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Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (3): 196-198.doi: 10.3736/jcim20040313

• Original Experimental Research • Previous Articles     Next Articles

Effect of recipes replenishing qi and activating blood on cell proliferation and apoptosis in the liver of aging rats

Zhong-yi Yu1,Wei-kang Zhao2   

  1. 1. Pharmacological and Toxicological Research Center for Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203, China
    2. Geriatrics Institute,Shanghai University of Traditional Chinese Medicine,Shanghai 200032, China
  • Online:2004-06-20 Published:2018-10-25

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Objective: To observe the effect of recipes replenishing qi and activating blood on cell proliferation and apoptosis in the liver of natural aging rats.Methods: Natural aging rats were under administration of recipes replenishing qi or activating blood for 4 months. The liver of the rats was prepared into cell suspension for determination of cell proliferation and apoptosis with PI-staining and flow cytometer.Results: (1) Compared with those of the young rats, the cells in G0-G1 phase in the liver tissue of aging rats were increased (P<0.01), and apoptosis cells were increased (P<0.01), while the cells in S and G2-M phases were decreased(P<0.01). (2) Compared with those of the aging rats, the cells in G0-G1 phase in the liver tissue of aging rats administered recipes replenishing qi or activating blood were decreased (P<0.01), and it was more obvious in activating blood group than in replenishing qi group (P<0.01); the cells in S and G2-M phases were increased (P<0.01), and there was no significant difference between the activating blood group and the replenishing qi group (P>0.05). (3) The apoptosis cells in replenishing qi or activating blood group were decreased significantly (P<0.01), and the effect of replenishing qi was better than that of activating blood (P<0.01).Conclusion: (1) Cell proliferation is decreased and apoptosis is increased in the liver tissue of natural aging rats. (2) Recipes replenishing qi or activating blood can accelerate cell proliferation in the liver tissue of natural aging rats,and the effect of activating blood was slightly stronger than that of replenishing qi. (3) Recipes replenishing qi or activating blood can inhibit cell apoptosis in the liver tissue of natural aging rats,and the effect of replenishing qi was better than that of activating blood.

Key words: replenishing qi, activating blood, hepatocytes, aging, rats, laboratory

CLC Number: 

  • R242

Tab 1

Determination of cell proliferation and apoptosis in liver tissue of rats (ヌ±S,%)"

Group n Cells in G0-G1
phase		 Cells in S
phase		 Cells in G2-M
phase		 Cells in G2phase/
Cells in G1 phase		 apoptosis
cells
Aging control 6 67.7±9.0 8.0±6.8 24.2±12.7 1.78±0.34 9.29±4.68
Replenishing qi 6 62.2±12.9 5.3±1.5 34.5±12.2 1.98±0.07 6.58±2.01
Activating blood 6 58.5±7.9 5.7±3.9 35.7±4.7 2.01±0.03 7.39±5.66
Young control 6 49.9±13.3 8.3±2.5 41.8±15.7 2.03±1.78 6.97±1.22
[1] 童坦君, 张宗玉 . 医学老年学(第1版)[M]. 北京: 人民卫生出版社, 1995,( 第1版):12-16
[2] Cristofalo VJ, Volker C, Francis MK , et al. Age-dependent modifications of gene expression in human fibroblasts[J]. Crit Rev Eukaryot Gene Expr, 1998,8(1):43-80
doi: 10.1615/CritRevEukarGeneExpr.v8.i1.30 pmid: 9673450
[3] Shelton DN, Chang E, Whittier PS , et al. Microarray analysis of replicative senescence[J]. Curr Biol, 1999,9(17):939-945
doi: 10.1016/S0960-9822(99)80420-5 pmid: 10508581
[4] Cao SX, Dhahbi JM, Mote PL , et al. Genomic profiling of short- and long-term caloric restriction effects in the liver of aging mice[J]. Proc Natl Acad Sci U S A, 2001,98(19):10630-10635
[5] Lee CK, Klopp RG, Weindruch R , et al. Gene expression profile of aging and its retardation by caloric restriction[J]. Science, 1999,285(5432):1390-1393
doi: 10.1126/science.285.5432.1390 pmid: 10464095
[6] Prolla TA . DNA microarray analysis of the aging brain[J]. Chem Senses, 2002,27(3):299-306
doi: 10.1093/chemse/27.3.299 pmid: 11923192
[7] Weindruch R, Kayo T, Lee CK , et al. Microarray profiling of gene expression in aging and its alteration by caloric restriction in mice[J]. J Nutr, 2001,131(3):918S-923S
[8] Wang E, Lacelle C, Xu S , et al. Designer microarrays: from soup to nuts[J]. J Gerontol A Biol Sci Med Sci, 2002,57(11):B400-B405
doi: 10.1093/gerona/57.11.B400 pmid: 12403795
[9] 周文泉, 李祥国 . 实用中医老年病学(第1版)[M]. 北京: 人民卫生出版社,2000,(第1版):8- 11, 27-28
[10] 穆静, 李仪奎, 符胜光 . 参芪方血清对慢性肾衰大鼠肾脏细胞增殖的影响[J]. 中西医结合学报, 2003,1(2):122-124
Mu J, Li YK, Fu SG . Influence of Shenqi Recipe on mesangial cells and interstitial fibroblasts of chronic renal failure rats[J]. Zhong Xi Yi Jie He Xue Bao, 2003,1(2):122-124
[11] 郦章安, 吴春福 . 现代老年药学(第1版)[M]. 北京: 中国医药科技出版社, 2001,( 第1版):430-441
[12] 陈方敏, 赵伟康 . 补肾健脾益气活血法对衰老细胞增殖的调控作用[J]. 中药药理与临床, 2003,19(1):26-28
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