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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (5): 555-558.doi: 10.3736/jcim20070516

• Original Experimental Research • Previous Articles     Next Articles

Content of somatostatin and cholecystokinin-8 in hypothalamus and colons in a rat model of spleen-deficiency syndrome

Qun Liu1, Gan Cai2   

  1. 1.Department of Traditional Chinese Medicine,Second Military Medical University,Shanghai 200433,China
    2.Department of Gastroenterology,Shuguang Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200021,China
  • Online:2007-09-20 Published:2007-09-15

Objective: To investigate the changes of neuropeptides content in a rat model of spleen-deficiency syndrome.Methods: Rats were randomly divided into three groups: normal group, untreated group and treatment group. There were ten rats in each group. Spleen-deficiency syndrome was induced by intragastrically administration of rhubarb concentrate in rats of the untreated and treatment group. The rats in the treatment group were intragastrically administered with Sijunzi decoction, a traditional Chinese compound herbal medicine for strengthening spleen and supporting qi. Normal saline was intragastrically administered to the rats in the normal and untreated groups. The somatostatin (SS) and cholecystokinin-8 (CCK-8) content in hypothalamus and colons was detected by the method of radioimmunoassay and the D-xylose content in urine was also detected.Results: The CCK-8 content in hypothalamus of the untreated group was higher than that of the normal group (P<0.05). SS and CCK-8 content in colons of the untreated group was higher than that of the normal group (P<0.05). The CCK-8 content in colons of the untreated group was lower than that of the treatment group (P<0.05). The excretion rate of D-xylose in the untreated group was lower than that of the normal group (P<0.05).Conclusion: The disorder of neuroendocrine regulation in rats with spleen-deficiency syndrome may be chiefly responsible for "spleen-deficency and dysfunction of spleen" in traditional Chinese medicine.

Key words: spleen asthenia, neuropeptides, hypothalamus, colon, rats

CLC Number: 

  • R256.3

Table 1

SS, CCK-8 in hypothalamus and colons and D-xylose content in each group (ヌ±S)"

GroupnExcretion rate of D-xylose (%)Hypothalamus (pg/mg)Mucous membrane of colon (pg/mg)
SSCCK-8SSCCK-8
Normal1026.06±4.6634.44±9.6812.70±1.407.17±3.956.33±2.20
Untreated1014.61±3.27*35.93±12.5916.04±3.38*23.59±8.77*4.01±1.53*
Treatment1022.51±4.0731.63±9.0815.44±2.7818.10±15.677.04±2.52
[1] 赵芹, 林爱华, 黄裕新, 等.功能性消化不良与MLT, NO, SP和SS的关系[J].中国现代医学杂志,2004,14(10):136-137
Zhao Q, Lin AH, Huang YX, et al.The correlation between functional dyspepsia and MLT, NO, SP and SS[J].Zhongguo Xian Dai Yi Xue Za Zhi,2004,14(10):136-137
[2] 任平, 黄熙, 李月彩, 等.脾虚证大鼠组织和血浆中SS、CCK、Mot含量变化[J].世界华人消化杂志,2000,8(4):436-438
Ren P, Huang X, Li YC, et al.Somatostatin, cholecystokinin and motilin levels in plasma and tissues in experimental Pixu rats[J].Shi Jie Hua Ren Xiao Hua Za Zhi,2000,8(4):436-438
[3] 胡琳琳, 高云芳, 何志仙.三种脾虚证模型小鼠消化吸收功能改变的比较研究[J].中国中西医结合杂志,2005,25(9):813-816
Hu LL, Gao YF, He ZX.Comparative study on changes of digestive and absorptive functions in three different models of pi-deficiency syndrome[J].Zhongguo Zhong Xi Yi Jie He Za Zhi,2005,25(9):813-816
[4] 封吉化, 尚虎虎, 黄熙, 等.脾虚早期与脾虚期大鼠组织和血浆中SS、CCK、MOT含量变化的探讨[J].武警医学,2005,16(5):336-339
Feng JH, Shang HH, Huang X, et al.Somatostatin, cholecystokinin and motilin levels in plasma and tissues in rats with spleen hypofunction at different stages[J].Wu Jing Yi Xue,2005,16(5):336-339
[5] 金益强, 刘璜英, 李学文.227例肝郁脾虚证患者小肠吸收功能障碍的分析[J].湖南医学院学报,1985,10(1):38-39
Jin YQ, Liu HY, Li XW.Analysis of 227 cases of dysfunction of small intestinal absorption in patients with stagnation of liver-qi and deficiency of spleen[J].Hunan Yi Xue Yuan Xue Bao,1985,10(1):38-39
[6] Binimelis J, Webb SM, Moné SJ, et al.Circulating immunoreactive somatostatin in gastrointestinal diseases[J].Scand J Gastroenterol,1987,22(8):931-937
[7] 桂先勇, 柯美云, 潘国宗, 等.肠易激综合征的结肠动力及胃肠激素变化[J].中华消化杂志,1994,14(增刊):50
Gu XY, Ke MY, Pan GZ, et al.Irritable bowel syndrome colon motility and gastrointestinal hormone changes[J].Zhonghua Xiao Hua Za Zhi,1994,14(suppl):50
[8] Hellstrm PM, Thollander M, Theodorsson E.Nociceptive inhibition of migrating myoelectric complex by nitric oxide and monoaminergic pathways in the rat[J].Am J Physiol,1998,274(3 pt 1):480-486
[9] 张宗英, 侯振江, 郭金英.胃肠激素及临床应用[J].世界华人消化杂志,1998,6(5):447-448
Zhang ZY, Hou ZJ, Guo JY.Gut hormones and clinical application[J].Shi Jie Hua Ren Xiao Hua Za Zhi,1998,6(5):447-448
[10] Reisine T, Bell GI.Molecular biology of somatostatin receptors[J].Endocr Rev,1995,16(4):427-442
doi: 10.1016/0166-2236(93)90050-V pmid: 7587653
[11] 朱金照, 陈东风, 冷恩仁.胃肠肽在胃肠运动调节中的作用[J].世界华人消化杂志,1999,7(8):687
Zhu JZ, Chen DF, Leng ER.The function of gastrointestinal peptide in regulation of gastrointestinal motility[J].Shi Jie Hua Ren Xiao Hua Za Zhi,1999,7(8):687
[12] 韩济生. 神经科学纲要[M].北京: 北京医科大学中国协和医科大学联合出版社,1993,():85
Han JS.The outline of neuroscience[M].Beijing: Publishing House of Beijing Medical University and Chinese Union Medical University,1993,():85
[13] Deschenes RJ, Haun RS, Funckes CL, et al.A gene encoding rat cholecystokinin: isolation, nucleotide sequence, and promoter activity[J].J Biol Chem,1985,260(2):1280-1286
doi: 10.1016/0165-022X(85)90070-3 pmid: 2981840
[14] 宋剑雄, 周吕, 柳力公, 等.下丘脑八肽胆囊收缩素对大鼠胃窦作用的影响[J].生理学报,1989,41(6):567-574
Song JX, Zhou L, Liu LG, et al.Influence of cholecystokinin-octopeptide in hypothalamus on sinus ventriculi in rats[J].Sheng Li Xue Bao,1989,41(6):567-574
[15] 魏睦新, 魏兰福, 邹百仓, 等.胃肠动力中药作用机制研究[J].中西医结合学报,2004,2(3):163-171
Wei MX, Wei LF, Zou BC, et al.Study on the mechanism of traditional Chinese medicines in promoting gastrointestinal peristalsis[J].Zhong Xi Yi Jie He Xue Bao,2004,2(3):163-171
[16] 方邦江, 朱培庭, 张静喆, 等.疏肝利胆中药防止胆固醇结石形成的实验研究[J].中西医结合学报,2006,4(1):56-59
Fang BJ, Zhu PT, Zhang JZ, et al.Inhibiting effects of recipe for dispersing stagnated liver qi to promote bile flow on cholesterol gallstone formation in guinea pigs[J].Zhong Xi Yi Jie He Xue Bao,2006,4(1):56-59
[17] Chey WY, Jin HO, Lee MH, et al.Colonic motility abnormality in patients with irritable bowel syndrome exhibiting abdominal pain and diarrhea[J].Am J Gastroenterol,2001,95(5):1499-1506
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