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Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (1): 30-32.doi: 10.3736/jcim20040111

• Original Experimental Research • Previous Articles     Next Articles

Effect of curcumin on extracellular matrix accumulation in the glomeruli in nephrotoxic sera nephritis rats

Hua-ying Bao1, Rong-hua Chen2, Song-ming Huang1, Ai-hua Zhang1, Mei Guo2, Li Fei2, Xiao-qing Pan2   

  1. 1. Department of Nephrology,Nanjing Children’s Hospital, Nanjing Medical University, Nanjing, Jiangsu Province 210008, China;
    2. Institute of Children Nephrology, Nanjing Medical University, Nanjing, Jiangsu Province 210008, China
  • Online:2004-01-20 Published:2018-10-25

Objective: To observe whether curcumin could inhibit the accumulation of the collagen Ⅳ and fibronectin in the glomeruli in nephrotoxi sera nephritis rats. Methods: Seventy-two healthy male Sprague-Dawley rats were divided into three groups, with 24 animals in each group. For normal control group, normal saline (0.5 ml/d) was injected through intra-caudal-vein for two days, and at the same time normal saline (0.5 ml/kg) was also daily administered intraperitoneally. For nephrotoxic sera nephritis group, nephrotoxic sera (0.5 ml/d) was injected through the tail vein for two days and dimethyl sulfoxide (0.5 ml/kg) was given intraperitoneally daily. For curcumin group, nephrotoxic sera was injected as above and meanwhile curcumin (50 mg·kg-1·d-1) was administered intraperitoneally every day. Six rats in each group were killed on the 3rd, 7th, 14th and 28th day. Their renal tissue was fixed in 10% formalin for examining the expression of collagen Ⅳ and fibronectin. Results: Minimal staining of collagen Ⅳ and fibronectin was detected in the basement membrane of normal control rats glomeruli. In the nephrotoxic sera nephritis rats and curcumin treated nephrotoxic sera nephritis rats, the accumulation of collagen Ⅳ and fibronectin was increased progressively, with significant difference in the accumulation of collagen Ⅳ(P <0.01) between these two groups at the same time points, while the significant difference in fibronectin accumulation (P <0.05) appeared only after the 7th days.Conclusion: Curcumin can reduce the accumulation of collagen Ⅳ and fibronectin in the glomeruli. Hence we postulated that curcumin might have beneficial effect for retarding glomerulosclerosis.

Key words: curcumin, nephrotoxic sera nephritis, collagen Ⅳ, fibronectin

CLC Number: 

  • R692.31

Tab 1

Positive expression area of collagen Ⅳ in glomeruli of rats at different time (ヌ±S, %)"

Groupn3rd day7th day14th day28th day
Normal control group62.40±0.652.15±0.332.08±0.322.58±0.41
Nephrotoxic sera nephritis group610.20±2.6116.00±1.84△△27.64±5.21△△27.24±4.03△△
Curcumin group64.08±2.25**7.78±2.45△△**9.64±1.81△△**13.35±4.60△△**

Tab 2

Positive expression area of fibronectin in glomeruli of rats at different time (ヌ±S, %)"

Groupn3rd day7th day14th day28th day
Normal control group62.82±1.335.43±2.617.04±1.657.46±1.55
Nephrotoxic sera nephritis group615.44±1.86△△19.30±3.69△△23.53±3.74△△29.41±2.63△△
Curcumin group612.44±5.04△△15.40±2.88△△*17.22±3.46△△*18.72±3.25△△**
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