Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (4): 290-293.doi: 10.3736/jcim20050413

• Original Experimental Research • Previous Articles     Next Articles

Effects of Huatan Xiezhuo Recipe on insulin resistance and leptin in rats with fatty liver

Fang Ye1,Wen-xia Zhao2(),Ming-san Miao2,Jian-guo Li2,Yong-yan Zhang2   

  1. 1. First Clinical College, Nanjing University of Traditional Chinese Medicine, Nanjing, Jiangsu Province 210029, China
    2. Institute of Liver Diseases, Henan College of Traditional Chinese Medicine, Zhengzhou, Henan Province 450008, China
  • Received:2004-09-20 Online:2005-07-20 Published:2005-07-20
  • Contact: Wen-xia Zhao


To observe the effects of Huatan Xiezhuo Recipe (HTXZR) on fatty liver of rats.


Forty-eight SD rats were randomly divided into five groups: normal control group, untreated group, Dongbao Gantai Tablet (DBGTT)-treated group, low-dose HTXZR-treated group and high-dose HTXZR-treated group. Fatty liver was induced in the rats by hyperlipid diet and intraperitoneal injection of tetracycline. The pathological changes of liver tissues in rats were observed, and the liver function, serum leptin, insulin resistance index, triglyceride (TG) and free fatty acid (FFA) in the liver were detected.


The levels of serum leptin, insulin resistance index, FFA and TG in rats of the three treated groups were lower than those of the untreated group (P<0.01), and such effects in the HTXZR-treated groups were more significant than those in the DBGTT-treated group (P<0.05).


The main action mechanisms of HTXZR in treating fatty liver are probably to promote the lipid metabolism, decrease the leptin and insulin resistance.

Key words: Huatan Xiezhuo Recipe, Fatty liver, Free fatty acid, Insulin resistance, Leptin resistance

CLC Number: 

  • R589.2

Tab 1

Changes of liver weight and liver index of the rats ($\bar{x}$±s)"

Group n Body weight (g) Liver index (%)
Normal control group 8 275.8±7.9 3.02±0.17
Untreated group 9 333.6±15.2▲▲ 4.10±0.24▲▲
DBGTT-treated group 10 311.0±10.7*▲▲ 3.80±0.20*▲▲
Low-dose HTXZR-treated group 10 300.6±12.8*▲▲ 3.55±0.17*△▲▲
High-dose HTXZR-treated group 10 283.5±11.1*▲▲ 3.68±0.16*▲▲

Tab 2

Changes of serum ALT, AST, leptin and insulin resistance index of the rats ($\bar{x}$±s)"

Group n ALT (U/L) AST (U/L) Leptin (μg/L) Insulin resistance index
Normal control group 8 48.88±4.76 149.75±5.37 0.29±0.06 9.94±0.81
Untreated group 9 425.87±72.93 292.12±30.99 1.08±0.37 14.69±1.93
DBGTT-treated group 10 212.13±19.17* 249.63±27.60 0.86±0.20 12.44±1.62*
Low-dose HTXZR-treated group 10 167.11±24.44*△△ 188.44±25.52*△△ 0.69±0.19* 14.10±2.63
High-dose HTXZR-treated group 10 150.88±19.31**△△ 204.50±15.90**△△ 0.62±0.16* 12.84±1.79*

Tab 3

Changes of liver tissue FFA, TG and serum TG of the rats ($\bar{x}$±s)"

Group n Liver homogenate FFA
Liver omogenate TG
Serum TG
Normal control group 8 123.71±16.37 0.56±0.05 0.85±0.19
Untreated group 9 560.50±81.67 7.38±0.55 1.49±0.45
DBGTT-treated group 10 382.73±36.71** 5.19±0.52** 1.47±0.44
Low-dose HTXZR-treated group 10 324.46±34.51**△△ 4.68±0.43** 1.26±0.34
High-dose HTXZR-treated group 10 280.51±14.78**△△ 3.77±0.31**△△ 1.43±0.31
[1] 杨正武, 魏良州 . 瘦素抵抗、胰岛素抵抗与非酒精性脂肪肝病[J]. 医学综述, 2004,10(6):335-338
doi: 10.3969/j.issn.1006-2084.2004.06.008
[2] 吴木潮 . 瘦素与代谢综合征[J]. 国外医学·内科学分册, 2000,27(12):519-521
[3] 钟岚, 范建高, 王国良 , 等. 肥胖、高脂血症性脂肪肝肝炎模型的建立[J]. 实验动物科学与管理, 2000,17(2):16-20
[4] 李光伟 . 当前胰岛素敏感性评估及胰岛素抵抗研究的某些误区[J]. 中华内科杂志, 1998,37(2):81-83
[5] 孙丽伟, 陈芝芸, 严茂祥 , 等. 高脂饮食脂肪肝大鼠血清TNF-α、IL-6、Leptin的变化[J]. 浙江中医学院学报, 2004,28(3):41-42
[6] 梁坚, 王婉梅, 揭育丽 , 等. 脂肪肝患者血清瘦素与胰岛素抵抗的关系[J]. 中国临床康复, 2004,8(27):5847-5849
[7] 黄伟, 季峰, 姜玲玲 , 等. 脂肪肝患者血清瘦素水平检测及其与发病的关系[J]. 中华检验医学杂志, 2004,27(2):106
[1] Meng-ya Shan, Ying Dai, Xiao-dan Ren, Jing Zheng, Ke-bin Zhang, Bin Chen, Jun Yan, Zi-hui Xu. Berberine mitigates nonalcoholic hepatic steatosis by downregulating SIRT1-FoxO1-SREBP2 pathway for cholesterol synthesis. Journal of Integrative Medicine, 2021, 19(6): 545-554.
[2] Mao-xing Pan, Chui-yang Zheng, Yuan-jun Deng, Kai-rui Tang, Huan Nie, Ji-qian Xie, Dong-dong Liu, Gui-fang Tu, Qin-he Yang, Yu-pei Zhang. Hepatic protective effects of Shenling Baizhu powder, a herbal compound, against inflammatory damage via TLR4/NLRP3 signalling pathway in rats with nonalcoholic fatty liver disease . Journal of Integrative Medicine, 2021, 19(5): 428-438.
[3] Jafar Abolghasemi, Mohammad Ali Farboodniay Jahromi, Mohammad Hossein Sharifi, Zohreh Mazloom, Leila Hosseini, Nasrindokht Zamani, Majid Nimrouzi. Effects of Zataria oxymel on obesity, insulin resistance and lipid profile: A randomized, controlled, triple-blind trial. Journal of Integrative Medicine, 2020, 18(5): 401-408.
[4] Esmaeil Yousefi Rad, Behzad Nazarian, Somayeh Saboori, Ebrahim Falahi, Azita Hekmatdoost. Effects of L-arginine supplementation on glycemic profile: Evidence from a systematic review and meta-analysis of clinical trials. Journal of Integrative Medicine, 2020, 18(4): 284-291.
[5] Sonia Chew Wen Phang, Uma Devi Palanisamy, Khalid Abdul Kadir. Effects of geraniin (rambutan rind extract) on blood pressure and metabolic parameters in rats fed high-fat diet. Journal of Integrative Medicine, 2019, 17(2): 100-106.
[6] Mehranghiz Ebrahimi-Mameghani, Mohammad Asghari-Jafarabadi, Khatereh Rezazadeh. TCF7L2-rs7903146 polymorphism modulates the effect of artichoke leaf extract supplementation on insulin resistance in metabolic syndrome: A randomized, double-blind, placebo-controlled trial. Journal of Integrative Medicine, 2018, 16(5): 329-334.
[7] Nazli Samadi, Hassan Mozaffari-Khosravi, Masoud Rahmanian, Mohsen Askarishahi. Effects of bee propolis supplementation on glycemic control, lipid profile and insulin resistance indices in patients with type 2 diabetes: A randomized, double-blind clinical trial. Journal of Integrative Medicine, 2017, 15(2): 124-134.
[8] Sarah Onyenibe Nwozo, Damilola Adeola Osunmadewa, Babatunji Emmanuel Oyinloye. Anti-fatty liver effects of oils from Zingiber officinale and Curcuma longa on ethanol-induced fatty liver in rats. Journal of Integrative Medicine, 2014, 12(1): 59-65.
[9] Tian-zhan Wang, Yu Chen, Yan-ming He, Xiao-dong Fu, Wang Yi, Yan-qiu Xu, Hong-jie Yang, Hong-li Xue, Yi Liu, Xiao-tao Feng, Teng Zhang, Wen-jian Wang. Effects of Chinese herbal medicine Yiqi Huaju Qingli Formula in metabolic syndrome patients with microalbuminuria: a randomized placebo-controlled trial. Journal of Chinese Integrative Medicine, 2013, 11(3): 175-183.
[10] Wen-jian Wang. Enhancing the treatment of metabolic syndrome with integrative medicine. Journal of Chinese Integrative Medicine, 2013, 11(3): 153-156.
[11] Di Li, Guan-liang Wang, Meng-ya Shan, Jia-han Liu, Lin Wang, De-zeng Zhu. Effects of ursolic acid on c-Cbl-associated protein expression in 3T3-L1 adipocytes with insulin resistance. Journal of Chinese Integrative Medicine, 2012, 10(8): 886-893.
[12] Dong-mei Zhang, Li-xia Lou, Ai-ming Wu, Xi-ying Lü, Zhao-juan Hu, Yan-hong Zhang, Hong-fang Liu. Effects of Astragalus membranaceus and Potentilla discolor mixture on insulin resistance and its related mRNA expressions in KKAy mice with type 2 diabetes. Journal of Chinese Integrative Medicine, 2012, 10(7): 821-826.
[13] Lin Wang, Guan-liang Wang, Jia-han Liu, Di Li, De-zeng Zhu, Liang-neng Wu . Effects of ursolic acid in ameliorating insulin resistance in liver of KKAy mice via peroxisome proliferator-activated receptors α and γ. Journal of Chinese Integrative Medicine, 2012, 10(7): 793-799.
[14] Tao Liu , Li-li Yang , Li Zhang, Hai-yan Song , Dong-fei Li , Guang li. Comparative study on the effects of different therapeutic methods in preventing and treating nonalcoholic fatty liver in rats. Journal of Chinese Integrative Medicine, 2012, 10(10): 1120-1126.
[15] Li-li Yang, Miao Wang, Tao Liu, Hai-yan Song, Dong-fei Li, Pei-yong Zheng, Ping Liu, Guang Ji. Effects of Chinese herbal medicine Jiangzhi Granule on expressions of liver X receptor α and sterol regulatory element-binding protein-1c in a rat model of non-alcoholic fatty liver disease. Journal of Chinese Integrative Medicine, 2011, 9(9): 998-1004.
Full text



[1] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. An explanation on "guiding principles of clinical research on mild cognitive impairment (protocol)". Journal of Chinese Integrative Medicine, 2008, 6(1): 15-21
[2] Yi-ting He, Qing-lin Zha, Jian-ping Yu, Yong Tan, Cheng Lu, Ai-ping Lv. Principal factor analysis of symptoms of rheumatoid arthritis and their correlations with efficacy of traditional Chinese medicine and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 32-36
[3] Jun Cai, Hua Wang, Sheng Zhou, Bin Wu, Hua-rong Song, Zheng-rong Xuan. Effect of Sijunzi Decoction and enteral nutrition on T-cell subsets and nutritional status in patients with gastric cancer after operation: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(1): 37-40
[4] Wei Zhang, Xiang-feng Lu, Xiao-mei Zhang, Jian-jun Wu, Liang-duo Jiang. A rat model of pulmonary fibrosis induced by infusing bleomycin quickly through tracheal intubation. Journal of Chinese Integrative Medicine, 2008, 6(1): 60-67
[5] A-gao Zhou, Yong Zhang, Gang Kui, De-Yun Kong, Hai-liang Ge, Qiu-hua Ren, Jia-rong Dong, Sheng Hong, Xu-ming Mao, Yin Wang, Hui-zheng Zhang, Shu-jun Wang. Influence of traditional Chinese compound recipes with different efficacy on body weight, tumor weight and immune function in H22 cancer-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 77-82
[6] Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[7] Li Zhou, Hong-xing Zhang, Ling-guang Liu, Wen-jun Wan. Effect of electro-acupuncture at Fenglong (GV 16) on nitric oxide and endothelin in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 89-92
[8] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. Guiding principles of clinical research on mild cognitive impairment (protocol). Journal of Chinese Integrative Medicine, 2008, 6(1): 9-14
[9] Xin-jun Wang, Ling-ling Wang . A mechanism of endogenous opioid peptides for rapid onset of acupuncture effect in treatment of depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1014-1017
[10] Bo Wang , Wei Yan , Li-hui Hou, Xiao-ke Wu. Disorder of Tiangui (kidney essence) and reproductive dysfunction in patients with polycystic ovary syndrome. Journal of Chinese Integrative Medicine, 2010, 8(11): 1018-1022