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Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (3): 196-198.doi: 10.3736/jcim20040313

• Original Experimental Research • Previous Articles     Next Articles

Effect of recipes replenishing qi and activating blood on cell proliferation and apoptosis in the liver of aging rats

Zhong-yi Yu1,Wei-kang Zhao2   

  1. 1. Pharmacological and Toxicological Research Center for Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203, China
    2. Geriatrics Institute,Shanghai University of Traditional Chinese Medicine,Shanghai 200032, China
  • Online:2004-06-20 Published:2018-10-25

Objective: To observe the effect of recipes replenishing qi and activating blood on cell proliferation and apoptosis in the liver of natural aging rats.Methods: Natural aging rats were under administration of recipes replenishing qi or activating blood for 4 months. The liver of the rats was prepared into cell suspension for determination of cell proliferation and apoptosis with PI-staining and flow cytometer.Results: (1) Compared with those of the young rats, the cells in G0-G1 phase in the liver tissue of aging rats were increased (P<0.01), and apoptosis cells were increased (P<0.01), while the cells in S and G2-M phases were decreased(P<0.01). (2) Compared with those of the aging rats, the cells in G0-G1 phase in the liver tissue of aging rats administered recipes replenishing qi or activating blood were decreased (P<0.01), and it was more obvious in activating blood group than in replenishing qi group (P<0.01); the cells in S and G2-M phases were increased (P<0.01), and there was no significant difference between the activating blood group and the replenishing qi group (P>0.05). (3) The apoptosis cells in replenishing qi or activating blood group were decreased significantly (P<0.01), and the effect of replenishing qi was better than that of activating blood (P<0.01).Conclusion: (1) Cell proliferation is decreased and apoptosis is increased in the liver tissue of natural aging rats. (2) Recipes replenishing qi or activating blood can accelerate cell proliferation in the liver tissue of natural aging rats,and the effect of activating blood was slightly stronger than that of replenishing qi. (3) Recipes replenishing qi or activating blood can inhibit cell apoptosis in the liver tissue of natural aging rats,and the effect of replenishing qi was better than that of activating blood.

Key words: replenishing qi, activating blood, hepatocytes, aging, rats, laboratory

CLC Number: 

  • R242

Tab 1

Determination of cell proliferation and apoptosis in liver tissue of rats (ヌ±S,%)"

Group n Cells in G0-G1
Cells in S
Cells in G2-M
Cells in G2phase/
Cells in G1 phase
Aging control 6 67.7±9.0 8.0±6.8 24.2±12.7 1.78±0.34 9.29±4.68
Replenishing qi 6 62.2±12.9 5.3±1.5 34.5±12.2 1.98±0.07 6.58±2.01
Activating blood 6 58.5±7.9 5.7±3.9 35.7±4.7 2.01±0.03 7.39±5.66
Young control 6 49.9±13.3 8.3±2.5 41.8±15.7 2.03±1.78 6.97±1.22
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