SUBSCRIBE
Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (5): 378-381.doi: 10.3736/jcim20050512

• Original Experimental Research • Previous Articles     Next Articles

Growth-inhibiting and anti-metastasis effects of Weichang'an Decoction on orthotopic transplant nude mouse model of human gastric cancer

Hai-lei Zhao1,Ai-guang Zhao1,Sheng-fu You2,Ying Gu1,Lai-di Tang2,Jin-kun Yang1   

  1. 1. Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    2. Science and Research Laboratory, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2005-04-25 Online:2005-09-20 Published:2005-09-20
  • Contact: Ai-guang Zhao E-mail:aiguang@hotmail.com

RichHTML

1

PDF (PC)

45

Like

Objective

To evaluate the growth-inhibiting and anti-metastasis effects of Weichang'an Decoction (WCAD) on orthotopic transplant nude mouse model of human gastric cancer.

Methods

Forty-one nude mice were implanted with SGC-7901 cells at orthotopic site, whereas 25 were implanted with SGC-7901 cells subcutaneously. Then the nude mice in each transplantation model were divided into the same three groups which were WCAD-treated group with WCAD 0.5 ml/d taken orally, 5-FU-treated group with 5-FU 50 mg/kg intraperitoneally injected weekly and untreated control group with physiological saline 0.5 ml/d taken orally. The growth-inhibiting rates of transplant tumors were detected and the metastatic lesions were examined in the orthotopic transplant mouse model while PCNA-positive rate and apoptotic index (AI) were observed in the subcutaneous transplant mouse model.

Results

The growth-inhibiting rates in the WCAD-treated and 5-FU-treated groups of orthotopic transplant mouse model were 40.82% and 37.92% respectively whereas those of subcutaneous transplant mouse model were 48.70% and 60.10%. The incidence rates of metastasis in perigastric lymph notes, lymph nodes in the porta hepatis, liver, diaphragm and peritoneum in the WCAD-treated and 5-FU-treated groups were lower than those in the untreated control group, and the total metastasis rates in the WCAD-treated, 5-FU-treated and the untreated control groups were 30.77%, 28.57% and 71.43% respectively with significant differences (P<0.05). The total PCNA-positive rates in the WCAD-treated and 5-FU-treated groups were obviously lower than that in the untreated control group (P<0.05 or P<0.01) while the AI was higher than that in the untreated control group (P<0.01). The growth-inhibiting rate, total PCNA-positive rate and total metastasis rate in the WCAD-treated group had no significant differences as compared with those in the 5-FU-treated group, but the AI in the WCAD-treated group was significantly higher than that in the 5-FU-treated group (P<0.05).

Conclusion

WCAD has the inhibiting effects on tumor growth and metastasis of gastric cancer which is orthotopic implanted onto nude mice. This effect may be obtained by proliferation suppression and apoptosis induction in cancer.

Key words: Stomach neoplasms, Compounds (TCD), Neoplasm transplantation, Apoptosis

CLC Number: 

  • R286.91

Tab 1

Tumor metastasis of orthotopic transplant nude mouse model of human gastric cancer in 3 groups （%）"

Group n Perigastric
lymph nodes		 Liver Lymph nodes in the
porta hepatis		 Diaphragm Peritoneum Total metastasis
rate
WCAD-treated 13 0 7.69 (1/13) 0 7.69(1/13) 15.38 (2/13) 30.77(4/13)*
5-FU-treated 14 0 21.43 (3/14) 0 0 14.29 (2/14) 28.57(4/14)*
Untreated control 14 7.14(1/14) 50 (7/14) 28.57 (4/14) 28.57 (4/14) 42.86 (6/14) 71.43 (10/14)

Tab 2

Expression of PCNA and apoptotic index in subcutaneous transplant nude mouse model of human gastric cancer in 3 groups （$\bar{x}$±s, %）"

Group n Total PCNA-
positive rate		 Apoptotic index
WCAD-treated 8 39.03±7.37** 9.72±4.51**△
5-FU-treated 9 43.48±19.77* 5.74±1.75**
Untreated control 8 62.26±13.80 2.45±1.37
[1] 李佩文 . 中医药对维护肿瘤患者生活质量的作用[J]. 中华肿瘤杂志, 2002,24(2):203-204
[2] 王坤根 . 中医辨证治疗胃癌93例[J]. 中医杂志, 2004,45(6):441-442
[3] 邱佳信, 贾筠生, 杨金坤 , 等. 健脾法为主治疗晚期胃癌的探讨[J]. 中医杂志, 1992,33(8):23-25
[4] 赵爱光, 杨金坤, 赵海磊 . 健脾中药诱导人胃癌细胞裸小鼠移植瘤细胞凋亡的实验研究[J]. 世界华人消化杂志, 2000,8(7):737-740
doi: 10.3969/j.issn.1009-3079.2000.07.004
[5] 赵爱光, 赵海磊, 杨金坤 , 等. 人胃癌原位移植模型中宿主脾虚证的探讨[J]. 中国中西医结合消化杂志, 2001,9(4):198-200
doi: 10.3969/j.issn.1671-038X.2001.04.002
[6] 王立生, 潘令嘉, 郑跃杰 , 等 .双歧杆菌对裸鼠腹腔巨噬细胞分泌IL-1以及IL-2的影响[J].中国肿瘤生物治疗杂志, 1998, 5(3): 214, 203
[7] Zhao AG, Zhao HL, Jin XJ , et al. Effects of Chinese Jianpi herbs on cell apoptosis and related gene expression in human gastric cancer grafted onto nude mice[J]. World J Gastroentrerol, 2002,8(5):792-796
doi: 10.3748/wjg.v8.i5.792
[8] 赵怡, 张学庸, 史宪杰 , 等. 胃癌组织p16, p53蛋白和增殖细胞核抗原的表达意义[J]. 世界华人消化杂志, 1999,7(3):246-248
doi: 10.3969/j.issn.1009-3079.1999.03.021
[1] Maged Mohamed Maher Abou-Hashem, Dina Mohamed Abo-elmatty, Noha Mostafa Mesbah, Ahmed Mohamed Abd EL-Mawgoud. Induction of sub-G0 arrest and apoptosis by seed extract of Moringa peregrina (Forssk.) Fiori in cervical and prostate cancer cell lines. Journal of Integrative Medicine, 2019, 17(6): 410-422.
[2] Supakanya Kumkarnjana, Rutt Suttisri, Ubonthip Nimmannit, Apirada Sucontphunt, Mattaka Khongkow, Thongchai Koobkokkruad, Nontima Vardhanabhuti. Flavonoids kaempferide and 4,2′-dihydroxy-4′,5′,6′-trimethoxychalcone inhibit mitotic clonal expansion and induce apoptosis during the early phase of adipogenesis in 3T3-L1 cells. Journal of Integrative Medicine, 2019, 17(4): 288-295.
[3] Shriniwas S. Basaiyye, Sanjay Kashyap, Kannan Krishnamurthi, Saravanadevi Sivanesan. Induction of apoptosis in leukemic cells by the alkaloid extract of garden cress (Lepidium sativum L.). Journal of Integrative Medicine, 2019, 17(3): 221-228.
[4] Carlos R. Oliveira, Daniel G. Spindola, Daniel M. Garcia, Adolfo Erustes, Alexandre Bechara, Caroline Palmeira-dos-Santos, Soraya S. Smaili, Gustavo J.S. Pereira, André Hinsberger, Ezequiel P. Viriato, Maria Cristina Marcucci, Alexandra C.H.F. Sawaya, Samantha L. Tomaz, Elaine G. Rodrigues, Claudia Bincoletto. Medicinal properties of Angelica archangelica root extract: Cytotoxicity in breast cancer cells and its protective effects against in vivo tumor development. Journal of Integrative Medicine, 2019, 17(2): 132-140.
[5] Bruno José Martins Da Silva, Sandro Wilson Gomes Pereira, Ana Paula Drummond Rodrigues, José Luiz Martins Do Nascimento, Edilene Oliveira Silva. In vitro antileishmanial effects of Physalis angulata root extract on Leishmania infantum. Journal of Integrative Medicine, 2018, 16(6): 404-410.
[6] Eric Wei Chiang Chan, Siu Kuin Wong, Hung Tuck Chan . Casticin from Vitex species: A short review on its anticancer and anti-inflammatory properties. Journal of Integrative Medicine, 2018, 16(3): 147-152.
[7] Xuan Liu, Li-juan Xiu, Jian-peng Jiao, Jing Zhao, Ying Zhao, Ye Lu, Jun Shi, Yong-jin Li, Min Ye, Yu-fang Gu, Xiao-wei Wang, Jing-yu Xu, Ci-an Zhang, Yong-ying Liu, Yun Luo, Xiao-qiang Yue. Traditional Chinese medicine integrated with chemotherapy for stage IV non-surgical gastric cancer: A retrospective clinical analysis. Journal of Integrative Medicine, 2017, 15(6): 469-475.
[8] Jesmin Mondal, Asmita Samadder, Anisur Rahman Khuda-Bukhsh. Psorinum 6× triggers apoptosis signals in human lung cancer cells. Journal of Integrative Medicine, 2016, 14(2): 143-153.
[9] Qing-qing Zang, Lu Zhang, Ning Gao, Cheng Huang. Ophiopogonin D inhibits cell proliferation, causes cell cycle arrest at G2/M, and induces apoptosis in human breast carcinoma MCF-7 cells. Journal of Integrative Medicine, 2016, 14(1): 51-59.
[10] Santu Kumar Saha, Sourav Roy, Anisur Rahman Khuda-Bukhsh. Ultra-highly diluted plant extracts of Hydrastis canadensis and Marsdenia condurango induce epigenetic modifications and alter gene expression profiles in HeLa cells in vitro. Journal of Integrative Medicine, 2015, 13(6): 400-411.
[11] Bing He, Ge Zhang, Ai-ping Lu. Integrative network analysis: Bridging the gap between Western medicine and traditional Chinese medicine. Journal of Integrative Medicine, 2015, 13(3): 133-135.
[12] Samrat Ghosh, Sourav Sikdar, Avinaba Mukherjee, Anisur Rahman Khuda-Bukhsh. Evaluation of chemopreventive potentials of ethanolic extract of Ruta graveolens against A375 skin melanoma cells in vitro and induced skin cancer in mice in vivo. Journal of Integrative Medicine, 2015, 13(1): 34-44.
[13] Samrat Ghosh, Kausik Bishayee, Anisur Rahman Khuda-Bukhsh​. Oleanolic acid isolated from ethanolic extract of Phytolacca decandra induces apoptosis in A375 skin melanoma cells: Drug-DNA interaction and signaling cascade. Journal of Integrative Medicine, 2014, 12(2): 102-114.
[14] Paul Avijit, Das Sreemanti, Das Jayeeta, Samadder Asmita, Bishayee Kausik, Sadhukhan Ratan, Anisur Rahman Khuda-Bukhsh. Diarylheptanoid-myricanone isolated from ethanolic extract of Myrica cerifera shows anticancer effects on HeLa and PC3 cell lines: Signalling pathway and drug-DNA interaction. Journal of Chinese Integrative Medicine, 2013, 11(6): 405-415.
[15] Farha A. Kabeer, Geetha B. Sreedevi, Mangalam S. Nair, Dhanya S. Rajalekshmi, Latha P. Gopalakrishnan, Sujathan Kunjuraman, Remani Prathapan. Antineoplastic effects of deoxyelephantopin, a sesquiterpene lactone from Elephantopus scaber, on lung adenocarcinoma (A549) cells. Journal of Chinese Integrative Medicine, 2013, 11(4): 269-277.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Li Zhou, Hong-xing Zhang, Ling-guang Liu, Wen-jun Wan. Effect of electro-acupuncture at Fenglong (GV 16) on nitric oxide and endothelin in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 89-92
[2] Tao Wang , Feng Qin. Effects of Chinese herbal medicine Xiaoyao Powder on monoamine neurotransmitters in hippocampus of rats with postpartum depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1075-1079
[3] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[4] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[5] Xiang-ying Mao , Qin Bian , Zi-yin Shen. Analysis of the osteogenetic effects exerted on mesenchymal stem cell strain C3H10T1/2 by icariin via MAPK signaling pathway in vitro. Journal of Chinese Integrative Medicine, 2012, 10(11): 1272-1278
[6] Li-fang Wang, Jin-feng Wang, Wei-fang Jin, Hong-fu Wang, Shao-fen Zhang, Jian-jun Gao. Effects of daidzein on steroid receptor coactivator-1 expression in MC3T3-E1 cells and the mechanism. Journal of Chinese Integrative Medicine, 2011, 9(11): 1248-1253
[7] Yi Chen, Wen-jian Wang. Relationships between insulin resistant and hypertension and traditional Chinese medicine intervention. Journal of Chinese Integrative Medicine, 2010, 8(12): 1114-1118
[8] Xin-xiang Wang , Yun-ling Zhang, Qi-fu Huang. Discussion on the main pathogenesis in traditional Chinese medicine and etiology about primary osteoporosis. Journal of Chinese Integrative Medicine, 2010, 8(12): 1119-1123
[9] Kai Qu, Tian-fang Wang, Yan Zhao, Xiao-lin Xue, Xiu-yan Wu, Hua-liang Deng, Lu Yang, Li-liYu , Hong Yue, Qing-guo Wang. Common syndrome factors of traditional Chinese medicine in chronic renal failure based on the questionnaire investigation among experts. Journal of Chinese Integrative Medicine, 2010, 8(12): 1147-1152
[10] Xiao-yan Li , Ze-huai Wen, Wei-xiong Liang , Wei-hua Xu, Su-yun Li, Xue-qing Yu , Hai-feng Wang. Evaluation of consistency of tongue and pulse signs observed by traditional Chinese medicine clinicians. Journal of Chinese Integrative Medicine, 2010, 8(12): 1153-1158
Journal of Integrative Medicine, ISSN: 2095-4964 CN 31-2083/R
Copyright © Journal of Integrative Medicine
Powered by Beijing Magtech Co. Ltd
沪公网安备31010602003416号      沪ICP备18017633号-3