[an error occurred while processing this directive]

Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (3): 302-306.doi: 10.3736/jcim20070314

• Original Experimental Research • Previous Articles     Next Articles

Effects of Jianpi Huoxue Decoction on Kupffer cell signal pathway activation in rats with liver injury induced by Lieber-Decarli liquid diet and lipopolysaccharide

Jing-hua Peng, Zhi-hong Fang, Jian-wei Cui, Qin Feng, Li-li Xu, Hong-tu Gu, Yi-yang Hu   

  1. Institute of Liver Diseases, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
  • Online:2007-05-31 Published:2007-05-15

RichHTML

7

PDF (PC)

351

Like

Objective: To observe the effects of Jianpi Huoxue Decoction, a compound Chinese herbal medicine, on Kupffer cell signal pathway activation in rats with liver injury induced by Lieber-Decarli liquid diet and lipopolysaccharide (LPS).Methods: SD rats were divided into normal, control liquid diet, ethanol liquid diet and ethanol liquid diet plus Jianpi Huoxue Decoction group. Rats were administrated with Jianpi Huoxue Decoction or distilled water via gastrogavage for 4 weeks after administration with ethanol or control liquid diet for 2 weeks respectively. After that, rats in each group were stimulated with LPS via gastrogavage for 3.5 h and harvested. Alanine aminotransferase (ALT) in serum and triglyceride (TG) in liver were analyzed. Pathological changes in liver tissues were observed in HE staining section. Tumor necrosis factor-α(TNF-α) in portal vein plasma was assayed by ELISA. The protein expressions of CD68, Toll-like receptor 4 (TLR4), phosphorylation-IκB (P-IκB) and TNF-α in liver were evaluated with Western-blotting.Result: After the treatment with Jianpi Huoxue Decoction, the pathologic changes in liver tissue were lightened, levels of ALT in serum, TG in liver and TNF-α in portal vein plasma were decreased, and the protein expressions of CD68, TLR4, P-IκB and TNF-α in liver were reduced.Conclusion: Jianpi Huoxue Decoction can inhibit Kupffer cell signal pathway activation in rats with liver injury induced by Lieber-Decarli liquid diet and LPS.

Key words: liver disease, alcoholic, endotoxin, lipopolysaccharides, tumor necrosis factor-α

CLC Number: 

  • R575

Figure 1

HE staining for liver tissue and immunohistochemical staining for CD68 (×200)A: HE staining for liver tissue; A1: Normal group; A2: Control liquid diet group; A3: Ethanol liquid diet group; A4: Jianpi Huoxue Decoction group. B: Immunohistochemical staining for CD68; B1: Normal group; B2: Control liquid diet group; B3: Ethanol liquid diet group; B4: Jianpi Huoxue Decoction group."

Table 1

Contents of TG in liver, TNF-α in portal vein plasma and activity of ALT in serum （ヌ±S）"

Group n TG in liver (mg/g) TNF-α in portal vein plasma (ng/L) ALT in serum (U/L)
Normal 6 38.93±16.51*△ 14.97±1.52** 39±4**
Control liquid diet 11 75.37±23.30* 15.91±1.19** 24±7**
Ethanol liquid diet 12 365.59±50.42 29.55±3.37 96±22
Jianpi Huoxue Decoction 12 245.59±63.85*△△ 26.00±2.77*△△ 75±18**△

Figure 2

Effects of Jianpi Huoxue Decoction on protein expressions of CD68, TLR4, P-IκB and TNF-α in rats with liver injury induced by Lieber-Decarli diet and LPS (Western-blotting)1: Normal group; 2: Control liquid diet group; 3: Ethanol liquid diet group; 4: Jianpi Huoxue Decoction group; *P＜0.05, *P＜0.01, vs ethanol liquid diet group."

[1] 庄辉 . 酒精性肝病的流行病学[J]. 中华肝脏病杂志, 2003,11(11):689
Zhuang H . Epidemiology of alcoholic liver disease[J]. Zhonghua Gan Zang Bing Za Zhi, 2003,11(11):689
[2] 胡义扬, 方志红, 崔剑巍 , 等. 酒精性肝损伤与小肠通透性改变的关系及中药的干预作用[J]. 科学技术与工程, 2005,5(22):1770-1771, 1776
Hu YY, Fang ZH, Cui JW , et al. Relationship between alcoholic liver injury and gut permeability and the alleviating effect of Chinese herbs[J]. Ke Xue Ji Shu Yu Gong Cheng, 2005,5(22):1770-1771, 1776
[3] Cui JW, Hu YY, Fang ZH , et al. Intervention effects of Jianpi Liqi Huoxue Decoction on lipid peroxidative liver injury induced by alcohol[J]. Chin J Integr Med, 2006,12(4):281-286
doi: 10.1007/s11655-006-0281-0
[4] Tsukamoto H, Lu SC . Current concepts in the pathogenesis of alcoholic liver injury[J]. FASEB J, 2001,15(8):1335-1349
[5] Thurman RG . Mechanisms of Hepatic Toxicity II[J]. Alcoholic liver injury involves activation of Kupffer cells by endotoxin. Am J Physiol Gastrointest Liver Physiol, 1998,275(4):G605-G611
[6] Adachi Y, Moore LE, Bradford BU , et al. Antibiotics prevent liver injury in rats following long-term exposure to ethanol[J]. Gastroenterology, 1995,108(1):218-224
[7] Hansen J, Cherwitz DL, Allen JI . The role of tumor necrosis factor-α in acute endotoxin-induced hepatotoxicity in ethanol-fed rats[J]. Hepatology, 1994,20(2):461-474
[8] Su GL . Lipopolysaccharides in liver injury: molecular mechanisms of Kupffer cell activation[J]. Am J Physiol Gastrointest Liver Physiol, 2002,283(2):256-265
doi: 10.1152/ajpgi.00550.2001 pmid: 12121871
[9] Uesugi T, Froh M, Arteel GE , et al. Toll-like receptor 4 is involved in the mechanism of early alcohol-induced liver injury in mice[J]. Hepatology, 2001,34(1):101-108
doi: 10.1053/jhep.2001.25350 pmid: 11431739
[1] Mao Zhi-min, Song Hai-yan, Yang Li-li, Liu Tao, Li Dong-fei, Zheng Pei-yong, Liu Ping, Ji Guang. Effects of the mixture of Swertia pseudochinensis Hara and Silybum marianum Gaertn extracts on CCl4-induced liver injury in rats with non-alcoholic fatty liver disease. Journal of Chinese Integrative Medicine, 2012, 10(2): 193-199.
[2] Qi-lin Fu, Yi-yang Hu, Qin Feng, Xiao-ning Wang, Jing-hua Peng, Tuan Cui. Analysis of major herbs in Chinese herbal formula Jianpi Huoxue Decoction for improving intestinal permeability based on uniform design. Journal of Chinese Integrative Medicine, 2011, 9(11): 1234-1241.
[3] Ping Xie, Xin-xi Zhou, Qin Zhang. Pathogenesis and treatment of non-alcoholic fatty liver disease. Journal of Chinese Integrative Medicine, 2010, 8(3): 201-209.
[4] Jun-ming Chen, Lei Wang, Tao Liu, Lian-jun Xing, Pei-yong Zheng, Guang Ji. Effects of Qinggan Huoxue Recipe and its separated recipes on urokinase-type plasminogen activator and plasminogen activator inhibitor-1 fibrinolytic system in rats with alcoholic liver fibrosis. Journal of Chinese Integrative Medicine, 2009, 7(7): 642-650.
[5] Hui Li , Shai-hui Du, Sai-xia Zhang, Ru-dong Deng, Chun Li, Yi-wei Li, Dong-feng Chen, Jian-hong Zhou . Effects of Niupo Zhibao Pellet on high-mobility group box-1 protein expression in lung tissues of endotoxin shock rats. Journal of Chinese Integrative Medicine, 2009, 7(5): 441-446.
[6] Arthur de Sa Ferreira, Januario Gomes Mouraoe Lima, Tatiana Pereira Teixeira Ferreira, Camila Madeira Tavares Lopes, Rubens Meyer. Prophylactic effects of short-term acupuncture on Zusanli (ST36) in Wistar rats with lipopolysaccharide-induced acute lung injury. Journal of Chinese Integrative Medicine, 2009, 7(10): 969-975.
[7] Hui Zhang, Qin Feng, Hong-shan Li, Shao-dong Chen, Xiao-ning Wang, Jing-hua Peng, Ning Zhang, Yi-yang Hu. Effects of Qushi Huayu Decoction on cathepsin B and tumor necrosis factor-α expression in rats with non-alcoholic steatohepatitis. Journal of Chinese Integrative Medicine, 2008, 6(9): 928-933.
[8] Yong-hong Li, Zong-wen Huang, Ping Xue, Jia Guo, Fu-qian He, Zheng You, Zheng-rong Wang. Effects of Chaiqin Chengqi Decoction on activation of nuclear factor-κB in pancreas of rats with acute necrotizing pancreatitis. Journal of Chinese Integrative Medicine, 2008, 6(2): 180-184.
[9] Tao Wu, Tao Liu, Pei-yong Zhang, Lian-jun Xing, Guang Ji. Effects of Qinggan Huoxue Recipe and its separated recipes on the expression of tumor necrosis factor-α in rats with alcoholic liver injury. Journal of Chinese Integrative Medicine, 2008, 6(11): 1145-1151.
[10] Hui Yang, Yi Li, Ling Cheng, Jin-sen He. Effect of electroacupuncture and diet adjusting on insulin resistance in rats with nutrition obesity. Journal of Chinese Integrative Medicine, 2007, 5(5): 546-549.
[11] Yi-yong Yang. Clinical observation of treatment of alcoholic fatty liver by Gehua Jiejiu Xiaozhi Decoction. Journal of Chinese Integrative Medicine, 2007, 5(3): 343-345.
[12] Jian-hong Zhou, Dong-feng Chen, Shao-hui Du, Hui Li, Yi-wei Li, Ru-dong Deng, Sai-xia Zhang. Effect of Niupo Zhibao Pellet on expression of neuronal nitric oxide synthase in brain of endotoxin-induced shock rats. Journal of Chinese Integrative Medicine, 2005, 3(2): 115-118.
[13] Xiao-yi Zhang, Cheng-bao Kuang. Clinical observation on treatment of nonalcoholic fatty liver disease complicating hyperuricemia by Zhifang Ⅰ Decoction. Journal of Chinese Integrative Medicine, 2004, 2(4): 265-267.
[14] Guo-jie Hu, Dian-gui Li, Shi-fang Li, Zhen-bin Li, Jun-xia Li. An animal model of alcoholic fatty liver and the prevention and treatment with New Qinggan Decoction. Journal of Chinese Integrative Medicine, 2004, 2(2): 123-125.
[15] Xiao-bin Mei, Wei-jie Yuan, Feng-lan Zhan, Hao Wu, Xiao-ying Zhang, Ruo-lan Cui. Role of cyclin kinase inhibitor p27 in inhibition of emodin on mesangial cell proliferation induced by tumor necrosis factor-α. Journal of Chinese Integrative Medicine, 2004, 2(2): 120-122.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[2] Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[3] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[4] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[5] Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[6] Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[7] Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[8] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[9] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[10] Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239
[an error occurred while processing this directive]