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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (3): 274-277.doi: 10.3736/jcim20080311

• Original Experimental Research • Previous Articles     Next Articles

Effects of arsenic trioxide on apoptosis and proliferation of human lung cancer cells under hypoxia

Jie Shen(), Ge-ping Qu, Qing-yu Xiu, Bing Li   

  1. Department of Respiratory Diseases, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
  • Received:2007-06-12 Online:2008-03-20 Published:2008-03-15
  • Contact: J Shen

Objective: To observe the effects of different concentrations of arsenic trioxide (As2O3) on apoptosis and proliferation of human lung cancer cell line A549 in vitro under hypoxia and normoxia.Methods: A549 cells were treated with 0, 1, 2, 4 μmol/L As2O3 for 12, 24 and 48 h under hypoxia (5% O2) and normoxia (21% O2). The proliferative inhibition rate of A549 cells was measured with methyl thiazolyl tetrazolium assay, and the apoptotic rate of A549 cells was detected by Annexin Ⅴ/propidium iodide (PI) double staining.Results: Under normoxia and hypoxia, 1, 2, 4 μmol/L As2O3 could significantly inhibit the proliferation of A549 cells and induce the apoptosis of A549 cells. The results depended on the drug concentration and action time. And the hypoxia couldn't influence the effects of As2O3.Conclusion: As2O3 can inhibit the proliferation and induce the apoptosis of A549 cells under hypoxia and normoxia.

Key words: arsenic compounds, hypoxia, apoptosis, cell proliferation, lung cancer

CLC Number: 

  • R285.5

Figure 1

Proliferative inhibition rate of A549 cells treated with different dose arsenic trioxide for different action time under normoxia and hypoxia"

Figure 2

Early apoptotic rate of A549 cells treated with different dose arsenic trioxide for different action time under normoxia and hypoxia"

Figure 3

Late apoptotic rate of A549 cells treated with different dose arsenic trioxide for different action time under normoxia and hypoxia"

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