Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (3): 274-277.doi: 10.3736/jcim20080311

• Original Experimental Research • Previous Articles     Next Articles

Effects of arsenic trioxide on apoptosis and proliferation of human lung cancer cells under hypoxia

Jie Shen(), Ge-ping Qu, Qing-yu Xiu, Bing Li   

  1. Department of Respiratory Diseases, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
  • Received:2007-06-12 Online:2008-03-20 Published:2008-03-15
  • Contact: J Shen E-mail:stevejohn@126.com

Objective: To observe the effects of different concentrations of arsenic trioxide (As2O3) on apoptosis and proliferation of human lung cancer cell line A549 in vitro under hypoxia and normoxia.Methods: A549 cells were treated with 0, 1, 2, 4 μmol/L As2O3 for 12, 24 and 48 h under hypoxia (5% O2) and normoxia (21% O2). The proliferative inhibition rate of A549 cells was measured with methyl thiazolyl tetrazolium assay, and the apoptotic rate of A549 cells was detected by Annexin Ⅴ/propidium iodide (PI) double staining.Results: Under normoxia and hypoxia, 1, 2, 4 μmol/L As2O3 could significantly inhibit the proliferation of A549 cells and induce the apoptosis of A549 cells. The results depended on the drug concentration and action time. And the hypoxia couldn't influence the effects of As2O3.Conclusion: As2O3 can inhibit the proliferation and induce the apoptosis of A549 cells under hypoxia and normoxia.

Key words: arsenic compounds, hypoxia, apoptosis, cell proliferation, lung cancer

CLC Number: 

  • R285.5

Figure 1

Proliferative inhibition rate of A549 cells treated with different dose arsenic trioxide for different action time under normoxia and hypoxia"

Figure 2

Early apoptotic rate of A549 cells treated with different dose arsenic trioxide for different action time under normoxia and hypoxia"

Figure 3

Late apoptotic rate of A549 cells treated with different dose arsenic trioxide for different action time under normoxia and hypoxia"

[1] Wang ZY, Chen Z.Differentiation and apoptosis induction therapy in acute promyelocytic leukemia[J].Lancet Oncol, 2000, 1(): 101-106
[2] McCafferty-Grad J, Bahlis NJ, Krett N , et al. Arsenic trioxide uses caspase-dependent and caspase-independent death pathways in myeloma cells[J]. Mol Cancer Ther, 2003,2(11):1155-1164
doi: 10.1002/mpo.10362 pmid: 14617789
[3] Monzen H, Griffin RJ, Williams BW , et al. Study of arsenic trioxide-induced vascular shutdown and enhancement with radiation in solid tumor[J]. Radiat Med, 2002,22(4):205-211
[4] Bangoura G, Yang LY, Huang GW , et al. Expression of HIF-2alpha/EPAS1 in hepatocellular carcinoma[J]. World J Gastroenterol, 2004,10(4):525-530
doi: 10.3748/wjg.v10.i4.525
[5] Bos R, van der Groep P, Greijer AE , et al. Levels of hypoxia-inducible factor-1alpha independently predict prognosis in patients with lymph node negative breast carcinoma[J]. Cancer, 2003,97(6):1573-1581
doi: 10.1002/(ISSN)1097-0142
[6] Nakayama K, Kanzaki A, Hata K , et al. Hypoxia-inducible factor 1 alpha(HIF-1 alpha) gene expression in human ovarian carcinoma[J]. Cancer Lett, 2002,176(2):215-223
doi: 10.1016/S0304-3835(01)00762-5
[7] Karlsson J, Edsj A, Phlman S , et al. Multidrug-resistant neuroblastoma cells are responsive to arsenic trioxide at both normoxia and hypoxia[J]. Mol Cancer Ther, 2005,4(7):1128-1135
doi: 10.1158/1535-7163.MCT-05-0047
[8] Lew YS, Brown SL, Griffin RJ , et al. Arsenic trioxide causes selective necrosis in solid murine tumors by vascular shutdown[J]. Cancer Res, 1999,59(24):6033-6037
pmid: 10626785
[9] Griffin RJ, Monzen H, Williams BW , et al. Arsenic trioxide induces selective tumor vascular damage via oxidative stress and increases thermosensitivity of tumors[J]. Int J Hyperthermia, 2003,19(6):575-589
doi: 10.1080/0265673031000124316
[10] Birner P, Gatterbauer B, Oberhuber G , et al. Expression of hypoxia-inducible factor-1 alpha in oligod endrogliomas: its impact on prognosis and on neoangiogenesis[J]. Cancer, 2001,92(1):165-171
doi: 10.1002/cncr.10462
[11] Samali A, Cotter TG . Heat shock proteins increase resistance to apoptosis[J]. Exp Cell Res, 1996,223(1):163-170
doi: 10.1006/excr.1996.0070 pmid: 8635489
[12] Zhivotovsky B, Joseph B, Orrenius S . Tumor radiosensitivity and apoptosis[J]. Exp Cell Res, 1999,48(1):10-17
[13] Yi J, Yang J, He R , et al. Emodin enhances arsenic trioxide-induced apoptosis via generation of reactive oxygen species and inhibition of survival signaling[J]. Cancer Res, 2004,64(1):108-116
doi: 10.1158/0008-5472.CAN-2820-2
[14] Park MJ, Park IC, Bae IJ , et al. Tetraarsenic oxide, a novel orally administrable angiogenesis inhibitor[J]. Int J Oncol, 2003,22(6):1271-1276
doi: 10.3892/ijo.22.6.1271 pmid: 12738993
[15] Ling YH, Jiang JD, Holland JF , et al. Arsenic trioxide produces polymerization of microtubules and mitotic arrest before apoptosis in human tumor cell lines[J]. Mol Pharmacol, 2002,62(3):529-538
doi: 10.1124/mol.62.3.529
[1] Maged Mohamed Maher Abou-Hashem, Dina Mohamed Abo-elmatty, Noha Mostafa Mesbah, Ahmed Mohamed Abd EL-Mawgoud. Induction of sub-G0 arrest and apoptosis by seed extract of Moringa peregrina (Forssk.) Fiori in cervical and prostate cancer cell lines. Journal of Integrative Medicine, 2019, 17(6): 410-422.
[2] Supakanya Kumkarnjana, Rutt Suttisri, Ubonthip Nimmannit, Apirada Sucontphunt, Mattaka Khongkow, Thongchai Koobkokkruad, Nontima Vardhanabhuti. Flavonoids kaempferide and 4,2′-dihydroxy-4′,5′,6′-trimethoxychalcone inhibit mitotic clonal expansion and induce apoptosis during the early phase of adipogenesis in 3T3-L1 cells. Journal of Integrative Medicine, 2019, 17(4): 288-295.
[3] Shriniwas S. Basaiyye, Sanjay Kashyap, Kannan Krishnamurthi, Saravanadevi Sivanesan. Induction of apoptosis in leukemic cells by the alkaloid extract of garden cress (Lepidium sativum L.). Journal of Integrative Medicine, 2019, 17(3): 221-228.
[4] Carlos R. Oliveira, Daniel G. Spindola, Daniel M. Garcia, Adolfo Erustes, Alexandre Bechara, Caroline Palmeira-dos-Santos, Soraya S. Smaili, Gustavo J.S. Pereira, André Hinsberger, Ezequiel P. Viriato, Maria Cristina Marcucci, Alexandra C.H.F. Sawaya, Samantha L. Tomaz, Elaine G. Rodrigues, Claudia Bincoletto. Medicinal properties of Angelica archangelica root extract: Cytotoxicity in breast cancer cells and its protective effects against in vivo tumor development. Journal of Integrative Medicine, 2019, 17(2): 132-140.
[5] Syed Nasir Abbas Bukhari, Fahad Hussain, Hnin Ei Thu, Zahid Hussain. Synergistic effects of combined therapy of curcumin and Fructus Ligustri Lucidi for treatment of osteoporosis: cellular and molecular evidence of enhanced bone formation. Journal of Integrative Medicine, 2019, 17(1): 38-45.
[6] Goulnar Kasymjanova, Anh-Thi Tran, Victor Cohen, Carmela Pepe, Lama Sakr, David Small, Jason Scott Agulnik, Robert Thomas Jagoe. The use of a standardized Chinese herbal formula in patients with advanced lung cancer: A feasibility study. Journal of Integrative Medicine, 2018, 16(6): 390-395.
[7] Bruno José Martins Da Silva, Sandro Wilson Gomes Pereira, Ana Paula Drummond Rodrigues, José Luiz Martins Do Nascimento, Edilene Oliveira Silva. In vitro antileishmanial effects of Physalis angulata root extract on Leishmania infantum. Journal of Integrative Medicine, 2018, 16(6): 404-410.
[8] Eric Wei Chiang Chan, Siu Kuin Wong, Hung Tuck Chan . Casticin from Vitex species: A short review on its anticancer and anti-inflammatory properties. Journal of Integrative Medicine, 2018, 16(3): 147-152.
[9] Shui-jie Shen, Yong-hong Zhang, Xiao-xia Gu, Shui-ju Jiang, Ling-jun Xu. Yangfei Kongliu Formula, a compound Chinese herbal medicine, combined with cisplatin, inhibits growth of lung cancer cells through transforming growth factor-β1 signaling pathway. Journal of Integrative Medicine, 2017, 15(3): 242-251.
[10] Jesmin Mondal, Asmita Samadder, Anisur Rahman Khuda-Bukhsh. Psorinum 6× triggers apoptosis signals in human lung cancer cells. Journal of Integrative Medicine, 2016, 14(2): 143-153.
[11] Qing-qing Zang, Lu Zhang, Ning Gao, Cheng Huang. Ophiopogonin D inhibits cell proliferation, causes cell cycle arrest at G2/M, and induces apoptosis in human breast carcinoma MCF-7 cells. Journal of Integrative Medicine, 2016, 14(1): 51-59.
[12] Santu Kumar Saha, Sourav Roy, Anisur Rahman Khuda-Bukhsh. Ultra-highly diluted plant extracts of Hydrastis canadensis and Marsdenia condurango induce epigenetic modifications and alter gene expression profiles in HeLa cells in vitro. Journal of Integrative Medicine, 2015, 13(6): 400-411.
[13] Samrat Ghosh, Sourav Sikdar, Avinaba Mukherjee, Anisur Rahman Khuda-Bukhsh. Evaluation of chemopreventive potentials of ethanolic extract of Ruta graveolens against A375 skin melanoma cells in vitro and induced skin cancer in mice in vivo. Journal of Integrative Medicine, 2015, 13(1): 34-44.
[14] Zhong-liang Liu, Wei-rong Zhu, Wen-chao Zhou, Hai-feng Ying, Lan Zheng, Yuan-biao Guo, Jing-xian Chen, Xiao-heng Shen. Traditional Chinese medicinal herbs combined with epidermal growth factor receptor tyrosine kinase inhibitor for advanced non-small cell lung cancer: A systematic review and meta-analysis. Journal of Integrative Medicine, 2014, 12(4): 346-358.
[15] Samrat Ghosh, Kausik Bishayee, Anisur Rahman Khuda-Bukhsh​. Oleanolic acid isolated from ethanolic extract of Phytolacca decandra induces apoptosis in A375 skin melanoma cells: Drug-DNA interaction and signaling cascade. Journal of Integrative Medicine, 2014, 12(2): 102-114.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Zhao-guo Li , Rui-qiang Liu. Comparative study on WHO Western Pacific Region and World Federation of Chinese Medicine Societies international standard terminologies on traditional medicine: Diseases in Internal Medicine (Part 7). Journal of Chinese Integrative Medicine, 2012, 10(11): 1310-1315
[2] Ji Chen, Qin Pan, Li-shuang Ye, Jia-ling Huang. English translation of cultural aspects of the titles of traditional Chinese medicine classics based on the skopos theory. Journal of Chinese Integrative Medicine, 2012, 10(11): 1316-1320
[3] Li-fang Wang, Jin-feng Wang, Wei-fang Jin, Hong-fu Wang, Shao-fen Zhang, Jian-jun Gao. Effects of daidzein on steroid receptor coactivator-1 expression in MC3T3-E1 cells and the mechanism. Journal of Chinese Integrative Medicine, 2011, 9(11): 1248-1253
[4] Zhu-yi Zhang, Shi-qing Yu, Li-yun Miao, Xiao-ying Huang, Xiao-ping Zhang, Yu-ping Zhu, Xiao-hong Xia, Dan-qi Li. Artesunate combined with vinorelbine plus cisplatin in treatment of advanced non-small cell lung cancer: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(2): 134-138
[5] Sheng-lan Yang, Dao-ben Li. Clinical application of dredging intestines. Journal of Chinese Integrative Medicine, 2005, 3(1): 59-60
[6] Vamsi Reddy, Arvind Sridhar, Roberto F. Machado, Jiwang Chen. High sodium causes hypertension: Evidence from clinical trials and animal experiments. Journal of Integrative Medicine, 2015, 13(1): 1-8
[7] Ya Yuwen, Yu-qi Liu, Yan-ping Wang, Jin-gang Dai, Da-sheng Liu, Yue-xi Wang, Xue-jie Han. The add-on effect of a Chinese herbal formula for patients with resistant hypertension: Study protocol for a pilot cohort study. Journal of Integrative Medicine, 2015, 13(2): 122-128
[8] Qian-jun Chen, Yi-an Wang, Hong-lin Situ, De-min Lu, Li-ping Ren, Xi-wen Lai, Peng-xi Liu, Yi Lin. Effects of Runing RecipeⅡ on expressions of p53 and ras oncogene proteins and cell cycle of the transplanted Ca761 breast cancer in mice. Journal of Chinese Integrative Medicine, 2005, 3(3): 225-228
[9] Jian-xiong Zhao, Wei-dong Cheng, Rui-feng Xu, Yuan-qing Li. Effects of Fuzheng Yiliu Granule-medicated serum on apoptosis of liver cancer cells from mice and its mechanism. Journal of Chinese Integrative Medicine, 2005, 3(4): 278-281
[10] Wei Deng, Fang Shu, Jing Chen. Treatment of diabetic nephropathy with integrated traditional Chinese and Western medicine: A report of 30 cases. Journal of Chinese Integrative Medicine, 2005, 3(4): 314-315