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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (4): 432-436.doi: 10.3736/jcim20070414

• Original Experimental Research • Previous Articles     Next Articles

Effect of Xiaotan Sanjie Recipe on growth of transplanted tumor and expressions of proliferating cell nuclear antigen and epidermal growth factor receptor in tissue of gastric carcinoma of nude mice

Xiao-dong Guo1, Pin-kang Wei2   

  1. 1. Department of Oncology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
    2. Department of Traditional Chinese Medicine, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
  • Online:2007-07-31 Published:2007-05-15

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Objective: To observe the influence of Xiaotan Sanjie Recipe on growth of the transplanted tumor and expressions of proliferating cell nuclear antigen (PCNA) and epidermal growth factor receptor (EGFR) in tissue of the gastric carcinoma of nude mice, and to explore the mechanism of Xiaotan Sanjie Recipe in restraining the multiplication of the stomach cancer.Methods: MKN-45 gastric carcinoma model in nude mice was established. Forty-five nude mice were randomly divided into control group, Xiaotan Sanjie group and 5-fluorouracil (5-FU) group. Drugs were given on the next day of innoculation. Mice in the control group were given normal saline, and mice in the Xiaotan Sanjie group were given Xiaotan Sanjie Recipe. Intraperitoneal injection of 5-FU was administered in the 5-FU group. The expressions of PCNA and EGFR were examined by using streptavidin peroxidase (SP) conjugate technique, and the tumor weight and pathological characteristics were also observed.Results: Compared with the control group, Xiaotan Sanjie Recipe significantly restrained the growth of the transplanted tumor, and reduced the expressions of PCNA and EGFR in tissue of the gastric carcinoma of nude mice (P＜0.05).Conclusion: Xiaotan Sanjie Recipe can disturb the synthesis of DNA and reduce the proliferous activity of cancer cells by decreasing the expressions of PCNA and EGFR.

Key words: stomach neoplasms, Chinese herbal drugs, proliferating cell nuclear antigen, epidermal growth factor receptor, nude mice

CLC Number: 

  • R735.2

Table 1

Tumor weight and inhibition rate in different groups"

Group n Tumor weight (x±s, mg) Inhibition rate (%)
Control 15 949.3±271.2
Xiaotan Sanjie Recipe 15 448.8±189.6* 52.72
5-FU 14 324.7±106.8** 65.71

Figure 1

Pathological observation of tumor tissue (HE, ×200)A: Control group; B: Xiaotan Sanjie Recipe group; C: 5-FU group."

Table 2

Positive expression of PCNA in different groups (±s, %)"

Group n Positive expression rate
Control 15 54.33±20.10
Xiaotan Sanjie Recipe 15 18.66±8.74*
5-FU 14 11.94±2.65**

Figure 2

Positive expression of PCNA in different groups (SP, ×200)A: Control group; B: Xiaotan Sanjie Recipe group; C: 5-FU group."

Figure 3

Positive expression of EGFR in different groups (SP, ×200)A: Control group; B: Xiaotan Sanjie Recipe group; C: 5-FU group."

Table 3

Positive expression of EGFR in different groups"

Group n Positive Negative Positive rate (%)
Control 15 12 3 80.0
Xiaotan Sanjie Recipe 15 6 9 40.0*
5-FU 14 4 10 28.6**
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