[an error occurred while processing this directive]

Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (1): 39-42.doi: 10.3736/jcim20060111

• Original Experimental Research • Previous Articles     Next Articles

Signal transduction mechanism of curcumin in inhibiting the proliferation of bovine lens epithelial cell induced by recombinant human epidermal growth factor

Yan-hong Hu1, Ming-xin Qi2, Xiu-rong Huang1, Lan Ma3, Jing Yan1, Zheng-zheng Wu1   

  1. 1. Research Center of Pathophysiology, Fujian College of Traditional Chinese Medicine, Fuzhou, Fujian Province 350003, China
    2. Department of Ophthalmology, Second People’s Hospital of Fujian Province, Fuzhou, Fujian Province 350003, China
    3. Department of Pathophysiology, Hainan Medical College, Haikou, Hainan Province 571101, China
  • Online:2006-01-20 Published:2006-01-15

RichHTML

3

PDF (PC)

109

Cited

16

Like

Objective

To investigate the signal transduction mechanism of curcumin in inhibiting the proliferation of bovine lens epithelial cell (LEC) induced by recombinant human epidermal growth factor (rhEGF).

Methods

There were three groups in this experiment, which were normal control group, untreated group and curcumin-treated group. Proliferation of LEC was induced by rhEGF (50 μg/L). The concentration of intracellular Ca2+ ([Ca2+]i) in LEC was measured with Fura-2/AM by spectrofluorimetry. The contents of intracellular cAMP and cGMP were assayed by radioimmunoassay.

Results

The [Ca2+]i in LEC was obviously increased in the untrated group as compared with that in the normal control group (P＜0.01), and the [Ca2+]i in LEC in the curcumin-treated group was highest among three groups (P＜0.01). The content of intracellular cAMP in LEC was decreased while the content of intracellular cGMP was obviously increased in the untreated group as compared with those in the normal control group (P＜0.01). The content of intracellular cAMP in LEC was higher in the curcumin-treated group than that in the untreated group, while the content of intracellular cGMP was lower than that in the untreated group (P＜0.01). 

Conclusion

The antiproliferation effects of curcumin on LEC may relate to the regulations of multiple processes of signal transduction.

Key words: Curcumin, Lens epithelial cell, Cell proliferation, Cataract, Signal transduction

CLC Number: 

  • R287.81

Tab 1

Effects of Cur on concentration of intracellular Ca2+ , cAMP and cGMP in bovine LEC undergoing proliferation induced by rhEGF（ヌ±S, nmol/L）"

Groupn[Ca2+]i in bovine LECcAMP concentrationcGMP concentration
Normal control6151.99±5.795.73±1.160.13±0.02
Untreated6305.98±53.75**3.28±1.26**0.20±0.05**
Cur-treated6398.02±13.85**△△5.49±2.07△△0.14±0.02△△
[1] Chauhan DP.Chemotherapeutic potential of curcumin for colorectal cancer[J].Curr Pharm Des,2002,8(19):1695-1706
[2] Choudhuri T, Pal S, Agwarwal ML, et al.Curcumin induces apoptosis in human breast cancer cells through p53-dependent Bax induction[J].FEBS Lett,2002,512(1-3):334-340
doi: 10.1016/S0014-5793(02)02292-5 pmid: 11852106
[3] Khar A, Ali AM, Pardhasaradhi BV, et al.Induction of stress response renders human tumor cell lines resistant to curcumin-mediated apoptosis: role of reactive oxygen intermediates[J].Cell Stress Chaperones,2001,6(4):368-376
[4] Anto RJ, Mukhopadhyay A, Denning K, et al.Curcumin(diferuloylmethane) induces apoptosis through activation of caspase-8, BID cleavage and cytochrome c release: its suppression by ectopic expression of Bcl-2 and Bcl-xl[J].Carcinogenesis,2002,23(1):143-150
[5] 卢建, 余应年, 徐仁宝.受体信号转导系统与疾病(第1版)[M].济南: 山东科学技术出版社,1999,(第1版):3-12
[6] 刘景生. 细胞信息与调控(第1版)[M].北京: 北京医科大学中国协和医科大学联合出版社,1999,(第1版):334
[7] 黄冬生, 陈金和, 吴基良.姜黄素诱导人肺癌细胞凋亡的实验研究[J].咸宁医学院学报,2002,16(4):252-255
doi: 10.3969/j.issn.1008-0635.2002.04.008
[1] Avijit Paul, Kausik Bishayee, Samrat Ghosh, Avinaba Mukherjee, Sourav Sikdar, Debrup Chakraborty, Naoual Boujedaini, Anisur Rahman Khuda-Bukhsh. Chelidonine isolated from ethanolic extract of Chelidonium majus promotes apoptosis in HeLa cells through p38-p53 and PI3K/AKT signalling pathways. Journal of Chinese Integrative Medicine, 2012, 10(9): 1025-1038.
[2] Dao-fang Ding , Song-pu Wei, Xiao-feng Li , Xiao-gang Zhang , Hong-sheng Zhan , Tie-li Duan , Yue-long Cao. Inhibition effect of osthole on proliferation of rat chondrocytes. Journal of Chinese Integrative Medicine, 2012, 10(12): 1413-1418.
[3] Mukherjee Avinaba, Sikdar Sourav, Bishayee Kausik, Paul Avijit, Ghosh Samrat, Boujedaini Naoual, Rahman Khuda-Bukhsh Anisur. Ethanolic extract of Thuja occidentalis blocks proliferation of A549 cells and induces apoptosis in vitro. Journal of Chinese Integrative Medicine, 2012, 10(12): 1451-1459.
[4] Li Zhang , Tao Wu , Jun-ming Chen , Li-li Yang , Hai-yan Song , Guang Ji. Danshensu inhibits acetaldehyde-induced proliferation and activation of hepatic stellate cell-T6. Journal of Chinese Integrative Medicine, 2012, 10(10): 1155-.
[5] Han Xiang-hui, Ye Yi-yi, Guo Bao-feng, Liu Sheng. Effects of platycodin D in combination with different active ingredients of Chinese herbs on proliferation and invasion of 4T1 and MDA-MB-231 breast cancer cell lines. Journal of Chinese Integrative Medicine, 2012, 10(1): 67-75.
[6] Bao Tan, Hai-lian Shi, Guang Ji, Lan Lu, Ai-li Cao, Song-shan Shi, Jian-qun Xie. Antiproliferative effects of essential oil of a compound Chinese herbal medicine Weiqi Decoction on AGS cells. Journal of Chinese Integrative Medicine, 2011, 9(5): 558-564.
[7] Zhao-hui Chu, Xiao-hua Liang, Xin-li Zhou, Ruo-fan Huang, Qiong Zhan, Jing-wei Jiang. Effects of deguelin on proliferation and apoptosis of MCF-7 breast cancer cells by phosphatidylinositol 3-kinase/Akt signaling pathway. Journal of Chinese Integrative Medicine, 2011, 9(5): 533-538.
[8] Bo Yang, Xue-chun Lu, Feng Zhang, Yang Liu, Hong-li Zhu. Inhibitory effects of traditional Chinese herbal medicine Tanreqing injection on proliferation of leukemia cells in vitro and the potential mechanisms. Journal of Chinese Integrative Medicine, 2011, 9(4): 414-422.
[9] Mei-na Ye, Hong-feng Chen, Rui-juan Zhou, Ming-juan Liao. Effects of Astragalus polysaccharide on proliferation and Akt phosphorylation of the basal-like breast cancer cell line. Journal of Chinese Integrative Medicine, 2011, 9(12): 1339-1346.
[10] Yang Chen, Jian-hua Huang, You Ning, Zi-yin Shen. Icariin and its pharmaceutical efficacy: Research progress of molecular mechanism. Journal of Chinese Integrative Medicine, 2011, 9(11): 1179-1184.
[11] Shou-fu Wang, Hui-jian Zhang, Jian-sheng Li, Jin-li Qin, Su-yun Li, Hai-bin Yu, Feng Wang, Ya Li, Si-hua Liu. Effects of Chinese herbal medicine Dusuqing Granule on toll-like receptor 4 signaling in multiple organ injury induced by bacterial pneumonia in aged rats. Journal of Chinese Integrative Medicine, 2011, 9(1): 84-90.
[12] Bing Hu, Hong-mei An, Ke-ping Shen, Qin Du. Effects of Tenglong Buzhong Decoction on proliferation and apoptosis of human colon carcinoma cell line LS174T. Journal of Chinese Integrative Medicine, 2010, 8(6): 575-580.
[13] Wei-yi Gong , Xin-min Zhang, Zi-yin Shen, Guo-rang Hu, Su-qin Zhang, Run-hong Liu, Wei-dong Zhang, Jing-cheng Dong. Optimal combination of baicalin, icariin and Astragalus saponin Ⅰ from a Chinese herbal compound Biminne. Journal of Chinese Integrative Medicine, 2010, 8(6): 541-547.
[14] Hai-bin Deng, Zhong-qi Wang , Zhen-ye Xu. Inhibitory effects of Feiyanning Decoction on proliferation, migration and tube formation of human umbilical vein endothelial cells in vitro. Journal of Chinese Integrative Medicine, 2009, 7(3): 255-260.
[15] Ying Deng , Hong-feng Chen. Effects of Astragalus injection and its ingredients on proliferation and Akt phosphorylation of breast cancer cell lines. Journal of Chinese Integrative Medicine, 2009, 7(12): 1174-1180.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[2] Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[3] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[4] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[5] Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[6] Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[7] Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[8] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[9] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[10] Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239
[an error occurred while processing this directive]