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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (1): 41-44.doi: 10.3736/jcim20080109

• Original Clinical Research • Previous Articles     Next Articles

Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis

Dong Yang1, Yong-ping Du1(), Qing Shen2, Wei Chen3, Yan Yu3, Guang-lei Chen3   

  1. 1. Department of Traditional Chinese Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, Shannxi Province 710032, China
    2. Department of Nephrology, Paediatrics Hospital, Fudan University, Shanghai 210000, China
    3. Department of Nephrology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shannxi Province 710032, China
  • Received:2007-05-15 Online:2008-01-20 Published:2008-01-15
  • Contact: DU Yong-ping E-mail:ddyypp@fmmu.edu.cn

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Objective:To investigate the expression of α-smooth muscle action (α-SMA) in the renal tubulointerstitium in patients with kidney collateral stasis.

Methods:The expression of α-SMA in the renal biopsy specimens from 54 patients with kidney collateral stasis was examined by immunohistochemical method.

Results:The degree of kidney collateral stasis was increased with the increasing of the degree of renal interstitial fibrosis (P<0.05), and there was significant positive correlation between kidney collateral stasis and α-SMA expression (P<0.05).

Conclusion:Kidney collateral stasis is one of the main reasons of renal fibrosis. With the increasing of kidney collateral stasis, MFBs in the renal interstitium proliferate obviously, becoming one of the most important causes of renal interstitial fibrosis.

Key words: nephrosis, fibrosis, alpha actin, fibroblasts

CLC Number: 

  • R692

Table 1

Index of blood stasis, expression of α-SMA in the renal tubulointerstitium and the content of serum creatinine and blood urea nitrogen in different groups (?x±s)"

Group n Index of blood stasis Expression of α-SMA Level of serum creatinine (μmol/L) Level of blood urea nitrogen (mmol/L)
Light fibrosis 22 3.14±0.56 9.10±5.48 74.81±18.9 5.03±1.55
Moderate fibrosis 16 4.87±0.89* 15.00±6.69* 107.40±42.98 7.32±3.56
Severe fibrosis 16 6.63±0.86*△ 31.58±7.16*△ 267.54±178.19*△ 12.38±6.03*△

Figure 1

Pathology characteristics of renal biopsy specimens under microscope (Masson staining, ×200) A: Light fibrosis; B: Moderate fibrosis; C: Severe fibrosis."

Figure 2

Expression of α-SMA in the renal tubulointerstitium under microscope (Immunohistochemical staining, ×200) Light fibrosis; B: Moderate fibrosis; C: Severe fibrosis."

[1] Li C. Organ fibrosis: foundation and clinic. Beijing: People’s Medical Publishing House. 2003: 113-114. Chinese.
李才 . 器官纤维化: 基础与临床.北京:人民卫生出版社. 2003: 113-114.
[2] China Society of Integrated Traditional Chinese and Western Medicine. Diagnostic criteria for syndrome of blood stasis. Zhong Xi Yi Jie He Za Zhi. 1987; 7(3):129. Chinese.
中国中西医结合研究会. 血瘀证诊断标准.中西医结合杂志. 1987; 7(3):129.
[3] Ning YY, Wang JQ , Qu SL. Effect of emodin on human kidney fibroblast proliferation. Zhong Xi Yi Jie He Za Zhi. Qu SL. Effect of emodin on human kidney fibroblast proliferation. Zhong Xi Yi Jie He Za Zhi. 2000; 20(2):105-106. Chinese with abstract in English.
doi: 10.3321/j.issn:1003-5370.2000.02.009
宁英远, 王俭勤, 屈遂林 . 大黄素对人肾成纤维细胞增殖的影响.中西医结合杂志. 2000; 20(2):105-106.
doi: 10.3321/j.issn:1003-5370.2000.02.009
[4] Gao JY , Shi ZS. Study of Dahuang Zhechong Pill in treating the rats with chronic renal function failure. Zhong- guo Zhong Yi Yao Ke Ji. 1998; 5(2):73-74. Chinese.
髙峻钰, 时振声 . 大黄蜜虫丸治疗大鼠慢性肾功能衰竭的实验研究.中国中医药科技. 1998; 5(2):73-74.
[5] Hu HX, Liu ZM, Cai Q , et al. Effect of Yishen Huoxue Xiezhuo method on TGF-p in the nephridial tissue of the rats with renal function failure. Zhongguo Zhong Yi Ji Chu Yi Xue Za Zhi. 2000; 6(6):369-371. Chinese.
胡海翔, 刘占民, 蔡庆 , 等. 益肾活血泻浊方对慢性肾衰大鼠肾组织TGF-p的影响.中国中医基础医学杂志. 2000; 6(6):369-371.
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