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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (5): 378-381.doi: 10.3736/jcim20050512

• Original Experimental Research • Previous Articles     Next Articles

Growth-inhibiting and anti-metastasis effects of Weichang'an Decoction on orthotopic transplant nude mouse model of human gastric cancer

Hai-lei Zhao1,Ai-guang Zhao1,Sheng-fu You2,Ying Gu1,Lai-di Tang2,Jin-kun Yang1   

  1. 1. Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    2. Science and Research Laboratory, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2005-04-25 Online:2005-09-20 Published:2005-09-20
  • Contact: Ai-guang Zhao E-mail:aiguang@hotmail.com

Objective

To evaluate the growth-inhibiting and anti-metastasis effects of Weichang'an Decoction (WCAD) on orthotopic transplant nude mouse model of human gastric cancer.

Methods

Forty-one nude mice were implanted with SGC-7901 cells at orthotopic site, whereas 25 were implanted with SGC-7901 cells subcutaneously. Then the nude mice in each transplantation model were divided into the same three groups which were WCAD-treated group with WCAD 0.5 ml/d taken orally, 5-FU-treated group with 5-FU 50 mg/kg intraperitoneally injected weekly and untreated control group with physiological saline 0.5 ml/d taken orally. The growth-inhibiting rates of transplant tumors were detected and the metastatic lesions were examined in the orthotopic transplant mouse model while PCNA-positive rate and apoptotic index (AI) were observed in the subcutaneous transplant mouse model.

Results

The growth-inhibiting rates in the WCAD-treated and 5-FU-treated groups of orthotopic transplant mouse model were 40.82% and 37.92% respectively whereas those of subcutaneous transplant mouse model were 48.70% and 60.10%. The incidence rates of metastasis in perigastric lymph notes, lymph nodes in the porta hepatis, liver, diaphragm and peritoneum in the WCAD-treated and 5-FU-treated groups were lower than those in the untreated control group, and the total metastasis rates in the WCAD-treated, 5-FU-treated and the untreated control groups were 30.77%, 28.57% and 71.43% respectively with significant differences (P<0.05). The total PCNA-positive rates in the WCAD-treated and 5-FU-treated groups were obviously lower than that in the untreated control group (P<0.05 or P<0.01) while the AI was higher than that in the untreated control group (P<0.01). The growth-inhibiting rate, total PCNA-positive rate and total metastasis rate in the WCAD-treated group had no significant differences as compared with those in the 5-FU-treated group, but the AI in the WCAD-treated group was significantly higher than that in the 5-FU-treated group (P<0.05).

Conclusion

WCAD has the inhibiting effects on tumor growth and metastasis of gastric cancer which is orthotopic implanted onto nude mice. This effect may be obtained by proliferation suppression and apoptosis induction in cancer.

Key words: Stomach neoplasms, Compounds (TCD), Neoplasm transplantation, Apoptosis

CLC Number: 

  • R286.91

Tab 1

Tumor metastasis of orthotopic transplant nude mouse model of human gastric cancer in 3 groups (%)"

Group n Perigastric
lymph nodes
Liver Lymph nodes in the
porta hepatis
Diaphragm Peritoneum Total metastasis
rate
WCAD-treated 13 0 7.69 (1/13) 0 7.69(1/13) 15.38 (2/13) 30.77(4/13)*
5-FU-treated 14 0 21.43 (3/14) 0 0 14.29 (2/14) 28.57(4/14)*
Untreated control 14 7.14(1/14) 50 (7/14) 28.57 (4/14) 28.57 (4/14) 42.86 (6/14) 71.43 (10/14)

Tab 2

Expression of PCNA and apoptotic index in subcutaneous transplant nude mouse model of human gastric cancer in 3 groups ($\bar{x}$±s, %)"

Group n Total PCNA-
positive rate
Apoptotic index
WCAD-treated 8 39.03±7.37** 9.72±4.51**
5-FU-treated 9 43.48±19.77* 5.74±1.75**
Untreated control 8 62.26±13.80 2.45±1.37
[1] 李佩文 . 中医药对维护肿瘤患者生活质量的作用[J]. 中华肿瘤杂志, 2002,24(2):203-204
[2] 王坤根 . 中医辨证治疗胃癌93例[J]. 中医杂志, 2004,45(6):441-442
[3] 邱佳信, 贾筠生, 杨金坤 , 等. 健脾法为主治疗晚期胃癌的探讨[J]. 中医杂志, 1992,33(8):23-25
[4] 赵爱光, 杨金坤, 赵海磊 . 健脾中药诱导人胃癌细胞裸小鼠移植瘤细胞凋亡的实验研究[J]. 世界华人消化杂志, 2000,8(7):737-740
doi: 10.3969/j.issn.1009-3079.2000.07.004
[5] 赵爱光, 赵海磊, 杨金坤 , 等. 人胃癌原位移植模型中宿主脾虚证的探讨[J]. 中国中西医结合消化杂志, 2001,9(4):198-200
doi: 10.3969/j.issn.1671-038X.2001.04.002
[6] 王立生, 潘令嘉, 郑跃杰 , 等 .双歧杆菌对裸鼠腹腔巨噬细胞分泌IL-1以及IL-2的影响[J].中国肿瘤生物治疗杂志, 1998, 5(3): 214, 203
[7] Zhao AG, Zhao HL, Jin XJ , et al. Effects of Chinese Jianpi herbs on cell apoptosis and related gene expression in human gastric cancer grafted onto nude mice[J]. World J Gastroentrerol, 2002,8(5):792-796
doi: 10.3748/wjg.v8.i5.792
[8] 赵怡, 张学庸, 史宪杰 , 等. 胃癌组织p16, p53蛋白和增殖细胞核抗原的表达意义[J]. 世界华人消化杂志, 1999,7(3):246-248
doi: 10.3969/j.issn.1009-3079.1999.03.021
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