[an error occurred while processing this directive]

Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (3): 263-268.doi: 10.3736/jcim20100311

• Original Experimental Research • Previous Articles     Next Articles

Effects of Shenqi Jiedu Decoction on expressions of transforming growth factor-β1, smad2 and smad3 in renal tissues of rats with chronic renal failure induced by adenine

 Ting-ting Lia, Guo-min Sia, Feng-cui Chenb   

  1. a Department of Traditional Chinese Medicine, Shandong Provincial Hospital, Shandong University, Jinan 250021, Shandong Province, China
    b Department of Pediatrics, Rushan Hospital of Traditional Chinese Medicine, Rushan 264500, Shandong Province, China
  • Received:2009-11-09 Accepted:2010-01-10 Online:2010-03-20 Published:2010-03-15
  • Contact: Guo-min Si E-mail:sgm977@126.com

RichHTML

3

PDF (PC)

73

Like

Objective

To investigate the effects of Shenqi Jiedu Decoction (SQJDD), a compound traditional Chinese herbal medicine, on expressions of transforming growth factor beta1 (TGF-β1), smad2 and smad3 proteins in renal tissues of rats with chronic renal failure (CRF) induced by adenine, and to explore the mechanisms of SQJDD in treating CRF.
Methods

Ninety healthy Wistar male rats were randomly divided into six groups: normal group, untreated group, losartan group, low-dose SQJDD group, medium-dose SQJDD group, and high-dose SQJDD group. Rat model of CRF was established by feeding 2.5% adenine. Levels of plasma creatinine (Cr) and blood urea nitrogen (BUN) in different groups after model establishment were detected to estimate the renal function of rats with CRF. After eight-week treatment, levels of Cr, BUN, triacylglycerol (TAG), cholesterol (Chol) and albumin (ALB) in plasma were detected. Pathological changes of renal tissues were observed by HE staining. Protein expressions of TGF-β1, Smad2 and Smad3 in renal tissues were detected by Western blotting.
Results

Compared with pretreatment, levels of Cr and BUN in the losartan group and the SQJDD groups were markedly decreased (P<0.01). After treatment, levels of Cr, BUN and Chol in all treated groups were lower than those in the untreated group (P<0.01, P<0.05). Levels of ALB in all treated groups were higher than that in the untreated group (P<0.01). Pathological lesions of renal tissues in all treated groups were lessened as compared with the untreated group (P<0.01). The Western blotting results showed that the protein expressions of TGF-β1, Smad2 and Smad3 in renal tissues in all treated groups were lower than those in the untreated group (P<0.01).
Conclusion

SQJDD can delay the progress of CRF by inhibiting TGF-β1/Smads signal transduction pathway.

Key words: Chronic renal failure, Compound (TCD), Transforming growth factor betal, Smad2, Smad3, Rats

Table 1

Plasma Cr and BUN levels of rats in different groups after model establishment and treatment (x±s)"

Group n Cr (μmol/L) BUN (mmol/L)
Before treatment After treatment Before treatment After treatment
Normal 15 50.62±10.04 50.45±8.55 8.05±1.10 8.20±0.85
Untreated 14 123.62±18.37** 122.10±17.40** 47.89±4.06** 45.41±3.62**
Losartan 13 118.58±15.14** 90.10±2.90**△△▲▲ 48.32±3.89** 30.82±1.55**△△▲▲
Low-dose SQJDD 13 122.40±18.48** 97.72±5.07**△△▲▲ 45.79±3.59** 30.90±3.91**△△▲▲
Medium-dose SQJDD 15 119.78±14.23** 93.09±5.55**△△▲▲ 48.53±3.92** 32.76±2.08**△△▲▲
High-dose SQJDD 14 120.43±14.50** 90.53±3.00**△△▲▲ 46.06±2.33** 29.81±4.04**△△▲▲

Table 2

Plasma TAG, Chol and ALB levels of rats in different groups after treatment (x±s)"

Group n TAG (mmol/L) Chol (mmol/L) ALB (g/L)
Normal 15 0.73±0.10 1.04±0.11 30.53±1.30
Untreated 14 1.25±0.10** 1.90±0.19** 22.80±1.82**
Losartan 13 1.07±0.16** 1.68±0.18**△ 27.89±1.52**△△
Low-dose SQJDD 13 1.14±0.26** 1.75±0.23**△ 26.40±1.65**△△
Medium-dose SQJDD 15 1.11±0.25** 1.70±0.21**△ 27.09±1.16**△△
High-dose SQJDD 14 1.05±0.13** 1.68±0.16**△ 28.34±1.29**△△

Figure 1

Pathological results in renal tissues of rats in different groups (HE staining, ×200)A: Normal group; B: Untreated group; C: Losartan group; D: Low-dose SQJDD group; E: Medium-dose SQJDD group; F: High-dose SQJDD group."

Table 3

Relative absorbance of TGF-β1, Smad2 and smad3 protein expressions in renal tissues of rats in different groups (x±s)"

Group n TGF-β1 Smad2 Smad3
Normal 15 0.269±0.073 0.326±0.075 0.323±0.092
Untreated 14 0.670±0.109** 0.821±0.118** 0.809±0.124**
Losartan 13 0.434±0.142**△△ 0.558±0.130**△△□ 0.487±0.142**△△□
Low-dose SQJDD 13 0.489±0.134**△△ 0.691±0.134**△△ 0.643±0.165**△△
Medium-dose SQJDD 15 0.472±0.130**△△ 0.618±0.103**△△ 0.589±0.131**△△
High-dose SQJDD 14 0.397±0.095**△△ 0.542±0.119**△△□ 0.471±0.131**△△□

Figure 2

Protein expression of TGF-β1 in renal tissues of rats in different groups 1: Losartan group; 2: High-dose SQJDD group; 3: Medium-dose SQJDD group; 4: Low-dose SQJDD group; 5: Untreated group; 6: Normal group."

Figure 3

Protein expression of Smad2 in renal tissues of rats in different groups1: Losartan group; 2: High-dose SQJDD group; 3: Medium-dose SQJDD group; 4: Low-dose SQJDD group; 5: Untreated group; 6: Normal group."

Figure 4

Protein expression of smad3 in renal tissues of rats in different groups1: Losartan group; 2: High-dose SQJDD group; 3: Medium-dose SQJDD group; 4: Low-dose SQJDD group; 5: Untreated group; 6: Normal group."

[1] Zhang Y, Si GM, Xu DM, Li Y . Clinical observation of “Shenqi Jiedu Decoction” in treating chronic renal failure of turbidity and blood-stasis syndrome and its effects on peripheral blood adhesion molecule[J]. Shanghai Zhong Yi Yao Za Zhi, 2008,42(3):26-28
张芸, 司国民, 许冬梅, 李云 . 参芪解毒汤治疗浊瘀内停型慢性肾衰竭的临床研究及对外周血黏附分子的影响[J]. 上海中医药杂志, 2008,42(3):26-28
[2] Chea SW, Lee KB . TGF-beta mediated epithelial-mesenchymal transition in autosomal dominant polycystic kidney disease[J]. Yonsei Med J, 2009,50(1):105-111
doi: 10.3349/ymj.2009.50.1.105
[3] Brown KA, Ham AJ, Clark CN, Meller N, Law BK, Chytil A, Cheng N, Pietenpol JA, Moses HL . Identification of novel Smad2 and Smad3 associated proteins in response to TGF-beta1[J]. J Cell Biochem, 2008,105(2):596-611
doi: 10.1002/jcb.v105:2
[4] Yokozawa T, Zheng PD, Oura H, Koizumi F . Animal model of adenine-induced chronic renal failure in rats[J]. Nephron, 1986,44(3):230-234
doi: 10.1159/000183992
[5] Geng J . Experimental study of rats with chronic renal failure induced by adenine[J]. Henan Zhong Yi Xue Yuan Xue Bao, 2008,23(6):24-25
耿静 . 腺嘌呤所致大鼠慢性肾功能衰竭的实验研究[J]. 河南中医学院学报, 2008,23(6):24-25
[6] Shi XY. Medical experimental zoology[M]. Beijing: People’s Military Medical Press, 2000: 332-335
施新猷 . 现代医学实验动物学[M]. 北京: 人民军医出版社, 2000: 332-335
[7] Li J, Campanale NV, Liang RJ, Deane JA, Bertram JF, Ricardo SD . Inhibition of p38 mitogen-activated protein kinase and transforming growth factor-beta1/Smad signaling pathways modulates the development of fibrosis in adriamycin-induced nephropathy[J]. Am J Pathol, 2006,169(5):1527-1540
doi: 10.2353/ajpath.2006.060169
[8] Wang W, Koka V, Lan HY . Transforming growth factor-beta and Smad signalling in kidney diseases[J]. Nephrology (Carlton), 2005,10(1):48-56
doi: 10.1111/nep.2005.10.issue-1
[9] Banas MC, Parks WT, Hudkins KL, Banas B, Holdren M, Iyoda M, Wietecha TA, Kowalewska J, Liu G, Alpers CE . Localization of TGF-beta signaling intermediates Smad2, 3, 4, and 7 in developing and mature human and mouse kidney[J]. J Histochem Cytochem, 2007,55(3):275-285
doi: 10.1369/jhc.6A7083.2006
[10] Trevisan R, Dodesini AR, Lepore G . Lipids and renal disease[J]. J Am Soc Nephrol, 2006,17(4 Suppl 2):S145-S147
doi: 10.1681/ASN.2005121320
[11] Tan YH, Hu P, Pei J, Li MF . Characteristics of lipid metabolism disturbance in children with primary nephritic syndrome and the clinical significance[J]. Guangxi Yi Ke Da Xue Xue Bao, 2006,23(3):389-391
覃远汉, 胡鹏, 裴娟, 李铭芳 . 小儿原发性肾病综合征脂质代谢紊乱特点及其临床意义[J]. 广西医科大学学报, 2006,23(3):389-391
[12] Deng W, Ding HG, Chen J . Treatment of chronic renal insufficiency with losartan and traditional Chinese drug for invigorating spleen and kidney[J]. J Chin Integr Med, 2006,4(4):425-426
邓玮, 丁昊谷, 陈菁 . 健脾补肾中药联合氯沙坦治疗慢性肾功能不全[J]. 中西医结合学报, 2006,4(4):425-426
[13] Zhao JJ, Lei MX, Zhou SX . Effect of endothelin-1 on the interaction of human mesangial cells and endothelial cells on the condition of lysophosphatidylcholine and the intervention effect of losartan[J]. Shandong Yi Yao, 2009,49(7):41-43
赵晋晋, 雷闽湘, 周素娴 . 高脂环境下ET-1在系膜细胞与内皮细胞相互关系中的作用及氯沙坦的干预作用[J]. 山东医药, 2009,49(7):41-43
[1] Shui-jie Shen, Yong-hong Zhang, Xiao-xia Gu, Shui-ju Jiang, Ling-jun Xu. Yangfei Kongliu Formula, a compound Chinese herbal medicine, combined with cisplatin, inhibits growth of lung cancer cells through transforming growth factor-β1 signaling pathway. Journal of Integrative Medicine, 2017, 15(3): 242-251.
[2] Lawrence A. Olatunji, Taofeek O. Usman, Joseph O. Adebayo, Victoria A. Olatunji . Effects of aqueous extract of Hibiscus sabdariffa on renal Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities in Wistar rats. Journal of Chinese Integrative Medicine, 2012, 10(9): 1049-1055.
[3] Na Wu , Lu Song , Xin-xin Yang, Jiang-lei Wei , Zhen-guo Liu. Effects of Chinese herbal medicine Tianqi Pingchan Granule on G protein-coupled receptor kinase 6 involved in the prevention of levodopa-induced dyskinesia in rats with Parkinson disease. Journal of Chinese Integrative Medicine, 2012, 10(9): 1018-1024.
[4] Dhirender Kaushik, Ajay Kumar, Pawan Kaushik, Avatar C. Rana. Anticonvulsant activity of alcoholic extract of bark of Pinus roxburghii Sarg. Journal of Chinese Integrative Medicine, 2012, 10(9): 1056-1060.
[5] Chan Chen, Chang-xun Chen, Xi-min Wu, Rui Wang, Yi-ming Li. Effects of extracts of Radix Scrophulariae on blood pressure in spontaneously hypertensive rats and the underlying mechanisms. Journal of Chinese Integrative Medicine, 2012, 10(9): 1009-1017.
[6] Jian-sheng Li, Ya Li, Su-yun Li, Yuan-yuan Wang, Li Deng, Yan-ge Tian, Su-li Jiang , Ying Wang. Long-term effects of Tiaobu Feishen therapies on systemic and local inflammation responses in rats with stable chronic obstructive pulmonary disease. Journal of Chinese Integrative Medicine, 2012, 10(9): 1039-1048.
[7] Karuna A. Talpate, Uma A. Bhosale, Mandar R. Zambare. Clitorea ternatea, a herb from Indian folklore, improves streptozotocin-induced diabetes and diabetes-induced cognitive decline in rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 939-947.
[8] Samuel Joshua Pragasam, Vachana Murunikara, Evan Prince Sabina, Mahaboobkhan Rasool. Antiperoxidative potential of p-coumaric acid, a common dietary phenol, in adjuvant-induced arthritis in rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 932-938.
[9] Zi-hui Li, Jian Wang, Rong-lin Cai, You-wen Wang, Jian-peng Hu. Establishment and evaluation of a rat model of ulcerative colitis with syndrome of dampness stagnancy due to spleen deficiency. Journal of Chinese Integrative Medicine, 2012, 10(8): 918-924.
[10] Hong Wang , Jing-tian Ou , Xin Zhao , Ying Guoz , Hao-ping Mao. Vasodilator effect of oroxylin A on thoracic aorta isolated from rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 880-885.
[11] Yong Zhang, Xiao-wen Sun, Jian-hua Xu, Hai Lu, Zhong-ze Fan, Jue Sun, Xiao-xiao Zhang. Effects of medicated serum prepared with Chinese herbal medicine Changweiqing on pharmacokinetics of oxaliplatin in colon cancer cells. Journal of Chinese Integrative Medicine, 2012, 10(8): 901-910.
[12] Xin-min Li, Hui Chen, Yan-yan Ren, Yan-li Lu. Effects of Chinese herbal medicine Xifeng Capsule on multidrug resistance-associated protein 1 expression in hippocampus and cortex of rats with lithium-pilocarpine-induced epilepsy. Journal of Chinese Integrative Medicine, 2012, 10(8): 911-917.
[13] Meng Li, Ling Hu, Rong-lin Cai, Zi-jian Wu, Ke-ming Wang. Effects of electro-acupuncture at PC6 and BL15 on nerve electrical activity in spinal dorsal root and norepinephrine and dopamine contents in paraventricular nucleus of hypothalamus in rats with acute myocardial ischemia. Journal of Chinese Integrative Medicine, 2012, 10(8): 874-879.
[14] C. Mouli Kalla, Vijaya Tartte, Dattatreya Rao Sirpurkar. Effectiveness of flavonoid-rich leaf extract of Acalypha indica in reversing experimental myocardial ischemia: biochemical and histopathological evidence. Journal of Chinese Integrative Medicine, 2012, 10(7): 784-792.
[15] Feng Xin-ying, Zhu Min, Zhang Qi-qi, Chen Yi-ping, Li Wen-wei. Selective protection of nigral dopaminergic neurons by echinacoside in a rat model of Parkinson disease induced by rotenone. Journal of Chinese Integrative Medicine, 2012, 10(7): 777-783.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. An explanation on "guiding principles of clinical research on mild cognitive impairment (protocol)". Journal of Chinese Integrative Medicine, 2008, 6(1): 15-21
[2] Yi-ting He, Qing-lin Zha, Jian-ping Yu, Yong Tan, Cheng Lu, Ai-ping Lv. Principal factor analysis of symptoms of rheumatoid arthritis and their correlations with efficacy of traditional Chinese medicine and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 32-36
[3] Jun Cai, Hua Wang, Sheng Zhou, Bin Wu, Hua-rong Song, Zheng-rong Xuan. Effect of Sijunzi Decoction and enteral nutrition on T-cell subsets and nutritional status in patients with gastric cancer after operation: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(1): 37-40
[4] Wei Zhang, Xiang-feng Lu, Xiao-mei Zhang, Jian-jun Wu, Liang-duo Jiang. A rat model of pulmonary fibrosis induced by infusing bleomycin quickly through tracheal intubation. Journal of Chinese Integrative Medicine, 2008, 6(1): 60-67
[5] A-gao Zhou, Yong Zhang, Gang Kui, De-Yun Kong, Hai-liang Ge, Qiu-hua Ren, Jia-rong Dong, Sheng Hong, Xu-ming Mao, Yin Wang, Hui-zheng Zhang, Shu-jun Wang. Influence of traditional Chinese compound recipes with different efficacy on body weight, tumor weight and immune function in H22 cancer-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 77-82
[6] Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[7] Li Zhou, Hong-xing Zhang, Ling-guang Liu, Wen-jun Wan. Effect of electro-acupuncture at Fenglong (GV 16) on nitric oxide and endothelin in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 89-92
[8] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. Guiding principles of clinical research on mild cognitive impairment (protocol). Journal of Chinese Integrative Medicine, 2008, 6(1): 9-14
[9] Xin-jun Wang, Ling-ling Wang . A mechanism of endogenous opioid peptides for rapid onset of acupuncture effect in treatment of depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1014-1017
[10] Bo Wang , Wei Yan , Li-hui Hou, Xiao-ke Wu. Disorder of Tiangui (kidney essence) and reproductive dysfunction in patients with polycystic ovary syndrome. Journal of Chinese Integrative Medicine, 2010, 8(11): 1018-1022
[an error occurred while processing this directive]