低氧环境下三氧化二砷对人肺癌细胞增殖和凋亡的作用

doi:10.3736/jcim20080311

Abstract

Objective: To observe the effects of different concentrations of arsenic trioxide (As₂O₃) on apoptosis and proliferation of human lung cancer cell line A549 in vitro under hypoxia and normoxia.Methods: A549 cells were treated with 0, 1, 2, 4 μmol/L As₂O₃ for 12, 24 and 48 h under hypoxia (5% O₂) and normoxia (21% O₂). The proliferative inhibition rate of A549 cells was measured with methyl thiazolyl tetrazolium assay, and the apoptotic rate of A549 cells was detected by Annexin Ⅴ/propidium iodide (PI) double staining.Results: Under normoxia and hypoxia, 1, 2, 4 μmol/L As₂O₃could significantly inhibit the proliferation of A549 cells and induce the apoptosis of A549 cells. The results depended on the drug concentration and action time. And the hypoxia couldn't influence the effects of As₂O₃.Conclusion: As₂O₃can inhibit the proliferation and induce the apoptosis of A549 cells under hypoxia and normoxia.

Key words: arsenic compounds, hypoxia, apoptosis, cell proliferation, lung cancer

CLC Number:

R285.5

Jie Shen, Ge-ping Qu, Qing-yu Xiu, Bing Li. Effects of arsenic trioxide on apoptosis and proliferation of human lung cancer cells under hypoxia. Journal of Chinese Integrative Medicine, 2008, 6(3): 274-277.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20080311

http://www.jcimjournal.com/EN/Y2008/V6/I3/274

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References 15

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Effects of arsenic trioxide on apoptosis and proliferation of human lung cancer cells under hypoxia

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