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Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (1): 64-69.doi: 10.3736/jcim20110111

• Original Experimental Research • Previous Articles     Next Articles

Effects of rat serum containing Chinese herbal medicine Sangen Decoction on osteoclastogenesis and bone resorption of osteoclasts induced by polymethylmethacrylate particles

Shu-qiang Wang1,2, Wu Rao1, Lian-fu Deng3, Ya-ping Zhu3, Yong-qiang Chen1()   

  1. 1. Department of Orthopaedics and Traumatology, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, China
    2. Department of Orthopaedics and Traumatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
    3. Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
  • Received:2010-08-02 Accepted:2010-08-13 Online:2011-01-20 Published:2011-01-15
  • Contact: Chen Yong-qiang E-mail:chenyongqiang@medmail.com.cn

Objective: To investigate the effects of Sangen Decoction, a compound Chinese herbal medicine, on osteoclastogenesis and bone resorption function of osteoclasts induced by polymethylmethacrylate particles in vitro.
Methods: Macrophage colony stimulating factor (M-CSF) and receptor activator of nuclear factor-κB ligand (RANKL) were used to induce differentiation of bone marrow-derived macrophages (BMMs) towards osteoclasts. BMMs and polymethylmethacrylate particles with ratio of 1︰3 were added to the 24-well plate and 96-well plate with bone slices respectively. A total of 50 male SD rats were divided into 5 groups randomly with each group containing 10 rats. After being treated with different drugs, serum samples of rats in each group were extracted, i.e., the blank serum, Western medicine (ibandronate) serum and high-, medium-, and low-dose Sangen Decoction serum and were added to the medium respectively. The tartrate-resistant acid phosphatase (TRAP) staining was used to identify the differentiation of BMMs and for counting of osteoclasts. Area of lacuna induced by osteoclast bone resorption on the bone slices was measured by computer image processing.
Results: Numbers of osteoclasts of treatment groups were less than that of blank group by TRAP staining (P<0.05); numbers of osteoclasts of positive control group and high-dose Sangen Decoction group were much lower than those of medium- and low-dose Sangen Decoction groups (P<0.05), and no difference was found between Western medicine group and high-dose Sangen Decoction group (P>0.05). In bone resorption assay, area of lacuna of blank group was larger than those of treatment groups (P<0.05); areas of lacuna of Western medicine group and high-dose Sangen Decoction group were much smaller than those of medium- and low-dose Sangen Decoction groups (P<0.05), and no difference was found between Western medicine group and high-dose Sangen Decoction group (P>0.05).
Conclusion: Sangen Decoction can inhibit osteoclastogenesis induced by polymethylmethacrylate particles as well as bone resorption function of osteoclasts.

Key words: compounds (TCD), osteoclasts, bone resorption, osteolysis, Sangen Decoction

Figure 1

Tartate-resistant acid phosphatase staining of osteoclasts (Light microscopy, ×100)A: Blank group; B: Ibandronate group; C: High-dose Sangen Decoction group; D: Medium-dose Sangen Decoction group; E: Low-dose Sangen Decoction group."

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Figure 2

Resorption assays for osteoclasts (Light microscopy, ×200) A: Blank group; B: Ibandronate group; C: High-dose Sangen Decoction group; D: Medium-dose Sangen Decoction group; E: Low-dose Sangen Decoction group"

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