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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (8): 774-780.doi: 10.3736/jcim20100808

• Original Experimental Research • Previous Articles     Next Articles

An extract of the oyster mushroom, Pleurotus ostreatus, increases catalase gene expression and reduces protein oxidation during aging in rats

Thanasekaran Jayakumara,Philip Aloysius Thomasb,Mathivanan Isaia,Pitchairaj Geraldinea   

  1. a Department of Animal Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India
    b Institute of Ophthalmology, Joseph Eye Hospital, Tiruchirappalli 620 001, Tamil Nadu, India
  • Received:2010-04-09 Accepted:2010-05-18 Online:2010-08-20 Published:2010-08-15

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Objective

The objective of the present study was to address the effect of mushroom Pleurotus ostreatus on the catalase (CAT) gene expression and the protein carbonyls in liver and kidney of aged (24 months old) rats.
Methods

Eighteen acclimated rats were divided into 3 groups of 6 each: groupⅠ, normal young (4 months old) rats; group Ⅱ, normal aged (24 months old) untreated rats; group Ⅲ, normal aged rats treated with mushroom P. ostreatus extract (200 mg/kg body weight administered intraperitoneally for 30 days). On the 31st day, rats were sacrificed by decapitation; the livers and kidneys were removed, washed free of blood, blotted dry and processed immediately. Reverse transcriptase-polymerase chain reaction (RT-PCR) and spectrophotometry were utilized for the analyses of CAT gene expression and protein carbonyl content in the tissues of livers and kidneys.
Results

In aged rats that had been treated with the extract of P. ostreatus (group Ⅲ), the level of the transcript of CAT gene was found to be higher than that in liver (P<0.01) and kidney (P<0.05) of aged untreated (group Ⅱ) rats, respectively. Treatment of aged rats with P. ostreatus extract (group Ⅲ) resulted in protein carbonyl levels being significantly lower in liver (P<0.05) and kidney (P<0.01) than those observed in aged untreated (group Ⅱ) rats.
Conclusion

These results suggest that an extract of P. ostreatus can enhance the antioxidant enzyme (CAT) gene expression and could decrease the incidence of free radical-induced protein oxidation in aged rats, thereby protecting the occurrence of age-associated disorders that involve free radicals.

Key words: Pleurotus ostreatus, Catalase, Reactive oxygen species, Reverse transcriptase-polymerase chain reaction, Protein carbonyl, Rats

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Figure 1

Catalase gene transcripts in the liver and kidney of different groups of rats"

Figure 2

Agarose gel electrophoresis of RT-PCR products from total RNA isolated by using TRIzol M: 100 bp DNA ladder; L1: Liver of young rats; L2: Liver of aged rats; L3: Liver of aged rats treated with mushroom extract; L4: Kidney of young rats; L2: Kidney of aged rats; L3: Kidney of aged rats treated with mushroom extract."

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