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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (8): 753-757.doi: 10.3736/jcim20090809

• Original Experimental Research • Previous Articles     Next Articles

Protective effects of astilbin on renal ischemia-reperfusion injury in rats

Shao-hua Songa,Xiao-yun Shenb,Fang Liua,Yi Tangb,Zhen-meng Wangc,Zhi-ren Fua   

  1. a Organ Transplantation Center, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
    b Institute of Immunology,Second Military Medical University,Shanghai 200433,China
    c Department of Anesthesiology,Eastern Hepatobiliary Surgery Hospital,Second Military Medical University,Shanghai 200433, China
  • Received:2009-04-20 Accepted:2009-06-15 Online:2009-08-20 Published:2009-08-15
  • Contact: Zhi-ren Fu


To investigate the protective effects of astilbin on renal ischemia-reperfusion (IR) injury in rats. 


Twenty-four male SD rats, two months old, were randomly allocated into three groups: sham-operated group (n=8), untreated group (n=8) and astilbin group (n=8). Rats in the untreated group and the astilbin group underwent temporary renal artery occlusion to induce IR injury. The rats in the astilbin group were intraperitoneally injected with 12 mg/mL astilbin at a dose of 30 mg/kg from 3 day before IR injury until to be sacrificed once per day, and rats in the untreated group were injected with equal volume of normal saline at the same time. After 6-hour reperfusion, blood urea nitrogen (BUN) and serum creatinine (SCr) and histological changes of the renal tissues were detected to evaluate renal injury. Expressions of monocyte chemoattractant protein-1 (MCP-1) mRNA and protein in the renal tissues and the serum contents of interleukin-6 (IL-6) and IL-1β were also measured with semi-quantitative reverse transcription-polymerase chain reaction, Western blotting or enzyme-linked immunosorbent assay.

Compared with the untreated group, BUN and SCr levels were significantly decreased in the astilbin group after 6-hour reperfusion (P<0.01), and similar results were also found in histological examination. The expressions of MCP-1 mRNA and protein in renal tissues in the astilbin group were lower than those in the untreated group. The serum contents of IL-6 and IL-1β were decreased in the astilbin group as compared with the untreated group (P<0.01). 


Astilbin can ameliorate kidney IR injury in rats by inhibiting the production of chemokine MCP-1 and cytokines IL-6 and IL-1β.

Key words: Astilbin, Reperfusion injury, Kidney, Rats

Table 1

Contents of BUN and SCr in different groups after 6-hour reperfusion (?x±s)"

Group n BUN (mmol/L) SCr (μmol/L)
Sham-operated 8 7.0±0.4 69.3±5.8
Untreated 8 21.3±2.0** 215.4±18.7**
Astilbin 8 13.0±1.3△△ 122.3±10.0△△

Figure 1

Histological changes of renal tissues in different groups after 6-hour reperfusion observed by HE staining (Light microscopy, ×200)"

Table 2

Histological score of renal tubular injury and expression of MCP-1 mRNA in renal tissues in different groups after 6-hour reperfusion (?x±s)"

Group n Score of renal tubule injury Relative MCP-1 mRNA level
Sham-operated 8 16±6 0.26±0.06
Untreated 8 428±58** 1.69±0.15**
Astilbin 8 226±36△△ 0.38±0.05△△

Figure 2

Expression of MCP-1 protein in renal tissues in different groups after 6-hour reperfusion"

Table 3

Serum contents of IL-6 and IL-1β in different groups after 6-hour reperfusion ($\bar{x}$±s, ng/L)"

Group n IL-6 IL-1β
Sham-operated 8 10.6±1.9 27.7±6.2
Untreated 8 144.5±16.2** 291.7±22.7**
Astilbin 8 45.7±7.6△△ 161.7±17.8△△
[1] Brady HR, Singer GG . Acute renal failure[J]. Lancet, 1995,346(8989):1533-1540
doi: 10.1016/S0140-6736(95)92057-9
[2] Thadhani R, Pascual M, Bonventre JV . Acute renal failure[J]. N Engl J Med, 1996,334(22):1448-1560
doi: 10.1056/NEJM199605303342207
[3] Gao SH, Li P, Chen T, Pan TC, Zhao JP . Effects of astilbin on the expression of IL-2, γ-IFN, IL-4, IL-10, IL-12 and IL-18 in mice heart transplantation rejection[J]. Zhonghua Xiao Er Wai Ke Za Zhi, 2006,27(2):87-89
高思海, 李平, 陈涛, 潘铁成, 赵金平 . 落新妇甙对心脏移植排斥反应中细胞因子表达的影响[J]. 中华小儿外科杂志, 2006,27(2):87-89
[4] Basile DP, Donohoe D, Cao X, Van Why SK . Resistance to ischemic acute renal failure in the Brown Norway rat: a new model to study cytoprotection[J]. Kidney Int, 2004,65(6):2201-2211
doi: 10.1111/j.1523-1755.2004.00637.x
[5] Paller MS, Hoidal JR, Ferris TF . Oxygen free radicals in ischemic acute renal failure in the rat[J]. J Clin Invest, 1984,74(4):1156-1164
doi: 10.1172/JCI111524
[6] Kieran NE, Rabb H . Immune responses in kidney pre-servation and reperfusion injury[J]. J Investig Med, 2004,52(5):310-314
doi: 10.2310/6650.2004.00026 pmid: 15551653
[7] Bonventre JV, Zuk A . Ischemic acute renal failure: an inflammatory disease?[J]. Kidney Int, 2004,66(2):480-485
doi: 10.1111/j.1523-1755.2004.761_2.x
[8] Molitoris BA, Sutton TA . Endothelial injury and dysfunction: role in the extension phase of acute renal failure[J]. Kidney Int, 2004,66(2):496-499
doi: 10.1111/j.1523-1755.2004.761_5.x pmid: 15253696
[9] McCourtie AS, Farivar AS, Woolley SM, Merry HE, Wolf PS, Mackinnon-Patterson B, Keech JC, Fitzsullivan E, Mulligan MS . Alveolar macrophage secretory products effect type 2 pneumocytes undergoing hypoxia-reoxygenation[J]. Ann Thorac Surg, 2008,86(6):1774-1779
doi: 10.1016/j.athoracsur.2008.07.071
[10] Li L, Huang L, Sung SS, Vergis AL, Rosin DL, Rose CE Jr, Lobo PI, Okusa MD . The chemokine receptors CCR2 and CX3CR1 mediate monocyte/macrophage trafficking in kidney ischemia-reperfusion injury[J]. Kidney Int, 2008,74(12):1526-1537
doi: 10.1038/ki.2008.500
[11] Ko GJ, Boo CS, Jo SK, Cho WY, Kim HK . Macrophages contribute to the development of renal fibrosis following ischemia/reperfusion-induced acute kidney injury[J]. Nephrol Dial Transplant, 2008,23(3):842-852
[12] Kinsey GR, Li L, Okusa MD . Inflammation in acute kidney injury[J]. Nephron Exp Nephrol, 2008,109(4):102-107
doi: 10.1159/000142934 pmid: 18802372
[13] Jang HR, Rabb H . The innate immune response in ischemic acute kidney injury[J]. Clin Immunol, 2009,130(1):41-50
doi: 10.1016/j.clim.2008.08.016 pmid: 2646108
[14] Jo SK, Sung SA, Cho WY, Go KJ, Kim HK . Macrophages contribute to the initiation of ischaemic acute renal failure in rats[J]. Nephrol Dial Transplant, 2006,21(5):1231-1239
doi: 10.1093/ndt/gfk047 pmid: 16410269
[15] Jones SA, Horiuchi S, Topley N, Yamamoto N, Fuller GM . The soluble interleukin 6 receptor: mechanisms of production and implications in disease[J]. FASEB J, 2001,15(1):43-58
doi: 10.1088/0957-4484/12/4/342 pmid: 11149892
[16] Nechemia-Arbely Y, Barkan D, Pizov G, Shriki A, Rose-John S, Galun E, Axelrod JH . IL-6/IL-6R axis plays a critical role in acute kidney injury[J]. J Am Soc Nephrol, 2008,19(6):1106-1115
doi: 10.1681/ASN.2007070744
[17] Domanski L, Pawlik A, Safranow K, Jakubowska K, Dziedziejko V, Chlubek D, Rozanski J, Myslak M, Romanowski M, Sulikowski T, Sienko J, Ostrowski M, Ciechanowski K . Purine and cytokine concentrations in the renal vein of the allograft during reperfusion[J]. Transplant Proc, 2007,39(5):1319-1322
doi: 10.1016/j.transproceed.2006.11.022 pmid: 17580130
[18] Yang X, Sun Y, Xu Q, Guo Z . Synjournal and immunosuppressive activity of L-rhamnopyranosyl flavonoids[J]. Biomol Chem, 2006,4(12):2483-2491
doi: 10.1039/b604521a pmid: 16763696
[19] Lin HQ, Wang JJ, Tang J, Hu HF . Suppressive effect of astilbin on rat lung transplantation rejection[J]. Wei Xun Huan Xue Za Zhi, 2008,18(2):8-9
林慧庆, 王建军, 唐建, 胡海丰 . 落新妇甙对大鼠肺移植排斥反应的抑制作用[J]. 微循环学杂志, 2008,18(2):8-9
[20] Chen T, Pan TC, Gao SH, Li J, Zheng Z . Suppression of cardiac allograft rejection in mice by astilbin[J]. Shandong Yi Yao, 2006,46(33):7-8
陈涛, 潘铁成, 高思海, 李军, 郑智 . 落新妇甙对小鼠心脏移植排斥反应的抑制作用[J]. 山东医药, 2006,46(33):7-8
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