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Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (5): 375-378.doi: 10.3736/jcim20040519

• Technique and Methods • Previous Articles     Next Articles

Osteogenesis characteristics of cultured rat mesenchymal stem cells under bone induction condition

Dong-feng Chen1(), Hui Li1, Jian-hong Zhou1, Yao Xie2, Yi-wei Li1, Shao-hui Du3, Yi Zhang4, Jie Huang5, Min Xu6   

  1. 1. Department of Anatomy , Guangzhou University of Traditional Chinese Medicine , Guangzhou , Guangdong Province 510405 , China
    2. Department of Anatomy , Zhongshan Medical College, SUN Yat-sen University , Guangzhou , Guangdong Province 510080 ,China
    3. Department of Internal Medicine, Shenzhen Hospital of Traditional Chinese Medicine , Shenzhen ,Guangdong Province 518000 , China
    4. Shenzhen Shahe Hospital , Shenzhen , Guangdong Province 518000 , China
    5. Shenzhen People’s Hos pital , Shenzhen , Guangdong Province 518000 , China ;
    6. School of Traditional Chinese Medicine , HongKong Baptise Univesity , Hong Kong , China
  • Received:2003-09-20 Online:2004-09-20 Published:2018-10-20

Objective: To investigate the osteogenesis characteristics of cultured rat mesenchymal stem cells (MSCs) under bone induction condition.Methods: MSCs were isolated from adult rat by using density gradient separation method. The osteogenic inducers were compounds of Dexone, β-glycerophosphate sodium and vitamin C.Results: The MSC attachment formed soon after the seeding and grew into colonies with the appearance of fibroblastic cells. The osteogenic inducer with low dose of Dexone could promote the osteogenic differentiation of MSC.In the group of osteogenic inducer with low dose of Dexone, the expression of alkaline phosphatase (ALP) was remarkably increased after one week’s induction, and the number of positive cells was (15.1±2.6), significantly higher than that of the control group (12.0±3.5) (P<0.01). The calcified deposits began to appear in the group of osteogenic inducer with low dose of Dexone after one week's induction and was increased remarkably after three weeks, and the number of calcified deposits was (9.0±1.7), significantly higher than that of the control group (2.0±1.8) (P<0.01).Conclusion: MSC can differentiate into osteogenesis by osteogenic induction and may be used to provide seed cells for bone tissue engineering.

Key words: stem cell myeloid, osteoblasts, animal, laboratory

CLC Number: 

  • R329.471

Fig 1

Primary MSCs culture with appearance of colony-forming units-fibroblast (×200)"

Fig 2

Passage 1 MSCs showing fibroblast-like array (×100)"

Tab 1

Number of ALP positive cells after induction($\bar{x}$±s,cells)"

Group n 7 d 14 d 21 d 28 d
Control 20 12.0±3.5 13.3±3.2 14.6±3.9 4.5±3.1
Low dose 20 15.1±2.6** 16.6±2.3** 18.4±2.2** 19.4±2.5**
High dose 20 13.2±1.9 14.7±2.5 15.7±2.6 16.1±2.3

Fig 3

ALP positive cells in passage 1 MSCs after 7 d low dose Dexone induction (×200)"

Tab 2

Number of calcified deposits after induction($\bar{x}$±s,deposits)"

Group n 7 d 14 d 21 d 28 d
Control 10 0 2.1±1.4 2.0±1.8 3.5±1.7
Low dose 10 4.3±1.6** 7.6±1.6** 9.0±1.7** 9.3±1.4**
High dose 10 0 1.9±1.3 3.5±1.4 4.7±1.5

Fig 4

Calcified deposits in passage 1 MSCs after 7d low dose Dexone induction (×200)"

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