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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (8): 785-789.doi: 10.3736/jcim20100810

• Original Experimental Research • Previous Articles     Next Articles

Effects of medicinal extract for tonifying kidney to relieve asthma on glucocorticoid receptor expression in lung tissues of rats with bronchial asthma

 Ke-hua Shia, Yin-gen Wub, Bi-dan Xionga, Xiao Yua   

  1. a Department of Respiratory Diseases, Shanghai Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, China
    b Department of Respiratory Diseases, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2010-01-27 Accepted:2010-04-04 Online:2010-08-20 Published:2010-08-15

Objective

To observe the effects of medicinal extract for tonifying kidney to relieve asthma on glucocorticoid receptor (GR) expression in rats with asthma, and to explore its mechanism in treating asthma.
Methods

Sixty SD rats were randomly divided into normal control group, untreated group, dexamethasone group, and medicinal extract-prevented, medicinal extract-treated, and medicinal extract-prevented and -treated groups, with ten rats in each group. Asthma was induced by intraperitoneal injection of ovalbumin (OVA) and forced inhalation of atomized OVA. Expression of GR in lung tissues was detected by immunohistochemical method. Pathological changes of the lung tissues were observed by HE straining.
Results

Expression of GR was lower in the untreated group than in the normal control group (P<0.05). Expressions of GR in medicinal extract groups were up-regulated as compared with those in the untreated group and dexamethasone group (P<0.05, P<0.01), and there were no significant differences as compared with the normal control group (P>0.05).
Conclusion

Medicinal extract for tonifying kidney to relieve asthma can increase the expression of GR in lung tissues of asthmatic rats, which may be one of its mechanisms in preventing and treating asthma.

Key words: Medicinal extract, Reinvigorating kidney, Antiasthma, Asthma, Glucocorticoid receptor, Rats

Figure 1

Pathological change of lung tissues in rats with asthma observed by HE staining (Light microscopy, ×100) A: Normal control group; B: Untreated group; C: Dexamethasone group; D: Medicinal extract-prevented group; E: Medicinal extract-treated group; F: Medicinal extract-prevented and -treated group."

Table 1

Immunohistochemical index of GR expression in lung tissues of rats with asthma in different groups"

Figure 2

Expression of GR in lung tissues of rats with asthma observed by immunohistochemical method (Light microscopy, ×100) A: Normal control group; B: Untreated group; C: Dexamethasone group; D: Medicinal extract-prevented group; E: Medicinal extract-treated group; F: Medicinal extract-prevented and -treated group."

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