“脾虚证”大鼠下丘脑及结肠组织生长抑素和胆囊收缩素-8含量的变化

doi:10.3736/jcim20070516

Abstract

Objective: To investigate the changes of neuropeptides content in a rat model of spleen-deficiency syndrome.Methods: Rats were randomly divided into three groups: normal group, untreated group and treatment group. There were ten rats in each group. Spleen-deficiency syndrome was induced by intragastrically administration of rhubarb concentrate in rats of the untreated and treatment group. The rats in the treatment group were intragastrically administered with Sijunzi decoction, a traditional Chinese compound herbal medicine for strengthening spleen and supporting qi. Normal saline was intragastrically administered to the rats in the normal and untreated groups. The somatostatin (SS) and cholecystokinin-8 (CCK-8) content in hypothalamus and colons was detected by the method of radioimmunoassay and the D-xylose content in urine was also detected.Results: The CCK-8 content in hypothalamus of the untreated group was higher than that of the normal group (P＜0.05). SS and CCK-8 content in colons of the untreated group was higher than that of the normal group (P＜0.05). The CCK-8 content in colons of the untreated group was lower than that of the treatment group (P＜0.05). The excretion rate of D-xylose in the untreated group was lower than that of the normal group (P＜0.05).Conclusion: The disorder of neuroendocrine regulation in rats with spleen-deficiency syndrome may be chiefly responsible for "spleen-deficency and dysfunction of spleen" in traditional Chinese medicine.

Key words: spleen asthenia, neuropeptides, hypothalamus, colon, rats

CLC Number:

R256.3

Qun Liu, Gan Cai. Content of somatostatin and cholecystokinin-8 in hypothalamus and colons in a rat model of spleen-deficiency syndrome. Journal of Chinese Integrative Medicine, 2007, 5(5): 555-558.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20070516

http://www.jcimjournal.com/EN/Y2007/V5/I5/555

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Group	n	Excretion rate of D-xylose (％)	Hypothalamus (pg/mg)		Mucous membrane of colon (pg/mg)
Group	n	Excretion rate of D-xylose (％)	SS	CCK-8	SS	CCK-8
Normal	10	26.06±4.66	34.44±9.68	12.70±1.40	7.17±3.95	6.33±2.20
Untreated	10	14.61±3.27*	35.93±12.59	16.04±3.38*	23.59±8.77*	4.01±1.53*
Treatment	10	22.51±4.07^△	31.63±9.08	15.44±2.78	18.10±15.67	7.04±2.52^△

Content of somatostatin and cholecystokinin-8 in hypothalamus and colons in a rat model of spleen-deficiency syndrome

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