Ying Qin , Xi-ying Lu , Ai-ming Wu , Ling-qun Zhu , Shuo-ren Wang , Liang-duo Jiang . Effects of tetramethylpyrazine on angiotensin Ⅱ-induced proliferation and type Ⅰ collagen synthesis of rat cardiac fibroblasts. Journal of Chinese Integrative Medicine, 2009, 7(3): 232-236.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20090307

http://www.jcimjournal.com/EN/Y2009/V7/I3/232

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References 12

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Group	n	Optical density
Blank control	6	0.109±0.014
0.1 μmol/L Ang Ⅱ	6	0.133±0.011*
0.1 μmol/L Ang Ⅱ plus 800 μg/mL TMP	6	0.095±0.018^△
0.1 μmol/L Ang Ⅱ plus 600 μg/mL TMP	6	0.108±0.015

Group	n	Content of type Ⅰ collagen
Blank control	6	170.95±13.59
0.1 μmol/L AngⅡ	6	215.61±14.95**
0.1 μmol/L Ang Ⅱ plus 800 μg/mL TMP	6	183.09±15.13^△

Group	n	Expression level of type Ⅰ collagen mRNA
Blank control	5	0.975±0.049
0.1 μmol/L Ang Ⅱ	5	1.216±0.096*
0.1 μmol/L Ang Ⅱ plus 800 μg/mL TMP	5	0.966±0.104^△

Effects of tetramethylpyrazine on angiotensin Ⅱ-induced proliferation and type Ⅰ collagen synthesis of rat cardiac fibroblasts

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