猪苓多糖对小鼠腹腔巨噬细胞诱导型一氧化氮合酶活性及mRNA表达的影响

doi:10.3736/jcim20040512

Abstract

Objective: To study the mechanisms of the antitumor and immunoregulation functions of polyporus polysaccharide (PPS).Methods: The production of nitric oxide (NO), the activity and mRNA expression of inducible nitric oxide synthase (iNOS) in peritoneal macrophages of mice administered with different dose of PPS were observed by Griess reaction, fluorimetry assay and RT-PCR, respectively.Results: PPS could elevate the iNOS activity with dose-dependence and stimulate the iNOS mRNA expression of peritoneal macrophages in mice.Conclusion: The regulation of PPS on the production of NO in peritoneal macrophages of mice may occur at transcriptional level of iNOS. This indicates that the mechanism of PPS's antitumor and immunoregulation functions may be related to increasing NO output of macrophages through stimulating iNOS's denovo synthesis.

Key words: polyporus polysaccharide, nitric oxide, nitric-oxide synthase, macrophages

CLC Number:

R967

Di-nan Huang, Gan Hou, Qi-feng Zhu. Effects of polyporus polysaccharide on activity and mRNA expression of inducible nitric oxide synthase in peritoneal macrophages of mice. Journal of Chinese Integrative Medicine, 2004, 2(5): 350-352.

/ / Recommend

Add to citation manager EndNote| Reference Manager|ProCite|BibTeX|RefWorks

URL: http://www.jcimjournal.com/EN/10.3736/jcim20040512

http://www.jcimjournal.com/EN/Y2004/V2/I5/350

TrendMD

Figures/Tables 3

Tab 1

Fig 1

Tab 2

References 10

[1]	侯敢, 黄迪南, 祝其锋 . 猪苓多糖对巨噬细胞一氧化氮生成和细胞内还原型谷光甘肽的影响[J]. 上海免疫学杂志, 2001,21(2):98-99
[2]	Ding AH, Nathan CF, Stuehr DJ . Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages[J]. J Immunol, 1988,141(7):2407-2412
[3]	Wang W, Inoue N, Nakayama T , et al. An assay method for nitric oxide synthase in crude samples by determining product NADPg[J]. Anal Biochem, 1995,227(2):274-280 doi: 10.1006/abio.1995.1280
[4]	Friedl R, Brunner M, Moeslinger T , et al. Testosterone inhibits expression of inducible nitric oxide synthase in murine macrophages[J]. Life Sci, 2000,68(4):417-429 doi: 10.1016/S0024-3205(00)00953-X pmid: 11205891
[5]	Li Y, Ito N, Suzuki T , et al. Dexamathasone inhibits nitric oxide-mediated cytotoxicity via effects on both macrophages and target cells[J]. Immunopharmacology, 1995,30(2):177-186 doi: 10.1016/0162-3109(95)00018-O
[6]	Barnes PJ, Liew FY . Nitric-oxide and asthmatic inflammation[J]. Immunol Today, 1995,16(2):128-130 doi: 10.1016/0167-5699(95)80128-6 pmid: 7536418
[7]	Liu WK . Activation of peritoneal macrophages by polysaccharopeptide from the mushroom, coriolus versicolor[J]. Immunopharmacology, 1993,26(2):139-141 doi: 10.1016/0162-3109(93)90006-C
[8]	Lorsbach RB, Russel SW . A specific sequence of stimulation is required to induce synjournal of the antimicrobial molecule nitric oxide by mouse macrophages[J]. Infect Immun, 1992,60(5):2133-2135
[9]	Chang CI, Liao JC, Kuo L . Macrophage arginase promotes tumor cell growth and suppresses nitric oxide-mediated tumor cytotoxicity[J]. Cancer Res, 2001,61(3):1100-1106
[10]	Soini Y, Kalhos K, Puhakka , et al. Expression of inducible nitric oxide synthase in healthy pleura and in malignant mesothelioma[J]. Br J Cancer, 2000,83(7):880-886 doi: 10.1054/bjoc.2000.1384

Related Articles 15

[1]	Jia-yi Zhao, Jin Pu, Jian Fan, Xin-yu Feng, Jian-wen Xu, Rong Zhang, Yan Shang. Tanshinone IIA prevents acute lung injury by regulating macrophage polarization. Journal of Integrative Medicine, 2022, 20(3): 274-280.
[2]	Swatantra Kumar, Vimal K Maurya, Debadatta Nayak, Anil Khurana, Raj K Manchanda, Srinivasulu Gadugu, Madan LB Bhatt, Shailendra K Saxena. Calcarea carbonica treatment rescues lipopolysaccharide-induced inflammatory response in human mononuclear cells via downregulation of inducible cyclooxygenase pathway. Journal of Integrative Medicine, 2020, 18(5): 441-449.
[3]	Bikash Manandhar, Hyun Jung Kim, Dong Young Rhyu. Caulerpa okamurae extract attenuates inflammatory interaction, regulates glucose metabolism and increases insulin sensitivity in 3T3-L1 adipocytes and RAW 264.7 macrophages. Journal of Integrative Medicine, 2020, 18(3): 253-264.
[4]	The Su Moe, Htet Htet Win, Thin Thin Hlaing, War War Lwin, Zaw Min Htet, Khin Mar Mya . Evaluation of in vitro antioxidant, antiglycation and antimicrobial potential of indigenous Myanmar medicinal plants. Journal of Integrative Medicine, 2018, 16(5): 358-366.
[5]	Byung Hyuk Han, Yun Jung Lee, Jung Joo Yoon, Eun Sik Choi, Seung Namgung, Xian Jun Jin, Da Hye Jeong, Dae Gill Kang, Ho Sub Lee. Hwangryunhaedoktang exerts anti-inflammation on LPS-induced NO production by suppressing MAPK and NF-κB activation in RAW264.7 macrophages. Journal of Integrative Medicine, 2017, 15(4): 326-336.
[6]	Omowumi Titilola Kayode, Musa Toyin Yakubu. Parquetina nigrescens leaves: chemical profile and influence on the physical and biochemical indices of sexual activity of male Wistar rats. Journal of Integrative Medicine, 2017, 15(1): 64-76.
[7]	Rajendra Karki, Cheol-Ho Park, Dong-Wook Kim. Extract of buckwheat sprouts scavenges oxidation and inhibits pro-inflammatory mediators in lipopolysaccharide-stimulated macrophages (RAW264.7). Journal of Chinese Integrative Medicine, 2013, 11(4): 246-252.
[8]	Ju-yong Wang, Jing Guo, Chang-sheng Dong, Zhan Zheng, Qing Wang. Effects of Feiyanning Decoction, a compound traditional Chinese medicine, on iNOS and COX-2 expressions induced by tumor necrosis factor-α in lung adenocarcinoma cell line. Journal of Chinese Integrative Medicine, 2012, 10(6): 695-700.
[9]	Tapas Kumar Sur, Biswajit Auddy, Dipankar Bhattacharyya. Effects of Indian herbal formulation Body Revival on human platelet aggregation and myocardial ischemia in rats. Journal of Chinese Integrative Medicine, 2011, 9(7): 746-751.
[10]	Bamidele Victor Owoyele, Omosunkanmi Toyin Adenekan, Ayodele Olufemi Soladoye. Effects of honey on inflammation and nitric oxide production in Wistar rats. Journal of Chinese Integrative Medicine, 2011, 9(4): 447-452.
[11]	Zhao Li, Liang Xiao-chun, Zhang Hong, Wang Pu-yan, Sun Qing, Huang Wen-zhi, Li Bo-wu. Effects of Chinese herbal medicine Jinmaitong-medicated serum on inducible nitric oxide synthase and NADPH oxidase p22-phox subunit of rat Schwann cells cultured in high-glucose medium. Journal of Chinese Integrative Medicine, 2011, 9(3): 299-305.
[12]	Gollapalle L. Viswanatha, Hanumanthappa Shylaja, K. Sadashiva Sandeep Rao, Yathiraj Ashwini, V. Ramaiah Santhosh Kumar, C. Gangadharaiah Mohan, Venkate Gowda Sunil, M. Venkateshappa Sarvesh Kumar, Subbanna Rajesh. Amelioration of immobilization stress-induced biochemical and behavioral alterations and mitochondrial dysfunction by naringin in mice: Possible mechanism of nitric oxide modulation. Journal of Chinese Integrative Medicine, 2011, 9(11): 1254-1263.
[13]	Qing Lin , Jian Zheng, Si Ai, Li-xing Lin, Yu-xin Chen, Fang Wang, Ling Zhu . Mechanism of combined treatment with Shenkangling Decoction and prednisone in rats with adriamycin-induced nephropathy. Journal of Chinese Integrative Medicine, 2009, 7(7): 661-666.
[14]	Qin Zhang, Yun-mei Yang, Guo-you Yu. Effects of gastrodin injection on blood pressure and vasoactive substances in treatment of old patients with refractory hypertension: a randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(7): 695-699.
[15]	Xiao-lei Hou, Bai-ling Li, Lei Zhao, Sheng-dong Huang, Zhi-yun Xu, Guan-xin Zhang. Effects of Xuefu Zhuyu Capsule on endothelin-1 release in myocardium and vascular endothelium and nitric oxide/nitric oxide synthase system of swines after acute myocardial infarction and reperfusion. Journal of Chinese Integrative Medicine, 2008, 6(4): 381-386.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 10

[1]	Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2]	Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3]	Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4]	Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5]	. Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6]	Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7]	Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8]	Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9]	Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10]	Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228

Group	n	NO （μmol/L）	iNOS (nmol/well/min)
PBS	6	3.65±0.59	1.84±0.11
PPS(1.0 mg/L)	6	6.79±0.44**	2.09±0.12
PPS(10 mg/L)	6	12.82±0.56**	6.51±0.15**
PPS(100 mg/L)	6	15.14±0.71**	7.36±0.12**
LPS(20 μg/L)	6	21.37±0.82**	9.75±0.82**

Group	n	mRNA expression of iNOS （%）
PBS	3	2.4
PPS (1.0 mg/L)	3	25.3
PPS (10 mg/L)	3	63.8
PPS (100 mg/L)	3	82.5
LPS (20 μg/L)	3	100.0

Effects of polyporus polysaccharide on activity and mRNA expression of inducible nitric oxide synthase in peritoneal macrophages of mice

RichHTML

PDF (PC)

Cited

Like