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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (3): 263-268.doi: 10.3736/jcim20100311

• Original Experimental Research • Previous Articles     Next Articles

Effects of Shenqi Jiedu Decoction on expressions of transforming growth factor-β1, smad2 and smad3 in renal tissues of rats with chronic renal failure induced by adenine

 Ting-ting Lia, Guo-min Sia, Feng-cui Chenb   

  1. a Department of Traditional Chinese Medicine, Shandong Provincial Hospital, Shandong University, Jinan 250021, Shandong Province, China
    b Department of Pediatrics, Rushan Hospital of Traditional Chinese Medicine, Rushan 264500, Shandong Province, China
  • Received:2009-11-09 Accepted:2010-01-10 Online:2010-03-20 Published:2010-03-15
  • Contact: Guo-min Si E-mail:sgm977@126.com

Objective

To investigate the effects of Shenqi Jiedu Decoction (SQJDD), a compound traditional Chinese herbal medicine, on expressions of transforming growth factor beta1 (TGF-β1), smad2 and smad3 proteins in renal tissues of rats with chronic renal failure (CRF) induced by adenine, and to explore the mechanisms of SQJDD in treating CRF.
Methods

Ninety healthy Wistar male rats were randomly divided into six groups: normal group, untreated group, losartan group, low-dose SQJDD group, medium-dose SQJDD group, and high-dose SQJDD group. Rat model of CRF was established by feeding 2.5% adenine. Levels of plasma creatinine (Cr) and blood urea nitrogen (BUN) in different groups after model establishment were detected to estimate the renal function of rats with CRF. After eight-week treatment, levels of Cr, BUN, triacylglycerol (TAG), cholesterol (Chol) and albumin (ALB) in plasma were detected. Pathological changes of renal tissues were observed by HE staining. Protein expressions of TGF-β1, Smad2 and Smad3 in renal tissues were detected by Western blotting.
Results

Compared with pretreatment, levels of Cr and BUN in the losartan group and the SQJDD groups were markedly decreased (P<0.01). After treatment, levels of Cr, BUN and Chol in all treated groups were lower than those in the untreated group (P<0.01, P<0.05). Levels of ALB in all treated groups were higher than that in the untreated group (P<0.01). Pathological lesions of renal tissues in all treated groups were lessened as compared with the untreated group (P<0.01). The Western blotting results showed that the protein expressions of TGF-β1, Smad2 and Smad3 in renal tissues in all treated groups were lower than those in the untreated group (P<0.01).
Conclusion

SQJDD can delay the progress of CRF by inhibiting TGF-β1/Smads signal transduction pathway.

Key words: Chronic renal failure, Compound (TCD), Transforming growth factor betal, Smad2, Smad3, Rats

Table 1

Plasma Cr and BUN levels of rats in different groups after model establishment and treatment (x±s)"

Group n Cr (μmol/L) BUN (mmol/L)
Before treatment After treatment Before treatment After treatment
Normal 15 50.62±10.04 50.45±8.55 8.05±1.10 8.20±0.85
Untreated 14 123.62±18.37** 122.10±17.40** 47.89±4.06** 45.41±3.62**
Losartan 13 118.58±15.14** 90.10±2.90**△△▲▲ 48.32±3.89** 30.82±1.55**△△▲▲
Low-dose SQJDD 13 122.40±18.48** 97.72±5.07**△△▲▲ 45.79±3.59** 30.90±3.91**△△▲▲
Medium-dose SQJDD 15 119.78±14.23** 93.09±5.55**△△▲▲ 48.53±3.92** 32.76±2.08**△△▲▲
High-dose SQJDD 14 120.43±14.50** 90.53±3.00**△△▲▲ 46.06±2.33** 29.81±4.04**△△▲▲

Table 2

Plasma TAG, Chol and ALB levels of rats in different groups after treatment (x±s)"

Group n TAG (mmol/L) Chol (mmol/L) ALB (g/L)
Normal 15 0.73±0.10 1.04±0.11 30.53±1.30
Untreated 14 1.25±0.10** 1.90±0.19** 22.80±1.82**
Losartan 13 1.07±0.16** 1.68±0.18**△ 27.89±1.52**△△
Low-dose SQJDD 13 1.14±0.26** 1.75±0.23**△ 26.40±1.65**△△
Medium-dose SQJDD 15 1.11±0.25** 1.70±0.21**△ 27.09±1.16**△△
High-dose SQJDD 14 1.05±0.13** 1.68±0.16**△ 28.34±1.29**△△

Figure 1

Pathological results in renal tissues of rats in different groups (HE staining, ×200)A: Normal group; B: Untreated group; C: Losartan group; D: Low-dose SQJDD group; E: Medium-dose SQJDD group; F: High-dose SQJDD group."

Table 3

Relative absorbance of TGF-β1, Smad2 and smad3 protein expressions in renal tissues of rats in different groups (x±s)"

Group n TGF-β1 Smad2 Smad3
Normal 15 0.269±0.073 0.326±0.075 0.323±0.092
Untreated 14 0.670±0.109** 0.821±0.118** 0.809±0.124**
Losartan 13 0.434±0.142**△△ 0.558±0.130**△△□ 0.487±0.142**△△□
Low-dose SQJDD 13 0.489±0.134**△△ 0.691±0.134**△△ 0.643±0.165**△△
Medium-dose SQJDD 15 0.472±0.130**△△ 0.618±0.103**△△ 0.589±0.131**△△
High-dose SQJDD 14 0.397±0.095**△△ 0.542±0.119**△△□ 0.471±0.131**△△□

Figure 2

Protein expression of TGF-β1 in renal tissues of rats in different groups 1: Losartan group; 2: High-dose SQJDD group; 3: Medium-dose SQJDD group; 4: Low-dose SQJDD group; 5: Untreated group; 6: Normal group."

Figure 3

Protein expression of Smad2 in renal tissues of rats in different groups1: Losartan group; 2: High-dose SQJDD group; 3: Medium-dose SQJDD group; 4: Low-dose SQJDD group; 5: Untreated group; 6: Normal group."

Figure 4

Protein expression of smad3 in renal tissues of rats in different groups1: Losartan group; 2: High-dose SQJDD group; 3: Medium-dose SQJDD group; 4: Low-dose SQJDD group; 5: Untreated group; 6: Normal group."

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