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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (4): 278-281.doi: 10.3736/jcim20050409

• Original Experimental Research • Previous Articles     Next Articles

Effects of Fuzheng Yiliu Granule-medicated serum on apoptosis of liver cancer cells from mice and its mechanism

Jian-xiong Zhao,Wei-dong Cheng,Rui-feng Xu,Yuan-qing Li   

  1. Institute of Integrated Traditional Chinese and Western Medicine, Lanzhou Medical College, Lanzhou, Gansu Province 730000, China
  • Received:2004-12-18 Online:2005-07-20 Published:2005-07-20
  • Contact: Jian-xiong Zhao E-mail:ZhaoJX-01@126.com

Objective

To study the effects of Fuzheng Yiliu Granule (FZYLG)-medicated serum on apoptosis of liver cancer cells H22 from mice and its mechanism.

Methods

Liver cancer cells H22 from mice were incubated in culture media containing sera from rabbits medicated with different doses of FZYLG. Flow cytometry was used to examine the cell cycle and analyze the apoptotic rate of the H22 cells. The morphological changes of the H22 cells were observed by transmission electron microscope and the apoptosis related proteins Bcl-2 and Bax were examined by streptavidin-biotin peroxidase complex (SABC) method.

Results

FZYLG-medicated serum could influence the cell cycle and stop the proliferation of H22 cells at the G1/G0 phase with apoptotic peak being detected. In culture media with FZYLG-medicated sera, the expression of Bcl-2 decreased while that of Bax increased as compared with that in culture medium with non-medicated serum (P<0.05).

Conclusion

FZYLG-medicated serum can induce apoptosis of the liver cancer cells H22 by influencing the cell cycle, down-regulating the expression of Bcl-2 and up-regulating the expression of Bax.

Key words: Fuzheng Yiliu Granules, Apoptosis, cell cycle, Proto-oncogene proteins c-bcl-2, Bax

CLC Number: 

  • R735.7

Tab 1

Effects of different culture media on cell cycle and apoptosis of H22 cells ($\bar{x}$±s, %)"

Culture medium n Apoptotic rate G0/G1 phase S phase G2/M phase
With non-medicated serum 8 5.5±1.3 66.5±5.7 23.0±2.8 11.2±1.2
With low-dose FZYLG-medicated serum 8 25.5±4.7△△ 67.2±6.4 23.2±2.4** 8.8±3.7**
With medium-dose FZYLG-medicated serum 8 30.9±4.1△△ 72.8±4.3△△ 19.3±3.9** 7.6±3.1**
With high-dose FZYLG-medicated serum 8 39.6±5.9*△△ 78.6±6.1△△ 16.8±2.1** 3.7±1.3**
With Tianxian Capsule-medicated serum 8 31.1±4.2△△ 70.9±4.1 3.1±1.8 25.9±5.5

Tab 2

Effects of different culture media on expressions of Bcl-2 and Bax in H22 cells"

Culture medium Bcl-2 Bax
+ ++ u P + ++ u P
With non-medicated serum 3 3 9 11 1 3
With low-dose FZYLG-medicated serum 4 3 8 103.5 >0.05 10 4 1 110.5 >0.05
With medium-dose FZYLG-medicated serum 5 9 1 58.5 <0.05 5 4 6 70.5 >0.05
With high-dose FZYLG-medicated serum 7 7 1 52.5 <0.05 2 3 10 44.5 <0.05
With Tianxian Capsule-medicated serum 5 3 7 94.5 >0.05 5 5 5 71.0 >0.05

Fig 1

Ultrastructure of cells cultured in medium with non-medicated serum examined by an electron microscope (×4000)"

Fig 2

Ultrastructure of cells cultured in medium with medium-dose FZYLG-medicated serum examined by an electron microscope (×4000)"

[1] 戴恩来, 赵健雄, 朱玉真 , 等. 扶正抑瘤汤对肿瘤细胞周期及端粒酶影响的实验研究[J]. 中国中西医结合杂志, 2001,21(10):760-762
doi: 10.7661/CJIM.2001.10.760
[2] 赵健雄, 曲勇, 陈学忠 , 等. 扶正抑瘤颗粒对食管癌、胃癌组织细胞周期及核转录因子的影响[J]. 中国中西医结合杂志, 2003,23(12):908-910
[3] 生秀杰, 宋和存, 何秀琴 . 抗凋亡基因Bcl-2在子宫内膜癌的表达及意义[J]. 中华肿瘤杂志, 1998,20(5):365-366
doi: 10.3760/j.issn:0253-3766.1998.05.014
[4] Alison MR, Sarraf CE . Apoptosis: regulation and relevance to toxicology[J]. Hum Exp Toxicol, 1995,14(3):234-247
doi: 10.1177/096032719501400302 pmid: 7779453
[5] 周俊 . 中药复方——天然组合化学库与多靶作用机理[J]. 中国中西医结合杂志, 1998,18(2):67
[6] Krajewski S, Krajewska M, Shabaik A , et al. Immunohistochemical determination of in vivo distribution of Bax, a dominant inhibitor of Bcl-2[J]. Am J Pathol, 1994,145(6):1323-1336
doi: 10.1097/00000433-199412000-00015 pmid: 7992838
[7] 杨雁, 陈敏珠 . 黄芪总苷对肝癌细胞凋亡及wtp53基因表达的影响[J]. 中国药理学通报, 2001,17(4):447-451
[8] 杨铁虹, 卢保华, 贾敏 , 等. 当归多糖对小鼠免疫功能的影响[J]. 中国药理学通报, 2003,19(4):448-451
[9] 袁静, 顾振伦, 周文轩 , 等. 榄香烯诱导人白血病K562细胞凋亡及调控Bcl-2蛋白的表达[J]. 中国药理学报, 1999,20(2):103-106
[10] 唐清秀 . 墓头回对小鼠免疫功能的影响[J]. 中国中医基础医学杂志, 1998,4(4):27-28
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