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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (5): 550-554.doi: 10.3736/jcim20070515

• Original Experimental Research • Previous Articles     Next Articles

Effects of Baobaole oral liquid on neuron excitability of feeding center in anorectic rats

Yue-ping Du1, Yue-ping Zhang2, Guo-cheng Zhang2   

  1. 1. Department of Traditional Chinese Medicine,Xijing Hospital,Fourth Military Medical University,Xi＇an,Shaanxi Province 710032,China
    2. Department of Pediatrics,Xijing Hospital,Fourth Military Medical University,Xi＇an,Shaanxi Province 710032,China
  • Online:2007-09-20 Published:2007-09-15

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Objective: To investigate the changes of neuropeptides content in a rat model of spleen-deficiency syndrome.Methods: Rats were randomly divided into three groups: normal group, untreated group and treatment group. There were ten rats in each group. Spleen-deficiency syndrome was induced by intragastrically administration of rhubarb concentrate in rats of the untreated and treatment group. The rats in the treatment group were intragastrically administered with Sijunzi decoction, a traditional Chinese compound herbal medicine for strengthening spleen and supporting qi. Normal saline was intragastrically administered to the rats in the normal and untreated groups. The somatostatin (SS) and cholecystokinin-8 (CCK-8) content in hypothalamus and colons was detected by the method of radioimmunoassay and the D-xylose content in urine was also detected.Results: The CCK-8 content in hypothalamus of the untreated group was higher than that of the normal group (P＜0.05). SS and CCK-8 content in colons of the untreated group was higher than that of the normal group (P＜0.05). The CCK-8 content in colons of the untreated group was lower than that of the treatment group (P＜0.05). The excretion rate of D-xylose in the untreated group was lower than that of the normal group (P＜0.05).Conclusion: The disorder of neuroendocrine regulation in rats with spleen-deficiency syndrome may be chiefly responsible for "spleen-deficency and dysfunction of spleen" in traditional Chinese medicine.

Key words: traditional Chinese herbs, anorexia, appetite regulation, lateral hypothalamic area, ventromedial

CLC Number: 

  • R442.1

Table 1

Effect of Baobaole oral liquid on daily food intake of anorectic juvenile rats (g, ヌ±S)"

GroupnFood intake at day 7Food intake at day 14Food intake at day 28
Normal1715.1±2.317.3±2.1△△20.8±2.5
Untreated1711.8±2.5*13.5±1.913.8±2.3
Baobaole-treated1712.5±2.2*16.6±2.3△20.1±3.0△△

Figure 1

Typical responses of LHA and VMN neurons arising from stimulating gastric vagus and intravenous injection of glucoseA: The excitation responses of a LHA neuron; B: The inhibition responses of another LHA neuron; C: The discharge rate of the neuron remarkably inhibited by intravenous injection of glucose; D: The inhibition responses of a VMN neuron; E: The excitation responses of another VMN neuron; F: The discharge rate of the neuron remarkably increased by intravenous injection of glucose"

Table 2

Responses of LHA neurons to gastric vagus stimulation"

GroupnNumber of LHA neurons
ExcitationInhibition
Normal control4013 (32.5%)27 (67.5%)
Untreated3210 (31.3%)22 (68.7%)
Baobaole-treated389 (23.7%)29 (76.3%)

Table 3

Responses of VMN neurons to gastric vagus stimulation"

GroupnNumber of VMN neurons
ExcitationInhibition
Normal control5039 (78.0%)11 (22.0%)
Untreated5846 (79.3% )12 (20.7%)
Baobaole-treated5544 (80.0%)11 (20.0%)

Table 3

Response characteristics of LHA neurons to gastric vagal stimulation in 3 groups (ヌ±S)"

GroupNeurons with excitation responseNeurons with inhibition response
nLatency(ms)Duration(ms)Stimulationintensity (μA)nLatency(ms)Duration(ms)Stimulationintensity (μA)

Table 5

Response characteristics of VMN neurons to gastric vagal stimulation in 3 groups (ヌ±S)"

GroupNeurons with excitation responseNeurons with inhibition response
nLatency(ms)Duration(ms)StimulationintensitynLatency(ms)Duration(ms)Stimulationintensity (μA)
Normal control39110.5±35.2223.3±76.1413.2±95.111123.5±55.2235.4±75.2420.0±74.8
Untreated46114.2±31.9278.3±88.6**△▲331.1±86.5**△△▲12119.2±41.9230.8±97.1436.4±61.5
Baobaole-treated44109.1±30.6235.5±84.9418.6±78.511129.1±50.6248.5±79.2430.0±78.1

Table 6

LHA and VMN neuron sensitivity to glucose in 3 groups (%)"

GroupNeurons responding to gastric vagal stimulationNeurons not responding to gastric vagal stimulation
Glycemia-sensitiveGlycemia-sensitiveGlycemia-sensitiveGlycemia-sensitive
neurons in LHAneurons in VMNneurons in LHAneurons in VMN
Normal control60.0 (18/30)33.3 (10/30)26.9 (7/26)28.6 (8/28)
Untreated25.0 (7/29)△63.3 (19/30)*△25.9 (7/27)32.1 (9/28)
Baobaole-treated64.5 (20/29)39.3 (11/28)33.3 (9/27)31.0 (9/29)
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doi: 10.1017/S0029665100000422 pmid: 10997653
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