Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (2): 163-167.doi: 10.3736/jcim20090213

• Original Experimental Research • Previous Articles     Next Articles

Preventive and therapeutic effects of Yiqi Huayu Recipe on degeneration of articular cartilage in rats with osteoarthritis

 Wei Houa,Qian-qian Lianga, Jian-xin Heb, Qin Biana, Qi Shia, Yong-jun Wanga   

  1. a Institute of Spine, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    b Department of Orthopaedics, Wujin Hospital of Traditional Chinese Medicine, Changzhou, Jiangsu Province 213161, China
  • Received:2008-07-13 Accepted:2008-09-12 Online:2009-02-20 Published:2009-02-15

Objective

To study the efficacy and possible mechanism of Yiqi Huayu Recipe (YQHYR), a compound traditional Chinese herbal medicine, in preventing and treating degeneration of the articular cartilage in rats with osteoarthritis (OA).
Methods

A total of 90 one-month-old SD rats were randomly divided into normal control group, untreated group and YQHYR group, with 30 rats in each group. The osteoarthritis was induced by shoulder disarticulation plus upright posture in rats. The rats in YQHYR group were treated with YQHYR at 5-, 7- and 9-month old (4, 6 and 8 months after the surgery) for one month. Ten rats in each subgroup were sacrificed at 6-, 8- and 10-month old (5, 7 and 9 months after the surgery) respectively and the knee joint samples were harvested for detection. Safranin-O/fast green staining was performed to examine the morphology of articular cartilage. The expressions of type Ⅱ collagen (Col2A1), aggrecan-1 (Agc1), matrix metalloproteinase-13 (MMP-13) and tissue inhibitor of metalloproteinase-1 (TIMP-1) mRNAs were evaluated by real-time fluorescent quantitation polymerase chain reaction.
Results

Histological analysis showed that the structure of articular cartilage was seriously destroyed in rats in the untreated group. On the contrary, the degenerative changes of the articular cartilage in the YQHYR group were dropped off. Real-time fluorescent quantitation polymerase chain reaction showed that expressions of Agc1, TIMP-1 and Col2A1 mRNAs were up-regulated in 6- and 10-month-old rats in the YQHYR group as compared with those in the untreated group (P<0.01, P<0.05), but there was no significant difference in expression of MMP-13 mRNA between the YQHYR group and the untreated group. The expression of Agc1 mRNA was up-regulated and the expression of MMP-13 mRNA was down-regulated in the 8-month-old rats in YQHYR group as compared with those in the untreated group (P<0.01).
Conclusion

YQHYR can promote the synthesizing of aggrecan and type Ⅱ collagen in chondrocytes and delay articular cartilage degradation in OA rats.

Key words: Yiqi Huayu Recipe, Osteoarthritis, knee, Articular cartilage, Rats

Table 1

Sequence of primers for real time fluorescent quantitation PCR"

Gene Primer Product size (bp)
Col2A1 Forward: 5'-TCC TAA GGG TGC CAA TGG TGA-3'
Reverse: 5'-AGG ACC AAC TTT GCC TTG AGG AC-3'
112
Agc1 Forward: 5'-TCC GCT GGT CTG ATG GAC AC-3'
Reverse: 5'-CCA GAT CAT CAC TAC GCA GTC CTC-3'
101
MMP13 Forward: 5'-CCC TGG AGC CCT GAT GTT T-3'
Reverse: 5'-CTC TGG TGT TTT GGG GTG CT-3'
142
TIMP-1 Forward: 5'-ACA GGT TTC CGG TTC GCC TAC-3'
Reverse: 5'-CTG CAG GCA GTG ATG TGC AA-3'
134
β-actin Forward: 5'-GGA GAT TAC TGC CCT GGC TCC TA-3'
Reverse: 5'-GAC TCA TCG TAC TCC TGC TTG CTG-3'
150

Figure 1

Histological changes of knee joints in 6-, 8- and 10-month-old rats in different groups observed by light microscope (SF staining, ×100)"

Table 2

Expressions of Col2A1, Agc1, MMP-13 and TIMP-1 mRNAs in articular cartilage of knee joints in 6-, 8- and 10-month-old rats(x±s)"

Group n Col2A1 mRNA Agc1 mRNA TIMP-1 mRNA MMP-13 mRNA
6 months
Normal control 6 1.000±0.082 1.000±0.118 1.000±0.026 1.000±0.143
Untreated 6 0.682±0.051** 0.567±0.069* 0.353±0.014** 1.025±0.114
YQHYR 6 0.935±0.063△△ 1.736±0.175*△△ 0.420±0.016** 0.799±0.129
8 months
Normal control 6 0.725±0.051 1.373±0.178 0.673±0.026 3.205±0.432
Untreated 6 0.414±0.072** 0.584±0.025** 0.251±0.023** 6.065±0.511*
YQHYR 6 0.171±0.012 0.862±0.096*△△ 0.266±0.050** 2.140±0.216△△
10 months
Normal control 6 0.266±0.010 1.750±0.213 0.666±0.017 3.945±0.094
Untreated 6 0.139±0.024** 0.474±0.041** 0.154±0.012** 6.026±0.606**
YQHYR 6 0.243±0.024△△ 0.922±0.105**△△ 0.614±0.037△△ 4.983±0.458
[1] Tian ZQ, Wu GB . Recent development in the clinical study of Chinese medicine for knee osteoarthritis[J]. Hunan Zhong Yi Za Zhi, 2008,24(2):6-108
田志清, 吴官保 . 中医药治疗膝骨性关节炎的临床研究进展[J]. 湖南中医杂志, 2008,24(2):6-108
[2] Jiang HP, Wang DP . Animal model of osteoarthritis[J]. Zhongguo Xian Dai Yi Xue Za Zhi, 2004,14(6):153-154, 156
江捍平, 王大平 . 骨关节炎动物模型[J]. 中国现代医学杂志, 2004,14(6):153-154, 156
[3] Miosge N, Hartmann M, Maelicke C, Herken R . Expression of collagen type Ⅰ and type Ⅱ in consecutive stages of human osteoarthritis[J]. Histochem Cell Biol, 2004,122(3):229-236
doi: 10.1007/s00418-004-0697-6
[4] Kiani C, Chen L, Wu YJ, Yee AJ, Yang BB . Structure and function of aggrecan[J]. Cell Res, 2002,12(1):19-32
doi: 10.1038/sj.cr.7290106
[5] Knauper V, López-Otin C, Smith B, Knight G, Murphy G . Biochemical characterization of human collagenase-3[J]. J Biol Chem, 1996,271(3):1544-1550
doi: 10.1074/jbc.271.3.1544
[6] Brew K, Dinakarpandian D, Nagase H . Tissue inhibitors of metalloproteinases: evolution, structure and function[J]. Biochim Biophys Acta, 2000,1477(1-2):267-283
doi: 10.1016/S0167-4838(99)00279-4
[7] Martel-Pelletier J, McCollum R, Fujimoto N, Obata K, Cloutier JM, Pelletier JP .Excess of metallo-proteases over tissue inhibitor of metalloprotease may contribute to cartilage degradation in osteoarthritis and rheumatoid arthritis[J]. Lab Invest, 1994,70(6):807-815
[8] Chen Y, Yu WH . Pharmacological action and clinical application of Radix Astragali and its preparations[J]. Zhongguo Yao Ye, 2006,15(3):68-69
陈雅, 余汶华 . 黄芪及其制剂的药理作用和临床应用进展[J]. 中国药业, 2006,15(3):68-69
[9] Wang WR, Liu HZ, Wei XC, Zhang JY, Yan JP . Experimental study of therapeutic and preventive effects of ligustrazine on OA cartilage degeneration of the knee[J]. Zhongguo Gu Shang, 2004,17(2):80-82
doi: doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz
王文瑞, 刘宏泽, 卫小春, 张剑宇, 闫俊萍 . 川芎嗪防治膝关节软骨退变的实验研究[J]. 中国骨伤, 2004,17(2):80-82
doi: doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz
[10] Wang SL, Wang M, Wu SH . The effect of intra-articular ligustrazine injection on degenerative cartilage of the rabbit's knee[J]. Xi'an Jiao Tong Da Xue Xue Bao Yi Xue Ban, 2006,27(1):62-65
王锁良, 王民, 吴施慧 . 关节内注射川芎嗪对兔膝关节软骨退变的影响[J]. 西安交通大学学报(医学版)., 2006,27(1):62-65
[1] Bing-rong Li, Shi-yun Shao, Long Yuan, Ru Jia, Jian Sun, Qing Ji, Hua Sui, Li-hong Zhou, Yi Zhang, Hui Liu, Qi Li, Yan Wang, Bi-meng Zhang. Effects of mild moxibustion on intestinal microbiome and NLRP3 inflammasome in rats with 5-fluorouracil-induced intestinal mucositis. Journal of Integrative Medicine, 2021, 19(2): 144-157.
[2] Sitthichai Iamsaard, Supatcharee Arun, Jaturon Burawat, Supataechasit Yannasithinon, Saranya Tongpan, Sudtida Bunsueb, Natthapol Lapyuneyong, Pannawat Choowong-in, Nareelak Tangsrisakda, Chadaporn Chaimontri, Wannisa Sukhorum. Evaluation of antioxidant capacity and reproductive toxicity of aqueous extract of Thai Mucuna pruriens seeds. Journal of Integrative Medicine, 2020, 18(3): 265-273.
[3] Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[4] Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[5] Kylie Connolly, Douglas Jackson, Candice Pullen, Andrew Fenning. Alpha-adrenoceptor antagonism by Crassostrea gigas oyster extract inhibits noradrenaline-induced vascular contraction in Wistar rats. Journal of Integrative Medicine, 2015, 13(3): 194-200.
[6] Udhaya Lavinya Baskaran, Sherry Joseph Martin, Rasool Mahaboobkhan, Sabina Evan Prince. Protective role of Triphala, an Indian traditional herbal formulation, against the nephrotoxic effects of bromobenzene in Wistar albino rats. Journal of Integrative Medicine, 2015, 13(2): 115-121.
[7] Hussein O. B. Oloyede, Matthew C. Adaja, Taofeek O. Ajiboye, Musa O. Salawu. Anti-ulcerogenic activity of aqueous extract of Carica papaya seed on indomethacin-induced peptic ulcer in male albino rats. Journal of Integrative Medicine, 2015, 13(2): 105-114.
[8] Anirudha A. Lande, Shirishkumar D. Ambavade, Uma S. Swami, Prafulla P. Adkara Prashant D. Ambavade, Arun B. Waghamare. Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase. Journal of Integrative Medicine, 2015, 13(1): 25-33.
[9] Elizabeth Abidemi Balogun, Sylvia Orume Malomo, Joseph Oluwatope Adebayo, Ahmed Adebayo Ishola, Ayodele Olufemi Soladoye, Lawrence Aderemi Olatunji, Olatunji Matthew Kolawole, Stephen Olubunmi Oguntoye, Abiola Samuel Babatunde, Oluwole Busayo Akinola . In vivo antimalarial activity and toxicological effects of methanolic extract of Cocos nucifera (Dwarf red variety) husk fibre. Journal of Integrative Medicine, 2014, 12(6): 504-511.
[10] Urmila Aswar, Mayuri Gurav, Ganesh More, Khaled Rashed, Manoj Aswar. Effect of aqueous extract of Solanum xanthocarpum Schrad. & Wendl. on postmenopausal syndrome in ovariectomized rats. Journal of Integrative Medicine, 2014, 12(5): 439-446.
[11] Xin-fang Zhang, Ji Zhu, Wen-ye Geng, Shu-jun Zhao, Chuan-wei Jiang, Sheng-rong Cai, Miao Cheng, Chuan-yun Zhou, Zi-bing Liu. Electroacupuncture at Feishu (BL13) and Zusanli (ST36) down-regulates the expression of orexins and their receptors in rats with chronic obstructive pulmonary disease. Journal of Integrative Medicine, 2014, 12(5): 417-424.
[12] Benjamin Perry, Junzeng Zhang, Tarek Saleh, Yanwen Wang. Liuwei Dihuang, a traditional Chinese herbal formula, suppresses chronic inflammation and oxidative stress in obese rats. Journal of Integrative Medicine, 2014, 12(5): 447-454.
[13] Upendarrao Golla, Praveen Kumar Gajam, Solomon Sunder Bhimathati. Evaluation of diuretic and laxative activity of hydro-alcoholic extract of Desmostachya bipinnata (L.) Stapf in rats. Journal of Integrative Medicine, 2014, 12(4): 372-378.
[14] Qian Zhang, Xin-hua Xiao, Ming Li, Wen-hui Li, Miao Yu, Hua-bing Zhang, Fan Ping, Zhi-xin Wang, Jia Zheng ​. Chromium-containing traditional Chinese medicine, Tianmai Xiaoke Tablet improves blood glucose through activating insulin-signaling pathway and inhibiting PTP1B and PCK2 in diabetic rats. Journal of Integrative Medicine, 2014, 12(3): 162-170.
[15] Amir Rashidian​, Saeed Mehrzadi, Ali Reza Ghannadi, Parvin Mahzooni, Samira Sadr, Mohsen Minaiyan​. Protective effect of ginger volatile oil against acetic acid-induced colitis in rats: A light microscopic evaluation. Journal of Integrative Medicine, 2014, 12(2): 115-120.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[2] Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[3] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[4] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[5] Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[6] Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[7] Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[8] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[9] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[10] Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239