Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (4): 299-302.doi: 10.3736/jcim20050415

• Original Experimental Research • Previous Articles     Next Articles

Effects of Anxin Granules on blood lipid and ultrastructure of aorta in rabbits with dyslipidemia

Xian-ming Fang1,Ying Yuan1,Yun-hong Wu1,Xue-man Yin2   

  1. 1. Department of Cardiovascular Internal Medicine, Ruikang Hospital, Guangxi College of Traditional Chinese Medicine, Nanning, Guangxi Province 530011, China
    2. Department of Pathology, Ruikang Hospital, Guangxi College of Traditional Chinese Medicine, Nanning, Guangxi Province 530011, China
  • Received:2004-11-08 Online:2005-07-20 Published:2005-07-20
  • Contact: Xian-ming Fang E-mail:fxm621@126.com

Objective

To study the effects of Anxin Granules on dyslipidemia in rabbits caused by high fat plus high cholesterol diet.

Methods

Thirty-two healthy New Zealand male white rabbits were randomly divided into 4 groups: normal control group, untreated group, Zhibituo Tablet-treated group and Anxin Granule-treated group. Rabbits in the normal control group were fed with regular chow, while rabbits in the other three groups were fed with high fat plus high cholesterol diet. Zhibituo Tablets and Anxin Granules were administered to the rabbits in Zhibituo Tablet-treated group and Anxin Granule-treated group at a daily oral dose respectively. At the end of the 10th week, the levels of serum total cholesterol (TC), triglycerides (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), apolipoprotein A1 (ApoA1) and apolipoprotein B (ApoB) were tested in each group, and the ultrastructures of the aorta were also observed by an electron microscope.

Results

Anxin Granules could reduce the levels of TC, TG, LDL-C and ApoB. The results observed by electron microscope showed that, as compared with the untreated group and the Zhibituo Tablet-treated group, the atherosclerosis of aorta in the Anxin Granule-treated group was lighter. And it was found that there were few lipid droplet vacuoles in cytoplasm of the endothelial cells, and various cell organs and elastic membrane were existed, but no lipid droplet vacuoles in cytoplasm of the medial smooth muscle cells.

Conclusion

Anxin Granules can regulate the metabolism of blood lipid and inhibit the formation of atherosclerosis caused by hyperlipidemia in rabbits.

Key words: Anxin Granules, Dyslipidemia, Models, Animal, Aorta, Ultrastructure

CLC Number: 

  • R589.21

Tab 1

Levels of TC, TG, HDL-C and LDL-C before and after administration in 4 groups ?$\bar{x}$±s, mmol/L)"

Group n TC TG HDL-C LDL-C
Normal control group
Before administration 8 1.55±0.41 1.39±0.42 0.61±0.09 0.35±0.30
After administration 8 1.52±0.79 0.74±0.07 0.51±0.09 0.68±0.75
Untreated group
Before administration 8 1.68±0.48 1.28±0.49 0.66±0.10 0.63±0.35
After administration 8 24.15±2.78** 2.42±1.03** 0.82±0.10 22.09±1.31**
Zhibituo Tablet group
Before administration 8 1.46±0.19 1.47±0.32 0.67±0.14 0.52±0.51
After administration 8 16.34±4.17△△ 0.74±0.22△△ 0.66±0.04 15.21±3.94△△
Anxin Granule group
Before administration 8 1.45±0.37 1.18±0.13 0.64±0.22 0.58±0.50
After administration 8 9.65±4.37△△▲▲ 0.83±0.19△△ 0.54±0.07 7.65±2.47△△▲▲

Tab 2

Levels of ApoA1, ApoB and ApoA1/ApoB before and after administration in 4 groups ($\bar{x}$±s)"

Group n ApoA1(mmol/L) ApoB (mmol/L) ApoA1/ApoB
Normal control group
Before administration 8 0.36±0.37 0.17±0.04 2.24±0.37
After administration 8 0.37±0.11 0.19±0.03 2.06±0.75
Untreated group
Before administration 8 0.41±0.32 0.22±0.19 2.26±0.35
After administration 8 0.96±0.22 1.34±0.10** 0.72±0.07**
Zhibituo Tablet group
Before administration 8 0.39±0.25 0.18±0.25 2.37±0.24
After administration 8 0.67±0.18 1.10±0.65 0.70±0.22
Anxin Granule group
Before administration 8 0.38±0.28 0.18±0.11 2.21±0.16
After administration 8 0.42±0.10 0.54±0.14△△ 0.80±0.15

Fig 1

Ultrastructure of rabbit aorta observed by electron microscope (1) A, B: Normal control group (×5000); C: Untreated group (×7500)"

Fig 2

Ultrastructure of rabbit aorta observed by electron microscope (2) A: Zhibituo Tablet-treated group (×7500); B, C: Anxin Granule-treated group (×10000)"

[1] 方显明, 肖柳华, 杨建设 . 益心脉颗粒治疗冠心病稳定型心绞痛30例[J]. 陕西中医, 2002,23(12):1092-1093
[2] 徐叔云, 卞如濂, 陈修 . 药理实验方法学(第3版)[M]. 北京: 人民卫生出版社, 2002,( 第3版):1202-1203
[3] 陈奇 . 中药药理实验方法学(第1版)[M]. 北京: 人民卫生出版社, 1993,( 第1版):1103-1105
[4] 侯旭敏, 倪唤春, 罗心平 , 等. 珍菊降压片对高脂血症兔内皮功能的改善作用[J]. 中西医结合学报, 2004,2(2):111-114
[5] 方云祥, 肖洲生 . 人参总皂甙对高脂饲养大鼠的脂质调节作用[J]. 湖南医科大学学报, 1995,20(5):425-426
[6] 李文雄, 曲丽芳, 冯雪梅 , 等. 中药复方对实验性老年高脂血症LPO, SOD和红细胞免疫功能的影响[J]. 成都中医药大学学报, 1997,20(3):48-50, 55
[7] 杨澄, 仇熙, 孔令东 . 炮制对桂枝抗氧化作用的影响[J]. 中成药, 2001,23(3):183-185
doi: 10.3969/j.issn.1001-1528.2001.03.011
[8] 李凤文, 刘晓颖, 张立石 , 等. 脉复康对高脂血症家兔血管内皮功能的影响[J]. 中国中医基础医学杂志, 2003,9(1):33-36
doi: 10.3969/j.issn.1006-3250.2003.01.013
[9] 方显明, 杨建设, 顾国龙 , 等. 益心脉颗粒对家兔心肌缺血再灌注心肌损伤保护作用的研究[J]. 中国中医药科技, 2003,10(2):78-80
[1] Lena Grönblom Lundström, Ulrika Aasa, Yan Zhang, Tobias Sundberg. Health care in light of different theories of health—A proposed framework for integrating a social humanistic perspective into health care. Journal of Integrative Medicine, 2019, 17(5): 321-327.
[2] Stefania Lamponi, Anna Maria Aloisi, Claudia Bonechi, Marco Consumi, Alessandro Donati, Gemma Leone, Claudio Rossi, Gabriella Tamasi, Luana Ghiandai, Ersilia Ferrini, Paolo Fiorenzani, Ilaria Ceccarelli, Agnese Magnani. Evaluation of in vitro cell and blood compatibility and in vivo analgesic activity of plant-derived dietary supplements. Journal of Integrative Medicine, 2019, 17(3): 213-220.
[3] Zahra Hajmohammadi, Mojtaba Heydari, Majid Nimrouzi, Pouya Faridi, Mohammad Javad Zibaeenezhad, Gholamhossein Ranjbar Omrani, Mesbah Shams . Rhus coriaria L. increases serum apolipoprotein-A1 and high-density lipoprotein cholesterol levels: A double-blind placebo-controlled randomized clinical trial. Journal of Integrative Medicine, 2018, 16(1): 45-50.
[4] Arthur Yin Fan, Sarah Faggert . Dr. Gene Bruno: The beginning of the acupuncture profession in the United States (1969-1979) — Acupuncture, medical acupuncture and animal acupuncture. Journal of Integrative Medicine, 2015, 13(5): 281-288.
[5] Vamsi Reddy, Arvind Sridhar, Roberto F. Machado, Jiwang Chen. High sodium causes hypertension: Evidence from clinical trials and animal experiments. Journal of Integrative Medicine, 2015, 13(1): 1-8.
[6] Zemin Yao​, Li Zhang​, Guang Ji​. Efficacy of polyphenolic ingredients of Chinese herbs in treating dyslipidemia of metabolic syndromes. Journal of Integrative Medicine, 2014, 12(3): 135-146.
[7] Zhao-lan Liu, Qing Li , Yu-jie Mu , Ying Gao, Jian-ping Liu. Using nested case-control study to appraise the effectiveness of Chinese medicines in the treatment of diabetic nephropathy. Journal of Chinese Integrative Medicine, 2012, 10(9): 991-996.
[8] Hong Wang , Jing-tian Ou , Xin Zhao , Ying Guoz , Hao-ping Mao. Vasodilator effect of oroxylin A on thoracic aorta isolated from rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 880-885.
[9] Zi-hui Li, Jian Wang, Rong-lin Cai, You-wen Wang, Jian-peng Hu. Establishment and evaluation of a rat model of ulcerative colitis with syndrome of dampness stagnancy due to spleen deficiency. Journal of Chinese Integrative Medicine, 2012, 10(8): 918-924.
[10] Chuan Chen, Hui-ying Chi, Zhi-hua Yu, Jiu-lin Chen . Effects of Chinese herbal medicine Shoushen Granule on telomere length and telomerase activity of peripheral white blood cells and vascular cells in rats with atherosclerosis. Journal of Chinese Integrative Medicine, 2012, 10(6): 667-673.
[11] Su-fang Zhang, Chang-quan Ling , Bai Li, Hong-yun Chen , Zhe Chen. Effects of Sisheng Decoction on the immunity and anti-stress function in mice with spleen deficiency syndrome. Journal of Chinese Integrative Medicine, 2012, 10(12): 1465-1469.
[12] You-hua Wang , Shang Li , Bin-rui Yang , Lun-qing Zhang , Xin Zhou , Bei-wen Xu, Ming-yuan Li . Use of zebrafish models for the research of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1189-1197.
[13] Hui Han , Li-min Wu , Ming-xiang Han, Wen-ming Yang , Dao-jun Xie , Zhao-hui Fang , Dan-chen Chu , Fang Fang . Effects of Chinese herbal medicine Shenxiong Yujing Granule on regeneration of neural cells in rats with diabetes-associated cerebral ischemia. Journal of Chinese Integrative Medicine, 2012, 10(10): 1140-1148.
[14] Liu Yue, Xie Ming, Zhang Ye. Dynamic changes of laboratory parameters of rats with type 2 diabetes and insulin resistance: defining their role in development of traditional Chinese medicine syndrome. Journal of Chinese Integrative Medicine, 2012, 10(1): 100-108.
[15] Qian Zhao, Rui Jin, Bing Zhang, Sen-mao Liu, Min Li, Xin Liu, Lian-zhen Li, Qian Zhang . Ridit analysis of experimental data from animal models of yang deficiency induced by different doses of hydrocortisone. Journal of Chinese Integrative Medicine, 2011, 9(9): 941-947.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[2] Wei Zhang, Xiang-feng Lu, Xiao-mei Zhang, Jian-jun Wu, Liang-duo Jiang. A rat model of pulmonary fibrosis induced by infusing bleomycin quickly through tracheal intubation. Journal of Chinese Integrative Medicine, 2008, 6(1): 60-67
[3] Li Zhou, Hong-xing Zhang, Ling-guang Liu, Wen-jun Wan. Effect of electro-acupuncture at Fenglong (GV 16) on nitric oxide and endothelin in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 89-92
[4] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[5] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[6] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[7] Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239
[8] Nian-hong Wang , Jun-tao Yan , Wu-quan Sun , Yong-shan Hu , Jun Xia , Li-cheng Wei , Jie Jia , Gui-lin Ouyang , Yong He , Yan-ming Guo , Jie Xu . Effects of early application of Tuina treatment on quadriceps surface myoelectricity in patients after total knee arthroplasty: a randomized controlled trial. Journal of Chinese Integrative Medicine, 2012, 10(11): 1247-1253
[9] Samadder Asmita, Das Jayeeta, Das Sreemanti, Biswas Raktim, Rahman Khuda-Bukhsh Anisur. Ameliorative potentials of Syzygium jambolanum extract against arsenic-induced stress in L6 cells in vitro. Journal of Chinese Integrative Medicine, 2012, 10(11): 1293-1302
[10] Peng-fei Gao, Kenji Watanabe. Introduction of the World Health Organization project of the International Classification of Traditional Medicine. Journal of Chinese Integrative Medicine, 2011, 9(11): 1161-1164