Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (6): 548-553.doi: 10.3736/jcim20100607

• Original Experimental Research • Previous Articles     Next Articles

Effects of Radix Ginseng and Radix Notoginseng formula on expressions of vascular endothelial growth factor receptor-2 and hypoxia-inducible factor-1α in ischemic myocardium of rats with acute myocardial infarction

Xue-jun Du,Yan Lei(),Jing Yang   

  1. Department of Cardiovascular Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China
  • Received:2010-02-23 Accepted:2010-03-24 Online:2010-06-20 Published:2010-06-15
  • Contact: Yan Lei E-mail:leiy999@163.com

Objective

To investigate the effects of Radix Ginseng and Radix Notoginseng formula on expressions of vascular endothelial growth factor receptor-2 (VEGFR-2) and hypoxia-inducible factor-1α (HIF-1α) in ischemic myocardium of rats with acute myocardial infarction.
Methods

A total of 100 Wistar rats were randomly divided into normal control group, sham-operated group, untreated group, metoprolol (Betaloc) group, and high- and low-dose Radix Ginseng and Radix Notoginseng formula groups. Acute myocardial infarction was induced in the untreated group, Betaloc group, and high- and low-dose Radix Ginseng and Radix Notoginseng formula groups by left anterior descending coronary artery ligation. After 12-day treatment, microvessel density (MVD) in ischemic myocardium was detected by immunohistochemical method, while expressions of VEGFR-2 and HIF-1α proteins were detected by Western blotting, and expressions of VEGFR-2 and HIF-1α mRNAs were detected by real-time fluorescent quantitative polymerase chain reaction.
Results

MVD in the untreated group was increased significantly, higher than those in the normal control group and the sham-operated group (P<0.05) and lower than those in the high- and low-dose Radix Ginseng and Radix Notoginseng formula groups and Betaloc group (P<0.01). VEGFR-2 and HIF-1α protein and mRNA expressions in the untreated group were higher than those in the normal control group and the sham-operated group (P<0.05). VEGFR-2 and HIF-1α protein and mRNA expressions in the high- and low-dose Radix Ginseng and Radix Notoginseng formula groups and Betaloc group were higher than those in the untreated group (P<0.05). There was a significant difference between the high- and low-dose Radix Ginseng and Radix Notoginseng formula groups (P<0.05).
Conclusion

Radix Ginseng and Radix Notoginseng extract can up-regulate the protein and mRNA expressions of VEGFR-2 and HIF-1α and increase MVD in ischemic myocardium to improve myocardial ischemia so as to promote the development of collateral circulation.

Key words: Radix Ginseng, Radix Notoginseng , Vascular endothelial growth factor receptor-2, Hypoxia- inducible factor-la, Myocardial ischemia, Rats

"

Figure 1

Expressions of VEGFR-2 and HIF-1α proteins in myocardium observed by Western blotting1: Normal control group; 2: Sham-operated group; 3: Untreated group; 4: Bataloc group; 5: High-dose formula group; 6: Low-dose formula group."

"

"

[1] Wang PL, Lei Y, Lin YL, Chen KJ . Effect of serum containing Dangguibuxue decoction on expression on VEGF receptor in cultured endothelial cell[J]. Zhong Xi Yi Jie He Xin Nao Xue Guan Bing Za Zhi, 2006,4(3):209-212
王培利, 雷燕, 林燕林, 陈可冀 . 当归补血汤含药血清对培养内皮细胞生长因子受体表达的影响[J]. 中西医结合心脑血管病杂志, 2006,4(3):209-212
[2] Tian W, Lei Y, Wang SR . Effects of ginseng and Sanqi recipe on Ras-related signal protein of angiogenesis[J]. Zhongguo Zhong Xi Yi Jie He Za Zhi, 2009,29(9):802-805
田伟, 雷燕, 王硕仁 . 人参、三七组方对Ras相关信号蛋白的影响[J]. 中国中西医结合杂志, 2009,29(9):802-805
[3] Gille H, Kowalski J, Li B, LeCouter J, Moffat B, Zioncheck TF, Pelletier N, Ferrara N .Analysis of biological effects and signaling properties of Flt-1(VEGFR-1) and KDR(VEGFR-2). A reassessment using novel receptor-specific vascular endothelial growth factor mutants[J]. J Biol Chem, 2001,276(5):3222-3230
doi: 10.1074/jbc.M002016200
[4] Gerber HP, McMurtrey A, Kowalski J, Yan M, Keyt BA, Dixit V, Ferrara N .Vascular endothelial growth factor regulates endothelial cell survival through the phosphatidylinositol 3′-kinase/Akt signal transduction pathway. Requirement for Flk-1/KDR activation[J]. J Biol Chem, 1998,273(46):30336-30343
doi: 10.1074/jbc.273.46.30336
[5] Zeng H, Sanyal S, Mukhopadhyay D . Tyrosine residues 951 and 1059 of vascular endothelial growth factor receptor-2(KDR) are essential for vascular permeability factor/vascular endothelial growth factor-induced endothelium migration and proliferation, respectively[J]. J Biol Chem, 2001,276(35):32714-32719
doi: 10.1074/jbc.M103130200
[6] Yuan SH, Zhang YH, Li HX, Gao DL, Zhang L, Yin YH . The expression of hypoxia-inducible factor-1α, VEGF and MVD and their relationship with the biological behavior in breast cancer[J]. Henan Zhong Liu Xue Za Zhi, 2005,18(3):158-160
袁淑慧, 张云汉, 李惠翔, 高冬玲, 张岚, 尹玉惠 . HIF-1α、VEGF的表达及微血管密度与乳腺癌生物学行为的关系[J]. 河南肿瘤学杂志, 2005,18(3):158-160
[7] Li YK, Ning KY, Liang R, Li LD . An improved myocardial ischemia model by rat coronary artery ligature[J]. Zhongguo Xin Yao Za Zhi, 2005,14(4):427-428
李贻奎, 宁可永, 梁嵘, 李连达 . 大鼠冠状动脉结扎心肌缺血模型方法的改进[J]. 中国新药杂志, 2005,14(4):427-428
[8] Xu SY, Bian RL, Chen X. Pharmacological experimental methods(3rd ed)[M]. Beijing: People's Medical Publishing House, 2005: 1052-1053
徐叔云, 卞如濂, 陈修 . 药理实验方法学(第3版)[M]. 北京: 人民卫生出版社, 2005: 1052-1053
[9] Asahara T, Murohara T, Sullivan A, Silver M, van der Zee R, Li T, Witzenbichler B, Schatteman G, Isner JM .Isolation of putative progenitor endothelial cells for angiogenesis[J]. Science, 1997,275(5302):964-967
doi: 10.1126/science.275.5302.964
[10] Schaper W, Ito WD . Molecular mechanisms of coronary collateral vessel growth[J]. Circ Res, 1996,79(5):911-919
doi: 10.1161/01.RES.79.5.911
[11] Ng YS, Krilleke D, Shima DT . VEGF function in vascular pathogenesis[J]. Exp Cell Res, 2006,312(5):527-537
doi: 10.1016/j.yexcr.2005.11.008
[12] Neufeld G, Kessler O, Herzog Y . The interaction of neuropilin-1 and neuropilin-2 with tyrosine-kinase receptors for VEGF[J]. Adv Exp Med Biol, 2002,515:81-90
doi: 10.1007/978-1-4615-0119-0
[13] Mukherjee S, Tessema M, Wandinger-Ness A . Vesicular trafficking of tyrosine kinase receptors and associated proteins in the regulation of signaling and vascular function[J]. Circ Res, 2006,98(6):743-756
doi: 10.1161/01.RES.0000214545.99387.e3
[14] Lee SH, Wolf PL, Escudero R, Deutsch R, Jamieson SW, Thistlethwaite PA . Early expression of angiogenesis factors in acute myocardial ischemia and infarction[J]. N Engl J Med, 2000,342(9):626-633
doi: 10.1056/NEJM200003023420904
[15] Ni YB, Wang B, Xiao JG . Hypoxia-inducible factor 1 and vascular endothelial growth factor on angiogenesis[J]. Guo Wai Yi Xue Sheng Li Bing Li Ke Xue Yu Lin Chuang Fen Ce, 2002,22(3):266-269
倪永兵, 王斌, 肖继皋 . 低氧诱导因子1和血管内皮生长因子与血管新生[J]. 国外医学:生理、病理科学与临床分册, 2002,22(3):266-269
[16] Liu LX, Lu H, Luo Y, Date T, Belanger AJ, Vincent KA, Akita GY, Goldberg M, Cheng SH, Gregory RJ, Jiang C . Stabilization of vascular endothelial growth factor mRNA by hypoxia-inducible factor 1[J]. Biochem Biophys Res Commun, 2002,291(4):908-914
doi: 10.1006/bbrc.2002.6551
[17] Wang ZX, Yang LH . Hypoxia-inducible factor-1, vascular endothelial growth factor and cerebral ischemia[J]. Xue Shuan Yu Zhi Xue Xue, 2007,13(4):189-192
王忠秀, 杨林花 . HIF-1、VEGF与缺血性脑血管病[J]. 血栓与止血学, 2007,13(4):189-192
[1] Bing-rong Li, Shi-yun Shao, Long Yuan, Ru Jia, Jian Sun, Qing Ji, Hua Sui, Li-hong Zhou, Yi Zhang, Hui Liu, Qi Li, Yan Wang, Bi-meng Zhang. Effects of mild moxibustion on intestinal microbiome and NLRP3 inflammasome in rats with 5-fluorouracil-induced intestinal mucositis. Journal of Integrative Medicine, 2021, 19(2): 144-157.
[2] Sitthichai Iamsaard, Supatcharee Arun, Jaturon Burawat, Supataechasit Yannasithinon, Saranya Tongpan, Sudtida Bunsueb, Natthapol Lapyuneyong, Pannawat Choowong-in, Nareelak Tangsrisakda, Chadaporn Chaimontri, Wannisa Sukhorum. Evaluation of antioxidant capacity and reproductive toxicity of aqueous extract of Thai Mucuna pruriens seeds. Journal of Integrative Medicine, 2020, 18(3): 265-273.
[3] Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[4] Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[5] Kylie Connolly, Douglas Jackson, Candice Pullen, Andrew Fenning. Alpha-adrenoceptor antagonism by Crassostrea gigas oyster extract inhibits noradrenaline-induced vascular contraction in Wistar rats. Journal of Integrative Medicine, 2015, 13(3): 194-200.
[6] Udhaya Lavinya Baskaran, Sherry Joseph Martin, Rasool Mahaboobkhan, Sabina Evan Prince. Protective role of Triphala, an Indian traditional herbal formulation, against the nephrotoxic effects of bromobenzene in Wistar albino rats. Journal of Integrative Medicine, 2015, 13(2): 115-121.
[7] Hussein O. B. Oloyede, Matthew C. Adaja, Taofeek O. Ajiboye, Musa O. Salawu. Anti-ulcerogenic activity of aqueous extract of Carica papaya seed on indomethacin-induced peptic ulcer in male albino rats. Journal of Integrative Medicine, 2015, 13(2): 105-114.
[8] Anirudha A. Lande, Shirishkumar D. Ambavade, Uma S. Swami, Prafulla P. Adkara Prashant D. Ambavade, Arun B. Waghamare. Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase. Journal of Integrative Medicine, 2015, 13(1): 25-33.
[9] Elizabeth Abidemi Balogun, Sylvia Orume Malomo, Joseph Oluwatope Adebayo, Ahmed Adebayo Ishola, Ayodele Olufemi Soladoye, Lawrence Aderemi Olatunji, Olatunji Matthew Kolawole, Stephen Olubunmi Oguntoye, Abiola Samuel Babatunde, Oluwole Busayo Akinola . In vivo antimalarial activity and toxicological effects of methanolic extract of Cocos nucifera (Dwarf red variety) husk fibre. Journal of Integrative Medicine, 2014, 12(6): 504-511.
[10] Urmila Aswar, Mayuri Gurav, Ganesh More, Khaled Rashed, Manoj Aswar. Effect of aqueous extract of Solanum xanthocarpum Schrad. & Wendl. on postmenopausal syndrome in ovariectomized rats. Journal of Integrative Medicine, 2014, 12(5): 439-446.
[11] Xin-fang Zhang, Ji Zhu, Wen-ye Geng, Shu-jun Zhao, Chuan-wei Jiang, Sheng-rong Cai, Miao Cheng, Chuan-yun Zhou, Zi-bing Liu. Electroacupuncture at Feishu (BL13) and Zusanli (ST36) down-regulates the expression of orexins and their receptors in rats with chronic obstructive pulmonary disease. Journal of Integrative Medicine, 2014, 12(5): 417-424.
[12] Benjamin Perry, Junzeng Zhang, Tarek Saleh, Yanwen Wang. Liuwei Dihuang, a traditional Chinese herbal formula, suppresses chronic inflammation and oxidative stress in obese rats. Journal of Integrative Medicine, 2014, 12(5): 447-454.
[13] Upendarrao Golla, Praveen Kumar Gajam, Solomon Sunder Bhimathati. Evaluation of diuretic and laxative activity of hydro-alcoholic extract of Desmostachya bipinnata (L.) Stapf in rats. Journal of Integrative Medicine, 2014, 12(4): 372-378.
[14] Qian Zhang, Xin-hua Xiao, Ming Li, Wen-hui Li, Miao Yu, Hua-bing Zhang, Fan Ping, Zhi-xin Wang, Jia Zheng ​. Chromium-containing traditional Chinese medicine, Tianmai Xiaoke Tablet improves blood glucose through activating insulin-signaling pathway and inhibiting PTP1B and PCK2 in diabetic rats. Journal of Integrative Medicine, 2014, 12(3): 162-170.
[15] Amir Rashidian​, Saeed Mehrzadi, Ali Reza Ghannadi, Parvin Mahzooni, Samira Sadr, Mohsen Minaiyan​. Protective effect of ginger volatile oil against acetic acid-induced colitis in rats: A light microscopic evaluation. Journal of Integrative Medicine, 2014, 12(2): 115-120.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[2] Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[3] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[4] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[5] Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[6] Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[7] Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[8] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[9] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[10] Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239