Search JIM Advanced Search

Journal of Integrative Medicine ›› 2014, Vol. 12 ›› Issue (1): 59-65.doi: 10.1016/S2095-4964(14)60006-6

• Research Article • Previous Articles     Next Articles

Anti-fatty liver effects of oils from Zingiber officinale and Curcuma longa on ethanol-induced fatty liver in rats

Sarah Onyenibe Nwozoa, Damilola Adeola Osunmadewaa, Babatunji Emmanuel Oyinloyea,b   

  1. Nutritional and Industrial Research Laboratories, Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan 200002, Nigeria
    Department of Biochemistry, College of Sciences, Afe Babalola University, Ado Ekiti 360001, Nigeria
  • Received:2013-10-31 Accepted:2013-12-20 Online:2014-01-10 Published:2014-01-15

Objective

The present study is aimed at evaluating the protective effects of oils from Zingiber officinale (ginger) and Curcuma longa (turmeric) on acute ethanol-induced fatty liver in male Wistar rats. 

Methods

Ferric reducing antioxidant power activity and oxygen radical absorbance capacity of the oils were evaluated ex vivo. Rats were pretreated for 28 d with standard drug (Livolin Forte) and oils from Z. officinale and C. longa before they were exposed to 45% ethanol (4.8 g/kg) to induce acute fatty liver. Histological changes were observed and the degree of protection was measured by using biochemical parameters such as alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase activities. Serum triglyceride (TG) level, total cholesterol (TC) level and the effects of both oils on reduced gluthatione (GSH), glutathione-S-transferase (GST), superoxide dismutase (SOD) and hepatic malondialdehyde (MDA) levels were estimated. 

Results

Oils from Z. officinale and C. longa at a dose of 200 mg/kg showed hepatoprotection by decreasing the activities of serum enzymes, serum TG, serum TC and hepatic MDA, while they significantly restored the level of GSH as well as GST and SOD activities. Histological examination of rats tissues was related to the obtained results. 

Conclusion

From the results it may be concluded that oils from Z. officinale and C. longa (200 mg/kg) exhibited hepatoprotective activity in acute ethanol-induced fatty liver and Z. officinale oil was identified to have better effects than C. longa oil.

Key words: Curcuma longa, Zingiber officinale, Plant extracts, Fatty liver, In vivo

"

"

"

Figure 1

Representative histological section of liver in acute toxicity (Light microscopy, ×400; haematoxylin and eosin staining) A: Group I, no visible lesions seen. B: Group II, there is marked portal congestion and cellular infiltration by mononuclear cells. C: Group III, there are periportal hydropic degeneration of hepatocytes with cellular infiltration by mononuclear cells. D: Group IV, there are mild portal congestion and mononuclear cellular infiltration, and mild hydropic degeneration. E: Group V, there are mild portal congestion, mild hydropic degeneration and few foci of cellular infiltration by mononuclear cells."

[1] Rukkumani R, Aruna K, Varma PS, Menon VP . Influence of ferulic acid on circulatory prooxidant-antioxidant status during alcohol and PUFA induced toxicity[J]. J Physiol Pharmacol, 2004,55(3):551-561
[2] Reddy JK, Rao MS . Lipid metabolism and liver inflammation. II. Fatty liver disease and fatty acid oxidation[J]. Am J Physiol Gastrointest Liver Physiol, 2006,290(5):G852-G858
doi: 10.1152/ajpgi.00521.2005
[3] Rao MS, Reddy JK . PPARalpha in the pathogenesis of fatty liver disease[J]. Hepatology, 2004,40(4):783-786
doi: 10.1002/hep.1840400405 pmid: 15382158
[4] Albano E, Vidali M . Immune mechanisms in alcoholic liver disease[J]. Genes Nutr, 2010,5(2):141-147
doi: 10.1007/s12263-009-0151-4 pmid: 19809845
[5] Lv X, Chen Z, Li J, Zhang L, Liu H, Huang C, Zhu P . Caffeine protects against alcoholic liver injury by attenuating inflammatory response and oxidative stress[J]. Inflamm Res, 2010,59(8):635-645
doi: 10.1007/s00011-010-0176-6
[6] Nakbi A, Tayeb W, Grissa A, Issaoui M, Dabbou S, Chargui I, Ellouz M, Miled A, Hammami M . Effects of olive oil and its fractions on oxidative stress and the liver’s fatty acid composition in 2,4-dichlorophenoxyacetic acid-treated rats[J]. Nutr Metab (Lond), 2010,7:80
doi: 10.1186/1743-7075-7-80
[7] Al-Said MS, Mothana RA, Al-Yahya MA, Al-Blowi AS, Al-Sohaibani M, Ahmed AF, Rafatullah S . Edible oils for liver protection: hepatoprotective potentiality of Moringa oleifera seed oil against chemical-induced hepatitis in rats[J]. J Food Sci, 2012,77(7):T124-T130
doi: 10.1111/jfds.2012.77.issue-7
[8] Hamed MA, El-Rigal NS, Ali SA . Effects of black seed oil on resolution of hepato-renal toxicity induced by bromobenzene in rats[J]. Eur Rev Med Pharmacol Sci, 2013,17(5):569-581
[9] Salama SM, Abdulla MA , AlRashdi AS, Ismail S, Alkiyumi SS, Golbabapour S.Hepatoprotective effect of ethanolic extract of Curcuma longa on thioacetamide induced liver cirrhosis in rats[J]. BMC Complement Altern Med, 2013,13:56
doi: 10.1186/1472-6882-13-56
[10] Maizura M, Aminah A, Wan Aida WM . Total phenolic content and antioxidant activity of kesum(Polygonum minus), ginger(Zingiber officinale) and turmeric(Curcuma longa) extract[J]. Int Food Res J, 2011,18:526-531
[11] Miquel J, Bernd A, Sempere JM, Díaz-Alperi J, Ramírez A . The curcuma antioxidants: pharmacological effects and prospects for future clinical use. A review[J]. Arch Gerontol Geriatr, 2002,34(1):37-46
doi: 10.1016/S0167-4943(01)00194-7
[12] Joe B, Vijaykumar M, Lokesh BR . Biological properties of curcumin — cellular and molecular mechanisms of action[J]. Crit Rev Food Sci Nutr, 2004,44(2):97-111
doi: 10.1080/10408690490424702
[13] Ajith TA, Aswathy MS, Hema U . Protective effect of Zingiber officinale roscoe against anticancer drug doxorubicin-induced acute nephrotoxicity[J]. Food Chem Toxicol, 2008,46(9):3178-3181
doi: 10.1016/j.fct.2008.07.004
[14] Ahmad N, Sulaiman S, Mukti NA, Murad NA, Hamid NAA, Yusof YAM . Effects of ginger extract(Zingiber offincinale roscoe) on antioxidant status of hepatocarcinoma Indu[J]. Malaysian J Biochem Mol Biol, 2006,14:7-12
[15] Akhani SP, Vishwakarma SL, Goyal RK . Anti-diabetic activity of Zingiber officinale in streptozotocin-induced type I diabetic rats[J]. J Pharm Pharmacol, 2004,56(1):101-105
doi: 10.1211/0022357022403 pmid: 14980006
[16] Yemitan OK, Izegbu MC . Protective effects of Zingiber officinale(Zingiberaceae) against carbon tetrachloride and acetaminophen-induced hepatotoxicity in rats[J]. Phytother Res, 2006,20(11):997-1002
doi: 10.1002/(ISSN)1099-1573
[17] Young HY, Luo YL, Cheng HY, Hsieh WC, Liao JC, Peng WH . Analgesic and anti-inflammatory activities of [6]-gingerol[J]. J Ethnopharmacol, 2005,96(1-2):207-210
doi: 10.1016/j.jep.2004.09.009 pmid: 15588672
[18] Benzie IF, Strain JJ . The ferric reducing ability of plasma(FRAP) as a measure of “antioxidant power”: the FRAP assay[J]. Anal Biochem, 1996,239(1):70-76
doi: 10.1006/abio.1996.0292
[19] Ou B, Hampsch-Woodill M, Prior RL . Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe[J]. J Agric Food Chem, 2001,49(10):4619-4626
doi: 10.1021/jf010586o
[20] Varshney R, Kale RK . Effects of calmodulin antagonists on radiation-induced lipid peroxidation in microsomes[J]. Int J Radiat Biol, 1990,58(5):733-743
doi: 10.1080/09553009014552121 pmid: 1977818
[21] Misra HP, Fridovich I . The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase[J]. J Biol Chem, 1972,247(10):3170-3175
[22] Beutler E, Duron O, Kelly BM . Improved method of determination of blood glutathione[J]. J Lab Clin Med, 1963,61:882-890
[23] Habig WH, Pabst MJ, Jakoby WB.Glutathione S-transferases . The first enzymatic step in mercapturic acid formation[J]. J Biol Chem, 1974,249(22):7130-7139
[24] Brenner DA, Sigmund S . Pathogenesis of alcoholic hepatitis[J]. J Gastroentol Hepatol, 2004,19(S7):S229-S235
doi: 10.1111/jgh.2004.19.issue-s7
[25] Rouach H, Fataccioli V, Gentil M, French SW, Morimoto M, Nordmann R . Effect of chronic ethanol feeding on lipid peroxidation and protein oxidation in relation to liver pathology[J]. Hepatology, 1997,25(2):351-355
doi: 10.1002/(ISSN)1527-3350
[26] Fernández-Checa JC, Kaplowitz N, Colell A, García-Ruiz C . Oxidative stress and alcoholic liver disease[J]. Alcohol Health Res World, 1997,21(4):321-324
doi: 10.1055/s-0029-1214370 pmid: 19387914
[27] Dey A, Cederbaum AI . Alcohol and oxidative liver injury[J]. Hepatology, 2006,43(2 Suppl 1):S63-S74
doi: 10.1002/(ISSN)1527-3350
[28] Lee KJ, Choi CY, Chung YC, Kim YS, Ryu SY, Roh SH, Jeong HG . Protective effect of saponins derived from roots of Platycodon grandiflorum on tert-butyl hydroperoxide-induced oxidative hepatotoxicity[J]. Toxicol Lett, 2004,147(3):271-282
doi: 10.1016/j.toxlet.2003.12.002
[29] Goldberg DM, Watts C . Serum enzyme changes as evidence of liver reaction to oral alcohol[J]. Gastroenterology, 1965,49(3):256-261
[30] Yanardag R, Ozsoy-Sacan O, Bolkent S, Orak H, Karabulut-Bulan O . Protective effects of metformin treatment on the liver injury of streptozotocin-diabetic rats[J]. Hum Exp Toxicol, 2005,24(3):129-135
doi: 10.1191/0960327104ht507oa
[31] Nwozo SO, Oyinloye BE . Hepatoprotective effect of aqueous extract of Aframomum melegueta on ethanol-induced toxicity in rats[J]. Acta Biochim Pol, 2011,58(3):355-358
[32] Raghavendran HRB, Sathivel A, Devaki T . Hepatoprotective nature of seaweed alcoholic extract on acetaminophen induced hepatic oxidative stress[J]. J Health Sci, 2004,50(1):42-46
doi: 10.1248/jhs.50.42
[33] Schlorff EC, Husain K, Somani SM . Dose- and time-dependent effects of ethanol on plasma antioxidant system in rat[J]. Alcohol, 1999,17(2):97-105
doi: 10.1016/S0741-8329(99)00004-X pmid: 10456572
[34] Rukkumani R, Aruna K, Varma PS, Rajasekaran KN, Menon VP . Comparative effects of curcumin and an analog of curcumin on alcohol and PUFA induced oxidative stress[J]. J Pharm Pharm Sci, 2004,7(2):274-283
[35] Antonenko VD, Popova SV, Panchenko LF . Effect of ethanol and clofibrate on the activity of lipid catabolism enzyme systems in rat liver[J]. Farmakol Toksikol, 1983,46(5):86-90
[36] Fielding BA, Reid G, Grady M, Humphreys SM, Evans K, Frayn KN . Ethanol with a mixed meal increases postprandial triacylglycerol but decreases postprandial non-esterified fatty acid concentrations[J]. Br J Nutr, 2000,83(6):597-604
doi: 10.1017/S0007114500000763
[37] Weber LW, Boll M, Stampfl A . Hepatotoxicity and mechanism of action of haloalkanes: carbon tetrachloride as a toxicological model[J]. Crit Rev Toxicol, 2003,33(2):105-136
doi: 10.1080/713611034
[38] El-Sayed IH, Lotfy M, El-Khawaga OA, Nasif WA, El-Shahat M . Prominent free radicals scavenging activity of tannic acid in lead-induced oxidative stress in experimental mice[J]. Toxicol Ind Health, 2006,22(4):157-163
doi: 10.1191/0748233706th256oa
[39] Fridovich I . Superoxide anion radical(O2-.), superoxide dismutases, and related matters[J]. J Biol Chem, 1997,272(30):18515-18517
doi: 10.1074/jbc.272.30.18515
[40] Jurczuk M, Moniuszko-Jakoniuk J, Brzóska MM . Involvement of some low-molecular thiols in the peroxidative mechanisms of lead and ethanol action on rat liver and kidney[J]. Toxicology, 2006,219(1-3):11-21
doi: 10.1016/j.tox.2005.10.022
[1] Nadiah Razali, Aidiahmad Dewa, Mohd Zaini Asmawi, Nornisah Mohamed, Nurul Maizan Manshor. Mechanisms underlying the vascular relaxation activities of Zingiber officinale var. rubrum in thoracic aorta of spontaneously hypertensive rats. Journal of Integrative Medicine, 2020, 18(1): 46-58.
[2] Stefania Lamponi, Anna Maria Aloisi, Claudia Bonechi, Marco Consumi, Alessandro Donati, Gemma Leone, Claudio Rossi, Gabriella Tamasi, Luana Ghiandai, Ersilia Ferrini, Paolo Fiorenzani, Ilaria Ceccarelli, Agnese Magnani. Evaluation of in vitro cell and blood compatibility and in vivo analgesic activity of plant-derived dietary supplements. Journal of Integrative Medicine, 2019, 17(3): 213-220.
[3] Mary Abiola Okesola, Basiru Olaitan Ajiboye, Babatunji Emmanuel Oyinloye, Oluwafemi Adeleke Ojo. Neuromodulatory effects of ethyl acetate fraction of Zingiber officinale Roscoe extract in rats with lead-induced oxidative stress. Journal of Integrative Medicine, 2019, 17(2): 125-131.
[4] Alexandra R. Vaughn, Aunna Pourang, Ashley K. Clark, Waqas Burney, Raja K. Sivamani . Dietary supplementation with turmeric polyherbal formulation decreases facial redness: a randomized double-blind controlled pilot study. Journal of Integrative Medicine, 2019, 17(1): 20-23.
[5] Rohitash Jamwal. Bioavailable curcumin formulations: A review of pharmacokinetic studies in healthy volunteers. Journal of Integrative Medicine, 2018, 16(6): 367-374.
[6] Supakanya Kumkarnjana, Rutt Suttisri, Ubonthip Nimmannit, Thongchai Koobkokkruad, Chutichot Pattamadilok, Nontima Vardhanabhuti . Anti-adipogenic effect of flavonoids from Chromolaena odorata leaves in 3T3-L1 adipocytes. Journal of Integrative Medicine, 2018, 16(6): 427-434.
[7] Shohini Chakraborty, Nashra Afaq, Neelam Singh, Sukanta Majumdar. Antimicrobial activity of Cannabis sativaThuja orientalis and Psidium guajava leaf extracts against methicillin-resistant Staphylococcus aureus. Journal of Integrative Medicine, 2018, 16(5): 350-357.
[8] Bruno José Martins Da Silva, Amanda Anastácia Pinto Hage, Edilene Oliveira Silva, Ana Paula Drummond Rodrigues. Medicinal plants from the Brazilian Amazonian region and their antileishmanial activity: A review. Journal of Integrative Medicine, 2018, 16(4): 211-222.
[9] Sahar Dehdari, Homa Hajimehdipoor, Somayeh Esmaeili, Rasool Choopani, Seyed Alireza Mortazavi. Traditional and modern aspects of hemorrhoid treatment in Iran: A review. Journal of Integrative Medicine, 2018, 16(2): 90-98.
[10] Javed Yakoob, Zaigham Abbas, Malik Hassan Mehmood, Kanwal Tariq, Saima Azhar Saleem, Safia Awan, Abdul Malik, Saeed Hamid, Rustam Khan, Wasim Jafri. Helicobacter pylori outer membrane protein Q genotypes and their susceptibility to anti-adhesive phytotherapeutic agents. Journal of Integrative Medicine, 2017, 15(5): 398-406.
[11] Nisha Panth, Keshav Raj Paudel, Rajendra Karki. Phytochemical profile and biological activity of Juglans regia. Journal of Integrative Medicine, 2016, 14(5): 359-373.
[12] Musa Toyin Yakubu, Abdulquadri Funmbi Olutoye. Aphrodisiac activity of aqueous extract of Anthonotha macrophylla P. Beauv. leaves in female Wistar rats. Journal of Integrative Medicine, 2016, 14(5): 400-408.
[13] JiaXia, Li-qunHe, XiaoSu. Interventional mechanisms of herbs or herbal extracts on renal interstitial fibrosis. Journal of Integrative Medicine, 2016, 14(3): 165-173.
[14] MesfinYimam, Young-Chul Lee, Breanna Moore, Ping Jiao, MeiHong, Jeong-Bum Nam, Mi-Ran Kim, Eu-Jin Hyun, Min Chu, Lidia Brownell, Qi Jia. Analgesic and anti-inflammatory effects of UP1304, a botanical composite containing standardized extracts of Curcuma longa and Morus alba. Journal of Integrative Medicine, 2016, 14(1): 60-68.
[15] Wei Chiang Chan Eric, Kuin Wong Siu. Phytochemistry and pharmacology of ornamental gingers, Hedychium coronarium and Alpinia purpurata: A review. Journal of Integrative Medicine, 2015, 13(6): 368-379.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[2] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[3] Hai-feng Wei, Bai-liu Ya, Ling Zhao, Cui-fei Ye, Li Zhang, Lin Li. Evaluation of tongue manifestation of blood stasis syndrome and its relationship with blood rheological disorder in a rat model of transient brain ischemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 73-76
[4] Xi Lin, Jian-ping Liu. Herbal medicines for viral myocarditis. Journal of Chinese Integrative Medicine, 2008, 6(1): 76
[5] A-gao Zhou, Yong Zhang, Gang Kui, De-Yun Kong, Hai-liang Ge, Qiu-hua Ren, Jia-rong Dong, Sheng Hong, Xu-ming Mao, Yin Wang, Hui-zheng Zhang, Shu-jun Wang. Influence of traditional Chinese compound recipes with different efficacy on body weight, tumor weight and immune function in H22 cancer-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 77-82
[6] Xi Lin, Jian-ping Liu. Tai chi for treating rheumatoid arthritis. Journal of Chinese Integrative Medicine, 2008, 6(1): 82
[7] Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[8] Li Zhou, Hong-xing Zhang, Ling-guang Liu, Wen-jun Wan. Effect of electro-acupuncture at Fenglong (GV 16) on nitric oxide and endothelin in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 89-92
[9] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. Guiding principles of clinical research on mild cognitive impairment (protocol). Journal of Chinese Integrative Medicine, 2008, 6(1): 9-14
[10] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013