益气活血方药对衰老大鼠肝脏细胞增殖和凋亡的影响

doi:10.3736/jcim20040313

Abstract

Objective: To observe the effect of recipes replenishing qi and activating blood on cell proliferation and apoptosis in the liver of natural aging rats.Methods: Natural aging rats were under administration of recipes replenishing qi or activating blood for 4 months. The liver of the rats was prepared into cell suspension for determination of cell proliferation and apoptosis with PI-staining and flow cytometer.Results: (1) Compared with those of the young rats, the cells in G₀-G₁ phase in the liver tissue of aging rats were increased (P<0.01), and apoptosis cells were increased (P<0.01), while the cells in S and G₂-M phases were decreased(P<0.01). (2) Compared with those of the aging rats, the cells in G₀-G₁phase in the liver tissue of aging rats administered recipes replenishing qi or activating blood were decreased (P<0.01), and it was more obvious in activating blood group than in replenishing qi group (P<0.01); the cells in S and G₂-M phases were increased (P<0.01), and there was no significant difference between the activating blood group and the replenishing qi group (P>0.05). (3) The apoptosis cells in replenishing qi or activating blood group were decreased significantly (P<0.01), and the effect of replenishing qi was better than that of activating blood (P<0.01).Conclusion: (1) Cell proliferation is decreased and apoptosis is increased in the liver tissue of natural aging rats. (2) Recipes replenishing qi or activating blood can accelerate cell proliferation in the liver tissue of natural aging rats,and the effect of activating blood was slightly stronger than that of replenishing qi. (3) Recipes replenishing qi or activating blood can inhibit cell apoptosis in the liver tissue of natural aging rats,and the effect of replenishing qi was better than that of activating blood.

Key words: replenishing qi, activating blood, hepatocytes, aging, rats, laboratory

CLC Number:

R242

Zhong-yi Yu, Wei-kang Zhao. Effect of recipes replenishing qi and activating blood on cell proliferation and apoptosis in the liver of aging rats. Journal of Chinese Integrative Medicine, 2004, 2(3): 196-198.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20040313

http://www.jcimjournal.com/EN/Y2004/V2/I3/196

[1]	童坦君, 张宗玉 . 医学老年学(第1版)[M]. 北京: 人民卫生出版社, 1995,( 第1版):12-16
[2]	Cristofalo VJ, Volker C, Francis MK , et al. Age-dependent modifications of gene expression in human fibroblasts[J]. Crit Rev Eukaryot Gene Expr, 1998,8(1):43-80 doi: 10.1615/CritRevEukarGeneExpr.v8.i1.30 pmid: 9673450
[3]	Shelton DN, Chang E, Whittier PS , et al. Microarray analysis of replicative senescence[J]. Curr Biol, 1999,9(17):939-945 doi: 10.1016/S0960-9822(99)80420-5 pmid: 10508581
[4]	Cao SX, Dhahbi JM, Mote PL , et al. Genomic profiling of short- and long-term caloric restriction effects in the liver of aging mice[J]. Proc Natl Acad Sci U S A, 2001,98(19):10630-10635
[5]	Lee CK, Klopp RG, Weindruch R , et al. Gene expression profile of aging and its retardation by caloric restriction[J]. Science, 1999,285(5432):1390-1393 doi: 10.1126/science.285.5432.1390 pmid: 10464095
[6]	Prolla TA . DNA microarray analysis of the aging brain[J]. Chem Senses, 2002,27(3):299-306 doi: 10.1093/chemse/27.3.299 pmid: 11923192
[7]	Weindruch R, Kayo T, Lee CK , et al. Microarray profiling of gene expression in aging and its alteration by caloric restriction in mice[J]. J Nutr, 2001,131(3):918S-923S
[8]	Wang E, Lacelle C, Xu S , et al. Designer microarrays: from soup to nuts[J]. J Gerontol A Biol Sci Med Sci, 2002,57(11):B400-B405 doi: 10.1093/gerona/57.11.B400 pmid: 12403795
[9]	周文泉, 李祥国 . 实用中医老年病学(第1版)[M]. 北京: 人民卫生出版社,2000,(第1版):8- 11, 27-28
[10]	穆静, 李仪奎, 符胜光 . 参芪方血清对慢性肾衰大鼠肾脏细胞增殖的影响[J]. 中西医结合学报, 2003,1(2):122-124
	Mu J, Li YK, Fu SG . Influence of Shenqi Recipe on mesangial cells and interstitial fibroblasts of chronic renal failure rats[J]. Zhong Xi Yi Jie He Xue Bao, 2003,1(2):122-124
[11]	郦章安, 吴春福 . 现代老年药学(第1版)[M]. 北京: 中国医药科技出版社, 2001,( 第1版):430-441
[12]	陈方敏, 赵伟康 . 补肾健脾益气活血法对衰老细胞增殖的调控作用[J]. 中药药理与临床, 2003,19(1):26-28

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Group	n	Cells in G₀-G₁ phase	Cells in S phase	Cells in G₂-M phase	Cells in G₂phase/ Cells in G₁ phase	apoptosis cells
Aging control	6	67.7±9.0	8.0±6.8	24.2±12.7	1.78±0.34	9.29±4.68
Replenishing qi	6	62.2±12.9	5.3±1.5	34.5±12.2	1.98±0.07	6.58±2.01
Activating blood	6	58.5±7.9	5.7±3.9	35.7±4.7	2.01±0.03	7.39±5.66
Young control	6	49.9±13.3	8.3±2.5	41.8±15.7	2.03±1.78	6.97±1.22

Effect of recipes replenishing qi and activating blood on cell proliferation and apoptosis in the liver of aging rats

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