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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (5): 370-373.doi: 10.3736/jcim20050510

• Original Experimental Research • Previous Articles     Next Articles

Effects of recipes for replenishing qi and activating blood on senescence related gene expressions in the liver of aging rats

Zhi-ping Li1,Wei-kang Zhao2,Pin-chu Xu2,Fang-min Chen2,Guo-qin Jin1,Ming-zhong Yao2   

  1. 1. Department of Biochemistry, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    2. Institute of Geriatrics, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2004-11-26 Online:2005-09-20 Published:2005-09-20
  • Contact: Wei-kang Zhao


To observe the effects of recipes for replenishing qi and activating blood on p16, p21, proliferating cell nuclear antigen (PCNA), cyclin D1 and cyclin E gene expressions in the liver of aging rats.


A recipe for replenishing qi and a recipe for activating blood were administered to aging rats respectively, and the effects of the above recipes on the expressions of senescence related genes (p16, p21, PCNA, cyclin D1 and cyclin E) were examined by RT-PCR and Western blotting methods.


The expressions of p16, p21 and cyclin D1 mRNAs and proteins in the liver of the untreated aging rats were up-regulated, while the expressions of PCNA and cyclin E mRNAs and proteins decreased. As compared with the untreated aging rats, both recipes could down-regulate the expressions of cyclin D1 mRNA and protein and up-regulate the expressions of cyclin E mRNA and protein, but had no obvious effects on the expressions of mRNAs and proteins of p16, p21 and PCNA.


Recipes for replenishing qi and activating blood can improve the liver cell proliferation of aging rats via down-regulating the expressions of cyclin D1 mRNA and protein and up-regulating the expressions of cyclin E mRNA and protein.

Key words: Aging rats, Hepatocytes, p16, p21, Proliferating cell nuclear antigen, Cyclin D1, Cyclin E, Qi-benefiting recipe, Blood-activating recipe

CLC Number: 

  • R339.38

Fig 1

Agarose gel electrophoresis of p16 and p21 M: Marker; 1:Young control group; 2: Untreated group; 3: Qi-benefiting recipe-treated group; 4: Blood-activating recipe treated group"

Fig 2

Agarose gel electrophoresis of cyclin D1, PCNA and cyclin E M: Marker; 1: Young control group; 2: Untreated group; 3: Qi-benefiting recipe-treated group; 4: Blood-activating recipe-treated group"

Tab 1

Effects of qi-benefiting and blood-activating recipes on mRNA expressions of p16, p21, PCNA, cyclin D1 and cyclin E genes in the liver of rats (target gene/β-actin) ($\bar{x}$±s, %)"

Group n p16 p21 Cyclin D1 PCNA Cyclin E
Young control 6 0.83±0.28 0.81±0.40 0.71±0.07 1.72±0.47 1.02±0.07
Untreated 6 1.33±0.26** 1.43±0.38** 1.40±0.28* 0.85±0.21** 0.48±0.08**
Qi-benefiting recipe-treated 6 1.08±0.27 1.22±0.23 0.92±0.16△△ 1.24±0.43 0.70±0.13△△
Blood-activating recipe-treated 6 1.13±0.29 1.26±0.39 0.88±0.07△△ 1.16±0.31 0.74±0.14△△

Tab 2

Effects of qi-benefiting and blood-activating recipes on protein expressions of p16,p21 PCNA,cyclin D1 and cyclin E genes in the liver of rats (target gene/β-actin) ($\bar{x}$±s, %)"

Group n p16 p21 Cyclin D1 PCNA Cyclin E
Young control 6 0.50±0.08 0.54±0.16 0.45±0.04 2.30±0.37 1.69±0.13
Untreated 6 1.67±0.43** 1.68±0.35** 2.30±0.45** 0.78±0.228** 0.70±0.13**
Qi-benefiting recipe-treated 6 1.45±0.36 1.54±0.41 1.28±0.28△△ 1.09±0.29 1.04±0.12△△
Blood-activating recipe-treated 6 1.49±0.49 1.33±0.26 1.05±0.24△△ 1.06±0.29 1.08±0.22△△

Fig 3

Western blotting results of p16, p21, PCNA, cyclin D1 and cyclin E genes 1:Young control group; 2: Untreated group; 3: Qi-benefiting recipe-treated group; 4: Blood-activating recipe-treated group"

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