健脾中药复方胃肠安对人胃癌裸小鼠原位移植瘤生长和转移的影响

doi:10.3736/jcim20050512

Abstract

Objective

To evaluate the growth-inhibiting and anti-metastasis effects of Weichang'an Decoction (WCAD) on orthotopic transplant nude mouse model of human gastric cancer.

Methods

Forty-one nude mice were implanted with SGC-7901 cells at orthotopic site, whereas 25 were implanted with SGC-7901 cells subcutaneously. Then the nude mice in each transplantation model were divided into the same three groups which were WCAD-treated group with WCAD 0.5 ml/d taken orally, 5-FU-treated group with 5-FU 50 mg/kg intraperitoneally injected weekly and untreated control group with physiological saline 0.5 ml/d taken orally. The growth-inhibiting rates of transplant tumors were detected and the metastatic lesions were examined in the orthotopic transplant mouse model while PCNA-positive rate and apoptotic index (AI) were observed in the subcutaneous transplant mouse model.

Results

The growth-inhibiting rates in the WCAD-treated and 5-FU-treated groups of orthotopic transplant mouse model were 40.82% and 37.92% respectively whereas those of subcutaneous transplant mouse model were 48.70% and 60.10%. The incidence rates of metastasis in perigastric lymph notes, lymph nodes in the porta hepatis, liver, diaphragm and peritoneum in the WCAD-treated and 5-FU-treated groups were lower than those in the untreated control group, and the total metastasis rates in the WCAD-treated, 5-FU-treated and the untreated control groups were 30.77%, 28.57% and 71.43% respectively with significant differences (P<0.05). The total PCNA-positive rates in the WCAD-treated and 5-FU-treated groups were obviously lower than that in the untreated control group (P<0.05 or P<0.01) while the AI was higher than that in the untreated control group (P<0.01). The growth-inhibiting rate, total PCNA-positive rate and total metastasis rate in the WCAD-treated group had no significant differences as compared with those in the 5-FU-treated group, but the AI in the WCAD-treated group was significantly higher than that in the 5-FU-treated group (P<0.05).

Conclusion

WCAD has the inhibiting effects on tumor growth and metastasis of gastric cancer which is orthotopic implanted onto nude mice. This effect may be obtained by proliferation suppression and apoptosis induction in cancer.

Key words: Stomach neoplasms, Compounds (TCD), Neoplasm transplantation, Apoptosis

CLC Number:

R286.91

Hai-lei Zhao, Ai-guang Zhao, Sheng-fu You, Ying Gu, Lai-di Tang, Jin-kun Yang. Growth-inhibiting and anti-metastasis effects of Weichang'an Decoction on orthotopic transplant nude mouse model of human gastric cancer. Journal of Chinese Integrative Medicine, 2005, 3(5): 378-381.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20050512

http://www.jcimjournal.com/EN/Y2005/V3/I5/378

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Group	n	Perigastric lymph nodes	Liver	Lymph nodes in the porta hepatis	Diaphragm	Peritoneum	Total metastasis rate
WCAD-treated	13	0	7.69 (1/13)	0	7.69(1/13)	15.38 (2/13)	30.77(4/13)*
5-FU-treated	14	0	21.43 (3/14)	0	0	14.29 (2/14)	28.57(4/14)*
Untreated control	14	7.14(1/14)	50 (7/14)	28.57 (4/14)	28.57 (4/14)	42.86 (6/14)	71.43 (10/14)

Group	n	Total PCNA- positive rate	Apoptotic index
WCAD-treated	8	39.03±7.37**	9.72±4.51**^△
5-FU-treated	9	43.48±19.77*	5.74±1.75**
Untreated control	8	62.26±13.80	2.45±1.37

Growth-inhibiting and anti-metastasis effects of Weichang'an Decoction on orthotopic transplant nude mouse model of human gastric cancer

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