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Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (5): 350-352.doi: 10.3736/jcim20040512

• Original Experimental Research • Previous Articles     Next Articles

Effects of polyporus polysaccharide on activity and mRNA expression of inducible nitric oxide synthase in peritoneal macrophages of mice

Di-nan Huang(), Gan Hou, Qi-feng Zhu   

  1. Department of Biochemistry, Guangdong Medical College, Zhanjiang, Guangdong Province 524023, China
  • Received:2003-11-19 Online:2004-09-20 Published:2018-10-20

Objective: To study the mechanisms of the antitumor and immunoregulation functions of polyporus polysaccharide (PPS).Methods: The production of nitric oxide (NO), the activity and mRNA expression of inducible nitric oxide synthase (iNOS) in peritoneal macrophages of mice administered with different dose of PPS were observed by Griess reaction, fluorimetry assay and RT-PCR, respectively.Results: PPS could elevate the iNOS activity with dose-dependence and stimulate the iNOS mRNA expression of peritoneal macrophages in mice.Conclusion: The regulation of PPS on the production of NO in peritoneal macrophages of mice may occur at transcriptional level of iNOS. This indicates that the mechanism of PPS's antitumor and immunoregulation functions may be related to increasing NO output of macrophages through stimulating iNOS's denovo synthesis.

Key words: polyporus polysaccharide, nitric oxide, nitric-oxide synthase, macrophages

CLC Number: 

  • R967

Tab 1

Effects of PPS on production of NO and activity of iNOS in peritoneal macrophages of mice ($\bar{x}$±s )"

Group n NO (μmol/L) iNOS (nmol/well/min)
PBS 6 3.65±0.59 1.84±0.11
PPS(1.0 mg/L) 6 6.79±0.44** 2.09±0.12
PPS(10 mg/L) 6 12.82±0.56** 6.51±0.15**
PPS(100 mg/L) 6 15.14±0.71** 7.36±0.12**
LPS(20 μg/L) 6 21.37±0.82** 9.75±0.82**

Fig 1

Effects of PPS on mRNA expression of iNOS in peritoneal macrophages of mice Ⅰ:DNA marker (200 bp ladder); Ⅱ:20 μg/L LPS; Ⅲ:100 mg/L PPS; Ⅳ:10 mg/L PPS; Ⅴ:1.0 mg/L PPS; Ⅵ:PBS"

Tab 2

Effects of PPS on mRNA expression of iNOSin peritoneal macrophages of mice"

Group n mRNA expression of iNOS (%)
PBS 3 2.4
PPS (1.0 mg/L) 3 25.3
PPS (10 mg/L) 3 63.8
PPS (100 mg/L) 3 82.5
LPS (20 μg/L) 3 100.0
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