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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (11): 1048-1052.doi: 10.3736/jcim20101108

• Original Expreimental Research • Previous Articles     Next Articles

Senescence-inducing effects of Chinese herbal medicine Tenglong Buzhong Decoction on human colon carcinoma LS-174-T cells and the mechanism

Bing Hu a,b,Hong-mei Anc,Ke-ping Shena, Qin Dua   

  1. a 5th Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    b E-institute of Internal Medicine of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    c Department of Science and Technology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2010-02-23 Accepted:2010-07-13 Online:2010-11-20 Published:2010-11-15
  • Contact: Bing Hu E-mail:beearhu@hotmail.com

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Objective

Cell senescence is an important anti-cancer mechanism and may contribute to cancer therapeutic outcome. The present study observed the effects of Tenglong Buzhong Decoction (TLBZD), a Chinese herbal formula, on senescence in human colon carcinoma LS-174-T cells.

Methods

LS-174-T cells were treated with TLBZD, and morphology change was observed under a microscope. Cell senescence was identified by senescence-associated β-galactosidase (SA-β-gal) staining, and cell cycle was analyzed by flow cytometry. Expressions of p53, p21 WAF1/CIP1, p16 and RB and RB phosphorylation were detected by Western blot.

Results

After being treated with TLBZD, LS-174-T cells became enlarged and flattened by morphology; SA-β-gal staining was positive and cell cycle was arrested in G0/G1. In addition, up-regulations of p21 WAF1/CIP1 and p16, as well as inhibition of RB phosphorylation were detected in response to TLBZD treatment. Expressions of p53 and RB were unchanged after TLBZD treatment.

Conclusion

TLBZD is effective in inducing cell senescence in human colon carcinoma LS-174-T cells, which may relate to up-regulations of p21 WAF1/CIP1 and p16 and inhibition of RB phosphorylation.

Key words: Colonic neoplasms, Chinese herbal drugs, Cell senescence, Signal transduction

Figure 1

Effects of TLBZD on activities of SA-β-gal in LS-174-T cells and CRL-1790 cells (Light microscopy, ×200) A and B: LS-174-T cells; C and D: CRL-1790 cells; A and C: ddH2O; B and D: TLBZD."

Figure 2

Effects of TLBZD on cell cycle in LS-174-T cells"

Table 1

Effects of TLBZD on cell cycle in LS-174-T cells"

Figure 3

Effects of TLBZD on expressions of senescence-related genes in LS-174-T cells"

[1] Jemal A, Siegel R, Ward E, Hao Y, Xu J, Thun MJ . Cancer statistics, 2009[J]. CA Cancer J Clin, 2009,59(4):225-249
doi: 10.3322/caac.20006
[2] Yance DR Jr, Sagar SM . Targeting angiogenesis with integrative cancer therapies[J]. Integr Cancer Ther, 2006,5(1):9-29
doi: 10.1177/1534735405285562
[3] Sagar SM, Yance D, Wong RK . Natural health products that inhibit angiogenesis: a potential source for investigational new agents to treat cancer — Part 1[J]. Curr Oncol, 2006,13(1):14-26
[4] Chiantore MV, Vannucchi S, Mangino G, Percario ZA, Affabris E, Fiorucci G, Romeo G . Senescence and cell death pathways and their role in cancer therapeutic outcome[J]. Curr Med Chem, 2009,16(3):287-300
doi: 10.2174/092986709787002691
[5] Gewirtz DA, Holt SE, Elmore LW . Accelerated senescence: an emerging role in tumor cell response to chemotherapy and radiation[J]. Biochem Pharmacol, 2008,76(8):947-957
doi: 10.1016/j.bcp.2008.06.024 pmid: 18657518
[6] Hsu YL, Kuo PL, Tzeng TF, Sung SC, Yen MH, Lin LT, Lin CC . Huang-lian-jie-du-tang, a traditional Chinese medicine prescription, induces cell-cycle arrest and apoptosis in human liver cancer cells in vitro and in vivo[J]. J Gastroenterol Hepatol, 2008,23(7 Pt 2):e290-e299
doi: 10.1111/jgh.2008.23.issue-7pt2
[7] Kao ST, Yeh CC, Hsieh CC, Yang MD, Lee MR, Liu HS, Lin JG . The Chinese medicine Bu-Zhong-Yi-Qi-Tang inhibited proliferation of hepatoma cell lines by inducing apoptosis via G0/G1 arrest[J]. Life Sci, 2001,69(13):1485-1496
doi: 10.1016/S0024-3205(01)01226-7
[8] Du P, Lu JF . Effect of Zuogui Pill extracts of water extracting-alcohol precipitation on rat ovarian granulosa cell growth in vitro[J]. Zhong Yao Yao Li Yu Lin Chuang, 2007,23(1):9-10
杜萍, 卢金福 . 左归丸水提醇沉液对体外培养大鼠卵巢颗粒细胞生长的影响[J]. 中药药理与临床, 2007,23(1):9-10
[9] Dimri GP, Lee X, Basile G, Acosta M, Scott G, Roskelley C, Medrano EE, Linskens M, Rubelj I, Pereira-Smith O, Peacocke M, Campisi S . A biomarker that identifies senescent human cells in culture and in aging skin in vivo[J]. Proc Natl Acad Sci U S A, 1995,92(20):9363-9367
doi: 10.1073/pnas.92.20.9363 pmid: 7568133
[10] Hu B, An HM, Shen KP . Cellular senescence and carcinogenesis[J]. Sheng Ming Ke Xue, 2008,20(3):447-449
doi: 10.3760/cma.j.issn.1673-422X.2010.10.001
胡兵, 安红梅, 沈克平 . 细胞衰老与肿瘤发生[J]. 生命科学, 2008,20(3):447-449
doi: 10.3760/cma.j.issn.1673-422X.2010.10.001
[11] Chen Z, Trotman LC, Shaffer D, Lin HK, Dotan ZA, Niki M, Koutcher JA, Scher HI, Ludwig T, Gerald W, Cordon-Cardo C, Pandolfi PP . Crucial role of p53-dependent cellular senescence in suppression of Pten-deficient tumorigenesis[J]. Nature, 2005,436(7051):725-730
doi: 10.1038/nature03918 pmid: 16079851
[12] Michaloglou C, Vredeveld LC, Soengas MS, Denoyelle C, Kuilman T, van der Horst CM, Majoor DM, Shay JW, Mooi WJ, Peeper DS. BRAFE600-associated senescence-like cell cycle arrest of human naevi[J]. Nature, 2005,436(7051):720-724
doi: 10.1038/nature03890 pmid: 16079850
[13] Collado M, Gil J, Efeyan A, Guerra C, Schuhmacher AJ, Barradas M, Bengur a A,Zaballos A,Flores JM,Barbacid M,Beach D,Serrano M. Tumour biology: senescence in premalignant tumours[J]. Nature, 2005,436(7051):642
doi: 10.1038/436642a
[14] Braig M, Lee S, Loddenkemper C, Rudolph C, Peters AH, Schlegelberger B, Stein H , Dörken B, Jenuwein T, Schmitt CA. Oncogene-induced senescence as an initial barrier in lymphoma development[J]. Nature, 2005,436(7051):660-665
doi: 10.1038/nature03841 pmid: 16079837
[15] Han Z, Wei W, Dunaway S, Darnowski JW, Calabresi P, Sedivy J, Hendrickson EA, Balan KV, Pantazis P, Wyche JH . Role of p21 in apoptosis and senescence of human colon cancer cells treated with camptothecin[J]. J Biol Chem, 2002,277(19):17154-17160
doi: 10.1074/jbc.M112401200
[16] Thaler S, Hähnel PS, Schad A, Dammann R, Schuler M . RASSF1A mediates p21Cip1/Waf1-dependent cell cycle arrest and senescence through modulation of the Raf-MEK-ERK pathway and inhibition of Akt[J]. Cancer Res, 2009,69(5):1748-1757
doi: 10.1158/0008-5472.CAN-08-1377
[17] Aliouat-Denis CM, Dendouga N, Van den Wyngaert I, Goehlmann H, Steller U, van de Weyer I, Van Slycken N, Andries L,Kass S,Luyten W,Janicot M,Vialard JE. p53-independent regulation of p21WAF1/CIP1 expression and senescence by Chk2[J]. Mol Cancer Res, 2005,3(11):627-634
doi: 10.1158/1541-7786.MCR-05-0121 pmid: 16317088
[18] Kang JY, Kim JJ, Jang SY, Bae YS . The p53-p21Cip1/Waf1 pathway is necessary for cellular senescence induced by the inhibition of protein kinase CKII in human colon cancer cells[J]. Mol Cells, 2009,28(5):489-494
doi: 10.1007/s10059-009-0141-9
[19] Papazoglu C , Mills AA.p53: at the crossroad between cancer and ageing[J]. J Pathol, 2007,211(2):124-133
doi: 10.1002/path.2086 pmid: 17200941
[20] Varna M, Lehmann-Che J, Turpin E, Marangoni E, El-Bouchtaoui M, Jeanne M, Grigoriu C, Ratajczak P, Leboeuf C, Plassa LF, Ferreira I, Poupon MF , Janin A, de Thé H, Bertheau P.p53 dependent cell-cycle arrest triggered by chemotherapy in xenografted breast tumors[J]. Int J Cancer, 2009,124(4):991-997
doi: 10.1002/ijc.24049 pmid: 19048622
[21] Dimri GP . What has senescence got to do with cancer[J]. Cancer Cell, 2005,7(6):505-512
doi: 10.1016/j.ccr.2005.05.025 pmid: 1769521
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