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Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (1): 39-42.doi: 10.3736/jcim20060111

• Original Experimental Research • Previous Articles     Next Articles

Signal transduction mechanism of curcumin in inhibiting the proliferation of bovine lens epithelial cell induced by recombinant human epidermal growth factor

Yan-hong Hu1, Ming-xin Qi2, Xiu-rong Huang1, Lan Ma3, Jing Yan1, Zheng-zheng Wu1   

  1. 1. Research Center of Pathophysiology, Fujian College of Traditional Chinese Medicine, Fuzhou, Fujian Province 350003, China
    2. Department of Ophthalmology, Second People’s Hospital of Fujian Province, Fuzhou, Fujian Province 350003, China
    3. Department of Pathophysiology, Hainan Medical College, Haikou, Hainan Province 571101, China
  • Online:2006-01-20 Published:2006-01-15

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Objective

To investigate the signal transduction mechanism of curcumin in inhibiting the proliferation of bovine lens epithelial cell (LEC) induced by recombinant human epidermal growth factor (rhEGF).

Methods

There were three groups in this experiment, which were normal control group, untreated group and curcumin-treated group. Proliferation of LEC was induced by rhEGF (50 μg/L). The concentration of intracellular Ca2+ ([Ca2+]i) in LEC was measured with Fura-2/AM by spectrofluorimetry. The contents of intracellular cAMP and cGMP were assayed by radioimmunoassay.

Results

The [Ca2+]i in LEC was obviously increased in the untrated group as compared with that in the normal control group (P＜0.01), and the [Ca2+]i in LEC in the curcumin-treated group was highest among three groups (P＜0.01). The content of intracellular cAMP in LEC was decreased while the content of intracellular cGMP was obviously increased in the untreated group as compared with those in the normal control group (P＜0.01). The content of intracellular cAMP in LEC was higher in the curcumin-treated group than that in the untreated group, while the content of intracellular cGMP was lower than that in the untreated group (P＜0.01). 

Conclusion

The antiproliferation effects of curcumin on LEC may relate to the regulations of multiple processes of signal transduction.

Key words: Curcumin, Lens epithelial cell, Cell proliferation, Cataract, Signal transduction

CLC Number: 

  • R287.81

Tab 1

Effects of Cur on concentration of intracellular Ca2+ , cAMP and cGMP in bovine LEC undergoing proliferation induced by rhEGF（ヌ±S, nmol/L）"

Groupn[Ca2+]i in bovine LECcAMP concentrationcGMP concentration
Normal control6151.99±5.795.73±1.160.13±0.02
Untreated6305.98±53.75**3.28±1.26**0.20±0.05**
Cur-treated6398.02±13.85**△△5.49±2.07△△0.14±0.02△△
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