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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (3): 263-268.doi: 10.3736/jcim20100311

• Original Experimental Research • Previous Articles     Next Articles

Effects of Shenqi Jiedu Decoction on expressions of transforming growth factor-β1, smad2 and smad3 in renal tissues of rats with chronic renal failure induced by adenine

 Ting-ting Lia, Guo-min Sia, Feng-cui Chenb   

  1. a Department of Traditional Chinese Medicine, Shandong Provincial Hospital, Shandong University, Jinan 250021, Shandong Province, China
    b Department of Pediatrics, Rushan Hospital of Traditional Chinese Medicine, Rushan 264500, Shandong Province, China
  • Received:2009-11-09 Accepted:2010-01-10 Online:2010-03-20 Published:2010-03-15
  • Contact: Guo-min Si E-mail:sgm977@126.com

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Objective

To investigate the effects of Shenqi Jiedu Decoction (SQJDD), a compound traditional Chinese herbal medicine, on expressions of transforming growth factor beta1 (TGF-β1), smad2 and smad3 proteins in renal tissues of rats with chronic renal failure (CRF) induced by adenine, and to explore the mechanisms of SQJDD in treating CRF.
Methods

Ninety healthy Wistar male rats were randomly divided into six groups: normal group, untreated group, losartan group, low-dose SQJDD group, medium-dose SQJDD group, and high-dose SQJDD group. Rat model of CRF was established by feeding 2.5% adenine. Levels of plasma creatinine (Cr) and blood urea nitrogen (BUN) in different groups after model establishment were detected to estimate the renal function of rats with CRF. After eight-week treatment, levels of Cr, BUN, triacylglycerol (TAG), cholesterol (Chol) and albumin (ALB) in plasma were detected. Pathological changes of renal tissues were observed by HE staining. Protein expressions of TGF-β1, Smad2 and Smad3 in renal tissues were detected by Western blotting.
Results

Compared with pretreatment, levels of Cr and BUN in the losartan group and the SQJDD groups were markedly decreased (P<0.01). After treatment, levels of Cr, BUN and Chol in all treated groups were lower than those in the untreated group (P<0.01, P<0.05). Levels of ALB in all treated groups were higher than that in the untreated group (P<0.01). Pathological lesions of renal tissues in all treated groups were lessened as compared with the untreated group (P<0.01). The Western blotting results showed that the protein expressions of TGF-β1, Smad2 and Smad3 in renal tissues in all treated groups were lower than those in the untreated group (P<0.01).
Conclusion

SQJDD can delay the progress of CRF by inhibiting TGF-β1/Smads signal transduction pathway.

Key words: Chronic renal failure, Compound (TCD), Transforming growth factor betal, Smad2, Smad3, Rats

Table 1

Plasma Cr and BUN levels of rats in different groups after model establishment and treatment (x±s)"

Group n Cr (μmol/L) BUN (mmol/L)
Before treatment After treatment Before treatment After treatment
Normal 15 50.62±10.04 50.45±8.55 8.05±1.10 8.20±0.85
Untreated 14 123.62±18.37** 122.10±17.40** 47.89±4.06** 45.41±3.62**
Losartan 13 118.58±15.14** 90.10±2.90**△△▲▲ 48.32±3.89** 30.82±1.55**△△▲▲
Low-dose SQJDD 13 122.40±18.48** 97.72±5.07**△△▲▲ 45.79±3.59** 30.90±3.91**△△▲▲
Medium-dose SQJDD 15 119.78±14.23** 93.09±5.55**△△▲▲ 48.53±3.92** 32.76±2.08**△△▲▲
High-dose SQJDD 14 120.43±14.50** 90.53±3.00**△△▲▲ 46.06±2.33** 29.81±4.04**△△▲▲

Table 2

Plasma TAG, Chol and ALB levels of rats in different groups after treatment (x±s)"

Group n TAG (mmol/L) Chol (mmol/L) ALB (g/L)
Normal 15 0.73±0.10 1.04±0.11 30.53±1.30
Untreated 14 1.25±0.10** 1.90±0.19** 22.80±1.82**
Losartan 13 1.07±0.16** 1.68±0.18**△ 27.89±1.52**△△
Low-dose SQJDD 13 1.14±0.26** 1.75±0.23**△ 26.40±1.65**△△
Medium-dose SQJDD 15 1.11±0.25** 1.70±0.21**△ 27.09±1.16**△△
High-dose SQJDD 14 1.05±0.13** 1.68±0.16**△ 28.34±1.29**△△

Figure 1

Pathological results in renal tissues of rats in different groups (HE staining, ×200)A: Normal group; B: Untreated group; C: Losartan group; D: Low-dose SQJDD group; E: Medium-dose SQJDD group; F: High-dose SQJDD group."

Table 3

Relative absorbance of TGF-β1, Smad2 and smad3 protein expressions in renal tissues of rats in different groups (x±s)"

Group n TGF-β1 Smad2 Smad3
Normal 15 0.269±0.073 0.326±0.075 0.323±0.092
Untreated 14 0.670±0.109** 0.821±0.118** 0.809±0.124**
Losartan 13 0.434±0.142**△△ 0.558±0.130**△△□ 0.487±0.142**△△□
Low-dose SQJDD 13 0.489±0.134**△△ 0.691±0.134**△△ 0.643±0.165**△△
Medium-dose SQJDD 15 0.472±0.130**△△ 0.618±0.103**△△ 0.589±0.131**△△
High-dose SQJDD 14 0.397±0.095**△△ 0.542±0.119**△△□ 0.471±0.131**△△□

Figure 2

Protein expression of TGF-β1 in renal tissues of rats in different groups 1: Losartan group; 2: High-dose SQJDD group; 3: Medium-dose SQJDD group; 4: Low-dose SQJDD group; 5: Untreated group; 6: Normal group."

Figure 3

Protein expression of Smad2 in renal tissues of rats in different groups1: Losartan group; 2: High-dose SQJDD group; 3: Medium-dose SQJDD group; 4: Low-dose SQJDD group; 5: Untreated group; 6: Normal group."

Figure 4

Protein expression of smad3 in renal tissues of rats in different groups1: Losartan group; 2: High-dose SQJDD group; 3: Medium-dose SQJDD group; 4: Low-dose SQJDD group; 5: Untreated group; 6: Normal group."

[1] Zhang Y, Si GM, Xu DM, Li Y . Clinical observation of “Shenqi Jiedu Decoction” in treating chronic renal failure of turbidity and blood-stasis syndrome and its effects on peripheral blood adhesion molecule[J]. Shanghai Zhong Yi Yao Za Zhi, 2008,42(3):26-28
张芸, 司国民, 许冬梅, 李云 . 参芪解毒汤治疗浊瘀内停型慢性肾衰竭的临床研究及对外周血黏附分子的影响[J]. 上海中医药杂志, 2008,42(3):26-28
[2] Chea SW, Lee KB . TGF-beta mediated epithelial-mesenchymal transition in autosomal dominant polycystic kidney disease[J]. Yonsei Med J, 2009,50(1):105-111
doi: 10.3349/ymj.2009.50.1.105
[3] Brown KA, Ham AJ, Clark CN, Meller N, Law BK, Chytil A, Cheng N, Pietenpol JA, Moses HL . Identification of novel Smad2 and Smad3 associated proteins in response to TGF-beta1[J]. J Cell Biochem, 2008,105(2):596-611
doi: 10.1002/jcb.v105:2
[4] Yokozawa T, Zheng PD, Oura H, Koizumi F . Animal model of adenine-induced chronic renal failure in rats[J]. Nephron, 1986,44(3):230-234
doi: 10.1159/000183992
[5] Geng J . Experimental study of rats with chronic renal failure induced by adenine[J]. Henan Zhong Yi Xue Yuan Xue Bao, 2008,23(6):24-25
耿静 . 腺嘌呤所致大鼠慢性肾功能衰竭的实验研究[J]. 河南中医学院学报, 2008,23(6):24-25
[6] Shi XY. Medical experimental zoology[M]. Beijing: People’s Military Medical Press, 2000: 332-335
施新猷 . 现代医学实验动物学[M]. 北京: 人民军医出版社, 2000: 332-335
[7] Li J, Campanale NV, Liang RJ, Deane JA, Bertram JF, Ricardo SD . Inhibition of p38 mitogen-activated protein kinase and transforming growth factor-beta1/Smad signaling pathways modulates the development of fibrosis in adriamycin-induced nephropathy[J]. Am J Pathol, 2006,169(5):1527-1540
doi: 10.2353/ajpath.2006.060169
[8] Wang W, Koka V, Lan HY . Transforming growth factor-beta and Smad signalling in kidney diseases[J]. Nephrology (Carlton), 2005,10(1):48-56
doi: 10.1111/nep.2005.10.issue-1
[9] Banas MC, Parks WT, Hudkins KL, Banas B, Holdren M, Iyoda M, Wietecha TA, Kowalewska J, Liu G, Alpers CE . Localization of TGF-beta signaling intermediates Smad2, 3, 4, and 7 in developing and mature human and mouse kidney[J]. J Histochem Cytochem, 2007,55(3):275-285
doi: 10.1369/jhc.6A7083.2006
[10] Trevisan R, Dodesini AR, Lepore G . Lipids and renal disease[J]. J Am Soc Nephrol, 2006,17(4 Suppl 2):S145-S147
doi: 10.1681/ASN.2005121320
[11] Tan YH, Hu P, Pei J, Li MF . Characteristics of lipid metabolism disturbance in children with primary nephritic syndrome and the clinical significance[J]. Guangxi Yi Ke Da Xue Xue Bao, 2006,23(3):389-391
覃远汉, 胡鹏, 裴娟, 李铭芳 . 小儿原发性肾病综合征脂质代谢紊乱特点及其临床意义[J]. 广西医科大学学报, 2006,23(3):389-391
[12] Deng W, Ding HG, Chen J . Treatment of chronic renal insufficiency with losartan and traditional Chinese drug for invigorating spleen and kidney[J]. J Chin Integr Med, 2006,4(4):425-426
邓玮, 丁昊谷, 陈菁 . 健脾补肾中药联合氯沙坦治疗慢性肾功能不全[J]. 中西医结合学报, 2006,4(4):425-426
[13] Zhao JJ, Lei MX, Zhou SX . Effect of endothelin-1 on the interaction of human mesangial cells and endothelial cells on the condition of lysophosphatidylcholine and the intervention effect of losartan[J]. Shandong Yi Yao, 2009,49(7):41-43
赵晋晋, 雷闽湘, 周素娴 . 高脂环境下ET-1在系膜细胞与内皮细胞相互关系中的作用及氯沙坦的干预作用[J]. 山东医药, 2009,49(7):41-43
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