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Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (3): 313-319.doi: 10.3736/jcim20110313

• Original Experimental Research • Previous Articles     Next Articles

Effects of Chinese herbal medicine Xuefu Zhuyu Decoction on angiotensin Ⅱ-induced proliferation and extracellular matrix synthesis of rat cardiac fibroblasts

Yan Shen(), Hong Wei, Chun-lan Jin   

  1. Department of Cardiology, Yueyang Hospital of Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
  • Received:2010-12-13 Accepted:2011-01-04 Online:2011-03-20 Published:2011-03-15
  • Contact: Shen Yan E-mail:sheny26@126.com

Objective: To investigate the effects of Xuefu Zhuyu Decoction, a compound Chinese herbal medicine, on the proliferation and extracellular matrix synthesis of cardiac fibroblasts (CFs) from neonatal rats induced by angiotensin Ⅱ, and to explore the mechanism of Xuefu Zhuyu Decoction in improving myocardial fibrosis.
Methods: CFs derived from neonatal rats were cultured with enzymatic dissociation to establish a cell model of cardiac fibrosis induced by angiotensin Ⅱ in vitro. CFs were divided into 6 groups, including blank control group, angiotensin Ⅱ group, valsartan-medicated serum group, and 5%, 10% and 15% Xuefu Zhuyu decoction-medicated serum group. CFs in the blank control group were incubated with normal rat serum; CFs in the other groups were stimulated with angiotensin Ⅱ at a dose of 10 –6 mol/L, and then incubated with normal rat serum and 5%, 10% and 15% Xuefu Zhuyu decoction-medicated serum for 24 h, respectively. Methyl thiazolyl tetrazolium assay was employed to measure CF proliferation, and collagen synthesis was detected by analysis of the hydroxyproline in CFs. Radioimmunoassay was used to measure concentrations of procollagen Ⅲ and hyaluronic acid. Enzyme-linked immunosorbent assay was used to measure fibronectin expression.
Results: Compared with the blank control group, angiotensin Ⅱ induced cardiac fibroblast proliferation, stimulated DNA and collagen syntheses (P<0.01), and increased fibronectin, hyaluronic acid and procollagen Ⅲ concentrations (P<0.01). Xuefu Zhuyu decoction-medicated serum inhibited CF proliferation and DNA and collagen syntheses (P<0.05), and decreased the concentrations of fibroectin, hyaluronic acid and procollagen Ⅲ (P<0.01).
Conclusion: Angiotensin Ⅱ can induce cardiac fibrosis, stimulate cardiac fibroblast proliferation and extracellular matrix synthesis, while Xuefu Zhuyu Decoction can prevent cardiac fibrosis, the mechanism of which may be related to inhibiting CF proliferation and extracellular matrix synthesis.

Key words: cardiac fibrosis, cardiac remodeling, drugs,Chinese herbal, angiotensin H, extracellular matrix

Figure 1

Morphology and identification of cardiac fibroblasts A: Fibroblasts were fusiform and polygonal and observed by hematoxylin and eosin staining under an inverted microscope (×100); B: Fibroblasts were negative for α-smooth muscle actin and observed by EnVision under an inverted microscope (×200); C: Fibroblasts were positive for fibroectin expression and observed by EnVision method under an inverted microscope (×200); D: Fibroblasts were positive for vitronectin and observed by EnVision method under an inverted microscope (×200)."

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