Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (1): 41-44.doi: 10.3736/jcim20080109

• Original Clinical Research • Previous Articles     Next Articles

Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis

Dong Yang1, Yong-ping Du1(), Qing Shen2, Wei Chen3, Yan Yu3, Guang-lei Chen3   

  1. 1. Department of Traditional Chinese Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, Shannxi Province 710032, China
    2. Department of Nephrology, Paediatrics Hospital, Fudan University, Shanghai 210000, China
    3. Department of Nephrology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shannxi Province 710032, China
  • Received:2007-05-15 Online:2008-01-20 Published:2008-01-15
  • Contact: DU Yong-ping E-mail:ddyypp@fmmu.edu.cn

Objective:To investigate the expression of α-smooth muscle action (α-SMA) in the renal tubulointerstitium in patients with kidney collateral stasis.

Methods:The expression of α-SMA in the renal biopsy specimens from 54 patients with kidney collateral stasis was examined by immunohistochemical method.

Results:The degree of kidney collateral stasis was increased with the increasing of the degree of renal interstitial fibrosis (P<0.05), and there was significant positive correlation between kidney collateral stasis and α-SMA expression (P<0.05).

Conclusion:Kidney collateral stasis is one of the main reasons of renal fibrosis. With the increasing of kidney collateral stasis, MFBs in the renal interstitium proliferate obviously, becoming one of the most important causes of renal interstitial fibrosis.

Key words: nephrosis, fibrosis, alpha actin, fibroblasts

CLC Number: 

  • R692

Table 1

Index of blood stasis, expression of α-SMA in the renal tubulointerstitium and the content of serum creatinine and blood urea nitrogen in different groups (?x±s)"

Group n Index of blood stasis Expression of α-SMA Level of serum creatinine (μmol/L) Level of blood urea nitrogen (mmol/L)
Light fibrosis 22 3.14±0.56 9.10±5.48 74.81±18.9 5.03±1.55
Moderate fibrosis 16 4.87±0.89* 15.00±6.69* 107.40±42.98 7.32±3.56
Severe fibrosis 16 6.63±0.86*△ 31.58±7.16*△ 267.54±178.19*△ 12.38±6.03*△

Figure 1

Pathology characteristics of renal biopsy specimens under microscope (Masson staining, ×200) A: Light fibrosis; B: Moderate fibrosis; C: Severe fibrosis."

Figure 2

Expression of α-SMA in the renal tubulointerstitium under microscope (Immunohistochemical staining, ×200) Light fibrosis; B: Moderate fibrosis; C: Severe fibrosis."

[1] Li C. Organ fibrosis: foundation and clinic. Beijing: People’s Medical Publishing House. 2003: 113-114. Chinese.
李才 . 器官纤维化: 基础与临床.北京:人民卫生出版社. 2003: 113-114.
[2] China Society of Integrated Traditional Chinese and Western Medicine. Diagnostic criteria for syndrome of blood stasis. Zhong Xi Yi Jie He Za Zhi. 1987; 7(3):129. Chinese.
中国中西医结合研究会. 血瘀证诊断标准.中西医结合杂志. 1987; 7(3):129.
[3] Ning YY, Wang JQ , Qu SL. Effect of emodin on human kidney fibroblast proliferation. Zhong Xi Yi Jie He Za Zhi. Qu SL. Effect of emodin on human kidney fibroblast proliferation. Zhong Xi Yi Jie He Za Zhi. 2000; 20(2):105-106. Chinese with abstract in English.
doi: 10.3321/j.issn:1003-5370.2000.02.009
宁英远, 王俭勤, 屈遂林 . 大黄素对人肾成纤维细胞增殖的影响.中西医结合杂志. 2000; 20(2):105-106.
doi: 10.3321/j.issn:1003-5370.2000.02.009
[4] Gao JY , Shi ZS. Study of Dahuang Zhechong Pill in treating the rats with chronic renal function failure. Zhong- guo Zhong Yi Yao Ke Ji. 1998; 5(2):73-74. Chinese.
髙峻钰, 时振声 . 大黄蜜虫丸治疗大鼠慢性肾功能衰竭的实验研究.中国中医药科技. 1998; 5(2):73-74.
[5] Hu HX, Liu ZM, Cai Q , et al. Effect of Yishen Huoxue Xiezhuo method on TGF-p in the nephridial tissue of the rats with renal function failure. Zhongguo Zhong Yi Ji Chu Yi Xue Za Zhi. 2000; 6(6):369-371. Chinese.
胡海翔, 刘占民, 蔡庆 , 等. 益肾活血泻浊方对慢性肾衰大鼠肾组织TGF-p的影响.中国中医基础医学杂志. 2000; 6(6):369-371.
[1] Na Zhi, Qian Mo, Shuo Yang, Yuan-xing Qin, Hao Chen, Zeng-guang Wu, Cai-hong Lan, Jun Zhang, Yin-long Li. Treatment of pulmonary fibrosis in one convalescent patient with corona virus disease 2019 by oral traditional Chinese medicine decoction: A case report. Journal of Integrative Medicine, 2021, 19(2): 185-190.
[2] Qing-lan Wang, Yan-yan Tao, Hong-dong Xie, Cheng-hai Liu, Ping Liu. Fuzheng Huayu recipe, a traditional Chinese compound herbal medicine, attenuates renal interstitial fibrosis via targeting the miR-21/PTEN/AKT axis. Journal of Integrative Medicine, 2020, 18(6): 505-513.
[3] Hepatology Committee of Chinese Association of Integrative Medicine, China. Guidelines for diagnosis and treatment of hepatic fibrosis with integrated traditional Chinese and Western medicine (2019 edition). Journal of Integrative Medicine, 2020, 18(3): 203-213.
[4] Xue-qing Yu, Shu-guang Yang, Yang Xie, Jian-sheng Li. Traditional Chinese medicine in the treatment of idiopathic pulmonary fibrosis based on syndrome differentiation: Study protocol of exploratory trial. Journal of Integrative Medicine, 2020, 18(2): 163-168.
[5] Stefania Lamponi, Anna Maria Aloisi, Claudia Bonechi, Marco Consumi, Alessandro Donati, Gemma Leone, Claudio Rossi, Gabriella Tamasi, Luana Ghiandai, Ersilia Ferrini, Paolo Fiorenzani, Ilaria Ceccarelli, Agnese Magnani. Evaluation of in vitro cell and blood compatibility and in vivo analgesic activity of plant-derived dietary supplements. Journal of Integrative Medicine, 2019, 17(3): 213-220.
[6] Hoda Mojiri-Forushani, Ali Asghar Hemmati, Mohammad Amin Dehghani, Ali Reza Malayeri, Hossein Hassan Pour. Effects of herbal extracts and compounds and pharmacological agents on pulmonary fibrosis in animal models: A review. Journal of Integrative Medicine, 2017, 15(6): 433-441.
[7] JiaXia, Li-qunHe, XiaoSu. Interventional mechanisms of herbs or herbal extracts on renal interstitial fibrosis. Journal of Integrative Medicine, 2016, 14(3): 165-173.
[8] Takanobu Takata, Yoshiharu Motoo, Naohisa Tomosugi. Effect of Saikokeishito, a Kampo medicine, on hydrogen peroxide-induced premature senescence of normal human dermal fibroblasts. Journal of Integrative Medicine, 2014, 12(6): 495-503.
[9] Shu Dong, Shi-bing Su​. Advances in mesenchymal stem cells combined with traditional Chinese medicine therapy for liver fibrosis. Journal of Integrative Medicine, 2014, 12(3): 147-155.
[10] Qing-lan Wang, Yan-yan Tao , Li Shen , Hong-yan Cui , Cheng-hai Liu. Chinese herbal medicine Fuzheng Huayu recipe inhibits liver fibrosis by mediating the transforming growth factor-β1/Smads signaling pathway. Journal of Chinese Integrative Medicine, 2012, 10(5): 561-568.
[11] Zhou Xian-mei, Zhao Yang, He Cui-cui, Li Jian-xin. Preventive effects of Citrus reticulata essential oil on bleomycin-induced pulmonary fibrosis in rats and the mechanism. Journal of Chinese Integrative Medicine, 2012, 10(2): 200-209.
[12] Xiao-ning Wang, Qing Tao, Qin Feng, Jing-hua Peng, Ping Liu, Yi-yang Hu. Effects of Chinese herbal medicine Yiguanjian Decoction on collagen metabolism of hepatic tissues in rats with CCI4-induced liver fibrosis. Journal of Chinese Integrative Medicine, 2011, 9(6): 651-657.
[13] Hua-qiang Zhai, Shuo-feng Zhang, Ming-chao Gao, Yang Liu, Min Ou, Fan-yun Meng, Yong-yan Wang. Effects of Herba Ephedra Sinicae and Fructus Schisandrae Chinensis on pathology of rats with bleomycin A5-induced idiopathic pulmonary fibrosis. Journal of Chinese Integrative Medicine, 2011, 9(5): 553-557.
[14] Wen Sun, Zhi Zhu, Jian Yu, Yong-hong Wang, Man Xiong, Xia Gao, Zhong-hua Zhao, Xue-guang Liu. Effects of Chinese herbal medicine Huaiqihuang Granule on nephrin and podocin expressions in renal tissues of rats with adriamycin-induced nephrosis. Journal of Chinese Integrative Medicine, 2011, 9(5): 546-552.
[15] Yan Shen, Hong Wei, Chun-lan Jin. Effects of Chinese herbal medicine Xuefu Zhuyu Decoction on angiotensin Ⅱ-induced proliferation and extracellular matrix synthesis of rat cardiac fibroblasts. Journal of Chinese Integrative Medicine, 2011, 9(3): 313-319.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[2] Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[3] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[4] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[5] Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[6] Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[7] Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[8] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[9] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[10] Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239