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Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (1): 64-69.doi: 10.3736/jcim20110111

• Original Experimental Research • Previous Articles     Next Articles

Effects of rat serum containing Chinese herbal medicine Sangen Decoction on osteoclastogenesis and bone resorption of osteoclasts induced by polymethylmethacrylate particles

Shu-qiang Wang1,2, Wu Rao1, Lian-fu Deng3, Ya-ping Zhu3, Yong-qiang Chen1()   

  1. 1. Department of Orthopaedics and Traumatology, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, China
    2. Department of Orthopaedics and Traumatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
    3. Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
  • Received:2010-08-02 Accepted:2010-08-13 Online:2011-01-20 Published:2011-01-15
  • Contact: Chen Yong-qiang E-mail:chenyongqiang@medmail.com.cn

Objective: To investigate the effects of Sangen Decoction, a compound Chinese herbal medicine, on osteoclastogenesis and bone resorption function of osteoclasts induced by polymethylmethacrylate particles in vitro.
Methods: Macrophage colony stimulating factor (M-CSF) and receptor activator of nuclear factor-κB ligand (RANKL) were used to induce differentiation of bone marrow-derived macrophages (BMMs) towards osteoclasts. BMMs and polymethylmethacrylate particles with ratio of 1︰3 were added to the 24-well plate and 96-well plate with bone slices respectively. A total of 50 male SD rats were divided into 5 groups randomly with each group containing 10 rats. After being treated with different drugs, serum samples of rats in each group were extracted, i.e., the blank serum, Western medicine (ibandronate) serum and high-, medium-, and low-dose Sangen Decoction serum and were added to the medium respectively. The tartrate-resistant acid phosphatase (TRAP) staining was used to identify the differentiation of BMMs and for counting of osteoclasts. Area of lacuna induced by osteoclast bone resorption on the bone slices was measured by computer image processing.
Results: Numbers of osteoclasts of treatment groups were less than that of blank group by TRAP staining (P<0.05); numbers of osteoclasts of positive control group and high-dose Sangen Decoction group were much lower than those of medium- and low-dose Sangen Decoction groups (P<0.05), and no difference was found between Western medicine group and high-dose Sangen Decoction group (P>0.05). In bone resorption assay, area of lacuna of blank group was larger than those of treatment groups (P<0.05); areas of lacuna of Western medicine group and high-dose Sangen Decoction group were much smaller than those of medium- and low-dose Sangen Decoction groups (P<0.05), and no difference was found between Western medicine group and high-dose Sangen Decoction group (P>0.05).
Conclusion: Sangen Decoction can inhibit osteoclastogenesis induced by polymethylmethacrylate particles as well as bone resorption function of osteoclasts.

Key words: compounds (TCD), osteoclasts, bone resorption, osteolysis, Sangen Decoction

Figure 1

Tartate-resistant acid phosphatase staining of osteoclasts (Light microscopy, ×100)A: Blank group; B: Ibandronate group; C: High-dose Sangen Decoction group; D: Medium-dose Sangen Decoction group; E: Low-dose Sangen Decoction group."

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Figure 2

Resorption assays for osteoclasts (Light microscopy, ×200) A: Blank group; B: Ibandronate group; C: High-dose Sangen Decoction group; D: Medium-dose Sangen Decoction group; E: Low-dose Sangen Decoction group"

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[1] Allen DJ, Beaulé PE . Rationale for metal-on-metal total hip replacement[J]. J Surg Orthop Adv, 2008,17(1):6-11
[2] Millett PJ, Allen MJ , Bostrom MP.Effects of alendronate on particle-induced osteolysis in a rat model[J].J Bone Joint Surg Am, 2002, 84- A(2):236-249
[3] Pomerantz RG, Mody E, Husni ME, Qureshi AA . Follow-up of psoriatic arthritis mutilans patients treated with anti-TNF-alpha therapy[J]. J Drugs Dermatol, 2009,8(4):406-412
[4] Shen PA. Pharmacology and clinical use of traditional Chinese herbal medicines[M]. Beijing: People’s Medical Publishing House, 2006: 420-421
沈丕安 . 中药药理与临床应用[M]. 北京: 人民卫生出版社, 2006: 420-421
[5] Bradley EW, Oursler MJ . Osteoclast culture and resorption assays[J]. Methods Mol Biol, 2008,455:19-35
doi: 10.1007/978-1-59745-104-8
[6] Zhang H, Ricciardi BF, Yang X, Shi Y, Camacho NP, Bostrom MG . Polymethylmethacrylate particles stimulate bone resorption of mature osteoclasts in vitro[J]. Acta Orthop, 2008,79(2):281-288
doi: 10.1080/17453670710015166
[7] Bar-Shavit Z . The osteoclast: a multinucleated, hematopoietic-origin, bone-resorbing osteoimmune cell[J]. J Cell Biochem, 2007,102(5):1130-1139
doi: 10.1002/(ISSN)1097-4644
[8] Schwarz EM . What potential biologic treatments are available for osteolysis[J]. J Am Acad Orthop Surg, 2008,16:S72-S75
doi: 10.5435/00124635-200800001-00015
[9] Fehm T, Beck V, Banys M, Lipp HP, Hairass M, Reinert S, Solomayer EF, Wallwiener D, Krimmel M . Bisphosphonate-induced osteonecrosis of the jaw(ONJ): Incidence and risk factors in patients with breast cancer and gynecological malignancies[J]. Gynecol Oncol, 2009,112(3):605-609
doi: 10.1016/j.ygyno.2008.11.029
[10] Miyazaki M, Fujikawa Y, Takita C, Tsumura H . Tacrolimus and cyclosporine A inhibit human osteoclast formation via targeting the calcineurin-dependent NFAT pathway and an activation pathway for c-Jun or MITF in rheumatoid arthritis[J]. Clin Rheumatol, 2007,26(2):231-239
[11] Chen BY, Liang GL, Mei QC . Isolation and identification of two effective compositions from the roots of Cargana sinica and study on its physiological activities[J]. Fudan Xue Bao Yi Xue Ban, 2005,32(6):721-724
陈步云, 梁高林, 梅其春 . 金雀根中两种有效成分的分离鉴定及其生理活性测定[J]. 复旦学报(医学版), 2005,32(6):721-724
[12] Wang HW, Ni Q, Lin L . Research progress on serum containing Chinese herbs and application in traditional Chinese medicine[J]. Beijing Zhong Yi Yao, 2008,27(9):698-701
王洪武, 倪青, 林兰 . 中药含药血清的研究进展及其在中医学中的应用[J]. 北京中医药, 2008,27(9):698-701
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