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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (11): 1073-1077.doi: 10.3736/jcim20091110

• Original Experimental Research • Previous Articles     Next Articles

Effects of Chaihu Shugan San on behavior and plasma levels of corticotropin releasing hormone and adrenocorticotropic hormone of rats with chronic mild unpredicted stress depression

Yun-hui Lia,Chun-hu Zhanga,Su-e Wanga,Juan Qiub,Sui-yu Hua,Gui-lin Xiaoa   

  1. a Institute of Integrative Medicine, Key Unit of State Administration of Traditional Chinese Medicine of the Peoples 5 s Republic of China, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China
    b Department of Nuclear Medicine, Xiangya 3rd Hospital, Central South University, Changsha 410013, Hunan Province, China
  • Received:2009-07-20 Accepted:2009-09-15 Online:2009-11-20 Published:2009-11-15
  • Contact: Chun-hu Zhang E-mail:chzxysm@yahoo.com

Objective

To investigate the effects of Chaihu Shugan San (CHSGS), a compound traditional Chinese herbal medicine, on behavior and plasma levels of corticotropin releasing hormone (CRH) and adrenocorticotropic hormone (ACTH) of rats with chronic mild unpredicted stress depression.
Methods

Forty male Sprague-Dawley rats were randomly divided into 4 groups: normal control group, untreated group, fluoxetine group and CHSGS group. Except the normal control group, rats were singly housed and exposed to an unpredicted sequence of mild stressor for continuous 4 weeks to induce depression. Since the fifteenth day, rats were intragastrically administered with equal volume agents respectively for 2 weeks [normal saline for the normal control group and the untreated group, fluoxetine (1.8 mg/kg) for the fluoxetine group and CHSGS (5.9 g/kg) for the CHSGS group]. Behavioral scores of rats were detected by open-field test and sucrose preference test, and the plasma levels of CRH and ACTH in different groups were detected by radioimmunoassay.
Results

 Compared with the normal control group, body weights of the rats in the untreated group were significantly decreased. Scores of crossing, rears and grooming in open-field test were reduced significantly. Pure water consumption in sucrose preference test was increased significantly. The levels of plasma CRH and ACTH were significantly increased. The depressive behaviors of the rats were improved significantly and the levels of plasma CRH and ACTH were obviously reduced in the CHSGS group.
Conclusion

Chronic mild unpredicted mild stress can affect the neuroendocrine and behavior and cause depression in rats. CHSGS can regulate HPA hyperactivity of rats caused by chronic stress and has antidepressive effects.

Key words: Chaihu Shugan San, Depression, Chronic stress, Neurobehavioral manifestations, Corticotropin releasing hormone, Adrenocorticotropic hormone, Rats

Table 1

Behaviors and body weight before duplicating model and pretherapy and post-therapy in rats ($\bar{x}$±s)"

Group n Scores
of crossing
Scores
of rears
Time of staying
in central square
Time of
grooming
Total water
consumption
(mL)
1% sucrose
consumption
(mL)
Weight (g)
Normal control
Day 0 10 62.9±14.2 16.1±6.1 0.0±0.0 7.5±3.3 16.0±3.9 13.2±4.2 202.8±10.1
Day 15 10 63.4±13.2 15.1±6.4 0.1±0.3 7.7±3.8 15.1±4.3 13.3±3.8 270.5±15.6
Day 29 10 61.3±18.8 15.0±5.9 0.0±0.0 8.5±3.4 19.3±5.3 16.4±4.7 328.9±22.9
Untreated
Day 0 10 65.8±13.6 15.3±4.3 0.0±0.0 7.8±4.0 16.1±4.6 14.7±3.4 205.2±11.6
Day 15 10 26.4±9.6** 4.3±2.1** 3.2±1.8** 3.4±1.1** 15.5±3.4** 6.8±2.4** 251.5±18.7*
Day 29 10 19.3±7.2** 6.9±2.9** 4.6±2.6** 2.6±1.8** 11.1±1.9** 7.3±2.5** 274.7±15.1**
Fluoxetine
Day 0 10 66.0±16.1 15.7±7.2 0.0±0.0 7.3±3.3 14.8±3.9 12.5±4.1 206.7±14.4
Day 15 10 24.8±10.3** 5.2±2.9** 3.6±1.7** 3.7±2.2** 13.7±3.3** 8.1±2.9** 248.6±15.6*
Day 29 10 46.3±18.3△△ 12.2±6.1△△ 1.4±0.7△△ 5.9±2.4△△ 18.5±5.4△△ 13.5±3.9△△ 306.5±25.8△△
GHSGS
Day 0 10 63.5±16.6 15.7±5.6 0.0±0.0 7.1±3.2 16.6±3.8 12.4±3.2 201.9±12.1
Day 15 10 25.8±12.4** 4.5±2.5** 3.5±2.1** 3.1±1.9** 15.6±4.5** 8.2±1.8** 247.7±14.3*
Day 29 10 45.2±18.1 11.7±4.3△△ 1.1±0.7△△ 5.7±3.0△△ 19.2±4.8△△ 14.1±3.0△△ 309.2±28.5△△

Table 2

Comparison of the levels of CRH and ACTH in plasma in post-treatment rats ($\bar{x}$±s)"

Group n CRH (ng/mL) ACTH (ng/L)
Normal control 10 6.40±1.96 50.79±11.41
Untreated 10 20.29±6.25** 82.66±13.51**
Fluoxetine 10 8.88±3.39△△ 53.31±13.91△△
CHSGS 10 8.29±2.41△△ 54.14±14.07△△
[1] Duman RS, Kehne JH . Depression[J]. CNS Neurol Disord Drug Targets, 2007,6(3):161-162
doi: 10.2174/187152707780619317
[2] Hu SY, Zhang HG, Zheng L, Zhang HN, Chen ZQ, Chen CH, Zhao ZF, Wang XB, Liu FR, Li SM, Huang XC, Feng JL, Zhang J . Analysis of TCM different syndromes composition ratio in 1 977 patients with depression[J]. Zhongguo Yi Shi Za Zhi, 2003,5(10):1312-1314
胡随瑜, 张宏耕, 郑林, 张海男, 陈泽奇, 陈昌华, 赵志付, 王新本, 刘发荣, 李顺民, 黄信初, 俸建林, 张捷 . 1 977例抑郁症患者中医不同证候构成比分析[J]. 中国医师杂志, 2003,5(10):1312-1314
[3] He XM, Zhang YK . Clinical investigation of Chaihu Shugan San combined with fluoxetine for treatment of 36 patients with poststroke depression[J]. Xin Zhong Yi, 2007,39(10):22-23
doi: 10.3969/j.issn.0256-7415.2007.10.012
贺新民, 张益康 . 柴胡疏肝散加味联合百忧解治疗中风后抑郁症36例疗效观察[J]. 新中医, 2007,39(10):22-23
doi: 10.3969/j.issn.0256-7415.2007.10.012
[4] Yan HX, Ren F, Gu J . Effects of Chaihu Shugan San on rats of liver qi depression[J]. Zhong Yao Yao Li Yu Lin Chuang, 2006,22(6):5-6
doi: 10.3969/j.issn.1001-859X.2006.06.003
严亨秀, 任昉, 顾健 . 柴胡疏肝散对实验性肝郁证大鼠的影响[J]. 中药药理与临床, 2006,22(6):5-6
doi: 10.3969/j.issn.1001-859X.2006.06.003
[5] Szymańska M, Budziszewska B, Jaworska-Feil L, Basta-Kaim A, Kubera M, Leskiewicz M, Regulska M, Lasoń W . The effect of antidepressant drugs on the HPA axis activity, glucocorticoid receptor level and FKBP51 concentration in prenatally stressed rats[J]. Psychoneuroendocrinology, 2009,34(6):822-832
doi: 10.1016/j.psyneuen.2008.12.012
[6] Benelli A, Filaferro M, Bertolini A, Genedani S . Influence of sadenosly-1-methionine on chronic mild stress-induced anhedonia in castrated rats[J]. Br J Pharmacol, 1999,127(3):645-654
doi: 10.1038/sj.bjp.0702589
[7] Gronli J, Bramham C, Murison R, Kanhema T, Fiske E, Bjorvatn B, Ursin R, Portas CM . Chronic mild stress inhibits BDNF protein expression and CREB activation in the dentate gyrus but not in the hippocampus proper[J]. Pharmacol Biochem Behav, 2006,85(4):842-849
doi: 10.1016/j.pbb.2006.11.021
[8] Papp M, Nalepa I, Antkiewicz-Michaluk L, Sanchez C . Behavioural and biochemical studies of citalopram and WAY 100635 in rat chronic mild stress model[J]. Pharmacol Biochem Behav, 2002,72(1-2):465-474
doi: 10.1016/S0091-3057(01)00778-X
[9] Wei ZJ . Chaihu Shugan powder plus psychotherapy for depressive disorder: observation of 35 cases[J]. Zhongguo Yi Yuan Yong Yao Ping Jia Yu Fen Xi, 2007,7(5):379-380
尉志军 . 柴胡疏肝散加味配合心理治疗抑郁症35例[J]. 中国医院用药评价与分析, 2007,7(5):379-380
[10] Hu SY, Zhang CH, Cao MQ, Peng GJ, Huang CY . Anti-depressive effect of Baisong tablets on the chronic mild unpredicted stress depression in rats[J]. Zhong Nan Da Xue Xue Bao Yi Xue Ban, 2006,31(5):676-681
doi: 10.3321/j.issn:1672-7347.2006.05.013
胡随瑜, 张春虎, 曹美群, 彭贵军, 黄川原 . 白松片对大鼠慢性应激抑郁模型的抗抑郁实验研究[J]. 中南大学学报(医学版), 2006,31(5):676-681
doi: 10.3321/j.issn:1672-7347.2006.05.013
[11] Meyer U, van Kampen M, Isovich E, Flügge G, Fuchs E . Chronic psychosocial stress regulates the expression of both GR and MR mRNA in the hippocampal formation of tree shrews[J]. Hippocampus, 2001,11(3):329-336
doi: 10.1002/hipo.1047
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