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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (8): 836-842.doi: 10.3736/jcim20080813

• Original Experimental Research • Previous Articles     Next Articles

Effects of Buyang Huanwu Decoction and its alkaloids and glycosides on aortic intimal hyperplasia and expression of proliferating cell nuclear antigen in rats with aortic intimal injuries

Lu Wu, Wei Zhang, Hua Li, Guo-min Zhang, Bei-yang Chen, Ying-hong Tang, Chang-qing Deng()   

  1. Laboratory of Pathophysiology, Hunan University of Traditional Chinese Medicine, Changsha, Hunan Province 410007, China
  • Received:2007-12-26 Online:2008-08-20 Published:2008-08-15

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Objective: To explore the effects of alkaloids and glycosides extracted from Buyang Huanwu Decoction (BYHWD), a compound of traditional Chinese herbal medicine, on aortic intimal hyperplasia and the expression of the proliferating cell nuclear antigen (PCNA) in rats with aortic intimal injuries.
Methods: The vessel restenosis model was established by denuding aortic endothelium with domestic balloon catheter in rats. Drugs were administered intragastrically on the first day after operation. The injured segments of aorta were taken on the fifteenth day after operation to determine the degree of intimal hyperplasia and observe the expression of PCNA.
Results: Aortic intimal hyperplasias were very obvious on the fifteenth day after operation. The media hyperplasias in the drug-treated groups were not significant (P>0.05),but the intimal hyperplasia were remarkable as compared with that in the sham-operated group (P<0.01). The degrees of intimal hyperplasia in BYHWD, alkaloids, glycosides and atorvastatin groups were less than that in the untreated group (P<0.01). There was significant difference of PCNA expression between the untreated group and the sham-operated group (P<0.01). The expressions of PCNA in alkaloids, glycosides, and atorvastatin groups were higher than that in the sham-operated group (P<0.01, P<0.05), but still lower than that in the untreated group (P<0.01). The expression of PCNA in BYHWD group was higher than that in the sham-operated group (P<0.01), and no significant difference was found between the BYHWD group and the untreated group (P>0.05).
Conclusion: Alkaloids and glycosides extracted from BYHWD can inhibit intimal hyperplasia induced by denuding arterial endothelium with domestic balloon catheter in rats. Alkaloids and glycoside may be among basal substances in BYHWD inhibiting intimal hyperplasia of blood vessel, the effect of which may relate to down-regulating the expression of PCNA.

Key words: Buyang Huanwu Decoction, alkaloids, glycosides, balloon catheter, vascular smooth muscle cell, morphology, proliferating cell nuclear antigen

CLC Number: 

  • R285.5

Figure 1

HPLC determination spectra of BYHWD integral prescription and alkaloids, glycosides extracted from BYHWD A-1: Tetramethylpyrazine; A-2: Alkaloids; A-3: BYHWD. B-1: Astragaloside Ⅳ; B-2: Ferulic acid, amygdalin, paeoniflorin; B-3: Glycosides; B-4: BYHWD."

Figure 2

Vascular morphology of rats in different groups (Light microscopy, ×100) A: Sham-operated group; B: Untreated group; C: Atorvastatin group; D: BYHWD group; E: Alkaloids group; F: Glycosides group. Arrows indicate the region of intimal hyperplasia."

Table 1

Measurement parameters of vascular morphology and expressions of PCNA of rats in different groups（x±s）"

Group n MA (μm2)I A (μm2) IA/MA IA/MA MT (μm)
Sham-operated 10 9 984.30±657.00 0.00±0.00 0.00±0.00 12.24±0.95
Untreated 10 10 705.50±1 716.86 4 173.75±1 804.88** 0.40±0.18** 12.08±1.64
Atorvastatin 10 11 281.85±1 167.10 2 369.25±788.19**△△ 0.21±0.07**△△ 12.97±1.48
BYHWD 10 11 790.45±1 311.26 2 147.80±916.12**△△ 0.18±0.07**△△ 13.33±1.57
Alkaloids 10 12 028.10±1 860.75 1 900.20±572.66**△△ 0.16±0.06**△△ 13.05±1.40
Glycosides 10 11 755.80±1 254.00 1 934.80±735.82**△△ 0.17±0.07**△△ 13.50±1.98
Group n IT/MT HRIA (％) HRIT (％) PCNA IT (μm)
Sham-operated 10 0.00±0.00 0 0 161.4±22.3 0.00±0.00
Untreated 10 0.42±0.19** 27±9** 29±10** 113.7±12.7** 5.01±2.11**
Atorvastatin 10 0.22±0.07**△△ 17±5**△△ 18±5**△△ 136.1±7.2**△△ 2.92±1.07**△△
BYHWD 10 0.19±0.07**△△ 15±5**△△ 16±5**△△ 124.4±18.8** 2.55±1.11**△△
Alkaloids 10 0.17±0.06**△△ 14±4**△△ 14±5**△△ 144.7±17.4*△△ 2.22±0.77**△△
Glycosides 10 0.18±0.07**△△ 14±5**△△ 15±5**△△ 134.8±5.7**△△ 2.36±0.90**△△

Figure 3

PCNA expressions in intima of rats in different groups by immunohistochemistry (Light microscopy, ×400) A: Sham-operated group; B: Untreated group; C: Atorvastatin group; D: BYHWD group; E: Alkaloids group; F: Glycosides group. Arrows indicate the region of intimal hyperplasia."

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