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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (5): 555-558.doi: 10.3736/jcim20070516

• Original Experimental Research • Previous Articles     Next Articles

Content of somatostatin and cholecystokinin-8 in hypothalamus and colons in a rat model of spleen-deficiency syndrome

Qun Liu1, Gan Cai2   

  1. 1.Department of Traditional Chinese Medicine,Second Military Medical University,Shanghai 200433,China
    2.Department of Gastroenterology,Shuguang Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200021,China
  • Online:2007-09-20 Published:2007-09-15

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Objective: To investigate the changes of neuropeptides content in a rat model of spleen-deficiency syndrome.Methods: Rats were randomly divided into three groups: normal group, untreated group and treatment group. There were ten rats in each group. Spleen-deficiency syndrome was induced by intragastrically administration of rhubarb concentrate in rats of the untreated and treatment group. The rats in the treatment group were intragastrically administered with Sijunzi decoction, a traditional Chinese compound herbal medicine for strengthening spleen and supporting qi. Normal saline was intragastrically administered to the rats in the normal and untreated groups. The somatostatin (SS) and cholecystokinin-8 (CCK-8) content in hypothalamus and colons was detected by the method of radioimmunoassay and the D-xylose content in urine was also detected.Results: The CCK-8 content in hypothalamus of the untreated group was higher than that of the normal group (P＜0.05). SS and CCK-8 content in colons of the untreated group was higher than that of the normal group (P＜0.05). The CCK-8 content in colons of the untreated group was lower than that of the treatment group (P＜0.05). The excretion rate of D-xylose in the untreated group was lower than that of the normal group (P＜0.05).Conclusion: The disorder of neuroendocrine regulation in rats with spleen-deficiency syndrome may be chiefly responsible for "spleen-deficency and dysfunction of spleen" in traditional Chinese medicine.

Key words: spleen asthenia, neuropeptides, hypothalamus, colon, rats

CLC Number: 

  • R256.3

Table 1

SS, CCK-8 in hypothalamus and colons and D-xylose content in each group (ヌ±S)"

GroupnExcretion rate of D-xylose (％)Hypothalamus (pg/mg)Mucous membrane of colon (pg/mg)
SSCCK-8SSCCK-8
Normal1026.06±4.6634.44±9.6812.70±1.407.17±3.956.33±2.20
Untreated1014.61±3.27*35.93±12.5916.04±3.38*23.59±8.77*4.01±1.53*
Treatment1022.51±4.07△31.63±9.0815.44±2.7818.10±15.677.04±2.52△
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