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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (8): 753-757.doi: 10.3736/jcim20090809

• Original Experimental Research • Previous Articles     Next Articles

Protective effects of astilbin on renal ischemia-reperfusion injury in rats

Shao-hua Songa,Xiao-yun Shenb,Fang Liua,Yi Tangb,Zhen-meng Wangc,Zhi-ren Fua   

  1. a Organ Transplantation Center, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
    b Institute of Immunology,Second Military Medical University,Shanghai 200433,China
    c Department of Anesthesiology,Eastern Hepatobiliary Surgery Hospital,Second Military Medical University,Shanghai 200433, China
  • Received:2009-04-20 Accepted:2009-06-15 Online:2009-08-20 Published:2009-08-15
  • Contact: Zhi-ren Fu E-mail:zhirenf@sh163.net

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Objective

To investigate the protective effects of astilbin on renal ischemia-reperfusion (IR) injury in rats. 

Methods

Twenty-four male SD rats, two months old, were randomly allocated into three groups: sham-operated group (n=8), untreated group (n=8) and astilbin group (n=8). Rats in the untreated group and the astilbin group underwent temporary renal artery occlusion to induce IR injury. The rats in the astilbin group were intraperitoneally injected with 12 mg/mL astilbin at a dose of 30 mg/kg from 3 day before IR injury until to be sacrificed once per day, and rats in the untreated group were injected with equal volume of normal saline at the same time. After 6-hour reperfusion, blood urea nitrogen (BUN) and serum creatinine (SCr) and histological changes of the renal tissues were detected to evaluate renal injury. Expressions of monocyte chemoattractant protein-1 (MCP-1) mRNA and protein in the renal tissues and the serum contents of interleukin-6 (IL-6) and IL-1β were also measured with semi-quantitative reverse transcription-polymerase chain reaction, Western blotting or enzyme-linked immunosorbent assay.
Results

Compared with the untreated group, BUN and SCr levels were significantly decreased in the astilbin group after 6-hour reperfusion (P<0.01), and similar results were also found in histological examination. The expressions of MCP-1 mRNA and protein in renal tissues in the astilbin group were lower than those in the untreated group. The serum contents of IL-6 and IL-1β were decreased in the astilbin group as compared with the untreated group (P<0.01). 

Conclusion

Astilbin can ameliorate kidney IR injury in rats by inhibiting the production of chemokine MCP-1 and cytokines IL-6 and IL-1β.

Key words: Astilbin, Reperfusion injury, Kidney, Rats

Table 1

Contents of BUN and SCr in different groups after 6-hour reperfusion （?x±s）"

Group n BUN (mmol/L) SCr (μmol/L)
Sham-operated 8 7.0±0.4 69.3±5.8
Untreated 8 21.3±2.0** 215.4±18.7**
Astilbin 8 13.0±1.3△△ 122.3±10.0△△

Figure 1

Histological changes of renal tissues in different groups after 6-hour reperfusion observed by HE staining (Light microscopy, ×200)"

Table 2

Histological score of renal tubular injury and expression of MCP-1 mRNA in renal tissues in different groups after 6-hour reperfusion （?x±s）"

Group n Score of renal tubule injury Relative MCP-1 mRNA level
Sham-operated 8 16±6 0.26±0.06
Untreated 8 428±58** 1.69±0.15**
Astilbin 8 226±36△△ 0.38±0.05△△

Figure 2

Expression of MCP-1 protein in renal tissues in different groups after 6-hour reperfusion"

Table 3

Serum contents of IL-6 and IL-1β in different groups after 6-hour reperfusion ($\bar{x}$±s, ng/L)"

Group n IL-6 IL-1β
Sham-operated 8 10.6±1.9 27.7±6.2
Untreated 8 144.5±16.2** 291.7±22.7**
Astilbin 8 45.7±7.6△△ 161.7±17.8△△
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