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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (1): 35-38.doi: 10.3736/jcim20050111

• Original Experimental Research • Previous Articles     Next Articles

Effects of Danshen Decoction on experimental gastric ulcer in rats and mice

Li Liu,Hong-quan Zhang   

  1. Medical and Pharmaceutical Institute, Yangzhou University, Yangzhou, Jiangsu Province 225001, China
  • Received:2004-07-06 Online:2005-01-20 Published:2005-01-20
  • Contact: Hong-quan Zhang E-mail:xzyxywyq@sina.com

Objective

To investigate the preventive and therapeutic effects of Danshen Decoction on gastric ulcer in experimental animal models.

Methods

The model of water immersion restraint stress ulcer in mice, and the models of acetic acid impaired gastric ulcer and pyloric ligation gastric ulcer in rats were established. The total gastric acid, the activity of pepsin and the amount of gastric wall binding mucus were detected.

Results

Danshen Decoction reduced the acute gastric mucosal lesion. The effect of 5.0 or 10.0 g/kg group was obviously better than that of the normal saline control group (P<0.05 and P<0.01 respectively). Danshen Decoction accelerated the healing of ulcer induced by acetic acid significantly, and increased the content of gastric wall binding mucus (P<0.01); and it also inhibited the formation of gastric ulcer induced by pyloric ligation, while having no influence on the secretion of gastric acid and the activity of pepsin.

Conclusion

Danshen Decoction has a significant anti-ulcerative effect, which may be related to improving the gastric mucosal defensive function.

Key words: Danshen Decoction, Stomach ulcer, Animals, Laboratory

CLC Number: 

  • R289.11

Tab 1

Effect of Danshen Decoction on water immersion restraint stress ulcer in mice ($\bar{x}$±s)"

Group n Ulcer index
Normal saline 10 17.60±4.27
Ranitidine (0.04 g/kg) 10 9.90±2.13**
Danshen Decoction (2.5 g/kg) 10 13.60±4.70
Danshen Decoction (5.0 g/kg) 10 10.90±4.68*
Danshen Decoction (10.0 g/kg) 10 8.80±3.33**

Tab 2

Effect of Danshen Decoction on gastric ulcer induced by acetic acid in rats ($\bar{x}$±s)"

Group n Ulcer index (mm2)
Normal saline 8 7.88±2.53
Ranitidine (0.03 g/kg) 8 2.93±0.88**
Danshen Decoction (1.75 g/kg) 8 3.92±1.81**
Danshen Decoction (3.5 g/kg) 8 3.05±0.67**
Danshen Decoction (7.0 g/kg) 8 2.04±0.94**

Tab 3

Effect of Danshen Decoction on gastric ulcer induced by pyloric ligation in rats ($\bar{x}$±s)"

Group n Ulcer index
Normal saline 8 4.13±1.36
Ranitidine (0.03 g/kg) 8 1.75±0.89**
Danshen Decoction (1.75 g/kg) 8 3.38±1.19△△
Danshen Decoction (3.5 g/kg) 8 2.50±1.31*
Danshen Decoction (7.0 g/kg) 8 2.38±1.51*

Tab 4

Effects of Danshen Decoction on secretion of gastric acid and activity of pepsin in pyloric ligation gastric ulcer in rats ($\bar{x}$±s)"

Group n Gastric acid (mmol/L) Pepsin (kU/L)
Normal saline 8 8.12±1.55 424.5±60.4
Ranitidine (0.03 g/kg) 8 3.47±2.18** 322.4±65.5*
Danshen Decoction (1.75 g/kg) 8 7.22±1.55△△ 362.8±55.0
Danshen Decoction (3.5 g/kg) 8 6.49±2.29 368.8±67.9
Danshen Decoction (7.0 g/kg) 8 6.34±2.97 396.6±64.3

Tab 5

Effect of Danshen Decoction on amount of gastric wall binding mucus in acetic acid-induced gastric ulcer in rats ($\bar{x}$±s)"

Group n PAS positive area (%)
Normal saline 8 9.61±2.02
Ranitidine (0.03 g/kg) 8 12.26±2.67*
Danshen Decoction (1.75 g/kg) 8 14.16±3.17**
Danshen Decoction (3.5 g/kg) 8 15.34±2.14**
Danshen Decoction (7.0 g/kg) 8 18.94±2.90**△△

Fig 1

Effect of Danshen Decoction on amount of gastric wall binding mucus in acetic acid-induced gastric ulcer (AB-PAS×200) A: Normal saline group; B: Ranitidine group; C: Danshen Decoction (3.5 g/kg) group; D: Danshen Decoction (7.0 g/kg) group"

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