"

Figure 1

Inhibitory effect of FYN on increase in iNOS mRNA induced by TNF-α in A549 cells The cells were incubated for 24 h. iNOS mRNA was determined by real-time polymerase chain reaction. Data are expressed as mean±standard deviation; n=3; **P<0.01, vs control group; △△P<0.01, vs TNF-α group. a: Control group; b: TNF-α group; c: FYN alone group (15% serum containing FYN); d: FNY plus TNF-α group. FYN: Feiyanning Decoction; iNOS: inducible nitric oxide synthase; TNF-α: tumor necrosis factor-α; A549: human lung adenocarcinoma epithelial cell line."

Figure 2

Inhibitory effect of FYN on increase in iNOS expression induced by TNF-α in A549 cells The cells were incubated for 24 h. iNOS expression was determined by Western blot. Data are expressed as mean±standard deviation; n=3; **P<0.01, vs control group; △△P<0.01, vs TNF-α group. a: Control group; b: TNF-α (1 μg/L) group; c: FYN alone group (15% serum containing FYN); d: FYN plus TNF-α group. FYN: Feiyanning Decoction; iNOS: inducible nitric oxide synthase; TNF-α: tumor necrosis factor-α; A549: human lung adenocarcinoma epithelial cell line."

Figure 3

Inhibitory effect of FYN on increase in NO production induced by TNF-α in A549 cells The cells were incubated for 24 h. The DAF-2 assay was performed. Data are expressed as mean±standard deviation; n=3; **P<0.01 vs control group; △△P<0.01, vs TNF-α group. a: Control group; b: TNF-α group; c: FYN alone group (15% serum containing FYN); d: FYN plus TNF-α group. FYN: Feiyanning Decoction; NO: nitric oxide; TNF-α: tumor necrosis factor-α; A549: human lung adenocarcinoma epithelial cell line."

Figure 4

Effect of FYN on iNOS promoter in A549 cells Cells plated in six-well plates were transfected with iNOS-luciferase plasmid. The cells were treated for 24 h after transfection, and then luciferase activity in the cell lysates was measured. Data are expressed as mean±standard deviation; n=3; **P<0.01, vs control group; △△P<0.01, vs TNF-α group. a: Control group; b: TNF-α group; c: FYN alone group (15% serum containing FYN); d: FYN plus TNF-α group. FYN: Feiyanning Decoction; iNOS: inducible nitric oxide synthase; TNF-α: tumor necrosis factor-α; A549: human lung adenocarcinoma epithelial cell line."

Figure 5

Inhibitory effect of FYN on increase in COX-2 mRNA induced by TNF-α in A549 cells The cells were incubated for 24 h. COX-2 mRNA was determined by real-time polymerase chain reaction. Data are expressed as mean±standard deviation; n=3; **P<0.01, vs control group; △△P<0.01, vs TNF-α group. a: Control group; b: TNF-α group; c: FYN alone group (15% serum containing FYN); d: FYN plus TNF-α group. FYN: Feiyanning Decoction; COX-2: cyclooxygenase-2; TNF-α: tumor necrosis factor-α; A549: human lung adenocarcinoma epithelial cell line."

Figure 6

Inhibitory effect of FYN on COX-2 expression induced by TNF-α in A549 cells The cells were incubated for 24 h. COX-2 expression was determined by Western blotting. Data are expressed as mean±standard deviation; n=3; **P<0.01, vs control group; △△P<0.01, vs TNF-α group. a: Control group; b: TNF-α group; c: FYN alone group (15% serum containing FYN); d: FYN plus TNF-α group; FYN: Feiyanning Decoction; COX-2: cyclooxygenase-2; TNF-α: tumor necrosis factor-α; A549: human lung adenocarcinoma epithelial cell line."