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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (5): 521-525.doi: 10.3736/jcim20070509

• Original Clinical Research • Previous Articles     Next Articles

Mechanism of salvianolate in preventing postoperative intestinal adhesion in rats

Xin-bin Sui1, Qing Zhang2, Hua-sen Qiu2,Ji-chun Zhou2,Xi-dong Gu1, Zhou-xiang Lu1, Chao Li1, Gang Wang1   

  1. 1. First Clinical Medical College,Zhejiang University of Traditional Chinese Medicine,Hangzhou,Zhejiang Province 310053,China
    2. Department of Gastrointestinal Surgery,Zhejiang Hospital of Traditional Chinese Medicine,Hangzhou,Zhejiang Province 310053,China
  • Online:2007-09-20 Published:2007-09-15

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Objective: To observe the effects of salvianolate on rats with postoperative intestinal adhesion and to explore the prevention mechanism.Methods: Forty SD male rats with intestinal adhesion were randomly divided into four groups: untreated group, low-dose salvianolate-treated group (12 mg/kg), medium-dose salvianolate-treated group (24 mg/kg) and high-dose salvianolate-treated group (48 mg/kg), with another ten SD male rats as normal control. Intraperitoneal injection of glucose was administered to the rats in the normal control group and the untreated group, and intraperitoneal injection of salvianolate was administered to the rats in the low-, medium- and high-dose salvianolate-treated groups. They were all treated for 8 days and once a day. On the eighth day after surgery the blood samples of each group were collected. Grades of intestinal adhesion were ranked by macroscopic observation. The adhesive tissues between viscera and belly wall were taken for pathological observation. The levels of interleukin-1β (IL-1β), interleukin-4 (IL-4) and tumor necrosis factor-α (TNF-α) were determined by enzyme linked immunosorbent assay.Results: Salvianolate can significantly reduce the extent of postoperative intestinal adhesion, obviously decrease the levels of IL-1β, TNF-α and inhibit the hyperplasy of fibrous connective tissue. However, there was no significant impact on the level of IL-4.Conclusion: Salvianolate can reduce the extent of postoperative intestinal adhesion, decrease the expression of IL-1β and TNF-α and inhibit the hyperplasy of fibrous connective tissue. This may be the mechanism of salvianolate in preventing intestinal adhesion.

CLC Number: 

  • R574

Table 1

Grades of intestinal adhesion in different groups"

Group n Grade of intestinal adhesion
0 Ⅰ Ⅱ Ⅲ Ⅳ
Normal control 10 8 2 0 0 0
Untreated* 10 0 1 0 7 2
Low-dose salvianolate-treated 10 3 2 1 2 2
Medium-dose salvianolate-treated△ 10 6 2 2 0 0
High-dose salvianolate-treated△ 10 7 3 0 0 0

Figure 1

Grades of intestinal adhesion in different groupsA: Normal control group (grade 0); B: Untreated group (grade Ⅲ); C: Untreated group (grade Ⅳ); D: Low-dose salvianolate-treated group (grade Ⅱ); E: Medium-dose salvianolate-treated group (grade Ⅰ); F: High-dose salvianolate-treated group (grade Ⅰ)."

Table 2

Levels of cytokines expressions in blood serum in different groups (ヌ±S, ng/L)"

Group n IL-1β IL-4 TNF-α
Normal control group 10 166.59±37.29 335.07±64.13 60.45±21.95
Untreated group 10 216.66±28.46* 315.00±40.77 119.69±50.37*
Low-dose salvianolate-treated 10 212.68±25.14 331.57±65.12 75.91±47.27△
Medium-dose salvianolate-treated 10 168.40±26.14△ 348.15±51.70 74.56±23.83△
High-dose salvianolate-treated 10 166.73±37.94△ 355.36±47.16 69.51±39.00△

Figure 2

Grades of tissue adhesion in different groups (HE staining, ×20) A: Normal control group (grade Ⅰ); B: Untreated group (grade Ⅲ); C: Low-dose salvianolate-treated group (grade Ⅱ); D: Medium-dose salvianolate-treated group (grade Ⅰ); E: High-dose salvianolate-treated group (grade Ⅰ)."

Table 3

Observation results of intestinal adhesion by HE staining in different groups"

Group n Ⅰ Ⅱ Ⅲ
Normal control group 10 9 1 0
Untreated group* 10 1 2 7
Low-dose salvianolate-treated△ 10 3 3 4
Medium-dose salvianolate-treated△ 10 6 3 1
High-dose salvianolate-treated△ 10 7 2 1
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