三拗汤类方对哮喘小鼠气道炎症反应的影响

doi:10.3736/jcim20090410

Abstract

Objective

To evaluate the effects of San'ao decoction (SAD) and its analogous prescriptions (APs), compounds of traditional Chinese herbal medicine for asthma, on airway inflammation in mice with respiratory syncytial virus (RSV)- and ovalbumin (OVA)-induced asthma.
Methods

A total of 110 mice were randomly divided into control group, untreated group, dexamethasone (DM) group, small-dose SAD (SAD-S) group, large-dose SAD (SAD-L) group, APⅠ-S group, APⅠ-L group, APⅡ-S group, APⅡ-L group, APⅢ-S group, and APⅢ-L group. The asthma model was reproduced by sensitization with multipoint intraperitoneal injection of OVA, followed by repeated inhalation of OVA combined with intranasal instillation of RSV. Cells in bronchoalveolar lavage fluid (BALF) were counted and classified. The supernatant of the BALF was used for detecting the contents of interleukin-4 (IL-4), interleukin-5 (IL-5) and interferon-gamma (IFN-γ) by enzyme-linked immunosorbent assay. Pathological changes in lung tissue were observed by hematoxilin and eosin staining and the scores of pathological changes were also calculated to determine the degree of inflammation.
Results

Compared with the control group, the amounts of lymphocytes, eosinophils, neutrophils in BALF in the untreated group were increased significantly (P<0.01); the changes of lung histopathology in the untreated group were much more serious, and the content of IFN-γ was sharply decreased, while the contents of IL-4 and IL-5 were significantly increased (P<0.05). The counts of eosinophils in BALF of the treated groups all decreased obviously (P<0.01) as compared with the untreated group. The count of the neutrophils in BALF of the APⅡ-L group was obviously lower than that in the untreated group (P<0.01). Most of Chinese herbal formulas and DM could increase the level of IFN-γ, and decrease the level of IL-4. All concentrations of the APs and SAD could decrease the level of IL-5 as compared with the untreated group, especially of the APⅡ-L and APⅠ-L (P<0.05, P<0.01).
Conslusion

SAD and its APs had some therapeutic effects on RSV-induced asthma in mice. Among the formulas, APⅡ has a better therapeutic efficacy in treatment of asthma by decreasing the amount of neutrophils.

Key words: Respiratory syncytial virus, Asthma, San'ao decoction, Neutrophils, Mice

Ying Zhang , Huang-jin Tong, Jing-hua Yu , Peng-cheng Gu, Xin-sheng Fan, Hui-qin Xu. Effects of San'ao decoction and its analogous prescriptions on airway inflammation in mice with respiratory syncytial virus- and ovalbumin-induced asthma. Journal of Chinese Integrative Medicine, 2009, 7(4): 354-359.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20090410

http://www.jcimjournal.com/EN/Y2009/V7/I4/354

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References 10

[1]	Tong HJ, Fan XS, Xu HQ, Wang L, Cui J . Reproduction of a type of viral asthma model in mice and its evaluation[J]. Xi’an Jiao Tong Da Xue Xue Bao Yi Xue Ban, 2008,29(3):349-352
	童黄锦, 范欣生, 许惠琴, 王露, 崔姣 . 一种病毒性哮喘模型制作方法的建立及评价[J]. 西安交通大学学报(医学版), 2008,29(3):349-352
[2]	Deng WW, Guo XJ. Allergic pulmonary disease[M]. Shanghai: Shanghai Scientific and Technical Publishers,2000: 58
	邓伟吾, 郭雪君 . 肺部变应性疾病[M]. 上海: 上海科学技术出版社,2000: 58
[3]	The summary of the sixth national conference on respiratory system of Chinese Medical Association[J]. Zhonghua Jie He He Hu Xi Za Zhi, 2001,24(1):8-11
	中华医学会第六次全国呼吸系学术会议纪要[J]. 中华结核和呼吸杂志, 2001,24(1):8-11
[4]	Lukacs NW, Lamm WJ, Strieter RM, Albert RK . Airway hyperreactivity is associated with specific leukocyte subset infiltration in a mouse model of allergic airway inflammation[J]. Pathobiology, 1996,64(6):308-313 doi: 10.1159/000164065
[5]	Anticevich SZ, Hughes JM, Black JL, Armour CL . Induction of hyperresponsiveness in human airway tissue by neutrophils mechanism of action[J]. Clin Exp Allergy, 1996,26(5):549-556 doi: 10.1111/cea.1996.26.issue-5
[6]	Park HS, Uh ST, Park CS . Increased neutrophil chemotactic activity is noted in alminum-induced occupational asthma[J]. Korean J Intern Med, 1996,11(1):69-73 doi: 10.3904/kjim.1996.11.1.69
[7]	Tumer MO, Hussack P, Sears MR, Dolovich J, Hargreave FE . Exacerbations of asthma without sputum eosinophilia[J]. Thorax, 1995,50(10):1057-1061 doi: 10.1136/thx.50.10.1057
[8]	The ENFUMOSA cross-sectional European multicentre study of the clinical phenotype of chronic severe asthma. European Network for Understanding Mecha-nisms of Severe Asthma[J]. Eur Respir J, 2003,22(3):470-477 doi: 10.1183/09031936.03.00261903
[9]	Jatakanon A, Uasuf C, Maziak W, Lim S, Chung KF, Barnes PJ . Neutrophillic inflammation in severe persistent asthma[J]. Am J Respir Crit Care Med, 1999,160(5 Pt 1):1532-1539 doi: 10.1164/ajrccm.160.5.9806170
[10]	Hussell T, Spender LC, Georgiou A, O'Garra A, Openshaw PJ .Th1 and Th2 cytokine induction in pulmonary T cells during infection with respiratory syncytial virus[J]. J Gen Virol, 1996,77(Pt 10):2447-2455 doi: 10.1099/0022-1317-77-10-2447

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Group	Dose (g/kg)	n	Cell count
Group	Dose (g/kg)	n	Macrophages	Lymphocytes	Eosinophils	Neutrophils
Control		10	10.14±2.30	2.23±1.00	0	0
Untreated		10	35.17±19.49	31.45±18.15**	3.83±1.24**	13.60±4.13**
DM	0.002 4	10	16.21±4.50	3.71±1.65	0.66±0.66^△△	9.45±8.31
SAD-S	4.4	10	11.33±2.92	5.06±0.65**^▲▲	0.34±0.18^△△	6.45±4.91
SAD-L	8.8	10	13.12±4.60	14.63±6.25*^▲	0.59±0.47^△△	6.70±3.85
APⅠ-S	7.4	10	6.55±2.07^□	4.92±2.19	0.34±0.29^△△	10.77±7.19
APⅠ-L	14.8	10	8.79±3.52	5.06±4.26	0.29±0.19^△△	9.67±4.68*
APⅡ-S	13.4	10	11.92±7.30	4.25±2.25	0.11±0.11^△△	6.75±5.22
APⅡ-L	26.8	10	13.95±7.17	0.73±0.64	0.42±0.50^△△	2.85±1.87^△△
APⅢ-S	4.8	10	12.75±8.99	5.68±1.51**^▲▲	0.60±0.65^△△	14.03±11.78
APⅢ-L	9.6	10	18.55±6.89	7.23±3.60^▲	0.58±0.51^△△	7.58±6.22

Group	Dose (g/kg)	n	IFN-γ	IL-4	IL-5
Control		10	30.08±4.01	12.11±1.51	15.25±4.07
Untreated		10	18.77±4.01*	23.30±12.04*	40.15±10.02*
DM	0.002 4	10	44.43±19.39	11.71±1.59	18.29±3.97
SAD-S	4.4	10	42.68±16.89	30.12±23.51	21.93±9.78
SAD-L	8.8	10	62.09±35.64	19.97±13.81	19.80±9.71
APⅠ-S	7.4	10	17.70±3.19*	16.04±14.09	26.87±18.93
APⅠ-L	14.8	10	66.43±20.10^△	14.72±10.02	7.66±3.20^△△
APⅡ-S	13.4	10	19.08±2.32*	12.27±2.60	19.02±1.64
APⅡ-L	26.8	10	29.02±11.60	11.57±0.62	9.05±2.32^△
APⅢ-S	4.8	10	59.20±24.30	21.39±14.48	21.67±16.70
APⅢ-L	9.6	10	65.72±38.40	19.77±15.62	14.69±8.98

Group	Dose (g/kg)	n	Pathological change score
Control		10	1.00±0.71
Untreated		10	5.00±1.27**
DM	0.002 4	10	2.00±0.79^△△
SAD-S	4.4	10	4.20±1.30
SAD-L	8.8	10	4.80±2.08
APⅠ-S	7.4	10	2.80±0.76^△
APⅠ-L	14.8	10	3.10±1.88
APⅡ-S	13.4	10	2.20±1.61^△△
APⅡ-L	26.8	10	2.50±0.71^△
APⅢ-S	4.8	10	2.70±1.20^△
APⅢ-L	9.6	10	4.20±2.51

Effects of San'ao decoction and its analogous prescriptions on airway inflammation in mice with respiratory syncytial virus- and ovalbumin-induced asthma

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