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Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (3): 313-319.doi: 10.3736/jcim20110313

• Original Experimental Research • Previous Articles     Next Articles

Effects of Chinese herbal medicine Xuefu Zhuyu Decoction on angiotensin Ⅱ-induced proliferation and extracellular matrix synthesis of rat cardiac fibroblasts

Yan Shen(), Hong Wei, Chun-lan Jin   

  1. Department of Cardiology, Yueyang Hospital of Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
  • Received:2010-12-13 Accepted:2011-01-04 Online:2011-03-20 Published:2011-03-15
  • Contact: Shen Yan E-mail:sheny26@126.com

Objective: To investigate the effects of Xuefu Zhuyu Decoction, a compound Chinese herbal medicine, on the proliferation and extracellular matrix synthesis of cardiac fibroblasts (CFs) from neonatal rats induced by angiotensin Ⅱ, and to explore the mechanism of Xuefu Zhuyu Decoction in improving myocardial fibrosis.
Methods: CFs derived from neonatal rats were cultured with enzymatic dissociation to establish a cell model of cardiac fibrosis induced by angiotensin Ⅱ in vitro. CFs were divided into 6 groups, including blank control group, angiotensin Ⅱ group, valsartan-medicated serum group, and 5%, 10% and 15% Xuefu Zhuyu decoction-medicated serum group. CFs in the blank control group were incubated with normal rat serum; CFs in the other groups were stimulated with angiotensin Ⅱ at a dose of 10 –6 mol/L, and then incubated with normal rat serum and 5%, 10% and 15% Xuefu Zhuyu decoction-medicated serum for 24 h, respectively. Methyl thiazolyl tetrazolium assay was employed to measure CF proliferation, and collagen synthesis was detected by analysis of the hydroxyproline in CFs. Radioimmunoassay was used to measure concentrations of procollagen Ⅲ and hyaluronic acid. Enzyme-linked immunosorbent assay was used to measure fibronectin expression.
Results: Compared with the blank control group, angiotensin Ⅱ induced cardiac fibroblast proliferation, stimulated DNA and collagen syntheses (P<0.01), and increased fibronectin, hyaluronic acid and procollagen Ⅲ concentrations (P<0.01). Xuefu Zhuyu decoction-medicated serum inhibited CF proliferation and DNA and collagen syntheses (P<0.05), and decreased the concentrations of fibroectin, hyaluronic acid and procollagen Ⅲ (P<0.01).
Conclusion: Angiotensin Ⅱ can induce cardiac fibrosis, stimulate cardiac fibroblast proliferation and extracellular matrix synthesis, while Xuefu Zhuyu Decoction can prevent cardiac fibrosis, the mechanism of which may be related to inhibiting CF proliferation and extracellular matrix synthesis.

Key words: cardiac fibrosis, cardiac remodeling, drugs,Chinese herbal, angiotensin H, extracellular matrix

Figure 1

Morphology and identification of cardiac fibroblasts A: Fibroblasts were fusiform and polygonal and observed by hematoxylin and eosin staining under an inverted microscope (×100); B: Fibroblasts were negative for α-smooth muscle actin and observed by EnVision under an inverted microscope (×200); C: Fibroblasts were positive for fibroectin expression and observed by EnVision method under an inverted microscope (×200); D: Fibroblasts were positive for vitronectin and observed by EnVision method under an inverted microscope (×200)."

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[1] Guo ZK. Modern heart histology[M]. Beijing: People's Medical Publishing House, 2007
郭志坤 . 现代心脏组织学[M]. 北京: 人民卫生出版社, 2007
[2] Wang XP, Wang Q . Modified Xuefu Zhuyu Decoction in treating 104 cases of angina pectoris[J]. Zhongguo Yi Yao Zhi Nan, 2010,8(6):80-81
doi: 10.3969/j.issn.1671-8194.2010.06.050
王秀萍, 王琦 . 血府逐瘀汤加味治疗冠心病心绞痛104例[J]. 中国医药指南, 2010,8(6):80-81
doi: 10.3969/j.issn.1671-8194.2010.06.050
[3] Zhu XD, Liu YL . Xuefu Zhuyu Decoction in treating 130 cases of myocardial ischemia of coronary heart disease[J]. Zhongguo Zhong Yi Yao Xian Dai Yuan Cheng Jiao Yu, 2009,7(12):32-33
doi: 10.3969/j.issn.1672-2779.2009.12.025
朱旭东, 刘友伦 . 血府逐瘀汤治疗冠心病心肌缺血130例[J]. 中国中医药现代远程教育, 2009,7(12):32-33
doi: 10.3969/j.issn.1672-2779.2009.12.025
[4] Zhang SW, Xian SX, Yang ZQ, Zhang KS . Clinical research of Xuefu Zhuyu Decoction on the intervention of myocardial fibrosis of coronary heart disease with blood stasis syndrome[J]. Guangzhou Zhong Yi Yao Da Xue Xue Bao, 2009,26(1):13-15
doi: 10.3969/j.issn.1007-3213.2009.01.004
张双伟, 冼绍祥, 杨忠奇, 张开升 . 血府逐瘀汤干预冠心病心血瘀阻证心肌纤维化的临床研究[J]. 广州中医药大学学报, 2009,26(1):13-15
doi: 10.3969/j.issn.1007-3213.2009.01.004
[5] Hu SY, Zhang GH, Xian SX, Zhao LC, Wu YH, Qi YH . Effects of Tianma Gouteng Decoction, Xuefu Zhuyu Decoction and Wendan Decoction on proliferation of SHR cardiac fibroblasts[J]. Guangdong Yi Xue, 2009,30(2):196-198
doi: 10.3969/j.issn.1001-9448.2009.02.014
胡世云, 张国华, 冼绍祥, 赵立诚, 伍耀衡, 齐玉焕 . 天麻钩藤饮和血府逐瘀汤与温胆汤含药血清对SHR心肌成纤维细胞增殖的影响[J]. 广东医学, 2009,30(2):196-198
doi: 10.3969/j.issn.1001-9448.2009.02.014
[6] Ding ZS, Gao CX, Ji RR, Wo XD . Effect of Xuefu Zhuyu Decoction on collagen synjournal and proliferation of cardiac fibroblasts[J]. Zhong Yao Cai, 2002,25(7):481-483
丁志山, 高承贤, 吉瑞瑞, 沃兴德 . 血府逐瘀汤对心肌成纤维细胞增殖和胶原合成的影响[J]. 中药材, 2002,25(7):481-483
[7] Zhang DM, Qin Y, Lü XY, Wu AM, Zhu LQ, Wang SR, Jiang LD . Effects of tetramethylpyrazine on angiotensin Ⅱ-induced proliferation and type Ⅰ collagen synjournal of rat cardiac fibroblasts[J]. J Chin Integr Med, 2009,7(3):232-236
doi: 10.3736/jcim20090307
张冬梅, 秦英, 吕浠滢, 吴爱明, 朱陵群, 王硕仁, 姜良铎 . 川芎嗪对血管紧张素Ⅱ诱导的大鼠心肌成纤维细胞增殖及Ⅰ型胶原合成的影响[J]. 中西医结合学报, 2009,7(3):232-236
doi: 10.3736/jcim20090307
[8] Hou XL, Li BL, Zhao L, Huang SD, Xu ZY, Zhang GX . Effects of Xuefu Zhuyu Capsule on endothelin-1 release in myocardium and vascular endothelium and nitric oxide/nitric oxide synthase system of swines after acute myocardial infarction and reperfusion[J]. J Chin Integr Med, 2008,6(4):381-386
侯霄雷, 李白翎, 赵雷, 黄盛东, 徐志云, 张冠鑫 . 血府逐瘀胶囊对急性心肌梗死再灌注后心肌及内皮素-1、一氧化氮/一氧化氮合酶体系影响的实验研究[J]. 中西医结合学报, 2008,6(4):381-386
[9] Situ ZQ, Wu JZ. Cell culture[M]. Beijing: World Publishing Corporation, 2004: 3
司徒镇强, 吴军正 . 细胞培养[M]. 北京: 世界图书出版公司, 2004: 3
[10] Fedak PW, Verma S, Weisel RD, Li RK . Cardiac remodeling and failure: from molecules to man(Part Ⅰ)[J]. Cardiovasc Pathol, 2005,14(1):1-11
doi: 10.1016/j.carpath.2004.12.002
[11] Sun Y, Weber KT . Infarct scar: a dynamic tissue[J]. Cardiovasc Res, 2000,46(2):250-256
doi: 10.1016/S0008-6363(00)00032-8 pmid: 10773228
[12] Lin YH, Shiau YC, Yen RF, Lin LC, Wu CC, Ho YL, Huang PJ . The relation between myocardial cyclic variation of integrated backscatter and concentrations of procollagen propeptides in hypertensive patients[J]. Ultrasound Med Biol, 2004,30(7):885-891
doi: 10.1016/j.ultrasmedbio.2004.04.007
[13] Brilla CG . Renin-angiotension-aldosterone system and myocardial fibrosis[J]. Cardiovasc Res, 2000,47(1):1-3
doi: 10.1016/S0008-6363(00)00092-4 pmid: 10869522
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